Frontiers in Oncology,
Journal Year:
2023,
Volume and Issue:
13
Published: April 25, 2023
Background
Hypopharyngeal
squamous
cell
cancer
(HSCC)
is
one
of
the
most
malignant
tumors
head
and
neck.
It
not
easy
to
detect
in
early
stage
due
its
hidden
location;
thus,
lymph
node
metastasis
highly
likely
at
diagnosis,
leading
a
poor
prognosis.
believed
that
epigenetic
modification
related
invasion
metastasis.
However,
role
m6A-related
lncRNA
tumor
microenvironment
(TME)
HSCC
remains
unclear.
Methods
The
whole
transcriptome
methylation
sequencing
5
pairs
tissues
adjacent
were
performed
identify
profiles
lncRNAs.
biological
significance
lncRNAs
differentially
expressing
m6A
peak
was
analyzed
by
Gene
Ontology
Kyoto
Encyclopedia
Genes
Genomes.
By
constructing
an
lncRNA-microRNA
network,
mechanism
analyzed.
relative
expression
levels
selected
examined
quantitative
polymerase
chain
reaction.
CIBERSORT
algorithm
used
evaluate
proportion
immune
infiltration
paracancerous
tissues.
Results
Based
on
in-depth
analysis
results,
14413
expressed
revealed,
including
7329
up-regulated
7084
down-regulated
Additionally,
4542
up-methylated
2253
down-methylated
detected.
We
demonstrated
patterns
gene
transcriptome.
In
intersection
methylated
lncRNAs,
51
with
40
screened,
significantly
differentiated
further
studied.
analysis,
B
memory
elevated
tissue,
while
γδT
amount
decreased.
Conclusion
might
be
involved
pathogenesis.
Infiltration
cells
provide
new
direction
for
treatment.
This
study
provides
insights
exploring
possible
pathogenesis
searching
potential
therapeutic
targets.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 22, 2024
ABSTRACT
BACKGROUND
Pulmonary
hypertension
(PH)
represents
a
significant
cardiovascular
disorder
marked
by
both
functional
and
structural
alterations
within
the
pulmonary
vasculature.
Long
non-coding
RNAs
(lncRNAs)
have
been
closely
associated
with
pathogenesis
progression
of
PH.
Nonetheless,
precise
mechanisms
which
lncRNAs
interact
its
downstream
target
molecules
to
modulate
disease
remain
inadequately
elucidated.
METHODS
The
expression
levels
LINC00599
were
quantified
in
lung
tissues
mice
arterial
smooth
muscle
cells
(PASMCs)
under
hypoxic
conditions.
involvement
PH
vascular
remodeling
was
evaluated
through
vivo
studies.
To
investigate
influences
proliferation
human
PASMCs,
small
interfering
RNA
overexpression
plasmids
employed.
RESUITS
is
upregulated
medial
layer
arteries
experimental
models
PASMCs.
Administration
single
dose
lentivirus-mediated
shRNA
targeting
effectively
reverses
murine
models.
plays
critical
role
PASMC
modulating
stress
granule
formation
N6-methyladenosine
(m6A)
modification
promoting
via
liquid-liquid
phase
separation
myosin
heavy
chain
9.
Furthermore,
regulated
super-enhancer,
mediated
transcription
factor
ZNF263.
CONCLUSIONS
These
findings
demonstrate
for
first
time
that
LINC00599,
involved
separation,
facilitates
enhancing
artery
cells.
GRAPHIC
What
Is
New?
This
study
identified
as
regulator
m6A
pivotal
Relevant?
LncRNAs
are
intricately
modulation
molecules.
However,
it
remains
unclear
whether
contribute
forming
specific
subcellular
structures
these
some
studies
suggested
increased
presence
granules
PASMCs
implicated
hypertension.
In
this
study,
we
provide
evidence
promotes
development
proteins.
Our
indicate
binding
modified
m6A,
G3BP1
MYH9
suggests
regulation
lncRNA
may
represent
pathological
mechanism
underlying
Are
Pathophysiological
Implications?
We
found
elevated
Knockout
prevented
PH,
indicating
potential
bomarker
therapeutic
Medicine,
Journal Year:
2022,
Volume and Issue:
101(51), P. e32318 - e32318
Published: Dec. 23, 2022
Bladder
cancer
(BC)
is
among
the
most
frequent
cancers
globally.
Although
substantial
efforts
have
been
put
to
understand
its
pathogenesis,
underlying
molecular
mechanisms
not
fully
elucidated.
Frontiers in Oncology,
Journal Year:
2023,
Volume and Issue:
13
Published: April 25, 2023
Background
Hypopharyngeal
squamous
cell
cancer
(HSCC)
is
one
of
the
most
malignant
tumors
head
and
neck.
It
not
easy
to
detect
in
early
stage
due
its
hidden
location;
thus,
lymph
node
metastasis
highly
likely
at
diagnosis,
leading
a
poor
prognosis.
believed
that
epigenetic
modification
related
invasion
metastasis.
However,
role
m6A-related
lncRNA
tumor
microenvironment
(TME)
HSCC
remains
unclear.
Methods
The
whole
transcriptome
methylation
sequencing
5
pairs
tissues
adjacent
were
performed
identify
profiles
lncRNAs.
biological
significance
lncRNAs
differentially
expressing
m6A
peak
was
analyzed
by
Gene
Ontology
Kyoto
Encyclopedia
Genes
Genomes.
By
constructing
an
lncRNA-microRNA
network,
mechanism
analyzed.
relative
expression
levels
selected
examined
quantitative
polymerase
chain
reaction.
CIBERSORT
algorithm
used
evaluate
proportion
immune
infiltration
paracancerous
tissues.
Results
Based
on
in-depth
analysis
results,
14413
expressed
revealed,
including
7329
up-regulated
7084
down-regulated
Additionally,
4542
up-methylated
2253
down-methylated
detected.
We
demonstrated
patterns
gene
transcriptome.
In
intersection
methylated
lncRNAs,
51
with
40
screened,
significantly
differentiated
further
studied.
analysis,
B
memory
elevated
tissue,
while
γδT
amount
decreased.
Conclusion
might
be
involved
pathogenesis.
Infiltration
cells
provide
new
direction
for
treatment.
This
study
provides
insights
exploring
possible
pathogenesis
searching
potential
therapeutic
targets.
