SPR Biosensor Based on Bilayer MoS2 for SARS-CoV-2 Sensing

Biosensors, Journal Year: 2025, Volume and Issue: 15(1), P. 21 - 21

Published: Jan. 4, 2025

The COVID-19 pandemic has highlighted the urgent need for rapid, sensitive, and reliable diagnostic tools detecting SARS-CoV-2. In this study, we developed optimized a surface plasmon resonance (SPR) biosensor incorporating advanced materials to enhance its sensitivity specificity. Key parameters, including thickness of silver layer, silicon nitride dielectric molybdenum disulfide (MoS2) layers, ssDNA recognition were systematically achieve best balance between sensitivity, resolution, attenuation. configuration, consisting 45 nm 13 2 MoS2 5 demonstrated superior performance SARS-CoV-2 in PBS solution. exhibited high at low viral concentrations, achieving 375.01°/RIU, detection accuracy 0.002, quality factor 38.34 1.0 mM concentration. Performance metrics validated sensor’s capability detection, particularly early-stage diagnostics where timely intervention is critical. Moreover, biosensor’s linear response refractive index changes confirmed potential quantitative concentration analysis. This study underlines significance integrating materials, such as nitride, SPR performance. findings establish proposed robust precise tool with applications clinical epidemiological monitoring.

Language: Английский

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In Vitro Efficacy of Antivirals and Monoclonal Antibodies against SARS-CoV-2 Omicron Lineages XBB.1.9.1, XBB.1.9.3, XBB.1.5, XBB.1.16, XBB.2.4, BQ.1.1.45, CH.1.1, and CL.1

Vaccines, Journal Year: 2023, Volume and Issue: 11(10), P. 1533 - 1533

Published: Sept. 28, 2023

The spread of COVID-19 continues, expressed by periodic wave-like increases in morbidity and mortality. reason for the is emergence novel genetic variants SARS-CoV-2. A decrease efficacy monoclonal antibodies (mAbs) has been reported, especially against Omicron subvariants. There have reports a specific antiviral drugs as result mutations genes non-structural proteins. This indicates urgent need practical healthcare to constantly monitor pathogen variability its effect on preventive therapeutic drugs. As part this study, we report results continuous monitoring Moscow using virological methods. monitoring, determined dominant identified that are most widespread, not only Moscow, but also other countries. collection viruses from more than 500 SARS-CoV-2 isolates obtained characterized. lines XBB.1.9.1, XBB.1.9.3, XBB.1.5, XBB.1.16, XBB.2.4, BQ.1.1.45, CH.1.1, CL.1, representing greatest concern, were among variants. We studied vitro mAbs Tixagevimab + Cilgavimab (Evusheld), Sotrovimab, Regdanvimab, Casirivimab Imdevimab (Ronapreve), Bebtelovimab, well Remdesivir, Molnupiravir, Nirmatrelvir, these lines. At current stage pandemic, use developed early little prospect. Specific retain their activity, further needed assess risk being reduced adjust recommendations use.

Language: Английский

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Evolution of a Distinct SARS-CoV-2 Lineage Identified during an Investigation of a Hospital Outbreak

Viruses, Journal Year: 2024, Volume and Issue: 16(3), P. 337 - 337

Published: Feb. 22, 2024

The SARS-CoV-2 virus steadily evolves, and numerous antigenically distinct variants have emerged over the past three years. Tracking evolution of would help us understand process that generates diverse predict future evolutionary trajectory SARS-CoV-2. Here, we report a unique Omicron lineage identified during an outbreak investigation occurred in residence unit healthcare system. new had four non-synonymous two synonymous mutations apart from its parental lineage. Since this was exclusively found outbreak, were able to track detailed history entire along transmission path. Furthermore, estimated rate variant analysis This sub-lineage acquired 3 12-day period, as 3.05 × 10−3 subs/site/year. study provides more insight into ever-evolving virus.

Language: Английский

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Applications of time series analysis in epidemiology: Literature review and our experience during COVID-19 pandemic

World Journal of Clinical Cases, Journal Year: 2023, Volume and Issue: 11(29), P. 6974 - 6983

Published: Oct. 13, 2023

Time series analysis is a valuable tool in epidemiology that complements the classical epidemiological models two different ways: Prediction and forecast. related to explaining past current data based on various internal external influences may or not have causative role. Forecasting an exploration of possible future values predictive ability model hypothesized and/or influences. The time approach has advantage being easier use (in cases more straightforward linear such as Auto-Regressive Integrated Moving Average). Still, it limited forecasting time, unlike Susceptible-Exposed-Infectious-Removed. Its applicability comes from its better accuracy for short-term prediction. In basic form, does assume much theoretical knowledge mechanisms spreading mutating pathogens reaction people regulatory structures (governments, companies, etc. ). Instead, estimates directly. allows testing hypotheses factors positively negatively contribute pandemic spread; be school closures, emerging variants, It can used mortality hospital risk estimation new cases, seroprevalence studies, assessing properties estimating excess relationship with pandemic.

Language: Английский

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Booster vaccines dose reduced mortality in hospitalized COVID-19 patients requiring oxygen supplementation: Evidence from the Beijing Omicron outbreak

Human Vaccines & Immunotherapeutics, Journal Year: 2024, Volume and Issue: 20(1)

Published: June 21, 2024

To assess the impact of vaccines on clinical outcomes among hospitalized COVID-19-infected patients requiring oxygen supplementation during Beijing Omicron outbreak. We conducted a retrospective cohort study at Chaoyang Hospital, Capital Medical University, from November 15, 2022, to March 31, 2023. Vaccination statuses were categorized into 3 doses, 2 and unvaccinated (0 dose). The primary outcome was 28-day all-cause mortality. Secondary included poor outcomes, intensive care unit admission, cardiovascular thromboembolism events, hospital readmission. Among patients, 117 285 received booster 503 unvaccinated. After propensity score inverse probability weighting, doses group showed significantly lower mortality compared (inverse treatment weighting-adjusted HR: 0.64, 95% CI: 0.50–0.81). No significant difference observed in between groups. differences secondary analyses when comparing or group. Subgroup analysis revealed benefits vaccination with shorter symptom duration, Charlson Comorbidity Index, without immunosuppression status. Our highlights reduction Omicron-infected who third dose vaccine. These findings underscore importance prioritizing vaccinations, especially elderly. Further research is warranted confirm extend these observations.

Language: Английский

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Neutralization of Different Variants of SARS-CoV-2 by a F(ab’)2 Preparation from Sera of Horses Immunized with the Viral Receptor Binding Domain

Published: Sept. 8, 2023

The Receptor Binding Domain (RBD) of SARS-CoV-2, the virus responsible for COVID-19 pandemic, is functional region viral Spike protein (S), which involved in cell attachment to target cells. has accumulated progressively mutations its genome, particularly RBD region, many them associated with immune evasion host neutralizing antibodies. Some lineages derived from this evolution, have been classified as Variant Interest (VOI) or Concern (VOC). capacity a F(ab’)2 preparation sera horses immunized was evaluated, by lytic plaque reduction assay, against different SARS-CoV-2 variants. A an hyperimmune serum 15 immunizations RBD, exhibited high titer antibodies ancestral-like strain (1/18,528). title observed variants tested. highest Omicron VOC (4.7 fold), followed Mu VOI (2.6), Delta (1.8 fold) and Gamma (1.5). Even if progressive evaluated observed, still significant titer, (1/7113 1/3918 respectively), strains most resistant neutralization.

Language: Английский

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Neutralization of Different Variants of SARS-CoV-2 by a F(ab′)2 Preparation from Sera of Horses Immunized with the Viral Receptor Binding Domain

Antibodies, Journal Year: 2023, Volume and Issue: 12(4), P. 80 - 80

Published: Dec. 7, 2023

The Receptor Binding Domain (RBD) of SARS-CoV-2, the virus responsible for COVID-19 pandemic, is functional region viral Spike protein (S), which involved in cell attachment to target cells. has accumulated progressively mutations its genome, particularly RBD region, many them associated with immune evasion host neutralizing antibodies. Some lineages derived from this evolution have been classified as Variant Interest (VOI) or Concern (VOC). capacity a F(ab′)2 preparation sera horses immunized was evaluated by lytic plaque reduction assay against different SARS-CoV-2 variants. A hyperimmune serum after nine immunizations exhibited high titer antibodies ancestral-like strain (1/18,528). F(ab’)2 observed variants tested compared activity strain. highest neutralization Omicron VOC (4.7-fold), followed Mu VOI (2.6), Delta (1.8-fold), and Gamma (1.5). Even if progressive observed, still (1/7113 1/3918, respectively), strains most resistant neutralization. Therefore, retained all tested.

Language: Английский

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Effectiveness and safety of COVID-19 vaccines in patients with oncological diseases: State-of-the-art

World Journal of Clinical Oncology, Journal Year: 2023, Volume and Issue: 14(9), P. 343 - 356

Published: Sept. 19, 2023

Although the coronavirus disease 2019 (COVID-19) pandemic was declared to be no longer "a public health emergency of international concern" with its wide range clinical manifestations and late complications, severe acute respiratory syndrome 2 infection proved a serious threat, especially elderly patients comorbidities. Patients oncologic diseases are vulnerable death. Indeed, oncohematological have higher risk COVID-19 impaired post-vaccination immunity. Unfortunately, cancer usually excluded from vaccine trials investigations post-vaccinal immune responses effectiveness vaccines. We aimed elucidate what extent at increased developing is their overall case fatality rate. also present current concept evidence on safety vaccines, including boosters, in oncology patients. In conclusion, despite considerably mortality patient group than general population, countries high vaccination rates demonstrated trends toward improved survival early pandemic.

Language: Английский

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COVID-19 Vaccination Reporting and Adverse Event Analysis in Taiwan

Vaccines, Journal Year: 2024, Volume and Issue: 12(6), P. 591 - 591

Published: May 29, 2024

The COVID-19 pandemic necessitated an urgent global response in vaccine deployment, achieving over 70.6% vaccination coverage with at least one dose. This study focuses on Taiwan’s administration and adverse event reporting, set against a backdrop. Using data from Vaccine Adverse Event Reporting System (VAERS) data, this investigates safety the public health implications of strategies local perspectives. proactive approach, resulting high rates, provides case for monitoring management vaccine-related events. offers insights into profiles various vaccines further explores reporting rates policy strategies. comparative analysis reveals that, while has been effective controlling virus’s spread, remains critical maintaining trust. It underscores necessity enhanced surveillance importance transparent tailored risk communication to support informed decisions. findings aim contribute dialogue safety, equitable distribution, evidence-based policy-making, development mitigation measures consideration demographics ongoing fight COVID-19.

Language: Английский

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The Molecular and Biological Patterns Underlying Sustained SARS-CoV-2 Circulation in the Human Population

Problems of Virology, Journal Year: 2024, Volume and Issue: 69(4), P. 329 - 340

Published: Sept. 26, 2024

For four years, SARS-CoV-2, the etiological agent of COVID-19, has been circulating among humans. By end second year, an absence immunologically naive individuals was observed, attributable to extensive immunization efforts and natural viral exposure. This study focuses on delineating molecular biological patterns that facilitate persistence thereby informing predictions epidemiological trajectory COVID-19 toward refining pandemic countermeasures. The aim this describe identified contribute virus in human population.

Language: Английский

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