Thermo-Alkali-Tolerant Recombinant Laccase from Bacillus swezeyi and Its Degradation Potential against Zearalenone and Aflatoxin B₁

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(23), P. 13371 - 13381

Published: May 29, 2024

The enzymatic biodegradation of mycotoxins in food and feed has attracted the most interest recent years. In this paper, laccase gene from Bacillus swezeyi was cloned expressed Escherichia coli BL 21(D3). sequence analysis indicated that consisted 1533 bp. purified B. analyzed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis −12% with an estimated molecular weight 56.7 kDa. enzyme is thermo-alkali-tolerant, displaying optimal degradation zearalenone (ZEN) aflatoxin B1 (AFB1) at pH 8 9, incubation temperatures 55 50 °C, respectively, within 24 h. potentials μg against ZEN (5.0 μg/mL) AFB1 (2.5 were 99.60 96.73%, To best our knowledge, first study revealing recombinant production swezeyi, its biochemical properties, potential use vitro vivo.

Language: Английский

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Biological Strategies for Aflatoxin Mitigation in Food and Feed Chains in a Changing World

IntechOpen eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

Aflatoxins (AFs) are secondary metabolites produced by Aspergillus section Flavi, mainly flavus and parasiticus. These species may infect food feed along entire agri-food chains, synthesizing the toxin when environmental conditions favorable. The scenario of climate change that world will have to face in next decades is expected increase levels AF contamination cereals, nuts, spices, legumes, among other important crops around world. This situation a great impact on human animal health, as well economic losses due rejection contaminated products during commercialization. Several mitigation strategies been implemented counteract this problem. Some them intended prevent synthesis, while others designed decontaminate pre-formed toxin. In addition, several pre- post-harvest shown effectivity. Besides physical chemical tools, biological control based different microorganisms use enzymes proposed promissory approaches mitigate risk contamination. chapter, detailed information about two developments presented: biocontrol prevention non-toxigenic A. strain fungal laccases AFs. integration these tools enhance quality feed, contributing safety chains.

Language: Английский

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A cost-saving, safe, and highly efficient natural mediator for laccase application on aflatoxin detoxification

Food Chemistry, Journal Year: 2024, Volume and Issue: 455, P. 139862 - 139862

Published: May 29, 2024

Laccase mediators possess advantage of oxidizing substrates with high redox potentials, such as aflatoxin B1 (AFB1). High costs chemically synthesized limit laccase industrial application. In this study, thin stillage extract (TSE), a byproduct corn-based ethanol fermentation was investigated the potential natural mediator laccases. Ferulic acid, p-coumaric and vanillic acid were identified predominant phenolic compounds TSE. With assistance 0.05 mM TSE, AFB1 degradation activity novel Glac1 increased by 17 times. The promoting efficiency TSE similar to ferulic but superior 1.2- 1.3-fold increases, respectively. After Glac1-TSE treatment, two oxidation products identified. Ames test showed lost mutagenicity. Meanwhile, also 1.3–3.0 times effect on in cereal flours. Collectively, safe highly efficient obtained for detoxification.

Language: Английский

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Fungal Laccases and Fumonisin Decontamination in Co-Products of Bioethanol from Maize

Toxins, Journal Year: 2024, Volume and Issue: 16(8), P. 350 - 350

Published: Aug. 10, 2024

Maize (

Language: Английский

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