RSC Medicinal Chemistry, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 23, 2024
We have discovered a new class of flavonoid-based host integrated stress response (ISR) activators that exhibit potent antiviral activity against coronaviruses PEDV and PDCoV. They are host-directed antivirals (HDAs).
Language: Английский
Virology, Journal Year: 2025, Volume and Issue: 604, P. 110412 - 110412
Published: Jan. 19, 2025
Language: Английский
Citations
0
Journal of General Virology, Journal Year: 2025, Volume and Issue: 106(2)
Published: Feb. 4, 2025
Clustered regularly interspaced short palindromic repeats-associated protein 13 (CRISPR-Cas13), an RNA editing technology, has shown potential in combating viruses by degrading viral within mammalian cells. In this study, we demonstrate the effective inhibition of porcine epidemic diarrhoea virus (PEDV) replication and spread using CRISPR-Cas13. We analysed sequence similarity pseudoknot region between PEDV severe acute respiratory syndrome coronavirus 2, both belonging to Coronaviridae family, as well RNA-dependent polymerase (RdRp) gene among three different strains PED virus. Based on analysis, synthesized CRISPR RNAs (crRNAs) targeting nonpseudoknot region, each for comparison. cells treated with crRNA #3 RdRp expression decreased 95%, membrane ( M ) 89% infectious titre reduced over 95%. Additionally, nucleocapsid N levels 83 98%, respectively. The optimal concentration high antiviral efficacy without cytotoxicity was determined. Treating 1.5 µg Cas13b mRNA 0.5 resulted no while achieving 95% replication. therapeutics approach validated significantly more through a comparative study merafloxacin, drug genome. Our results indicate that plays crucial role degradation genome CRISPR-Cas13 system. Therefore, offers promising new treating infections.
Language: Английский
Citations
0
Cryobiology, Journal Year: 2025, Volume and Issue: 118, P. 105209 - 105209
Published: Feb. 11, 2025
Language: Английский
Citations
0
Journal of Virology, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 10, 2025
Porcine epidemic diarrhea, as a porcine diarrhea virus (PEDV)-induced infectious intestinal condition typified by emesis, dehydration, and anorexia, leads to death rates high 100% among suckling piglets. Given the existing commercial vaccines, it is essential study host-virus interactions formulate efficient anti-viral regimes. This concerned host factor POLM (a DNA polymerase family member) that exerts an effect against PEDV proliferation. Our results indicated expression was increased following infection regulated transcription FOXA1. In addition, our findings targeted degraded structural proteins (N, S2, M) autophagy pathway inhibit could recruit E3 ubiquitination ligase MARCH8 for N, M protein ubiquitination, which subsequently recognized p62, cargo receptor, translocation autophagic lysosome, therefore degrading preventing summary, we showed novel therapeutic target combating PEDV, i.e., using POLM-MARCH8-p62-autophagosome degrade proteins.IMPORTANCEPEDV coronavirus causes mortality in piglets, poses significant economic damage swine farming. During infection, cells may promote natural immune response suppress viral replication through variety of potential factors. this study, found upregulation FOXA1 factor) during infection. It be new replication, interacted with (an ubiquitin ligase) p62 receptor) facilitate degradation via process. Apart from elucidating previously unidentified function POLM, also provides perspective studying factors act regulators anti-PEDV pathways.
Language: Английский
Citations
0
Biomolecules, Journal Year: 2024, Volume and Issue: 14(9), P. 1116 - 1116
Published: Sept. 4, 2024
Porcine epidemic diarrhea virus (PEDV) has caused significant economic losses to the pig farming industry in various countries for a long time. Currently, there are no highly effective preventive or control measures available. Research into pathogenic mechanism of PEDV shown that it primarily causes infection by binding S protein CD13 (APN) receptor on membrane porcine intestinal epithelial cells. The S1 region contains three neutralization epitopes and multiple receptor-binding domains, which closely related viral antigenicity ad-sorption invasion. Nanobodies type single-domain antibody have been discovered recent years. They can be expressed large scale through prokaryotic expression systems, makes them cost-effective, stable, less immunogenic. This study used phage display library nanobodies against protein. After rounds selection enrichment, DNA sequence specific nanobody S1Nb1 was successfully obtained. To obtain soluble S1Nb1, its inserted vector Pcold solubility-enhancing SUMO tag added. resulting recombinant vector, Pcold-SUMO-S1Nb1, then transformed
Language: Английский
Citations
1
International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 279, P. 135375 - 135375
Published: Sept. 6, 2024
Language: Английский
Citations
0
RSC Medicinal Chemistry, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 23, 2024
We have discovered a new class of flavonoid-based host integrated stress response (ISR) activators that exhibit potent antiviral activity against coronaviruses PEDV and PDCoV. They are host-directed antivirals (HDAs).
Language: Английский
Citations
0