Preliminary study based on methylation and transcriptome gene sequencing of lncRNAs and immune infiltration in hypopharyngeal carcinoma DOI Creative Commons

Kainan Wu,

Fen Chang,

Wenming Li

et al.

Frontiers in Oncology, Journal Year: 2023, Volume and Issue: 13

Published: April 25, 2023

Background Hypopharyngeal squamous cell cancer (HSCC) is one of the most malignant tumors head and neck. It not easy to detect in early stage due its hidden location; thus, lymph node metastasis highly likely at diagnosis, leading a poor prognosis. believed that epigenetic modification related invasion metastasis. However, role m6A-related lncRNA tumor microenvironment (TME) HSCC remains unclear. Methods The whole transcriptome methylation sequencing 5 pairs tissues adjacent were performed identify profiles lncRNAs. biological significance lncRNAs differentially expressing m6A peak was analyzed by Gene Ontology Kyoto Encyclopedia Genes Genomes. By constructing an lncRNA-microRNA network, mechanism analyzed. relative expression levels selected examined quantitative polymerase chain reaction. CIBERSORT algorithm used evaluate proportion immune infiltration paracancerous tissues. Results Based on in-depth analysis results, 14413 expressed revealed, including 7329 up-regulated 7084 down-regulated Additionally, 4542 up-methylated 2253 down-methylated detected. We demonstrated patterns gene transcriptome. In intersection methylated lncRNAs, 51 with 40 screened, significantly differentiated further studied. analysis, B memory elevated tissue, while γδT amount decreased. Conclusion might be involved pathogenesis. Infiltration cells provide new direction for treatment. This study provides insights exploring possible pathogenesis searching potential therapeutic targets.

Language: Английский

New insights into the interaction between m6A modification and lncRNA in cancer drug resistance DOI Creative Commons

Yizhou Jin,

Zhipeng Fan

Cell Proliferation, Journal Year: 2023, Volume and Issue: 57(4)

Published: Nov. 14, 2023

Abstract Drug resistance is perhaps the greatest obstacle in improving outcomes for cancer patients, leading to recurrence, progression and metastasis of various cancers. Exploring underlying mechanism worth further study. N6‐methyladenosine (m6A) most common RNA modification found eukaryotes, playing a vital role translation, transportation, stability, degradation, splicing processing. Long noncoding (lncRNA) refers group transcripts that are longer than 200 nucleotides (nt) typically lack ability code proteins. LncRNA has been identified play significant regulating multiple aspects tumour development progression, including proliferation, metastasis, metabolism, treatment. In recent years, growing body evidence emerged, highlighting crucial interplay between m6A lncRNA determining sensitivity cells chemotherapeutic agents. this review, we focus on advancements interaction modulation drug resistance. Additionally, aim explore mechanisms involved process. The objective review provide valuable insights suggest potential future directions reversal chemoresistance cancer.

Language: Английский

Citations

18
XIST and MUC1-C form an auto-regulatory pathway in driving cancer progression DOI Creative Commons

Keyi Wang,

Atrayee Bhattacharya, Naoki Haratake

et al.

Cell Death and Disease, Journal Year: 2024, Volume and Issue: 15(5)

Published: May 13, 2024

Abstract The long non-coding RNA X-inactive specific transcript (lncRNA XIST) and MUC1 gene are dysregulated in chronic inflammation cancer; however, there is no known interaction of their functions. present studies demonstrate that MUC1-C regulates XIST lncRNA levels by suppressing the RBM15/B, WTAP METTL3/14 components m6A methylation complex associate with A repeats. also suppresses YTHDF2-CNOT1 deadenylase recognizes sites contributes to decay increases stability expression. In support an auto-regulatory pathway, we show expression promoting NF-κB-mediated activation gene. Of significance, regulate common genes associated stemness, including (i) miR-21 which upregulated across pan-cancers, (ii) TDP-43 associates E Our results further MUC1-C/XIST pathway regulated TDP-43, drives stemness-associated genes, (iii) necessary for self-renewal capacity. These findings indicate axis importance cancer progression.

Language: Английский

Citations

7
The roles and mechanisms of coding and noncoding RNA variations in cancer DOI Creative Commons

Sang Yean Kim,

Min Jeong Na,

Sungpil Yoon

et al.

Experimental & Molecular Medicine, Journal Year: 2024, Volume and Issue: 56(9), P. 1909 - 1920

Published: Sept. 2, 2024

Functional variations in coding and noncoding RNAs are crucial tumorigenesis, with cancer-specific alterations often resulting from chemical modifications posttranscriptional processes mediated by enzymes. These RNA have been linked to tumor cell proliferation, growth, metastasis, drug resistance valuable for identifying diagnostic or prognostic cancer biomarkers. The diversity of modifications, such as splicing, polyadenylation, methylation, editing, is particularly significant due their prevalence impact on progression. Additionally, other including acetylation, circularization, miRNA isomerization, pseudouridination, recognized key contributors development. Understanding the mechanisms underlying these can enhance our knowledge biology facilitate development innovative therapeutic strategies. Targeting regulatory enzymes may pave way novel RNA-based therapies, enabling tailored interventions specific subtypes. This review provides a comprehensive overview roles various progression highlights recent advancements applications.

Language: Английский

Citations

4
Recent Advances in the Mutual Regulation of m6A Modification and Non-Coding RNAs in Atherosclerosis DOI Creative Commons

Yanlu Wang,

Shun M. Ling,

Hao Feng

et al.

International Journal of General Medicine, Journal Year: 2025, Volume and Issue: Volume 18, P. 1047 - 1073

Published: Feb. 1, 2025

Atherosclerosis, a progressive inflammatory disease of the arteries, remains leading cause cardiovascular morbidity and mortality worldwide. Recent years have witnessed pivotal role N6-methyladenosine (m6A) RNA methylation in regulating various biological processes, including those implicated atherosclerosis. Current evidence suggested that m6A regulators (writers, erasers, readers) participated modification multiple non-coding RNAs (ncRNAs), microRNAs (miRNAs), long (lncRNAs), circular (circRNAs), thereby affecting their metabolism functions. Meanwhile, ncRNAs also emerged as key modulator expression turn. Therefore, understanding mutual regulation between modifications is great significance to identify novel therapeutic targets for atherosclerosis has clinical application prospects. This review aims summarize recent advances reciprocal provide insights into interaction context

Language: Английский

Citations

0
RNA Modification in Metabolism DOI Creative Commons
Yadi Liu,

Zhongyan Sun,

Dingkun Gui

et al.

MedComm, Journal Year: 2025, Volume and Issue: 6(3)

Published: March 1, 2025

ABSTRACT Epigenetic regulation in disease development has been witnessed within this decade. RNA methylation is the predominant form of epigenetic regulation, and most prevalent modification N6‐methyladenosine (m 6 A). Recently, emerged as a potential target for treatment. posttranscriptional gene expression that involved both physiological pathological processes. Evidence suggests m A significantly affects metabolism, its abnormal changes have observed variety diseases. Metabolic diseases are series caused by metabolic processes body, common include diabetes mellitus, obesity, nonalcoholic fatty liver disease, etc.; although pathogenesis these differs from each other to current understanding, recent studies suggested pivotal role modulating diseases, A‐based drug on agenda. This paper reviewed understanding hoping provide systematic information those area.

Language: Английский

Citations

0
LncRNA SLC7A11-AS1 promotes the progression of hepatocellular carcinoma by mediating KLF9 ubiquitination DOI Open Access

Fan‐Lin Zeng,

Jie Lin,

Xing Xie

et al.

Neoplasma, Journal Year: 2023, Volume and Issue: 70(03), P. 361 - 374

Published: Jan. 1, 2023

Hepatocellular carcinoma (HCC) is a malignant tumor, which seriously threatens the life of patients. LncRNA SLC7A11-AS1 was reported to be abnormally expressed in HCC. Here, functions and relative molecular regulatory mechanism HCC were investigated. Nude mice cells used as experimental subjects. Knockdown or overexpression exogenous genes conducted cells. RT-qPCR, IHC, western blot employed evaluate abundance proteins. The behaviors evaluated using CCK-8, clone formation, wound-healing, Transwell. locations KLF9 determined by FISH IF assays. total m6A level dot-blot assay. modification detected RNA MeRIP. interactions among molecules validated RIP, ChIP, dual luciferase reporter assay, co-IP. elevated apparently tissues from mice. silencing could suppress progression, vivo vitro experiments. Furthermore, METTL3 mediated elevate its expression. In addition, downregulated expression affecting STUB1-mediated ubiquitination degradation enhanced PHLPP2 inactivate AKT pathway. Eventually, rescue experiments revealed that knockdown abolished silencing-mediated suppression progression vitro. Our results unveiled m6A-modified promoted regulating STUB1/KLF9/PHLPP2/AKT axis, indicating targeting might alleviate progression.

Language: Английский

Citations

8
BMP9-ID1 Pathway Attenuates N6-Methyladenosine Levels of CyclinD1 to Promote Cell Proliferation in Hepatocellular Carcinoma DOI Open Access
Han Chen, Mingming Zhang, Jianhao Li

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(2), P. 981 - 981

Published: Jan. 12, 2024

Hepatocellular carcinoma (HCC) is a highly lethal malignant neoplasm, and the involvement of bone morphogenetic protein 9 (BMP9) has been implicated in pathogenesis liver diseases HCC. Our goal was to investigate role BMP9 signaling regulating N6-methyladenosine (m

Language: Английский

Citations

1
Revealing a Novel Methylated Integrin Alpha-8 Related to Extracellular Matrix and Anoikis Resistance Using Proteomic Analysis in the Immune Microenvironment of Lung Adenocarcinoma DOI
Tingting Liu, Wen Ji, Xue Cheng

et al.

Molecular Biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: March 21, 2024

Language: Английский

Citations

1
The regulatory process and practical significance of non-coding RNA in the dissemination of prostate cancer to the skeletal system DOI Creative Commons

Hui Sang,

Luxi Li, Qiang Zhao

et al.

Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14

Published: March 21, 2024

Prostate cancer is a major contributor to male cancer-related mortality globally. It has particular affinity for the skeletal system with metastasis bones seriously impacting prognosis. The identification of prostate biomarkers can significantly enhance diagnosis and patient monitoring. Research found that metastases exhibit abnormal expression numerous non-coding RNA. Some these RNA facilitate bone by activating downstream signaling pathways, while others inhibit this process. Elucidating functional processes in will likely lead innovative treatment strategies malignant condition. In review, mechanistic role various examined. Our goal provide new avenue approach cancer.

Language: Английский

Citations

1
Characterization of N6-methyladenosine long non-coding RNAs in sporadic congenital cataract and age-related cataract DOI
Hongfei Ye, Xiang Zhang, Zhennan Zhao

et al.

International Journal of Ophthalmology, Journal Year: 2024, Volume and Issue: 17(11), P. 1973 - 1986

Published: Oct. 23, 2024

AIM: To characterize the N6-methyladenosine (m6A) modification patterns in long non-coding RNAs (lncRNAs) sporadic congenital cataract (CC) and age-related (ARC). METHODS: Anterior capsule of lens were collected from patients with CC ARC. Methylated RNA immunoprecipitation next-generation sequencing performed to identify m6A-tagged lncRNAs expression. Kyoto Encyclopedia Genes Genomes pathway enrichment analyses Gene Ontology annotation used predict potential functions m6A-lncRNAs. RESULTS: Large amount m6A peaks within lncRNA identified for both ARC, while level was much higher ARC (49 870 peaks) than that (18 688 peaks), yet those difference between younger age group (ARC-1) elder (ARC-2) quite slight. A total 1305 hypermethylated 1178 hypomethylated lncRNAs, as well 182 differential expressed exhibited compared CC. On other hand, 5893 5213 155 significantly altered ARC-2 ARC-1. Altered mainly associated organization biogenesis intracellular organelles, nucleotide excision repair. CONCLUSION: Our results first time present an overview methylomes providing a solid basis uncovering new insight reveal pathogenic mechanism

Language: Английский

Citations

0