Probiotic Protects Kidneys Exposed to Microcystin-LR DOI Creative Commons

Yolanda Hu,

Irene Sun,

Eric Tang

et al.

Translation The University of Toledo Journal of Medical Sciences, Journal Year: 2024, Volume and Issue: 12(1)

Published: Jan. 29, 2024

Cyanobacterial Harmful Algal Blooms (CyanoHABs) occur when colonies of photosynthetic bacteria called cyanobacteria grow out control, usually in warm, nutrient-rich, slow-moving waters. They are becoming increasingly prevalent around the world and release harmful toxins cyanotoxins into bodies water, which negatively affect human ecological health. One such cyanotoxin is microcystin, with microcystin-leucine arginine (MC-LR) being most widespread. Exposure to MC-LR inhibits serine threonine protein phosphatase 1 2A humans, causing a myriad health problems. Fortunately, certain naturally occurring may be able degrade reverse its effects. Mice were separated five experimental groups based on three types pre-treatments (control drinking water/vehicle, probiotic-supplemented heat-inactivated water) as well two exposures (microcystin-LR water/vehicle). RNA was extracted from kidneys for sequencing because exacerbates kidney disease. Gene expression data analyzed 3 Pod Reports, an R package that produces three-part report consisting Set Enrichment Analysis (GSEA), EnrichR, integrative LINCS (iLINCS). exposure associated upregulated cellular respiration metabolism pathways downregulated transcription pathways. Probiotic pre-treatment combined lipoprotein particle ribosome Overall, probiotic mixture corrected transcriptional profile resulting exposure. Future high yield could targeted therapeutic benefit include VEGFR inhibitors increased renal indicator genes EGFR.

Language: Английский

Reimagining water quality assessment: a nutritional indexing perspective for drinking water resources management DOI
Johnson C. Agbasi, Ahmad Alkasimi, Ahmad F. Turki

et al.

International Journal of Environmental & Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 27

Published: April 4, 2025

Language: Английский

Citations

2
Reproductive toxicity of perfluorobutane sulfonate in zebrafish (Danio rerio): Impacts on oxidative stress, hormone disruption and HPGL axis dysregulation DOI

Jenila John Santhi,

Praveen Kumar Issac, Manikandan Velayutham

et al.

Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology, Journal Year: 2025, Volume and Issue: 289, P. 110122 - 110122

Published: Jan. 7, 2025

Language: Английский

Citations

1
The Evaluation of a New ELISA-Based Kit for Total Microcystins as an Early Detection Tool for Microcystin Blooms in Source Waters and Its Application State-Wide to Oregon Source and Finished Drinking Waters DOI Creative Commons
Katie Adams, Kale Clauson,

William A. Adams

et al.

Toxins, Journal Year: 2025, Volume and Issue: 17(2), P. 53 - 53

Published: Jan. 24, 2025

Due to cyanobacterial toxin (cyanotoxin) contamination issues in 2018, the city of Salem, Oregon, issued a 33-day do-not-drink advisory for vulnerable people among 200,000 residents. After incident, state Oregon put place drinking water rules require routine testing raw water, as well finished cases where cyanotoxin concentrations exceeded trigger values. The United States Environmental Protection Agency (EPA) total microcystins health level (HAL) small children is 0.3 µg/L. This equivalent minimum reporting (MRL) EPA Method 546. Consequently, there was no ability provide early warnings via using method. In this study, we performed comparison precision and accuracy enzyme-linked immunosorbent assay (ELISA) described method more sensitive assay, Streptavidin-enhanced Sensitivity (SAES) assay. Based on these studies quantitation limit determinations confirmations, Office Ground Water Drinking (OGWDW) has concluded SAES kit meets requirements With an MRL that one-third original concentration, new provides but critical window identifying warnings. Challenges remain with providing due variability bloom dynamics; however, allowed lower susceptible systems, thereby additional warning.

Language: Английский

Citations

1
Intrinsic interaction inferred oxidative stress and apoptosis by Biosurfactant-microplastic hybrid reduces coordinated in vivo biotoxicity in zebrafish (Danio rerio). DOI Creative Commons

Utsa Saha,

Aishee Ghosh,

Adrija Sinha

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101466 - 101466

Published: Jan. 8, 2025

The proliferation of microplastics (μP) in aquatic environments poses a significant threat to ecosystem health, with repercussions extending organisms and potentially human health. In this study, we investigated the efficacy novel biosurfactant-microplastic (BSμP) hybrid reducing vivo green bio-toxicity induced by oxidative stress apoptosis zebrafish (Danio rerio). Microplastics, ubiquitous environments, were hybridised Biosurfactant evaluate their potential mitigating effects. A stable BSμP was formed zeta -10.3 ± 1.5 mV. Exposure embryos μP resulted increased markers, including elevated levels reactive oxygen species apoptosis, as evidenced expression apoptotic markers morphological changes embryonic zebrafish. However, significantly ameliorated observed toxic effects reduced activity. This effect deduced intrinsic hybridisation, which likely mitigated bioavailability toxicity molecular interaction metabolic proteins like Sod1 p53 through less accumulation internalisation. Overall, our findings highlight promising approach for ecological impacts microplastic pollution.

Language: Английский

Citations

0
Coumarin‐triazole hybridized as therapeutic‐based niosomes for advanced drug release of Cefixime against multi‐drug resistance Salmonella enterica DOI Open Access
Samiullah Burki, Imdad Ali, Zeba Gul Burki

et al.

Journal of Surfactants and Detergents, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Abstract Bacterial resistance to conventional antibiotics has become a massive problem for healthcare systems. This could result in serious consequences treating bacterial infections, including treatment failure. In turn, drug promoted the development of more effective antibacterial agents. Niosomes are considered efficient agents enhancement activity antibiotics. Herein, Coumarin‐triazole hybrid (CAT) niosomes were developed and evaluated against extensively drug‐resistant Salmonella enterica (XDR S. ). The Cefixime‐loaded CAT niosomal vesicle average particle size was 342 ± 3 nm, with zeta potential −28 2.0 mV. vesicles showed prominent entrapment efficiency 74% 3%. A blood hemolytic assay revealed no hemolysis occurred. exhibited reduced cytotoxic effect NIH/3T3 normal mouse fibroblast cells, at maximum concentration 1000 μg/mL. However, MIC 50 empty Cefixime loaded XDR observed 250 125 μgmL −1 , respectively, indicating that lowered by two‐fold. Topographical atomic force microscopy (AFM) images exposure efficiently damaged cell membrane resulting leaking scattering.

Language: Английский

Citations

0
Preparation and characterization of multilayered microcapsules of Lactobacillus rhamnosus encapsulated with sodium alginate, hyaluronic acid and carrageenan and their protective effects on the retina DOI
Xiaodong Wang, Wenqing Lv, Chunxue Zhai

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 141104 - 141104

Published: Feb. 1, 2025

Language: Английский

Citations

0
Silica dust exposure and associated pulmonary dysfunction among mine workers DOI
Aima Iram Batool, R. Shaheen,

Naima Huma Naveed

et al.

Journal of Environmental Science and Health Part A, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 11

Published: Feb. 28, 2025

This study assessed the impact of silica exposure on 145 mine workers in Mianwali, Punjab, Pakistan, compared to 45 non-exposed individuals. Pulmonary function tests revealed significantly reduced lung exposed (P < 0.05), with declines Forced Expiratory Volume one second (FEV1), Vital Capacity (FVC), FEV1/FVC ratio, Peak Flow, and Flow at 25-75% FVC (FEF25-75). Radiological evaluations confirmed extensive damage including pleural effusion, reticular shadowing, consolidation. Oxidative stress markers demonstrated increased lipid peroxidation, Fenton's Stress, Stress Index along antioxidant enzyme activities, Catalase, Superoxide Dismutase, Total Antioxidant Capacity, Glutathione Peroxidase. Hematological analysis showed elevated White Blood Cells, Lymphocyte percentage, Hemoglobin, Hematocrit, Mean Corpuscular Volume, Hemoglobin reflecting systemic inflammation. Silica's piezoelectric properties contributed oxidative cellular damage, exacerbating pulmonary dysfunction. These findings highlight as a severe occupational hazard, causing irreversible impairment imbalance. Implementing strict safety protocols, personal protective measures, regular health monitoring is crucial safeguarding workers.

Language: Английский

Citations

0
Protective Effects of KC14 Peptide from Cyprinus carpio on Copper Sulfate-Induced Toxicity in Zebrafish Larvae: Insights into Anti-inflammatory Cytokine and Glutathione Modulations DOI
Madhumitha Vijayanand, Praveen Kumar Issac, Manikandan Velayutham

et al.

International Journal of Peptide Research and Therapeutics, Journal Year: 2025, Volume and Issue: 31(3)

Published: March 8, 2025

Language: Английский

Citations

0
Neem Azadirachta indica extract augments serum, mucus innate immunity, antioxidant status, and resistance to Aeromonas hydrophila in Rohu Labeo rohita DOI Creative Commons
Saira Naz, Gaurav Sanghvi, Saima Majeed

et al.

North American Journal of Aquaculture, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

Abstract Objective In aquaculture, medicinal plants and their derivatives serve as potent natural boosters for the immune system effective stress relievers aquatic organisms. This study explored how neem plant (NP) Azadirachta indica leaves extract influenced antioxidant capacity of Rohu Labeo rohita. Methods The sample included 240 healthy fingerlings, averaging 22.54 ± 0.82 g in weight, divided randomly into four groups, each containing triplicates 20 fish per glass aquarium. control group (NP0) received a standard diet without additives, whereas other groups were fed NP-infused diets at concentrations 2 g/kg (NP2), 4 (NP4), 6 (NP6) over 60-d period. After this feeding trial, exposed to Aeromonas hydrophila assess response survival rates calculated. Results findings revealed that relative group, NP4 exhibited significantly (P &lt; 0.05) higher levels lysozyme activity both serum mucus. Peroxidase was elevated NP6 although difference not statistically significant. However, peroxidase mucus group. Additionally, immunoglobulin, total protein, ACH50 considerably protease, protein Enzymatic activities such catalase, superoxide dismutase, glutathione dehydrogenase than control. rate followed by NP4. Conclusions conclusion, suggest NP holds potential immunostimulant aquaculture.

Language: Английский

Citations

0
Preliminary Assessment of Essential and Potentially Toxic Elements in the Most Common Spices in a Developing Country: Health Hazard Implication DOI
Md. Saiful Islam, Uttam Biswas Antu,

Ruma Akter

et al.

Biological Trace Element Research, Journal Year: 2025, Volume and Issue: unknown

Published: May 3, 2025

Language: Английский

Citations

0