Genomic Characteristics of Feline Anelloviruses Isolated from Domestic Cats in Shanghai, China DOI Creative Commons
Jun Gao, Chengqian Liu,

Jianzhong Yi

et al.

Veterinary Sciences, Journal Year: 2023, Volume and Issue: 10(7), P. 444 - 444

Published: July 7, 2023

Viral metagenomics techniques allow the high-throughput discovery of possible pathogens carried by companion animals from their feces and other excreta. In this study, viral 22 groups fecal samples domestic cats revealed a high prevalence feline anelloviruses (FcTTV) infection in Shanghai, China. Serum 30 cat individuals were further detected polymerase chain reaction, an average positive rate 36.67% (11/30) FcTTV was found. Next, full-length sequences five Shanghai variants obtained submitted to GenBank with access numbers OP186140 OP186144. Phylogenetic analysis indicates that have relatively consistent genomic characteristics, two Zhejiang 2019 one variant Czech Republic 2010. The recombination event showed (OP186141_SH-02) had primary parental sequence derived (KM229764) 2010, while secondary OP186140_SH-01. results is prevalent use rapidly identify some infecting viruses whose hosts lack clinical features would be effective approach.

Language: Английский

An Updated Review on SARS-CoV-2 Infection in Animals DOI Creative Commons
Shujuan Cui, Yimeng Liu, Jiachen Zhao

et al.

Viruses, Journal Year: 2022, Volume and Issue: 14(7), P. 1527 - 1527

Published: July 13, 2022

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has lasted for two years and caused millions of infections deaths in humans. Although the origin SARS-CoV-2 infection humans remains unknown, animals been frequently reported varieties all over world. Both experimental natural different animal species provide useful information on viral host range pathogenicity. As continues to evolve, will be expanding. In this review, we summarized testing as well strains transmission animals. Current data showed that at least 18 tested positive SARS-CoV-2. These belong pet, captive, farmed, wild Fifteen eighteen were known Delta variant ten infected with types variants. Human-to-animal, animal-to-animal, animal-to-human events suggested outbreaks involved Continued testing, immunization, surveillance are warranted.

Language: Английский

Citations

64
Multiple Introductions of SARS-CoV-2 Alpha and Delta Variants into White-Tailed Deer in Pennsylvania DOI Creative Commons
Andrew D. Marques, Scott Sherrill-Mix, J.K. Everett

et al.

mBio, Journal Year: 2022, Volume and Issue: 13(5)

Published: Aug. 24, 2022

Coronaviruses have been documented to replicate in numerous species of vertebrates, and multiple spillovers coronaviruses from animals into humans founded human epidemics. The COVID-19 epidemic likely derived a spillover SARS-CoV-2 bats humans, possibly via an intermediate host.

Language: Английский

Citations

59
Evolution of SARS-CoV-2 in white-tailed deer in Pennsylvania 2021–2024 DOI Creative Commons
Andrew D. Marques,

Matthew Hogenauer,

Natalie Bauer

et al.

PLoS Pathogens, Journal Year: 2025, Volume and Issue: 21(1), P. e1012883 - e1012883

Published: Jan. 24, 2025

SARS-CoV-2 continues to transmit and evolve in humans animals. White-tailed deer ( Odocoileus virginianus ) have been previously identified as a zoonotic reservoir for with high rates of infection probable spillback into humans. Here we report sampling 1,127 white-tailed (WTD) Pennsylvania, genomic analysis viral dynamics spanning 1,017 days between April 2021 January 2024. To assess load genotypes, RNA was isolated from retropharyngeal lymph nodes analyzed using RT-qPCR whole genome sequencing. Samples showed 14.64% positivity rate by RT-qPCR. Analysis no association prevalence age, sex, or diagnosis Chronic Wasting Disease. From the 165 positive WTD, recovered 25 sequences an additional 17 spike-targeted amplicon sequences. The variants included Alpha, 11 Delta, 14 Omicron. Alpha largely stopped circulating around September 2021, but persisted WTD recently March 2023. Phylodynamic pooled data Pennsylvania documents at least 12 spillovers including recent series Omicron spillovers. Prevalence higher regions crop coverage rather than forest, suggesting proximity seasonality increased winter spring. Multiple examples recurrent mutations were associated transmissions, WTD-specific evolutionary pressures. These document ongoing infections deer, onward transmission remarkable new

Language: Английский

Citations

1
SARS‐CoV‐2 and natural infection in animals DOI

Xinyu Qiu,

Yi Liu, Ailong Sha

et al.

Journal of Medical Virology, Journal Year: 2022, Volume and Issue: 95(1)

Published: Sept. 19, 2022

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is the causative agent of novel coronavirus disease (COVID-19) pandemic, which has caused serious challenges for public health systems worldwide. Due to close relationship between animals and humans, confirmed transmission from humans numerous animal species been reported. Understanding cross-species SARS-CoV-2 infection dynamics in different crucial control COVID-19 protect health. In this review, possible origins naturally susceptible are discussed. Furthermore, review categorizes by families, so as better understand animals.

Language: Английский

Citations

31
Detection and Molecular Characterization of the SARS-CoV-2 Delta Variant and the Specific Immune Response in Companion Animals in Switzerland DOI Creative Commons
Evelyn Kuhlmeier, Tatjana Chan, Cecilia Valenzuela Agüí

et al.

Viruses, Journal Year: 2023, Volume and Issue: 15(1), P. 245 - 245

Published: Jan. 15, 2023

In human beings, there are five reported variants of concern severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2). However, in contrast to descriptions infections animals with specific still rare. The aim this study is systematically investigate SARS-CoV-2 companion close contact SARS-CoV-2-positive owners (“COVID-19 households”) a focus on the Delta variant. Samples, obtained from and their were analyzed using real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) next-generation sequencing (NGS). Animals also tested for antibodies neutralizing activity against SARS-CoV-2. Eleven cats three dogs nine COVID-19-positive households RT-qPCR and/or serologically positive For seven animals, genetic sequence could be determined. infected by one pangolin lineages B.1.617.2, AY.4, AY.43 AY.129 between zero single-nucleotide polymorphisms (SNPs) detected viral genomes owners, indicating within-household transmission animal owner multi-pet animals. NGS data identified SNPs that occur at higher frequency sequences than humans, as well SNPs, which exclusively found investigated current not owners. conclusion, our first describe variant Switzerland provides first-ever description Delta-variant AY.4 Our results reinforce need One Health approach monitoring

Language: Английский

Citations

14
SARS-CoV-2 Affects Both Humans and Animals: What Is the Potential Transmission Risk? A Literature Review DOI Creative Commons
Antonio Santaniello, Giuseppe Perruolo,

Serena Cristiano

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(2), P. 514 - 514

Published: Feb. 17, 2023

In March 2020, the World Health Organization Department declared coronavirus (COVID-19) outbreak a global pandemic, as consequence of its rapid spread on all continents. The COVID-19 pandemic has been not only health emergency but also serious general problem fear contagion and severe restrictions put economic social activity hold in many countries. Considering close link between human animal health, might infect wild companion animals, spawn dangerous viral mutants that could jump back pose an ulterior threat to us. purpose this review is provide overview with particular focus clinical manifestations humans different diagnosis methods, potential transmission risks, their direct impact human–animal relationship.

Language: Английский

Citations

13
Multiplex One-Step RT-qPCR Assays for Simultaneous Detection of SARS-CoV-2 and Other Enteric Viruses of Dogs and Cats DOI Creative Commons
Côme Thieulent, Mariano Carossino,

Laura Peak

et al.

Viruses, Journal Year: 2023, Volume and Issue: 15(9), P. 1890 - 1890

Published: Sept. 7, 2023

The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) was transmitted from humans to dogs and cats (reverse zoonosis) during the COVID-19 pandemic. SARS-CoV-2 has been detected in fecal samples of infected cats, indicating potential fecal-oral transmission, environmental contamination, zoonotic transmission (i.e., spillback). Additionally, gastrointestinal viral infections are prevalent cats. In this study, we developed validated a panel multiplex one-step reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays for simultaneous detection common canine enteric viruses: Canine Enteric Assay_1 (CEA_1) adenovirus-1, coronavirus, distemper virus, parvovirus, CEA_2 rotavirus A (RVA), SARS-CoV-2); or feline viruses (Feline (FEA_1) panleukopenia RVA, SARS-CoV-2). All demonstrated high analytical sensitivity, detecting as few 5-35 genome copies/µL format. repeatability reproducibility were excellent, with coefficient variation <4%. Among 58 clinical tested, 34.5% positive at least one these viruses, two collected dog cat, respectively. conclusion, newly RT-qPCR allow rapid diagnosis infections, including SARS-CoV-2,

Language: Английский

Citations

11
Seroprevalence of SARS-CoV-2 in Client-Owned Cats from Portugal DOI Creative Commons

Andreia Oliveira,

Maria A. Pereira, Teresa Letra Mateus

et al.

Veterinary Sciences, Journal Year: 2022, Volume and Issue: 9(7), P. 363 - 363

Published: July 16, 2022

The close contact between humans and domestic cats raises concerns about the potential risks of SARS-CoV-2 transmission. Thus, this study aims to investigate anti-SARS-CoV-2 seroprevalence in client-owned from Portugal evaluate infection risk that maintain with human COVID-19 cases. A total 176 cats, belonging 94 households, were sampled. Cat owners answered an online questionnaire, screened for antibodies against using a commercial ELISA. Twenty (21.3%) households reported at least one confirmed case. Forty (22.7%) belonged COVID-19-positive 136 (77.3%) COVID-19-negative household. seroprevalences -negative 5.0% (2/40) 0.7% (1/136). two SARS-CoV-2-seropositive had indoor lifestyle, their stated they maintained frequent them, even after being diagnosed COVID-19, pointing towards human-to-cat cat household mixed indoor/outdoor lifestyle chronic diseases. Owners three did not notice clinical signs or behavior changes. This highlights low transmission members context human-animal contact.

Language: Английский

Citations

12
Infection Dynamics, Pathogenesis, and Immunity to SARS-CoV-2 in Naturally Susceptible Animal Species DOI Open Access
Mohammed Nooruzzaman, Diego G. Diel

The Journal of Immunology, Journal Year: 2023, Volume and Issue: 211(8), P. 1195 - 1201

Published: Oct. 2, 2023

SARS-CoV-2, the causative agent of COVID-19 pandemic, presents a broad host range. Domestic cats and white-tailed deer (WTD) are particularly susceptible to SARS-CoV-2 with multiple variant strains being associated infections in these species. The virus replicates upper respiratory tract lymphoid tissues, it is shed through oral nasal secretions, which leads efficient transmission contact animals. Robust cell-mediated humoral immune responses induced upon infection domestic cats, curb progression clinical disease control infection. In WTD, high levels neutralizing Abs detected early this review, current understanding dynamics, pathogenesis, animals, special focus on naturally felids discussed.

Language: Английский

Citations

7
Aptamer-based diagnostic and therapeutic approaches for animal viruses: A review DOI

Zhuoer Chen,

Qi Sun, Ying Yang

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 257, P. 128677 - 128677

Published: Dec. 10, 2023

Language: Английский

Citations

6