Analytica Chimica Acta, Journal Year: 2022, Volume and Issue: 1225, P. 340250 - 340250
Published: Aug. 12, 2022
Language: Английский
Protein Expression and Purification, Journal Year: 2025, Volume and Issue: unknown, P. 106681 - 106681
Published: Feb. 1, 2025
Language: Английский
Citations
0
Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 45 - 82
Published: Jan. 1, 2025
Language: Английский
Citations
0
Published: April 15, 2025
Nanobodies, single-domain antibodies derived from camelid heavy-chain antibodies, are known for their high affinity, stability, and small size, which make them useful in biological research therapeutic applications. However, traditional nanobody generation methods rely on immunization, can be costly time- consuming, restricting practical feasibility. In this study, we present a phage- displayed synthetic library discovery. To validate approach, screened nanobodies targeting various Drosophila secreted proteins. The identified were suitable applications such as immunostaining immunoblotting, supporting the phage-displayed versatile platform development. address challenge of limited accessibility to high-quality libraries, will openly available non-profit use.
Language: Английский
Citations
0
Published: April 15, 2025
Nanobodies, single-domain antibodies derived from camelid heavy-chain antibodies, are known for their high affinity, stability, and small size, which make them useful in biological research therapeutic applications. However, traditional nanobody generation methods rely on immunization, can be costly time- consuming, restricting practical feasibility. In this study, we present a phage- displayed synthetic library discovery. To validate approach, screened nanobodies targeting various Drosophila secreted proteins. The identified were suitable applications such as immunostaining immunoblotting, supporting the phage-displayed versatile platform development. address challenge of limited accessibility to high-quality libraries, will openly available non-profit use.
Language: Английский
Citations
0
mAbs, Journal Year: 2024, Volume and Issue: 16(1)
Published: April 18, 2024
There is no doubt that today's life sciences would look very different without the availability of millions research antibody products. Nevertheless, use reagents are poorly characterized has led to publication false or misleading results. The laboratory animals produce antibodies also been criticized. Surprisingly, both problems can be addressed with same technology. This review charts maze formats and various methods for production their interconnections, ultimately concluding sequence-defined recombinant offer a clear path improved quality experimental data reduced animals.
Language: Английский
Citations
3
Avicenna Journal of Medical Biotechnology, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 25, 2025
Background: Since the initial outbreak, SARS-CoV-2 virus has continued to circulate and mutate, resulting in emergence of new viral sublineages. Due lack effective protection therapeutic measures against these variants, is able further evolve diversify. This study aimed screen a phage antibody library identify monoclonal antibodies single-chain variable fragment (scFv) format that target Receptor Binding Domain (RBD) different strains. The newly discovered scFv potential for use as diagnostic or option SARS-CoV-2. Methods: RBD protein was produced, purified, used an antigen during bio-panning. Six rounds panning enriched RBD-specific phages binding affinity binders were monitored by polyclonal ELISA. Subsequently, ELISA employed specific binders. After sequence confirmation, reactivity isolated anti-RBD evaluated. Additionally, bioinformatics tools determined interaction between selected Results: analysis demonstrated expressed retains its structural integrity effectively interacts with present sera COVID-19 patients. Through screening display library, strong-binding discovered, which can neutralize novel variants. Conclusion: findings this have led discovery neutralizes strains, offering immense research therapy purposes.
Language: Английский
Citations
0
Briefings in Bioinformatics, Journal Year: 2024, Volume and Issue: 25(6)
Published: Sept. 23, 2024
Abstract High affinity is crucial for the efficacy and specificity of antibody. Due to involving high-throughput screens, biological experiments antibody maturation are time-consuming have a low success rate. Precise computational-assisted design promises accelerate this process, but there still lack effective computational methods capable pinpointing beneficial mutations within complementarity-determining region (CDR) antibodies. Moreover, random often lead challenges in expression immunogenicity. In study, enhance human against avian influenza virus, CDR library was constructed evolutionary information acquired through sequence alignment restrict mutation positions types. Concurrently, statistical potential methodology developed based on amino acid interactions between antibodies antigens calculate affinity-enhanced antibodies, which were further subjected molecular dynamics simulations. Subsequently, experimental validation confirmed that point enhancing 2.5-fold obtained from 10 designs, resulting 2 nM. A predictive model antibody–antigen binding interface also developed, achieving an Area Under Curve (AUC) 0.83 precision 0.89 test set. Lastly, novel approach combinations affinity-enhancing iterative optimization scheme similar Monte Carlo method proposed. This study presents rapidly accurately affinity, addressing issues related
Language: Английский
Citations
3
Viruses, Journal Year: 2021, Volume and Issue: 13(6), P. 1120 - 1120
Published: June 10, 2021
The latest coronavirus disease outbreak, COVID-19, has brought attention to viral infections which have posed serious health threats humankind throughout history. rapid global spread of COVID-19 is attributed the increased human mobility today's world, yet threat public expected increase continuously in part due increasing human-animal interface. Development antiviral agents crucial combat both existing and novel infections. Recently, there a growing interest peptide/protein-based drug molecules. Antibodies are becoming especially predominant market. Indeed, remarkably short period, four antibody therapeutics were authorized for emergency use treatment US, Russia, India as November 2020. Phage display been one most widely used screening methods peptide/antibody discovery. Several phage display-derived biologics already market, expiration intellectual property rights phage-display discovery platforms suggests an increment drugs near future. This review summarizes common libraries discovery, highlights approaches employed enhance potency selected peptides/antibody fragments, finally provides discussion about present status developed antivirals clinic.
Language: Английский
Citations
18
Biomaterials Science, Journal Year: 2022, Volume and Issue: 10(19), P. 5459 - 5471
Published: Jan. 1, 2022
Modified nanoparticles for targeting atherosclerotic overexpressed receptors.
Language: Английский
Citations
13
Viruses, Journal Year: 2023, Volume and Issue: 15(11), P. 2177 - 2177
Published: Oct. 30, 2023
Antibody-based passive immunotherapy has been used effectively in the treatment and prophylaxis of infectious diseases. Outbreaks emerging viral infections from arthropod-borne viruses (arboviruses) represent a global public health problem due to their rapid spread, urging measures infected individuals combat them. Preparedness advances developing antivirals relevant epidemiological studies protect us damage losses. Immunotherapy based on monoclonal antibodies (mAbs) shown be very specific combating diseases various other illnesses. Recent mAb discovery techniques have allowed development approval wide number therapeutic mAbs. This review focuses technological approaches available select neutralizing mAbs for arbovirus next-generation strategies obtain highly effective potent The characteristics developed as prophylactic antiviral agents dengue, Zika, chikungunya, West Nile tick-borne encephalitis virus are presented, well protective effect demonstrated animal model studies.
Language: Английский
Citations
7