3D cell culture models in research: applications to lung cancer pharmacology DOI Creative Commons
Nathan Vella, Anthony G. Fenech,

Vanessa Petroni Magri

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 23, 2024

Lung cancer remains one of the leading causes cancer-related mortality worldwide, necessitating innovative research methodologies to improve treatment outcomes and develop novel strategies. The advent three-dimensional (3D) cell cultures has marked a significant advancement in lung research, offering more physiologically relevant model compared traditional two-dimensional (2D) cultures. This review elucidates various types 3D culture models currently used pharmacology, including spheroids, organoids engineered tissue models, having pivotal roles enhancing our understanding biology, facilitating drug development, advancing precision medicine. systems mimic complex spatial architecture microenvironment tumours, providing critical insights into cellular molecular mechanisms tumour progression, metastasis responses. Spheroids, derived from commercialized lines, effectively (TME), formation hypoxic nutrient gradients, crucial for evaluating penetration efficacy anti-cancer therapeutics. Organoids tumouroids, primary tissues, recapitulate heterogeneity cancers are instrumental personalized medicine approaches, supporting simulation vivo pharmacological responses patient-specific context. Moreover, these have been co-cultured with biomimicry extracellular matrix (ECM) components further heterotypic cell-cell cell-ECM interactions present within TME. significantly contributing identification therapeutic targets resistance against conventional therapies. Therefore, this summarizes latest findings involving together common laboratory-based assays study effects. Additionally, integration development workflows is discussed. accelerating translation laboratory clinical applications, thereby landscape treatment. By closely mirroring human not only enhance disease but also pave way effective

Language: Английский

A concise review of VEGF, PDGF, FGF, Notch, angiopoietin, and HGF signalling in tumor angiogenesis with a focus on alternative approaches and future directions DOI
Selvaraj Vimalraj

International Journal of Biological Macromolecules, Journal Year: 2022, Volume and Issue: 221, P. 1428 - 1438

Published: Sept. 17, 2022

Language: Английский

Citations

98
The role of tumor-associated macrophages in tumor immune evasion DOI Creative Commons

Ruizhe Huang,

Ting Kang, Siyu Chen

et al.

Journal of Cancer Research and Clinical Oncology, Journal Year: 2024, Volume and Issue: 150(5)

Published: May 7, 2024

Abstract Background Tumor growth is closely linked to the activities of various cells in tumor microenvironment (TME), particularly immune cells. During progression, circulating monocytes and macrophages are recruited, altering TME accelerating growth. These adjust their functions response signals from stromal Tumor-associated (TAMs), similar M2 macrophages, key regulators TME. Methods We review origins, characteristics, TAMs within This analysis includes mechanisms through which facilitate evasion promote metastasis. Additionally, we explore potential therapeutic strategies that target TAMs. Results instrumental mediating malignant behaviors. They release cytokines inhibit effector attract additional immunosuppressive primarily T cells, inducing exhaustion directly, influencing activity indirectly cellular interactions, or suppressing checkpoints. directly involved proliferation, angiogenesis, invasion, Summary Developing innovative tumor-targeted therapies immunotherapeutic currently a promising focus oncology. Given pivotal role evasion, several approaches have been devised them. include leveraging epigenetics, metabolic reprogramming, engineering repolarize TAMs, inhibiting recruitment activity, using as drug delivery vehicles. Although some these remain distant clinical application, believe future targeting will offer significant benefits cancer patients.

Language: Английский

Citations

44
Flavonoids regulate tumor-associated macrophages – From structure-activity relationship to clinical potential (Review) DOI
Qi Sun, Qi Liu,

Xintong Zhou

et al.

Pharmacological Research, Journal Year: 2022, Volume and Issue: 184, P. 106419 - 106419

Published: Aug. 27, 2022

Language: Английский

Citations

53
Combined targeting of senescent cells and senescent macrophages: A new idea for integrated treatment of lung cancer DOI
Ming Gu, Yang Liu, Wenhui Zheng

et al.

Seminars in Cancer Biology, Journal Year: 2024, Volume and Issue: 106-107, P. 43 - 57

Published: Aug. 29, 2024

Language: Английский

Citations

11
Toward innovative approaches for exploring the mechanically regulated tumor-immune microenvironment DOI Creative Commons
Maria Kalli, Triantafyllos Stylianopoulos

APL Bioengineering, Journal Year: 2024, Volume and Issue: 8(1)

Published: Feb. 21, 2024

Within the complex tumor microenvironment, cells experience mechanical cues-such as extracellular matrix stiffening and elevation of solid stress, interstitial fluid pressure, shear stress-that significantly impact cancer cell behavior immune responses. Recognizing significance these cues not only sheds light on progression but also holds promise for identifying potential biomarkers that would predict therapeutic outcomes. However, standardizing methods studying how affect is challenging. This challenge stems from limitations traditional

Language: Английский

Citations

9
Microenvironment-based immunotherapy in oral cancer: a comprehensive review DOI

Hassan Mivehchi,

Aisan Eskandari-Yaghbastlo,

Masumeh Ghazanfarpour

et al.

Medical Oncology, Journal Year: 2025, Volume and Issue: 42(5)

Published: March 28, 2025

Language: Английский

Citations

1
SHH/GLI2-TGF-β1 feedback loop between cancer cells and tumor-associated macrophages maintains epithelial-mesenchymal transition and endoplasmic reticulum homeostasis in cholangiocarcinoma DOI Creative Commons

Zuxiao Chen,

Haiyan Li, Zongyan Li

et al.

Pharmacological Research, Journal Year: 2022, Volume and Issue: 187, P. 106564 - 106564

Published: Nov. 21, 2022

Tumor-associated macrophages (TAMs) play a dual role in tumors. However, the factors which drive function of TAMs cholangiocarcinoma remain largely undefined.SHH signaling pathway and endoplasmic reticulum stress (ERS) indicators were detected clinical tissues cell lines. co-cultured with cells under conditions hypoxia/normoxia. Polarized counted by flow cytometry, TGF-β1 levels supernatants ELISA. The effects glioma-associated oncogene GLI2 on themselves examined conducting interference overexpression assays.The SHH ERS both activated tumor or lines hypoxia. In co-culture experiments, presence increased proportion M2-polarized secretion TAMs, while knockdown expression reversed those increases. Overexpression TAMS stimulation Hh-Ag1.5 their expression. Furthermore, conditions, reduced migration, invasion, ER homeostasis induced Hh-Ag1.5-pretreated TAMs. Under hypoxia, promoted Tams, turn, inhibited apoptosis migration invasion cells. vivo, an injection plus contributed to growth, EMT, tissue, had opposite effects.Cholangiocarcinoma regulated TAM polarization via paracrine pathway, TGF-β1.

Language: Английский

Citations

31
Macrophage-based cancer immunotherapy: Challenges and opportunities DOI
Haotian Bai, Li Feng,

F Schmid

et al.

Experimental Cell Research, Journal Year: 2024, Volume and Issue: 442(1), P. 114198 - 114198

Published: Aug. 3, 2024

Language: Английский

Citations

6
CAR-macrophage: Breaking new ground in cellular immunotherapy DOI Creative Commons

Ting Huang,

Chenqi Bei,

Zhenhua Hu

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: Oct. 3, 2024

Chimeric Antigen Receptor (CAR) technology has revolutionized cellular immunotherapy, particularly with the success of CAR-T cells in treating hematologic malignancies. However, have limited efficacy against solid tumors. To address these limitations, CAR-macrophages (CAR-Ms) leverage innate properties macrophages specificity and potency CAR technology, offering a novel promising approach to cancer immunotherapy. Preclinical studies shown that CAR-Ms can effectively target destroy tumor cells, even within challenging microenvironments, by exhibiting direct cytotoxicity enhancing recruitment activation other immune cells. Additionally, favorable safety profile their persistence tumors position as potentially safer more durable therapeutic options compared This review explores recent advancements including engineering strategies optimize anti-tumor preclinical evidence supporting use. We also discuss challenges future directions developing therapies, emphasizing potential revolutionize By harnessing unique macrophages, offer groundbreaking overcoming current limitations cell paving way for effective sustainable treatments.

Language: Английский

Citations

6
Exosomal ncRNAs facilitate interactive ‘dialogue’ between tumor cells and tumor-associated macrophages DOI

Xintong Zhou,

Qi Liu, Xiaomin Wang

et al.

Cancer Letters, Journal Year: 2022, Volume and Issue: 552, P. 215975 - 215975

Published: Oct. 25, 2022

Language: Английский

Citations

23