Genetic assessment of the effect of red yeast (Sporidiobolus pararoseus) as a feed additive on mycotoxin toxicity in laying hens DOI Creative Commons
Shahrbanou Hosseini, Bertram Brenig,

Sunattinee Winitchakorn

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Sept. 6, 2023

Toxic fungal species produce hazardous substances known as mycotoxins. Consumption of mycotoxin contaminated feed and food causes a variety dangerous diseases can even lead to death animals humans, raising global concerns for adverse health effects. To date, several strategies have been developed counteract with contamination. Red yeast novel biological dietary agent is promising strategy eliminate mycotoxicity in living organisms. Poultry are most susceptible contamination, they fed mixture grains at higher risk co-exposure multiple toxic substances. Therefore, this study investigated the genetic mechanism underlying long-term feeding red supplementation interaction mycotoxins using transcriptome profiling (RNA_Seq) liver laying hens. The results showed high number significantly differentially expressed genes chicken diet mycotoxins, whereas Significantly was considerably reduced when supplemented yeast. expression involved phase I (CYP1A1, CYP1A2) II (GSTA2, GSTA3, MGST1) detoxification process downregulated diet, indicating suppression mechanisms. However, antioxidant defense (GSTO1), apoptosis (DUSP8), tumor suppressor (KIAA1324, FBXO47, NME6) were upregulated mycotoxins-exposed animals, suggesting activation response mycotoxicity. Similarly, none hens diet. neither nor exposed feed, decreases adsorption biologically active We conclude that act binder decrease be used an effective poultry industry effects increase safety human consumers.

Language: Английский

Pathological consequences, metabolism and toxic effects of trichothecene T-2 toxin in poultry DOI Creative Commons
Júlia Vörösházi, Zsuzsanna Neogrády, Gábor Mátis

et al.

Poultry Science, Journal Year: 2024, Volume and Issue: 103(3), P. 103471 - 103471

Published: Jan. 17, 2024

Contamination of feed with mycotoxins has become a severe issue worldwide. Among the most prevalent trichothecene mycotoxins, T-2 toxin is particular importance for livestock production, including poultry posing significant threat to animal health and productivity. This review article aims comprehensively analyze pathological consequences, metabolism, toxic effects in poultry. Trichothecene primarily produced by Fusarium species, are notorious their potent toxicity. exhibits broad spectrum negative on leading substantial economic losses as well concerns about welfare food safety modern agriculture. exposure easily results consequences gastrointestinal tract, parenchymal tissues like liver (as key organ its metabolism), kidneys, or reproductive organs. In addition, it also intensely damages immune system-related such spleen, bursa Fabricius, thymus causing immunosuppression increasing susceptibility animals infectious diseases, making immunization programs less effective. The cellular processes transcriptional translational levels induces apoptosis through activation numerous signaling cascades. Furthermore, according recent studies, besides direct abovementioned processes, production reactive molecules free radicals resulting oxidative distress concomitantly occurring damage. conclusion, this provides complex detailed overview mechanism action immunomodulatory stress-related toxin. Understanding these crucial developing strategies mitigate impact avian future.

Language: Английский

Citations

16
Meta-Analysis of the Effects of Yeast Cell Wall Extract Supple-Mentation during Mycotoxin Challenges on the Performance of Laying Hens DOI Creative Commons

Alexandra C. Weaver,

Daniel M. Weaver, Nicholas M. Adams

et al.

Toxins, Journal Year: 2024, Volume and Issue: 16(4), P. 171 - 171

Published: March 30, 2024

A random-effects meta-analysis was conducted to investigate the effect of mycotoxins (MT) without or with inclusion yeast cell wall extract (YCWE, Mycosorb®, Alltech, Inc., Nicholasville, KY, USA) on laying hen performance. total 25 trials were collected from a literature search, and data extracted 8 these that met criteria, for 12 treatments 1774 birds. Laying hens fed MT had lower (p < 0.05) body weight (BW) by −50 g, egg production −6.3 percentage points, −1.95 g than control (CTRL). Inclusion YCWE during mycotoxin challenges (YCWE + MT) resulted in numerically greater = 0.441) BW 12.5 while significantly 0.0001) higher 4.2 points 1.37 respectively. Furthermore, economic assessment calculations indicated may not only support performance but also positive return investment. In conclusion, can play role negatively impacting profitability. feed provided benefits As such, could an important minimizing effects turn aid farm efficiency

Language: Английский

Citations

4
Fumonisins alone or mixed with other fusariotoxins increase the C22–24:C16 sphingolipid ratios in chicken livers, while deoxynivalenol and zearalenone have no effect DOI
Philippe Guerre,

Elodie Lassallette,

Ugo Beaujardin-Daurian

et al.

Chemico-Biological Interactions, Journal Year: 2024, Volume and Issue: 395, P. 111005 - 111005

Published: April 12, 2024

Language: Английский

Citations

3
The Effect of Combined Exposure of Fusarium Mycotoxins on Lipid Peroxidation, Antioxidant Defense, Fatty Acid Profile, and Histopathology in Laying Hens’ Liver DOI Creative Commons
Szabina Kulcsár,

Janka Turbók,

György Kövér

et al.

Toxins, Journal Year: 2024, Volume and Issue: 16(4), P. 179 - 179

Published: April 7, 2024

Fumonisin B1, T-2 toxin, and deoxynivalenol are frequently detected in feed materials. The mycotoxins induce free radical formation and, thereby, lipid peroxidation. effects of mycotoxin exposure at the EU recommended limit (T-2/HT-2 toxin: 0.25 mg/kg; DON = 3AcDON/15-AScDON: 5 fumonisin B1: 20 mg/kg) double dose 0.5 mg/kg, DON/3-AcDON/15-AcDON: 10 mg, FB1: 40 mg/kg feed) were investigated during short-term (3 days) per os liver laying hens. On day 1 higher while on 3 lower MDA concentrations found low-dose group compared to control. Fatty acid composition also changed: proportion monounsaturated fatty acids increased (p < 0.05) polyunsaturated decreased by 3. These alterations resulted a decrease index unsaturation average chain length. Histopathological suggested that incidence severity lesions mycotoxin-treated hens, symptoms correlated with profile total phospholipids. Overall, findings revealed exposure, even EU-recommended limits, induced peroxidation liver, which led changes composition, matched tissue damage.

Language: Английский

Citations

3
The Effects of T-2 Toxin, Deoxynivalenol, and Fumonisin B1 on Oxidative Stress-Related Genes in the Kidneys of Laying Hens DOI Creative Commons
Benjámin Kövesi, Szabina Kulcsár, Zsolt Ancsin

et al.

Toxins, Journal Year: 2024, Volume and Issue: 16(3), P. 154 - 154

Published: March 16, 2024

In the context of nephrotoxic risks associated with environmental contaminants, this study focused on impact mycotoxin exposure renal health laying hens, particular attention to oxidative stress pathways. Sixty hens were assigned three groups—a control group (CON), a low-dose (LOW), and high-dose (HIGH)—and monitored for 72 h. Mycotoxin contamination involved T-2/HT-2 toxin, DON/3-AcDON/15-AcDON, FB1 at their EU-recommended levels (low mix) double doses (high mix). Clinical assessments revealed no signs toxicity or notable weight changes. Analysis glutathione redox system parameters demonstrated that reduced content was lower than in controls 48 h higher Glutathione peroxidase activity increased response exposure. addition, gene expression patterns key redox-sensitive pathways, including Keap1-Nrf2-ARE AhR pathway, examined. Notably, profiles dynamic responses over time, underscoring intricate interplay redox-related mechanisms kidney. This sheds light early effects mixtures hens’ kidneys potential stress.

Language: Английский

Citations

1
Chronic oral exposure to low-concentration fumonisin B2 significantly exacerbates the inflammatory responses of allergies in mice via inhibition of IL-10 release by regulatory T cells in gut-associated lymphoid tissue DOI

Mana Ando,

H Yamaguchi,

Ai Morimoto

et al.

Archives of Toxicology, Journal Year: 2023, Volume and Issue: 97(10), P. 2707 - 2719

Published: Aug. 17, 2023

Language: Английский

Citations

3
Multi-Fusarium mycotoxin exposure activates Nrf2 and Ahr pathway in the liver of laying hens DOI Creative Commons
Benjámin Kövesi, Szabina Kulcsár, Zsolt Ancsin

et al.

Toxicology Letters, Journal Year: 2023, Volume and Issue: 391, P. 55 - 61

Published: Dec. 12, 2023

This study investigates gene expression changes in laying hens exposed to trichothecene mycotoxins, known induce oxidative stress and affect xenobiotic transformation antioxidants. A 3-day feeding trial tested low high doses of T-2/HT-2 toxin, DON/3-AcDON/15-AcDON, FB1 hen feed. Results showed increased AHR, AHRR, HSP90, CYP1A2 genes on days 2 3, suggesting a response mycotoxin exposure. High down-regulated CYP1A2, AHRR day 1. KEAP1 decreased 1 but dose-dependently 3. NRF2 was up-regulated by 1, then Antioxidant-related (GPX3, GPX4, GSS, GSR) dose-dependent responses. Low GPX3 GPX4 throughout, while 3 GSS indicate that toxic metabolites formed phase I biotransformation rapidly ROS formation at through the AHR/Hsp70/CYP1A2 pathway level, levels, ROS-induced manifests later. Study simultaneous activation redox-sensitive pathways: aryl hydrocarbon receptor (Ahr) nuclear factor erythroid-derived 2-like (Nrf2) multi-mycotoxin

Language: Английский

Citations

2
Co-Occurrence of Fusarium and Alternaria Metabolites in Brewing Barley Monitored during Two Consecutive Years (2019–2020) DOI Creative Commons

Kristina Habschied,

Krešimir Mastanjević, Jurislav Babić

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(9), P. 1156 - 1156

Published: Sept. 14, 2024

Known mycotoxins have been investigated for years. They included in legislation and are meticulously controlled most cereals, cereal-related products, raw materials of animal origin. However, there still that need to be addressed by regulations subsequently not monitored but can occur relatively high concentrations. This research aimed assess the occurrence common Fusarium hulled barley. Samples barley were treated field with two protective treatments, alongside a control sample sans treatment. Furthermore, we Alternaria chosen samples. The results shown mostly determined climatic conditions (no 2020, except siccanol). Most interesting was appearance infectopyron, an toxin detected all samples 2019 majority 2020. highest concentration 536 µg/kg, while this mycotoxin reached 350 µg/kg. These findings depict further on food safety regarding mycotoxins, additional changes legislation. investigation shows fungicide application rainy years cannot efficiently suppress production. Additionally, even dry years, some involved legislation, such as infectopyron siccanol, do respond fungicides.

Language: Английский

Citations

0
High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Identifies Impairment of Metabolism by T-2 Toxin, in Relation to Toxicity, in Zebrafish Embryo Model DOI Creative Commons

Ariel Lawson,

Mark Annunziato,

Narmin Bashirova

et al.

Toxins, Journal Year: 2024, Volume and Issue: 16(10), P. 424 - 424

Published: Oct. 1, 2024

Among the widespread trichothecene mycotoxins, T-2 toxin is considered most toxic congener. In present study, we utilized high-resolution magic-angle spinning nuclear magnetic resonance (HRMAS NMR), coupled to zebrafish (Danio rerio) embryo model, as a toxicometabolomics approach elucidate cellular, molecular and biochemical pathways associated with toxicity. Aligned previous studies in exposure was lethal high parts-per-billion (ppb) range, median concentration (LC50) of 105 ppb. Exposure toxins was, furthermore, system-specific alterations production reactive oxygen species (ROS), including decreased ROS liver increased brain region, exposed embryos. Moreover, metabolic profiling based on HRMAS NMR revealed modulation numerous, interrelated metabolites, specifically those (1) phase I II detoxification, antioxidant pathways; (2) disruption phosphocholine lipids cell membranes; (3) mitochondrial energy metabolism, apparent tricarboxylic acid (TCA) cycle, electron transport chain oxidative phosphorylation, well “upstream” effects carbohydrate, i.e., glucose metabolism; (4) several compensatory catabolic pathways. Taken together, these observations enabled development an integrated, system-level model toxicity relation human animal health.

Language: Английский

Citations

0
Implicación de las Fusariotoxinas en la producción avícola. Revisión DOI Creative Commons

Gabriela Guadalupe Gómez Verduzco,

Ernesto Ávila González,

Guillermo Téllez Isaías

et al.

Revista Mexicana de Ciencias Pecuarias, Journal Year: 2024, Volume and Issue: 15(2), P. 425 - 445

Published: April 23, 2024

Las micotoxinas son metabolitos secundarios producidos por hongos de diversos géneros. Dentro las más importantes se encuentran aquellas producidas del género Fusarium sp., el cual puede dividirse en varios grupos para su estudio que grupo los tricotecenos (y toxina T-2), fumonisinas, principalmente fumonisina B1 (B1, B2, B3, B4, A1 Y A2) y la zearalenona efectos estrogénicos. Aunque fusariotoxinas causan similares debido a comparten mismo mecanismo acción; mediante alteración síntesis proteínas aves intoxicadas, es importante mencionar incidencia, así como características entre cada una ellas. Es esto apartado describen mencionado.

Citations

0