The role of Exosomal miRNAs in cancer DOI Creative Commons
Chuanyun Li, Tong Zhou, Jing Chen

et al.

Journal of Translational Medicine, Journal Year: 2022, Volume and Issue: 20(1)

Published: Jan. 3, 2022

Exosomal miRNAs have attracted much attention due to their critical role in regulating genes and the altered expression of virtually all cancers affecting humans (Sun et al. Mol Cancer 17(1):14, 2018). modulate processes that interfere with cancer immunity microenvironment, are significantly involved tumor growth, invasion, metastasis, angiogenesis drug resistance. Fully investigating detailed mechanism occurrence development various could help not only treatment but also prevention malignant diseases. The current review highlighted recently published advances regarding cancer-derived exosomes, e.g., sorting delivery mechanisms for RNAs. cell-to-cell communication, impacting angiogenesis, metastasis multiple biological features, were discussed. Finally, potential exosomal as diagnostic prognostic molecular markers was summarized, well usefulness detecting resistance therapeutic agents.

Language: Английский

Overview of MicroRNA Biogenesis, Mechanisms of Actions, and Circulation DOI Creative Commons
Jacob A. O’Brien, Heyam Hayder,

Yara Zayed

et al.

Frontiers in Endocrinology, Journal Year: 2018, Volume and Issue: 9

Published: Aug. 3, 2018

MicroRNAs (miRNAs) are a class of non-coding RNAs that play important roles in regulating gene expression. The majority miRNAs transcribed from genes into primary and processed precursor finally mature miRNAs. In most cases, interact with the 3' UTR target mRNAs to induce mRNA degradation translational repression. However, interaction other regions, including 5' UTR, coding sequence, promoters, have also been reported. Under certain conditions, can activate translation or regulate transcription. their is dynamic dependent on many factors, such as subcellular location miRNAs, abundancy mRNAs, affinity miRNA-mRNA interactions. be secreted extracellular fluids transported cells via vesicles, exosomes, by binding proteins, Argonautes. Extracellular function chemical messengers mediate cell-cell communication. this review, we provide an update canonical non-canonical miRNA biogenesis pathways various mechanisms underlying miRNA-mediated regulations. We summarize current knowledge dynamics action secretion, transfer, uptake

Language: Английский

Citations

4215
Using exosomes, naturally-equipped nanocarriers, for drug delivery DOI
Elena V. Batrakova, Myoung Soo Kim

Journal of Controlled Release, Journal Year: 2015, Volume and Issue: 219, P. 396 - 405

Published: Aug. 1, 2015

Language: Английский

Citations

941
Effect of exosomal miRNA on cancer biology and clinical applications DOI Creative Commons
Zhenqiang Sun, Ke Shi,

Shuaixi Yang

et al.

Molecular Cancer, Journal Year: 2018, Volume and Issue: 17(1)

Published: Oct. 11, 2018

Exosomes, extracellular vesicles with diameters ranging from 30 to 150 nm, are widely present in various body fluids. Recently, microRNAs (miRNAs) have been identified exosomes, the biogenesis, release, and uptake of which may involve endosomal sorting complex required for transport (ESCRT complex) relevant proteins. After exosomes taken up by neighboring or distant cells, miRNAs contained within modulate such processes as interfering tumor immunity microenvironment, possibly facilitating growth, invasion, metastasis, angiogenesis drug resistance. Therefore, exosomal a significant function regulating cancer progression. Here, we briefly review recent findings regarding tumor-derived including RNA delivering mechanism. We then describe intercommunication occurring between different cells via microenvironmnt, impacts on proliferation, vascularization, metastasis other biological characteristics. Finally, highlight potential role these molecules biomarkers diagnosis prognosis resistance therapeutics.

Language: Английский

Citations

691
Exosome-delivered microRNAs modulate the inflammatory response to endotoxin DOI Creative Commons
Margaret Alexander,

Ruozhen Hu,

Marah C. Runtsch

et al.

Nature Communications, Journal Year: 2015, Volume and Issue: 6(1)

Published: June 18, 2015

Abstract MicroRNAs regulate gene expression posttranscriptionally and function within the cells in which they are transcribed. However, recent evidence suggests that microRNAs can be transferred between mediate target repression. We find endogenous miR-155 miR-146a, two critical inflammation, released from dendritic exosomes subsequently taken up by recipient cells. Following uptake, exogenous repression reprogramme cellular response to endotoxin, where exosome-delivered enhances while miR-146a reduces inflammatory expression. also present pass immune vivo , as well demonstrate exosomal inhibits promotes endotoxin-induced inflammation mice. Together, our findings provide strong undergo a functional transfer constitute mechanism of regulating response.

Language: Английский

Citations

663
MicroRNAome genome: A treasure for cancer diagnosis and therapy DOI
Ioana Berindan‐Neagoe,

Paloma del C. Monroig,

Barbara Pasculli

et al.

CA A Cancer Journal for Clinicians, Journal Year: 2014, Volume and Issue: 64(5), P. 311 - 336

Published: Aug. 7, 2014

Abstract The interplay between abnormalities in genes coding for proteins and noncoding microRNAs (miRNAs) has been among the most exciting yet unexpected discoveries oncology over last decade. complexity of this network redefined cancer research as miRNAs, produced from what was once considered “genomic trash,” have shown to be crucial initiation, progression, dissemination. Naturally occurring miRNAs are very short transcripts that never produce a protein or amino acid chain, but act by regulating expression during cellular processes such growth, development, differentiation at transcriptional, posttranscriptional, and/or translational level. In review article, presented ubiquitous players involved all hallmarks. authors also describe used methods detect their expression, which revealed identity hundreds dysregulated cells tumor microenvironment cells. Furthermore, role hormones reliable biomarkers predictors treatment response is discussed. Along with this, explore current strategies designing miRNA‐targeting therapeutics, well associated challenges envisions overcome. Finally, new wave molecular introduced: study long RNAs. CA Cancer J Clin 2014;64:311–336. © 2014 American Society.

Language: Английский

Citations

489
Circulating MicroRNAs in Cancer: Potential and Challenge DOI Creative Commons
Mengying Cui, Hongdan Wang,

Xiaoxiao Yao

et al.

Frontiers in Genetics, Journal Year: 2019, Volume and Issue: 10

Published: July 18, 2019

MicroRNAs (miRNAs) are endogenous non-coding small RNA molecules that can be secreted into the circulation and exist in remarkably stable forms. Like intercellular miRNAs, circulating miRNAs participate numerous regulations of biological process expressed aberrantly under abnormal or pathological status. The quality quantity changes associated with initiation progression cancer easily detected by basic molecular biology techniques. Consequently, considerable effort has been devoted to identify suitable extracellular for noninvasive biomarkers cancer. However, several challenges need overcome before practical application. In this review, we discuss issues miRNAs: function transport carriers; cell communication process; roles as reliable usage targeted therapy; clinical

Language: Английский

Citations

386
The potential of endurance exercise-derived exosomes to treat metabolic diseases DOI
Adeel Safdar, Ayesha Saleem, Mark A. Tarnopolsky

et al.

Nature Reviews Endocrinology, Journal Year: 2016, Volume and Issue: 12(9), P. 504 - 517

Published: May 27, 2016

Language: Английский

Citations

368
Gap junctions and cancer: communicating for 50 years DOI
Trond Aasen, Marc Mesnil, Christian C. Naus

et al.

Nature reviews. Cancer, Journal Year: 2016, Volume and Issue: 16(12), P. 775 - 788

Published: Oct. 21, 2016

Language: Английский

Citations

326
Macrophages derived exosomes deliver miR-223 to epithelial ovarian cancer cells to elicit a chemoresistant phenotype DOI Creative Commons
Xiaolan Zhu, Huiling Shen, Xinming Yin

et al.

Journal of Experimental & Clinical Cancer Research, Journal Year: 2019, Volume and Issue: 38(1)

Published: Feb. 15, 2019

How exosomal microRNAs (miRNAs) derived from macrophages contribute to the development of drug resistance in context hypoxic tumor microenvironment epithelial ovarian cancer (EOC) remains poorly understood.The miRNA levels were detected by qRT-PCR. Protein HIF-1α, CD163 and PTEN-PI3K/AKT pathway assessed Western blot (WB) Immunohistochemistry (IHC). Exosomes isolated, then confirmed Transmission electron microscopy (TEM), Nanoparticle Tracking Analysis (NTA) WB. Internalization macrophages-secreted exosomes EOC cells was Confocal microscope. Subsequently, Dual-luciferase reporter assay verified PTEN target miR-223. Gain- loss-of-function experiments, rescue SKOV3 xenograft models performed uncover underlying mechanisms miR-223 pathway, as well inducing multidrug vitro vivo.Here, we showed triggered recruitment induced into a tumor-associated macrophage (TAM)-like phenotype; enhanced malignant phenotype cells, enriched released under hypoxia, which could be transferred co-cultivated accompanied resistant cells. Besides, results functional revealed that promoted via both vivo vitro. Furthermore, patients with high HIF-1a expression had statistically higher CD163+ cell infiltration intertumoral Finally, circulating closely related recurrence EOC.These data indicate unique role cross-talk between chemotherapy resistance, through novel miR-223/PTEN-PI3K/AKT signaling pathway.

Language: Английский

Citations

323
Circulating miRNAs: Roles in cancer diagnosis, prognosis and therapy DOI

Guofeng Cheng

Advanced Drug Delivery Reviews, Journal Year: 2014, Volume and Issue: 81, P. 75 - 93

Published: Sept. 16, 2014

Language: Английский

Citations

317