Communication by Extracellular Vesicles: Where We Are and Where We Need to Go

Cell, Journal Year: 2016, Volume and Issue: 164(6), P. 1226 - 1232

Published: March 1, 2016

Language: Английский

---

RNA delivery by extracellular vesicles in mammalian cells and its applications

Nature Reviews Molecular Cell Biology, Journal Year: 2020, Volume and Issue: 21(10), P. 585 - 606

Published: May 26, 2020

Language: Английский

---

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Journal of Extracellular Vesicles, Journal Year: 2024, Volume and Issue: 13(2)

Published: Feb. 1, 2024

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of cell origin and change functions phenotypes other cells. These features indicate strong biomarker therapeutic potential have generated broad interest, as evidenced by steady year-on-year increase in numbers scientific publications about EVs. Important advances been made EV metrology understanding applying biology. However, hurdles remain to realising EVs domains ranging from basic biology clinical applications due challenges nomenclature, separation non-vesicular extracellular particles, characterisation functional studies. To address opportunities this rapidly evolving field, International Society for Vesicles (ISEV) updates its 'Minimal Information Studies Vesicles', which was first published 2014 then 2018 MISEV2014 MISEV2018, respectively. The goal current document, MISEV2023, is provide researchers with an updated snapshot available approaches advantages limitations production, multiple sources, including culture, body fluids solid tissues. In addition presenting latest art principles research, document also covers advanced techniques that are currently expanding boundaries field. MISEV2023 includes new sections on release uptake a brief discussion vivo study Compiling feedback ISEV expert task forces more than 1000 researchers, conveys research facilitate robust discoveries move field forward even rapidly.

Language: Английский

---

Exosomes: Therapy delivery tools and biomarkers of diseases

Pharmacology & Therapeutics, Journal Year: 2017, Volume and Issue: 174, P. 63 - 78

Published: Feb. 13, 2017

Language: Английский

---

Cells release subpopulations of exosomes with distinct molecular and biological properties

Scientific Reports, Journal Year: 2016, Volume and Issue: 6(1)

Published: March 2, 2016

Abstract Cells release nano-sized membrane vesicles that are involved in intercellular communication by transferring biological information between cells. It is generally accepted cells at least three types of extracellular (EVs): apoptotic bodies, microvesicles and exosomes. While a wide range putative functions have been attributed to exosomes, they assumed represent homogenous population EVs. We hypothesized the existence subpopulations exosomes with defined molecular compositions properties. Density gradient centrifugation isolated revealed presence two distinct subpopulations, differing biophysical properties their proteomic RNA repertoires. Interestingly, mediated differential effects on gene expression programmes recipient In conclusion, we demonstrate exosome unique elicit Further dissection heterogeneity will advance our understanding exosomal biology health disease accelerate development exosome-based diagnostics therapeutics.

Language: Английский

---

Extracellular Vesicle Heterogeneity: Subpopulations, Isolation Techniques, and Diverse Functions in Cancer Progression

Frontiers in Immunology, Journal Year: 2018, Volume and Issue: 9

Published: April 30, 2018

Cells release membrane enclosed nano-sized vesicles termed extracellular (EVs) that function as mediators of intercellular communication by transferring biological information between cells. Tumor-derived EVs have emerged important in cancer development and progression, mainly through transfer their bioactive content which can include oncoproteins, oncogenes, chemokine receptors, well soluble factors, transcripts proteins miRNAs involved angiogenesis or inflammation. This has been shown to influence the metastatic behavior primary tumors. Moreover, tumor-derived distant cellular niches, establishing favorable microenvironments support growth disseminated cells upon arrival at these pre-metastatic niches. It is generally accepted a number major EV populations with distinct biophysical properties functions. Exosomes, microvesicles, apoptotic bodies are most widely studied characterized. They discriminated based primarily on intracellular origin. However, increasing evidence suggests even within various subpopulations may exist. heterogeneity introduces an extra level complexity study biology function. For example, could unique roles intricate processes underlying biology. Here, we discuss current knowledge regarding role progression highlight relevance heterogeneity. The position tetraspanins integrins therein will be highlighted. Since addressing become essential for field, novel techniques isolating also discussed. Further dissection advance our understanding critical health disease.

Language: Английский

---

Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper

Journal of Extracellular Vesicles, Journal Year: 2017, Volume and Issue: 6(1)

Published: March 7, 2017

The release of RNA-containing extracellular vesicles (EV) into the milieu has been demonstrated in a multitude different vitro cell systems and variety body fluids. EV are limelight for their capacity to communicate genetically encoded messages other cells, suitability as candidate biomarkers diseases, use therapeutic agents. Although EV-RNA attracted enormous interest from basic researchers, clinicians, industry, we currently have limited knowledge on which mechanisms drive regulate RNA incorporation how RNA-encoded affect signalling processes EV-targeted cells. Moreover, research faces various technical challenges, such standardisation isolation methods, optimisation methodologies isolate characterise minute quantities found EV, development approaches demonstrate functional transfer vivo. These topics were discussed at 2015 workshop International Society Extracellular Vesicles. This position paper was written by participants not only give an overview current state field, but also clarify that our incomplete - nature EV(-RNA)s effectively reliably study them prohibits implementation gold standards research. In addition, this creates awareness possibilities limitations used strategies investigate calls caution interpretation obtained data.

Language: Английский

---

Re-Engineering Extracellular Vesicles as Smart Nanoscale Therapeutics

ACS Nano, Journal Year: 2017, Volume and Issue: 11(1), P. 69 - 83

Published: Jan. 9, 2017

In the past decade, extracellular vesicles (EVs) have emerged as a key cell-free strategy for treatment of range pathologies, including cancer, myocardial infarction, and inflammatory diseases. Indeed, field is rapidly transitioning from promising in vitro reports toward vivo animal models early clinical studies. These investigations exploit high physicochemical stability biocompatibility EVs well their innate capacity to communicate with cells via signal transduction membrane fusion. This review focuses on methods which can be chemically or biologically modified broaden, alter, enhance therapeutic capability. We examine two broad strategies, been used introduce wide nanoparticles, reporter systems, targeting peptides, pharmaceutics, functional RNA molecules. First, we explore how by manipulating parent cells, either through genetic metabolic engineering introducing exogenous material that subsequently incorporated into secreted EVs. Second, consider directly functionalized using strategies such hydrophobic insertion, covalent surface chemistry, permeabilization. discuss historical context each specific technology, present prominent examples, evaluate complexities, potential pitfalls, opportunities presented different re-engineering strategies.

Language: Английский

---

Extracellular Vesicles Provide a Means for Tissue Crosstalk during Exercise

Cell Metabolism, Journal Year: 2018, Volume and Issue: 27(1), P. 237 - 251.e4

Published: Jan. 1, 2018

Language: Английский

---

Microfluidics for exosome isolation and analysis: enabling liquid biopsy for personalized medicine

Lab on a Chip, Journal Year: 2017, Volume and Issue: 17(21), P. 3558 - 3577

Published: Jan. 1, 2017

Breakthroughs in adapting microfluidic systems for exosome isolation, detection, and analysis are providing new tools to revolutionize personalized medicine.

Language: Английский

---