Paromomycin targets HDAC1-mediated SUMOylation and IGF1R translocation in glioblastoma DOI Creative Commons
Min Zhong,

Yuejie Guo,

Ning Luo

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 11, 2024

Objective This study investigates the effects of Paromomycin on SUMOylation-related pathways in glioblastoma (GBM), specifically targeting HDAC1 inhibition. Methods Using TCGA and GTEx datasets, we identified genes associated with GBM prognosis. Molecular docking analysis suggested as a potential inhibitor. In vitro assays U-251MG cells were performed to assess Paromomycin’s cell viability, SUMOylation gene expression, IGF1R translocation using CCK8 assays, qRT-PCR, immunofluorescence. Results treatment led dose-dependent reduction colony formation, migration. It modulated SUMO1 expression decreased nuclear translocation, an effect reversible by inhibitor Trochostatin A (TSA), suggesting involvement SUMO1-regulated pathways. Conclusion highlights therapeutic agent for HDAC1-mediated influencing warranting further investigation its clinical application.

Language: Английский

Gradient Rotating Magnetic Fields Impairing F-Actin-Related Gene CCDC150 to Inhibit Triple-Negative Breast Cancer Metastasis by Inactivating TGF-β1/SMAD3 Signaling Pathway DOI Creative Commons
Ge Zhang,

Tongyao Yu,

Xiaoxia Chai

et al.

Research, Journal Year: 2024, Volume and Issue: 7

Published: Jan. 1, 2024

Triple-negative breast cancer (TNBC) is the most aggressive and lethal malignancy in women, with a lack of effective targeted drugs treatment techniques. Gradient rotating magnetic field (RMF) new technology used oncology physiotherapy, showing promising clinical applications due to its satisfactory biosafety abundant mechanical force stimuli it provides. However, antitumor effects underlying molecular mechanisms are not yet clear. We designed two sets gradient RMF devices for cell culture animal handling. exposure had notable impact on F-actin arrangement MDA-MB-231, BT-549, MDA-MB-468 cells, inhibiting migration invasion. A potential cytoskeleton F-actin-associated gene, CCDC150, was found be enriched TNBC tumors cells. CCDC150 negatively correlated overall survival rate patients. promoted invasion via activation transforming growth factor β1 (TGF-β1)/SMAD3 signaling pathway vitro vivo. also identified as response marked down-regulated after exposure. silencing both suppressed tumor liver metastasis. Therefore, may an treatment, emerge target therapy.

Language: Английский

Citations

4
Liquid-liquid phase separation in cell physiology and cancer biology: recent advances and therapeutic implications DOI Creative Commons
Ziyuan Huang, Zimeng Liu,

Lieqian Chen

et al.

Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 15

Published: March 31, 2025

Liquid-liquid phase separation (LLPS) is a pivotal biophysical phenomenon that plays critical role in cellular organization and has garnered significant attention the fields of molecular mechanism pathophysiology cancer. This dynamic process involves spontaneous segregation biomolecules, primarily proteins nucleic acids, into condensed, liquid-like droplets under specific conditions. LLPS drives formation biomolecular condensates, which are crucial for various functions. Increasing evidences link alterations to onset progression diseases, particularly review explores diverse roles cancer, highlighting its underlying mechanisms far-reaching implications. We examine how dysregulated contributes cancer development by influencing key processes such as genomic instability, metabolism, immune evasion. Furthermore, we discuss emerging therapeutic strategies aimed at modulating LLPS, underscoring their potential revolutionize treatment.

Language: Английский

Citations

0
Molecular mechanisms in regulation of autophagy and apoptosis in view of epigenetic regulation of genes and involvement of liquid-liquid phase separation DOI

Subhajit Chakraborty,

Piyasa Nandi,

Jagdish Mishra

et al.

Cancer Letters, Journal Year: 2024, Volume and Issue: 587, P. 216779 - 216779

Published: March 6, 2024

Language: Английский

Citations

3
Liquid–Liquid Phase Separation Sheds New Light upon Cardiovascular Diseases DOI Open Access

Ziyang Cai,

Shuai Mei,

Zhou Li

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(20), P. 15418 - 15418

Published: Oct. 21, 2023

Liquid-liquid phase separation (LLPS) is a biophysical process that mediates the precise and complex spatiotemporal coordination of cellular processes. Proteins nucleic acids are compartmentalized into micron-scale membrane-less droplets via LLPS. These droplets, termed biomolecular condensates, highly dynamic, have concentrated components, perform specific functions. Biomolecular condensates been observed to organize diverse key biological processes, including gene transcription, signal transduction, DNA damage repair, chromatin organization, autophagy. The dysregulation these activities owing aberrant LLPS important in cardiovascular diseases. This review provides detailed overview regulation functions comprehensive depiction several common diseases, discusses revolutionary therapeutic perspective modulating diseases new treatment strategies relevant

Language: Английский

Citations

4
The liquid-liquid phase separation in programmed cell death DOI

Leijing Yin,

Ludong Yuan, Jing Li

et al.

Cellular Signalling, Journal Year: 2024, Volume and Issue: 120, P. 111215 - 111215

Published: May 11, 2024

Language: Английский

Citations

1
Compartmentalized Biomolecular Condensates via Controlled Nucleation DOI Creative Commons
Chong Wang, Linyi Zhang, Luoran Shang

et al.

Research, Journal Year: 2024, Volume and Issue: 7

Published: Jan. 1, 2024

This commentary underscores the importance and implications of study “Biomolecular condensates with complex architectures via controlled nucleation,” led by Jan C. M. van Hest Tuomas P. J. Knowles, published in Nature Chemical Engineering . The research team developed a novel system to investigate structure biological using quaternized amylose, carboxymethylated single-stranded DNA. They successfully created multiphase droplets distinct dense phases demonstrated that droplet architecture can be through temperature salt concentration adjustments. offers valuable insights into formation function membraneless organelles cells suggests promising applications for designing biomimetic materials therapeutic strategies.

Language: Английский

Citations

0
Screening of liquid–liquid phase separation conditions for proteins with a mixed solution kit of biomacromolecular crowding agents DOI
Wan‐Yi Ge,

Wen‐Pu Shi,

Xueting Wang

et al.

Journal of Molecular Liquids, Journal Year: 2024, Volume and Issue: 406, P. 125038 - 125038

Published: May 19, 2024

Language: Английский

Citations

0
Growing a single suspended perfect protein crystal in a fully noncontact manner DOI

Zi-Qing Wu,

Yongming Liu,

Qing-Di Cheng

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 282, P. 136637 - 136637

Published: Oct. 30, 2024

Language: Английский

Citations

0
AH-6809 mediated regulation of lung adenocarcinoma metastasis through NLRP7 and prognostic analysis of key metastasis-related genes DOI Creative Commons
Feng Xu, Wei Wu, Feifei Liu

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 4, 2024

Lung adenocarcinoma (LUAD) has become one of the leading causes cancer-related deaths globally, with metastasis representing most lethal stage disease. Despite significant advances in diagnostic and therapeutic strategies for LUAD, mechanisms enabling cancer cells to breach blood-brain barrier remain poorly understood. While genomic profiling shed light on nature primary tumors, genetic drivers clinical relevance LUAD are still largely unexplored.

Language: Английский

Citations

0
Paromomycin targets HDAC1-mediated SUMOylation and IGF1R translocation in glioblastoma DOI Creative Commons
Min Zhong,

Yuejie Guo,

Ning Luo

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 11, 2024

Objective This study investigates the effects of Paromomycin on SUMOylation-related pathways in glioblastoma (GBM), specifically targeting HDAC1 inhibition. Methods Using TCGA and GTEx datasets, we identified genes associated with GBM prognosis. Molecular docking analysis suggested as a potential inhibitor. In vitro assays U-251MG cells were performed to assess Paromomycin’s cell viability, SUMOylation gene expression, IGF1R translocation using CCK8 assays, qRT-PCR, immunofluorescence. Results treatment led dose-dependent reduction colony formation, migration. It modulated SUMO1 expression decreased nuclear translocation, an effect reversible by inhibitor Trochostatin A (TSA), suggesting involvement SUMO1-regulated pathways. Conclusion highlights therapeutic agent for HDAC1-mediated influencing warranting further investigation its clinical application.

Language: Английский

Citations

0