Effects of Fisetin Treatment on Cellular Senescence of Various Tissues and Organs of Old Sheep

Antioxidants, Journal Year: 2023, Volume and Issue: 12(8), P. 1646 - 1646

Published: Aug. 21, 2023

Fisetin has been shown to be beneficial for brain injury and age-related disease via different mechanisms. The purpose of this study was determine the presence senescent cells effects fisetin on cellular senescence in other vital organs old sheep, a more translational model. Female sheep 6–7 years (N = 6) were treated with 100 mg/kg or vehicle alone two consecutive days week 8 weeks. All harvested at time sacrifice. Histology, immunofluorescence staining, RT-Q-PCR performed regions tissues organs. Our results indicated that treatment current regimen did not affect general morphology brain. both cerebral cortex cerebellum non-Cornu Ammonis (CA) area hippocampus detected by senescent-associated β-galactosidase (SA-β-Gal) staining GL13 (lipofuscin) staining. mainly neurons gray white matter either cortex, cerebellum, non-CA hippocampus. Very few CA1-4 hippocampus, as revealed GLB1 colocalization NEUN. significantly decreased number SA-β-Gal+ GL13+ showed decreasing trend cerebellum. Furthermore, P16+ GLB1+ neuronal nuclear protein (NEUN)+ neurons, glial fibrillary acidic (GFAP)+ astrocytes, ionized calcium binding adaptor molecule 1 (IBA1)+ microglia cortex. cells, NEUN+ plasma S100B. At mRNA level, downregulated liver, lung, heart, spleen tissues, P21 expression liver lung. TREM2 lung downregulation spleen, heart. A significant decrease NRLP3 observed after treatment. Finally, SOD1 while upregulating CAT spleen. In conclusion, we found widely present sheep. addition, gene expressions inflammasomes organs, such liver. represents promising therapeutic strategy diseases.

Language: Английский

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Fisetin as a senotherapeutic agent: Evidence and perspectives for age-related diseases

Mechanisms of Ageing and Development, Journal Year: 2024, Volume and Issue: 222, P. 111995 - 111995

Published: Oct. 9, 2024

Fisetin, a flavonoid naturally occurring in plants, fruits, and vegetables, has recently gained attention for its potential role as senotherapeutic agent the treatment of age-related chronic diseases. Senotherapeutics target senescent cells, which accumulate with age disease, both circulating immune cell populations solid organs tissues. Senescent cells contribute to development many diseases, primarily by eliciting systemic inflammation through their senescence-associated secretory phenotype. Here, we explore whether fisetin can eliminate thereby alleviate examining current evidence from vitro studies animal models that investigate fisetin's impact on well phase I/II trials various patient populations. We discuss application humans, including challenges future directions. Our review available data suggests targeting offers promising strategy managing multiple potentially transforming healthcare older multimorbid patients. However, further are needed establish safety, pharmacokinetics, efficacy senotherapeutic, identify relevant reliable outcome measures human trials, optimize dosing, better understand possible limitations agent.

Language: Английский

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The protective effects of fisetin in metabolic disorders: a focus on oxidative stress and associated events

Journal of Diabetes & Metabolic Disorders, Journal Year: 2024, Volume and Issue: 23(2), P. 1753 - 1771

Published: Oct. 21, 2024

Language: Английский

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Effects of Fisetin Treatment on Cellular Senescence of the Brain and Other Multiple Organs of Old Sheep

Published: June 27, 2023

Fisetin has been shown to be beneficial for brain injury and age-related disease via different mechanisms. The purpose of this study was determine the presence senescent cells effects fisetin on cellular senescence in other organs old sheep, a more translational model. Approximately 6-7 years female sheep (N=6) were treated with 100mg/kg or vehicle two consecutive days week 8 weeks. All harvested at time sacrifice. Histology, immunofluorescent staining, as well Q-PCR performed regions tissues organs. Our results indicated that treatment current regimen did not affect general morphology brain. both cerebral cortex cerebellum detected by SA-β-Gal staining. More observed gray matter when compared white cortex. These are mainly neurons either cerebellum. showed trend decrease significantly decreased matter. Furthermore, P16+ NEUN+ neurons, GFAP+ astrocytes, IBA+ microglia also astrocytes non- (Cornu Ammonis) CA area hippocampus. However, change CA1-4 At mRNA level, GLB1 heart ventricle muscle tissue spleen but tested. antioxidant gene SOD1 increased CAT bone marrow. variable SASP inflammasome genes In conclusion, we found widely present sheep. addition, non-CA represents promising therapeutic strategy disease.

Language: Английский

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Effects and Mechanisms of Fisetin against Ischemia-reperfusion Injuries: A Systematic Review

Current Pharmaceutical Biotechnology, Journal Year: 2024, Volume and Issue: 25(16), P. 2138 - 2153

Published: Feb. 4, 2024

Ischemia-reperfusion injury (IRI) is a well-known ailment that can disturb organ function.

Language: Английский

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An Insight into Fisetin, The Miraculous Multifaceted Flavonol: Paving the Road for Enhanced Delivery Through Promising Pharmaceutical Nano-Formulations.

Journal of Drug Delivery Science and Technology, Journal Year: 2024, Volume and Issue: unknown, P. 106292 - 106292

Published: Oct. 1, 2024

Language: Английский

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Natural Products Act as Game-Changer Potentially in Treatment and Management of Sepsis-Mediated Inflammation: A Clinical Perspective

Phytomedicine, Journal Year: 2024, Volume and Issue: 130, P. 155710 - 155710

Published: May 3, 2024

Language: Английский

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