Bacteriophage
(phage)
therapy
has
been
proposed
as
a
means
to
combat
drug-resistant
bacterial
pathogens.
Infection
by
phage
can
select
for
mutations
in
populations
that
confer
resistance
against
infection.
However,
yield
evolutionary
trade-offs
of
biomedical
use.
Here
we
report
the
discovery
staphylococcal
phages
cause
different
strains
methicillin-resistant
Staphylococcus
aureus
(MRSA)
become
sensitized
β-lactams,
class
antibiotics
which
MRSA
is
typically
highly
resistant.
cells
survive
infection
these
display
significant
reductions
minimal
inhibitory
concentration
β-lactams
compared
uninfected
bacteria.
Phage-treated
further
exhibited
attenuated
virulence
phenotypes
form
reduced
hemolysis
and
clumping.
Sequencing
analysis
revealed
evolved
unique
genetic
profiles
during
These
results
suggest
complex
trajectories
predation
open
up
new
possibilities
reduce
drug
infections.
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Nov. 26, 2024
In
the
context
of
antimicrobial
therapy
crisis,
significance
studying
and
implementing
alternative
treatment
methods,
particularly
phage
therapy,
is
increasingly
evident.
This
study
aimed
to
investigate
resistance
clinical
Staphylococcus
aureus
ST239
strains
Herelleviridae
phages
through
comparative
genomics,
transcriptomics,
proteomics.
Analysis
resistant
sensitive
S.
showed
that
form
a
separate
cluster
on
phylogenetic
tree,
suggesting
unique
genetic
traits
underlying
their
resistance.
Further
in-depth
analysis
SA191
strain
infected
with
phage,
compared
an
uninfected
control,
unveiled
significant
changes
in
transcription
462
genes
(271↑
191↓)
at
5
min
504
(276↑
228↓)
30
post-infection.
Proteomic
identified
184
differentially
abundant
proteins
(41↑
143↓)
min.
Functional
highlighted
glycolysis,
tricarboxylic
acid
cycle,
transport
systems;
notable,
were
also
observed
prophage
genes.
Despite
metabolic
shifts,
classical
mechanisms
related
teichoic
synthesis,
restriction-modification,
toxin-antitoxin
systems
not
identified,
existence
other
mechanism.
Our
contributes
elucidation
against
phages,
highlighting
intricate
nature
bacterial
defense
mechanisms.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Aug. 19, 2024
Abstract
Bacteriophage
(phage)
therapy
has
been
proposed
as
a
means
to
combat
drug-resistant
bacterial
pathogens.
Infection
by
phage
can
select
for
mutations
in
populations
that
confer
resistance
against
infection.
However,
yield
evolutionary
trade-offs
of
biomedical
use.
Here
we
report
the
discovery
staphylococcal
phages
cause
different
strains
methicillin-resistant
Staphylococcus
aureus
(MRSA)
become
sensitized
β-lactams,
class
antibiotics
which
MRSA
is
typically
highly
resistant.
cells
survive
infection
these
display
significant
reductions
minimal
inhibitory
concentration
β-lactams
compared
uninfected
bacteria.
Phage-treated
further
exhibited
attenuated
virulence
phenotypes
form
reduced
hemolysis
and
clumping.
Sequencing
analysis
revealed
evolved
unique
genetic
profiles
during
These
results
suggest
complex
trajectories
predation
open
up
new
possibilities
reduce
drug
infections.
Antibiot Khimioter = Antibiotics and Chemotherapy,
Journal Year:
2024,
Volume and Issue:
69(5-6), P. 63 - 71
Published: Oct. 3, 2024
The
globalization
of
the
problem
formation
bacterial
strains
poly-
and
pan-resistant
to
known
antimicrobial
drugs
creates
high
risks
in
healthcare
sector.
threat
a
return
«pre-antibiotic»
era
dictates
need
search
for
alternative
forms
antibacterial
therapy.
Phage
therapy,
based
on
use
natural,
widely
distributed
environment,
accessible
agent,
is
again
becoming
relevant.
article
highlights
mechanisms
antiviral
protection
prokaryotes
at
various
stages
interaction
between
virus
target
cell.
Revealing
secrets
confrontation
bacteria
viruses,
their
co-evolution
necessary
increase
effectiveness
phage
therapy
develop
modern
means
overcoming
resistance
agents.
Bacteriophage
(phage)
therapy
has
been
proposed
as
a
means
to
combat
drug-resistant
bacterial
pathogens.
Infection
by
phage
can
select
for
mutations
in
populations
that
confer
resistance
against
infection.
However,
yield
evolutionary
trade-offs
of
biomedical
use.
Here
we
report
the
discovery
staphylococcal
phages
cause
different
strains
methicillin-resistant
Staphylococcus
aureus
(MRSA)
become
sensitized
β-lactams,
class
antibiotics
which
MRSA
is
typically
highly
resistant.
cells
survive
infection
these
display
significant
reductions
minimal
inhibitory
concentration
β-lactams
compared
uninfected
bacteria.
Phage-treated
further
exhibited
attenuated
virulence
phenotypes
form
reduced
hemolysis
and
clumping.
Sequencing
analysis
revealed
evolved
unique
genetic
profiles
during
These
results
suggest
complex
trajectories
predation
open
up
new
possibilities
reduce
drug
infections.
Bacteriophage
(phage)
therapy
has
been
proposed
as
a
means
to
combat
drug-resistant
bacterial
pathogens.
Infection
by
phage
can
select
for
mutations
in
populations
that
confer
resistance
against
infection.
However,
yield
evolutionary
trade-offs
of
biomedical
use.
Here
we
report
the
discovery
staphylococcal
phages
cause
different
strains
methicillin-resistant
Staphylococcus
aureus
(MRSA)
become
sensitized
β-lactams,
class
antibiotics
which
MRSA
is
typically
highly
resistant.
cells
survive
infection
these
display
significant
reductions
minimal
inhibitory
concentration
β-lactams
compared
uninfected
bacteria.
Phage-treated
further
exhibited
attenuated
virulence
phenotypes
form
reduced
hemolysis
and
clumping.
Sequencing
analysis
revealed
evolved
unique
genetic
profiles
during
These
results
suggest
complex
trajectories
predation
open
up
new
possibilities
reduce
drug
infections.
