Molecular characterization of SARS-CoV-2 nucleocapsid protein

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: May 23, 2024

Corona Virus Disease 2019 (COVID-19) is a highly prevalent and potent infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Until now, the world still endeavoring to develop new ways diagnose treat COVID-19. At present, clinical prevention treatment of COVID-19 mainly targets spike protein on surface SRAS-CoV-2. However, with continuous emergence SARS-CoV-2 Variants concern (VOC), targeting therapy shows high degree limitation. The Nucleocapsid Protein (N protein) conserved in virus evolution involved key process viral infection assembly. It most expressed structural after humans has immunogenicity. Therefore, N as factor replication basic research application great potential value. This article reviews progress structure biological function protein, diagnosis drug order promote researchers’ further understanding lay theoretical foundation for possible outbreak sudden diseases future.

Language: Английский

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Revolutionizing SARS-CoV-2 omicron variant detection: Towards faster and more reliable methods

Talanta, Journal Year: 2023, Volume and Issue: 266, P. 124937 - 124937

Published: July 12, 2023

Language: Английский

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Validation of the United States Isolation Termination Criteria Using Virus Culture Results of the Omicron Variant in Japan

Journal of Infection and Chemotherapy, Journal Year: 2025, Volume and Issue: unknown, P. 102714 - 102714

Published: April 1, 2025

Language: Английский

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Recent Advances in Enzyme‐Mediated Rolling Circle Replication Technology Empowering Virus Detection

Chinese Journal of Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 11, 2025

Comprehensive Summary The prevalence and spread of viral infectious diseases pose a grave threat to global public health, particularly resulting in significant number casualties. To curb the diseases, virus testing is one efficient economical means, among which nucleic acid detection has advantage detecting infections at an early stage, even before symptoms appear. Rolling circle replication representative enzyme‐mediated isothermal amplification technology, characterized by its mild reaction conditions, no need for temperature control equipment, high efficiency. This review provides conceptual overview latest advances rolling (RCR) their applications detection, focusing on molecular design principles different application scenarios. first part briefly describes significance advantages RCR detection. second elaborates principle preparation strategy derivative technologies. third focuses various scenarios In end, we provide perspective innovation improving accuracy specificity cope with challenges that may arise future.

Language: Английский

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Pathogen Discovery in the Post-COVID Era

Pathogens, Journal Year: 2024, Volume and Issue: 13(1), P. 51 - 51

Published: Jan. 5, 2024

Pathogen discovery plays a crucial role in the fields of infectious diseases, clinical microbiology, and public health. During past four years, global response to COVID-19 pandemic highlighted importance early accurate identification novel pathogens for effective management prevention outbreaks. The post-COVID era has ushered new phase disease research, marked by accelerated advancements pathogen discovery. This review encapsulates recent innovations paradigm shifts that have reshaped landscape pandemic. Primarily, we summarize latest technology innovations, applications, causation proving strategies enable rapid both acute historical infections. We also explored significance trends approaches being employed implementation from various environmental samples. Furthermore, emphasize collaborative nature response, which led establishment networks

Language: Английский

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Rapid detection of SARS-CoV-2 RNA using a one-step fast multiplex RT-PCR coupled to lateral flow immunoassay

BMC Infectious Diseases, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 18, 2024

The COVID-19 has put emphasis on pivotal needs for diagnosis and surveillance worldwide, with the subsequent shortage of diagnostic reagents kits. Therefore, it become strategic countries to access diagnostics, expand testing capacity, develop their own capabilities alternative rapid accurate nucleic acid diagnostics that are at lower costs. Here, we propose a visual SARS-CoV-2 detection using one-step fast multiplex reverse transcription-PCR (RT-PCR) amplification coupled lateral flow immunoassay PCRD device (Abingdon Health, UK). We developed various simplex fast-PCRs screening sets primer pairs newly designed or selected from literature validated WHO targeting S, N, E, RdRp ORF1ab genes. retained primers showing specific stable assess suitability PCRD. Thus, RT-PCR amplifications were performed primers. They doubly labeled Fam Biotin Dig allow amplicons PCR Detection (PCRD) device, looking lack interaction (or dimers) test-lines in negative no RNA controls. set up all assays RNAs isolated patients' nasopharyngeal swabs. used two assays, different viral genomic regions (N E) PCRD, assay (where both differently engaged) amplicons' device. evaluated this novel 50 positive samples compared its performance results quantitative (RT-qPCR) diagnosing patients, here considered as standard tests. new achieved sensitivity 88% (44/50) specificity 98% (49/50). All patients who presented Ct values than 33 our assay. Except one patient, those above returned results. Our have brought proof principle usefulness RT- specific, sensitive, without requiring costly laboratory equipment, thus, reduced costs format prone be deployed when resources limited. This offers viable points need.

Language: Английский

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Molecular Point‐of‐Care Assay Development: Design and Considerations

Current Protocols, Journal Year: 2024, Volume and Issue: 4(6)

Published: June 1, 2024

Abstract Molecular diagnostic point‐of‐care (MDx POC) testing is gaining momentum and increasingly important for infectious disease detection monitoring, as well other areas such oncology. has traditionally required high‐complexity laboratories. Laboratory complexity determined by utilizing the Clinical Improvement Amendments of 1988 (CLIA) Categorization Criteria scorecard, seven criteria that are scored on a scale one to three. Previously, most commercially available (POC) tests use analytes technologies were not found be highly complex CLIA scoring system. However, during COVID‐19 pandemic, MDx POC became much more prominent. Utilization pandemic demonstrated applications can have outstanding advantages compared non‐molecular tests. This article introduces students, technologists, researchers, others, providing general algorithm test development. an introductory, step‐by‐step decision tree defining molecular device meeting functional requirements desired application. The technical considerations driving decision‐making include nucleic acid selection method (DNA, RNA), extraction methods, sample preparation, number targets, amplification technology, method. scope this includes neither higher‐order multiplexing, nor quantitative analysis. covers key application considerations, sensitivity, specificity, turnaround time, shipping/storage requirements. provides overall understanding best resources practices when developing assay may helpful resource readers without extensive experience those who already familiar with want increase availability at POC. © 2024 Wiley Periodicals LLC.

Language: Английский

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Solid-State Nanopore Counting of Amplicons from Recombinase Polymerase Isothermal Amplification

Sensors & Diagnostics, Journal Year: 2024, Volume and Issue: 3(10), P. 1733 - 1742

Published: Jan. 1, 2024

A nanopore-integrated reverse-transcription recombinase polymerase amplification-based assay is employed for the direct electrical detection of synthetic SARS-CoV-2 RNA amplicons.

Language: Английский

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Rapid detection of SARS-CoV-2 RNA using a one-step fast multiplex RT-PCR coupled to lateral flow immunoassay

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: July 18, 2024

Background The COVID-19 pandemics has put emphasis on pivotal needs for diagnosis and surveillance worldwide, with the subsequent shortage of diagnostic reagents kits. Therefore, it become strategic countries to be able access diagnosis, expand it, acquire its own capacity deploy diagnostics alternative rapid accurate nucleic acid tests that are at lower costs. Here, we propose a visual SARS-CoV-2 detection using one-step fast multiplex reverse transcription-PCR (RT-PCR) amplification coupled lateral flow immunoassay PCRD device (Abingdon Health, UK). Methods Various simplex fast-PCRs were developed screening sets primer pairs newly designed or selected from literature validated WHO tests, targeting S, N, E, RdRp ORF1ab genes. Primers showing specific stable retained assess their suitability PCRD. Thus, RT-PCR amplifications performed primers. They doubly labeled Fam Biotin Dig allow amplicons PCR Detection (PCRD) device, looking lack interaction primers (or dimers) test lines in negative no RNA controls. All assays set up RNAs isolated patients’ nasopharyngeal swabs. Two assays, two different viral genomic regions (N E) PCRD, used assay (where both differently engaged) amplicons’ device. This novel method was evaluated 50 positive samples performance compared results quantitative (RT-qPCR) diagnosing patients, here considered as standard methods. Results way, new showed sensitivity 88% (44/50) specificity 98% (49/50). patients who presented Ct values than 33 our assay. Except one patient, those greater results. Conclusion Our have brought proof principle usefulness RT- PCR specific, sensitive, without requiring costly laboratory equipment, thus reduced costs, format prone deployed when resources limited. appears good points need.

Language: Английский

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Recent developments in isothermal amplification technology for rapid detection of SARS-CoV-2

Analytical Methods, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

The latest achievements and prospects of LAMP RPA in the rapid detection common infectious disease pathogens such as SARS-CoV-2 is summarized.

Language: Английский

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