International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(23), P. 16940 - 16940
Published: Nov. 29, 2023
The
reprogramming
of
retinal
pigment
epithelium
(RPE)
cells
into
(transdifferentiation)
lies
in
the
bases
regeneration
several
Urodela.
identification
key
genes
involved
this
process
helps
with
looking
for
approaches
to
prevention
and
treatment
RPE-related
degenerative
diseases
human
retina.
purpose
our
study
was
examine
transcriptome
changes
at
initial
stages
RPE
cell
adult
newt
Pleurodeles
waltl.
isolated
from
eye
samples
day
0,
4,
7
after
experimental
surgical
detachment
neural
retina
used
a
de
novo
assembly
through
RNA-Seq
method.
A
total
1019
transcripts
corresponding
differently
expressed
have
been
revealed
silico:
83
increased
expression
an
early
stage,
168
late
stage
reprogramming.
We
identified
up-regulation
classical
response
genes,
chaperones
co-chaperones,
regulation
protein
biosynthesis,
suppressors
oncogenes,
EMT-related
genes.
growth
proportion
down-regulated
ribosomal
translation-associated
Our
findings
contribute
revealing
molecular
mechanism
Cellular Signalling,
Journal Year:
2024,
Volume and Issue:
122, P. 111304 - 111304
Published: July 20, 2024
Acute
myeloid
leukemia
(AML)
is
a
prevalent
hematologic
malignancy
characterized
by
steady
rise
in
morbidity
and
mortality
rates
over
time.
The
upregulation
of
methyltransferase-like
14
(METTL14)
expression
AML
has
been
identified;
however,
its
specific
contributions
to
progression
underlying
molecular
mechanisms
have
yet
be
elucidated.
METTL14-bound
mRNAs
were
predicted
using
bioinformatics
methods,
analyzed,
screened
identify
T-complex
protein
1
(TCP1).
regulatory
impact
METTL14
on
TCP1
was
observed.
clinical
samples
assessed
quantitative
real-time
PCR
western
blot
analysis.
involvement
malignant
through
vitro
vivo
functional
assays.
String
database
utilized
for
predicting
proteins
that
interact
with
TCP1,
while
assays
immunoprecipitation
employed
validate
the
associated
signaling
pathways.
overexpression
upregulates
cells.
patients
exhibit
high
levels
expression.
Elevated
HL60
U937
cells
lead
increased
proliferation,
migration,
invasion,
inhibition
apoptosis,
vivo,
it
accelerates
proliferation
tumorigenesis.
Mechanistically,
modulates
influencing
transcript
stability
via
m6A
methylation,
thereby
regulating
Additionally,
PPP2R2C
potentially
serves
as
crucial
target
implicated
AML.
Upregulation
METTL14-mediated
modification
disease.
Therefore,
targeting
could
potential
therapeutic
strategy
enhance
treatment
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(23), P. 16940 - 16940
Published: Nov. 29, 2023
The
reprogramming
of
retinal
pigment
epithelium
(RPE)
cells
into
(transdifferentiation)
lies
in
the
bases
regeneration
several
Urodela.
identification
key
genes
involved
this
process
helps
with
looking
for
approaches
to
prevention
and
treatment
RPE-related
degenerative
diseases
human
retina.
purpose
our
study
was
examine
transcriptome
changes
at
initial
stages
RPE
cell
adult
newt
Pleurodeles
waltl.
isolated
from
eye
samples
day
0,
4,
7
after
experimental
surgical
detachment
neural
retina
used
a
de
novo
assembly
through
RNA-Seq
method.
A
total
1019
transcripts
corresponding
differently
expressed
have
been
revealed
silico:
83
increased
expression
an
early
stage,
168
late
stage
reprogramming.
We
identified
up-regulation
classical
response
genes,
chaperones
co-chaperones,
regulation
protein
biosynthesis,
suppressors
oncogenes,
EMT-related
genes.
growth
proportion
down-regulated
ribosomal
translation-associated
Our
findings
contribute
revealing
molecular
mechanism
