SARS-CoV-2 co-detection with influenza and human respiratory syncytial virus in Ethiopia: Findings from the severe acute respiratory illness (SARI) and influenza-like illness (ILI) sentinel surveillance, January 01, 2021, to June 30, 2022 DOI Creative Commons

Wolde Shure,

Adamu Tayachew,

Tsegaye Berkessa

et al.

PLOS Global Public Health, Journal Year: 2024, Volume and Issue: 4(4), P. e0003093 - e0003093

Published: April 18, 2024

SARS-CoV-2 co-infection with the influenza virus or human respiratory syncytial (RSV) may complicate its progress and clinical outcomes. However, data on co-detection of other viruses are limited in Ethiopia parts Africa to inform evidence-based response decision-making. We analyzed 4,989 patients' captured from national severe acute illness (SARI) influenza-like (ILI) sentinel surveillance sites over 18 months period January 01, 2021, June 30, 2022. Laboratory specimens were collected patients tested for viral pathogens by real-time, reverse transcription polymerase chain reaction (RT-PCR) at center. The median age was 14 years (IQR: 1-35 years), a slight preponderance them being 15 less than 50 years. detected among 459 (9.2%, 95% CI: 8.4-10.0) patients, 64 (1.3%, 1.0-1.6) co-detected either Influenza (54.7%) RSV (32.8%) 12.5% both viruses. A substantial proportion identified group multivariable analysis found that odds higher individuals category 20 39 as compared those old (AOR: 1.98, 95%CI:1.15-3.42) while lower cases regions country Addis Ababa (AOR:0.16 95%CI:0.07-0.34). Although minimal, findings this study underscore it is critical continuously monitor co-infections reduce transmission improve patient outcomes, particularly youth ILI.

Language: Английский

Development of a Melting-Curve-Based Multiplex Real-Time PCR Assay for the Simultaneous Detection of Viruses Causing Respiratory Infection DOI Creative Commons
Eliandro Reis Tavares,

Thiago Ferreira de Lima,

Guilherme Bartolomeu-Gonçalves

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(11), P. 2692 - 2692

Published: Nov. 2, 2023

The prompt and accurate identification of the etiological agents viral respiratory infections is a critical measure in mitigating outbreaks. In this study, we developed clinically evaluated novel melting-curve-based multiplex real-time PCR (M-m-qPCR) assay targeting RNA-dependent RNA polymerase (RdRp) nucleocapsid phosphoprotein N SARS-CoV-2, Matrix protein 2 Influenza A virus, RdRp domain L from Human Respiratory Syncytial Virus, polyprotein Rhinovirus B genes. analytical performance M-m-qPCR underwent assessment using silico analysis panel reference clinical strains, encompassing viral, bacterial, fungal pathogens, exhibiting 100% specificity. Moreover, showed detection limit 10 copies per reaction for all targeted pathogens positive controls. To validate its applicability, was further tested simulated nasal fluid spiked with viruses mentioned above, followed by validation on nasopharyngeal swabs collected 811 individuals. Among them, 13.4% (109/811) 1.1% (9/811) A. Notably, these results concordance those obtained commercial kit. Therefore, exhibits great potential routine screening pathogens.

Language: Английский

Citations

4
Co-circulation and co-infection of COVID-19 and influenza in China: challenges and implications DOI Creative Commons
Qingchun Pan, Zhenghao Tang, Yong-Sheng Yu

et al.

Frontiers in Public Health, Journal Year: 2023, Volume and Issue: 11

Published: Dec. 8, 2023

OPINION article Front. Public Health, 08 December 2023Sec. Infectious Diseases: Epidemiology and Prevention Volume 11 - 2023 | https://doi.org/10.3389/fpubh.2023.1295877

Language: Английский

Citations

4
Viral coinfection in hospitalized patients during the COVID-19 pandemic in Southern Brazil: a retrospective cohort study DOI Creative Commons
Jaqueline Rhoden,

Andressa Taíz Hoffmann,

Janaína Franciele Stein

et al.

Respiratory Research, Journal Year: 2024, Volume and Issue: 25(1)

Published: Feb. 5, 2024

Abstract Purpose Since the worldwide spread of SARS-CoV-2, different strategies have been followed to combat pandemic and limit virus transmission. In meantime, other respiratory viruses continued circulate, though at decreased rates. Methods This study was conducted between June July 2022, in a hospital metropolitan region Rio Grande do Sul state, southernmost state Brazil. The 337 hospitalized patients included those with symptoms without delimitation age. Reverse transcription-quantitative real-time polymerase chain reaction detected 15 confirmed coinfections samples. Different statistical tests were applied evaluate association associations clinical characteristics coinfection. Results Sampling corresponds selected 330 analyzed. principal outcome found discharge 309 (94%) cases, while 21 (6%) resulted death. viral agents related Human rhinovirus, enterovirus, Respiratory syncytial virus. most frequent agent 60 (18%) infections, by 51 (15%) cases B 44 (13%) rhinovirus 1. Coinfection mainly observed children, adults elderly more affected single infection. Analyzing COVID-19 vaccination, 175 (53%) unvaccinated remainder had least one dose vaccine. Conclusions presents information update understanding circulation region. Furthermore, findings clarify behavior infections possible patients, considering ages profiles. addition, this knowledge can help monitor population’s manifestations prevent future outbreaks viruses.

Language: Английский

Citations

1
Coxsackievirus A7 and Enterovirus A71 Significally Reduce SARS-CoV-2 Infection in Cell and Animal Models DOI Open Access
V. A. Svyatchenko,

Stanislav S. Legostaev,

Roman Y. Lutkovskiy

et al.

Published: March 14, 2024

In this study, we investigated the features of co-infection with SARS-CoV-2 and nonpathogenic strain LEV8 coxsackievirus A7 or enterovirus A71 for Vero E6 cells Syrian hamsters. The investigation SARS-СoV-2 LEV-8 EV-A71 in cell model was shown that a competitive inhibitory effect these viruses were especially significant against SARS-CoV-2. Pre-infection enteroviruses animals caused more than 100-fold decrease levels virus replication respiratory tracts rapid clearance lower tract from infectious Co-infection also reduced severity clinical manifestations infection animals. Additionally, histological data illustrated decreased level pathological changes by lungs. Research into chemokine/cytokine profile demonstrated studied efficiently triggered part antiviral immune response, which can be associated inhibition infection. These results demonstrate there exists strong viral interference between pathogenic humans vitro vivo.

Language: Английский

Citations

1
SARS-CoV-2 co-detection with influenza and human respiratory syncytial virus in Ethiopia: Findings from the severe acute respiratory illness (SARI) and influenza-like illness (ILI) sentinel surveillance, January 01, 2021, to June 30, 2022 DOI Creative Commons

Wolde Shure,

Adamu Tayachew,

Tsegaye Berkessa

et al.

PLOS Global Public Health, Journal Year: 2024, Volume and Issue: 4(4), P. e0003093 - e0003093

Published: April 18, 2024

SARS-CoV-2 co-infection with the influenza virus or human respiratory syncytial (RSV) may complicate its progress and clinical outcomes. However, data on co-detection of other viruses are limited in Ethiopia parts Africa to inform evidence-based response decision-making. We analyzed 4,989 patients' captured from national severe acute illness (SARI) influenza-like (ILI) sentinel surveillance sites over 18 months period January 01, 2021, June 30, 2022. Laboratory specimens were collected patients tested for viral pathogens by real-time, reverse transcription polymerase chain reaction (RT-PCR) at center. The median age was 14 years (IQR: 1-35 years), a slight preponderance them being 15 less than 50 years. detected among 459 (9.2%, 95% CI: 8.4-10.0) patients, 64 (1.3%, 1.0-1.6) co-detected either Influenza (54.7%) RSV (32.8%) 12.5% both viruses. A substantial proportion identified group multivariable analysis found that odds higher individuals category 20 39 as compared those old (AOR: 1.98, 95%CI:1.15-3.42) while lower cases regions country Addis Ababa (AOR:0.16 95%CI:0.07-0.34). Although minimal, findings this study underscore it is critical continuously monitor co-infections reduce transmission improve patient outcomes, particularly youth ILI.

Language: Английский

Citations

1