Molecular Chaperonin HSP60: Current Understanding and Future Prospects

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(10), P. 5483 - 5483

Published: May 17, 2024

Molecular chaperones are highly conserved across evolution and play a crucial role in preserving protein homeostasis. The 60 kDa heat shock (HSP60), also referred to as chaperonin (Cpn60), resides within mitochondria is involved maintaining the organelle’s proteome integrity HSP60 family, encompassing Cpn60, plays diverse roles cellular processes, including folding, cell signaling, managing high-temperature stress. In prokaryotes, well understood GroEL/GroES complex, which forms double-ring cavity aids folding. eukaryotes, implicated numerous biological functions, like facilitating folding of native proteins influencing disease development processes. Notably, research highlights its critical involvement sustaining oxidative stress mitochondrial integrity. perturbation results loss activates apoptosis. Currently, clinical investigations progress explore targeting both vivo vitro various models. These studies aim enhance our comprehension mechanisms potentially harness therapeutic target for conditions, cancer, inflammatory disorders, neurodegenerative diseases. This review delves into functions regulating proteo-homeostasis, stress, ROS, apoptosis, implications diseases cancer neurodegeneration.

Language: Английский

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Advances in the study of CCT3 in malignant tumors: A review

Medicine, Journal Year: 2025, Volume and Issue: 104(6), P. e41069 - e41069

Published: Feb. 7, 2025

Malignant tumors are among the leading causes of death worldwide, with their underlying mechanisms remaining largely unclear. Tumorigenesis is a complex process involving multiple factors, genes, and pathways. Tumor cells characterized by abnormal proliferation, infiltration, invasion, metastasis. Improving tumor diagnosis rates identifying novel molecular therapeutic targets great significance for advancement modern medicine. Chaperonin containing TCP-1 subunit 3 (CCT3) one subunits chaperonin complex, chaperone involved in protein folding remodeling. CCT3 plays crucial role maintaining homeostasis, key substrates including tubulin actin. In recent years, has been reported to be abnormally expressed various cancers, correlating prognosis outcomes. this review, we summarize basic structure function CCT3, discuss development. Additionally, explore its potential applications cancer treatment.

Language: Английский

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Ai-driven prediction of cardio-oncology biomarkers through protein corona analysis

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: 509, P. 161134 - 161134

Published: March 1, 2025

Language: Английский

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Pan-cancer analysis reveals immunological and prognostic significance of CCT5 in human tumors

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 24, 2025

Abstract The chaperonin containing TCP1 subunit 5 (CCT5) is believed to function as a tumor driver. However, systematic pan-cancer analysis of CCT5 still lacking. Therefore, this study aimed identify the potential role in different types tumors. This comprehensively investigated gene expression, proteomic immune infiltration, DNA methylation, genetic alterations, correlation with TMB and MSI, drug sensitivity, enrichment analysis, prognostic significance 33 tumors based on TIMER2.0, GEPIA2, UALCAN, SMART, cBioPortal, GSCA databases, TCGAplot R package. results revealed significant overexpression most was significantly associated poor OS DFS types. Reduced promoter N-shore methylation CCT5, indicating its oncogenic epigenetic roles. Amplification common type alterations. Immune infiltration strong between cells. exhibited MSI KIRC STAD. Furthermore, associations cell cycle pathway various cellular functions. These findings suggested that might serve biomarker target for immunotherapy cancers.

Language: Английский

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Regulation of Epithelial and Endothelial Barriers by Molecular Chaperones

Cells, Journal Year: 2024, Volume and Issue: 13(5), P. 370 - 370

Published: Feb. 21, 2024

The integrity and permeability of epithelial endothelial barriers depend on the formation tight junctions, adherens a junction-associated cytoskeleton. establishment this junction–cytoskeletal module relies correct folding oligomerization its protein components. Molecular chaperones are known regulators complex in different cellular compartments. Mammalian cells possess an elaborate chaperone network consisting several hundred co-chaperones. Only small part has been linked, however, to regulation intercellular adhesions, systematic analysis functions at is lacking. This review describes mechanisms chaperone-assisted junctions. major focus heat shock chaperones, their co-chaperones, chaperonins since these molecules majority articles published chaperone-mediated control tissue barriers. discusses roles steady-state vascular as well disruption by pathogenic factors extracellular stressors. Since cytoskeletal coupling essential for junctional remodeling, assembly actomyosin cytoskeleton also discussed.

Language: Английский

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Development and Validation of an Interpretable Machine Learning Model for Early Prognosis Prediction in ICU Patients with Malignant Tumors and Hyperkalemia

Medicine, Journal Year: 2024, Volume and Issue: 103(30), P. e38747 - e38747

Published: July 26, 2024

This study aims to develop and validate a machine learning (ML) predictive model for assessing mortality in patients with malignant tumors hyperkalemia (MTH). We extracted data on MTH from the Medical Information Mart Intensive Care-IV, version 2.2 (MIMIC-IV v2.2) database. The dataset was split into training set (75%) validation (25%). used Least Absolute Shrinkage Selection Operator (LASSO) regression identify potential predictors, which included clinical laboratory indicators vital signs. Pearson correlation analysis tested between predictors. In-hospital death prediction target. Area Under Curve (AUC) accuracy of sets 7 ML algorithms were compared, optimal 1 selected model. calibration curve evaluate further. SHapley Additive exPlanations (SHAP) Local Interpretable Model-agnostic Explanations (LIME) enhanced interpretability. 496 Care Unit (ICU) included. After screening, 17 features construction model, coefficient <0.8, indicating that small. eXtreme Gradient Boosting (XGBoost) outperformed other algorithms, achieving perfect scores (accuracy: 1.000, AUC: 1.000) high 0.734, 0.733). curves indicated good SHAP identified top 8 factors: urine output, mean heart rate, maximum urea nitrogen, minimum oxygen saturation, blood pressure, total bilirubin, respiratory pH. In addition, LIME performed in-depth individual case analyses. demonstrates effectiveness methods predicting risk ICU MTH. It highlights importance predictors like output rate. significantly model's

Language: Английский

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Chaperonin containing TCP1 subunit 6A may activate Notch and Wnt pathways to facilitate the malignant behaviors and cancer stemness in oral squamous cell carcinoma

Cancer Biology & Therapy, Journal Year: 2023, Volume and Issue: 25(1)

Published: Dec. 12, 2023

Chaperonin containing TCP1 subunit 6A (CCT6A) was recently discovered to be involved in cancer pathogenesis and stemness; however, its role oral squamous cell carcinoma (OSCC) has not been reported. The current study aimed investigate the impact of CCT6A on OSCC malignant behaviors stemness explore potentially interreacted pathways. SCC-15 HSC-3 cells were transfected with plasmid loading control overexpression, knockout, or knockout. Wnt4 overexpression Notch1 plasmids into CCT6A-knockout cells. Cell proliferation, apoptosis, invasion, stemness, Notch, Wnt pathways detected both lines, whereas RNA sequencing only performed upregulated five including SCC-15, HSC-3, SAT, SCC-9, KON, compared that line. In cells, increased sphere formation, CD133, Sox2 expression, but decreased apoptosis; contrary, knockout exhibited an opposite effect above indexes. RNA-sequencing data revealed Notch CCT6A’effect functions. positively regulates Importantly, it shown activation attenuated survival, stemness. may promote behavior by activating

Language: Английский

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Research status of abnormal lipid metabolism and tumorigenesis and development

Applied Mathematics and Nonlinear Sciences, Journal Year: 2024, Volume and Issue: 9(1)

Published: Jan. 1, 2024

Abstract There is a close relationship between abnormal lipid metabolism and tumor malignant behavior, plays crucial role in developing tumors. Based on BiLSTM model, combined with Transformer Encoder CRF decoder, the article successfully constructed joint extraction model of medical literature, help Neo4j technology, built knowledge graph abnormality occurrence development. The study used data sources from WOSCC, CNKI, Wanfang, VIP, other databases as sources. It bibliometric analysis tools like CiteSpace VOS viewer to visualize analyze related studies in-depth. results showed that Oral Oncology UK ranked first abnormalities number publications, total 301 papers an impact factor 5.118. oncology increased 11 2010 414 2023, growth more than 36.64 times. By comprehensively analyzing current situation tumorigenesis development, this paper provides new ideas perspectives for cancer prevention, diagnosis, treatment mechanism. helps promote in-depth research development fields.

Language: Английский

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PDCL3 is a Novel Oncogenic Indicator in Hepatocellular Carcinoma using Comprehensive Bioinformatics Analysis and in vitro Validation

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: April 30, 2024

Hepatocellular carcinoma (HCC) is the prevalent form of liver cancer and third major cause cancer-induced mortality globally. Phosducin-like protein 3 (PDCL3) linked to onset evolution malignancies, but its role mechanism in HCC progression are still unclear. Here, address this issue, we performed multiple bioinformatics mining analysis through databases such as GEO TCGA screen for HCC-specific targets. Differential survival prognostic analyses revealed that PDCL3 was upregulated tissues, accompanied by poorer outcomes patients. mRNA expression were significantly higher cell lines HepG2, Hep3B, Huh7 than normal human hepatocytes MIHA. The assays with siRNA interference showed exhibited proliferation, migration, invasion capacity these cells. high-expression group presented different immune infiltration mutation features, compared low-expression group. Furthermore, enrichment identified Hippo pathway YAP expression. Hence, emphasized promotive HCC, thus providing reliable evidence PDCL3-targeted diagnosis therapeutic strategies.

Language: Английский

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The role of CCT5 in human tumors: a pan-cancer analysis

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 13, 2024

CCT5, a molecular chaperone protein, was analyzed in 33 different tumor types by pan-cancer analysis. This study investigated various aspects such as gene expression, proteomic immune infiltration, DNA methylation, genetic alterations, survival, enrichment analysis, and prognostic significance. The results showed that CCT5 is highly expressed most tumors, its overexpression associated with poor overall disease-free well prognosis types. Immune infiltration analysis revealed correlation between cancer-associated fibroblasts (CAFs), CD8 + T cells, NK the of patients significantly correlated expression these three tumor-infiltrating cells. suggested regulates number thereby affecting tumors. Enrichment association cell cycle RNA-binding pathways. reduced promoter N-shore indicating potential oncogenic epigenetic roles. These findings suggest may serve biomarker target for immunotherapy cancer proliferation development.

Language: Английский

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