Veterinary Sciences,
Journal Year:
2024,
Volume and Issue:
11(7), P. 326 - 326
Published: July 18, 2024
The
aims
of
this
study
were
to
characterize
the
semen
as
well
influence
breed,
season,
and
processing
on
spermatozoa
(SPZ)
traits
four
native
Portuguese
goat
breeds
used
for
bank
animal
germplasm
(BPAG).
A
total
1017
ejaculates
from
Serrana
(n
=
30),
Bravia
15),
Charnequeira
11),
Preta
de
Montezinho
3)
bucks
collected
between
2004
2020
at
(EZN-INIAV;
39°
N)
during
whole
year
under
natural
conditions.
All
fresh
cryopreserved
(−196
°C)
was
evaluated
stored
in
BPAG.
(the
smallest
breed)
produced
less
(p
<
0.05)
volume
ejaculate
than
all
other
breeds,
which
higher
full
breeding
season
(September–January;
p
0.05),
regarding
breeds.
Contrarily,
general,
SPZ
concentration
lower
September–January,
but
per
remained
similar
>
May–August
September–January
bucks.
viability
midpiece
defects
slightly
influenced
by
breed
head
(lowest
%
February–April;
0.05).
On
contrary,
freezing–thawing
cycle
strongly
0.01)
traits.
correlation
coefficients
these
thawed
low
(up
0.33;
0.01),
highlighting
importance
cryopreservation.
We
conclude
that
had
a
relevant
effect
traits,
it
much
evident
studied
These
goats
can
serve
donors
throughout
year,
Journal of Animal and Feed Sciences,
Journal Year:
2020,
Volume and Issue:
29(3), P. 189 - 198
Published: Sept. 21, 2020
1.
Acosta
A.A.,
Kruger
T.F.
(Editors),
1996.
Human
Spermatozoa
in
Assisted
Reproduction.
Parthenon
Publishing
Group.
New
York,
NY
(USA).
Google
Scholar
Biology,
Journal Year:
2021,
Volume and Issue:
10(7), P. 595 - 595
Published: June 28, 2021
The
aim
was
to
determine
the
relationship
between
kinematic
parameters
of
boar
spermatozoa
and
fertility
rates
sow,
as
well
assess
effect
sperm
clusters
on
capacity
ejaculate.
Semen
samples
were
collected
from
11
sexually
mature
boars.
Samples
analyzed
by
an
ISAS®v1
CASA-Mot
system
for
eight
parameters.
Ejaculate
characterized
using
multivariate
procedures,
such
principal
factors
(PFs)
analysis
clustering
methods
(the
k-means
model).
Four
different
ejaculate
identified
two
PFs
which
involved
linear
trajectory
velocity.
There
differences
(p
<
0.05)
variables
sire
line.
no
statistical
difference
>
dam
lines
in
variables.
discriminant
ability
kinematics
predict
litter
size
receiver
operating
characteristics
(ROC)
curve
analysis.
Curvilinear
velocity
(VCL),
average
path
(VAP),
amplitude
lateral
head
displacement
(ALH),
beat-cross
frequency
(BCF)
showed
significant,
albeit
limited,
predictive
(range:
0.55–0.58
area
under
curve,
AUC).
ejaculates
did
not
have
a
Revista de Biología Tropical,
Journal Year:
2019,
Volume and Issue:
67(6)
Published: Nov. 6, 2019
Sperm
kinematics
and
morphometric
subpopulations
analysis
with
CASA
systems:
a
review.
The
subjective
evaluation
of
seminal
quality
has
given
way
to
the
use
objective
assessment
techniques
by
technology
(computer-assisted
semen
analysis).
application
principal
components
(PC)
clustering
methods
reveal
spermatozoa
is
powerful
tool
evaluate
raw
processed
cell
suspensions,
but
not
many
researchers
are
aware
technique.
PC
multivariate
statistical
method
that
reduces
number
variables
used
in
subsequent
calculations
describe
data.
By
integrating
original
according
their
coherence
database
into
new
complex
mathematical
variable,
clearly
defined
homogenous
can
be
defined.
Kinematic,
morphometric,
morphological
or
DNA
integrity
tests
may
apply
characterize
it
understand
reproductive
biology
spermatozoon.
In
recent
years
there
been
substantive
change
conceptual
paradigm
regarding
what
an
ejaculate
is.
It
should
said
until
recently
was
considered
representative
population
(billions)
sperm
made
up
"equivalent"
cells
common
goal:
one
could
finally
fertilize
oocyte.
verification
set
grouped
distinct
subpopulations,
kinematic
characteristics,
opens
towards
more
cooperative
vision.
Besides,
we
have
seen
subpopulation
distribution
different
among
males,
which
seems
indicate
strategies
understood
within
another
paradigm,
competition
between
ejaculates.
existence
heterogeneous
show
patterns
widely
known
biological
meaning
these
still
clear.
Although
role
remains
unknown,
work
continue
direction.
PLoS ONE,
Journal Year:
2021,
Volume and Issue:
16(9), P. e0256701 - e0256701
Published: Sept. 2, 2021
The
developmental
competence
of
male
and
female
gametes
is
frequently
reduced
under
in
vitro
conditions,
mainly
due
to
oxidative
stress
during
handling.
amino-acid
derived
hormone
melatonin
has
emerged
as
a
potent
non-enzymatic
antioxidant
many
biological
systems.
goal
the
present
study
was
evaluate
effects
on
post-thaw
sperm
quality,
fertilizing
ability,
embryo
development
after
fertilization.
Frozen-thawed
bovine
spermatozoa
were
incubated
either
presence
10
−11
M
(MT),
or
its
solvent
(ethanol;
Sham-Control),
plain
Tyrode’s
Albumin
Lactate
Pyruvate
medium
(TALP,
Control).
Computer-Assisted
Sperm
Analysis
(CASA)
flow
cytometry
data
30
min,
120
180
min
incubation
did
not
reveal
any
significant
average
motility
parameters,
subpopulation
structure
determined
by
hierarchical
cluster,
percentage
viable,
acrosome
intact
sperm,
viable
with
active
mitochondria.
Nevertheless,
matured
cumulus-oocyte-complexes
fertilized
which
had
been
preincubated
(MT-Sperm)
showed
higher
(
P
<
0.01)
rates
monospermic
fertilization,
0.05)
polyspermy
enhanced
compared
Control
group.
Moreover,
relative
abundance
MAPK13
-derived
blastocysts
greater
than
observed
In
conclusion,
adding
sperm-preparation
protocol
for
IVF
improved
proper
fertilization
embryonic
.
Journal of Reproduction and Development,
Journal Year:
2017,
Volume and Issue:
64(1), P. 33 - 39
Published: Oct. 27, 2017
This
study
aimed
to
identify
motile
sperm
subpopulations
in
extended
boar
semen
and
observe
the
presumptive
seasonal
variation
their
distribution.
Data
from
4837
ejaculates
collected
over
a
two-year
period
were
analyzed
terms
of
kinematic
parameters
by
Computer
Assisted
Sperm
Analysis
(CASA).
Individual
data
used
determine
subgroups
within
using
cluster
analysis.
Four
(SP)
identified,
with
distinct
movement
patterns:
SP1
high
velocity
linearity;
SP2
but
low
SP3
SP4
linearity.
constituted
least
overall
proportion
(P
<
0.05).
Season
collection
significantly
influenced
different
proportions
subpopulations.
Spring
was
characterized
similar
(NS)
higher
SP3.
Summer
brought
decrease
both
containing
fast
(SP1
SP2)
During
autumn,
increases
recorded.
Winter
substantially
affected
all
0.05)
became
most
represented
subgroup,
while
(fast
linear)
reached
its
highest
compared
other
seasons.
In
conclusion,
is
structured
whose
vary
according
season
collection.
autumn
seem
have
negative
impact
on
linear
subpopulation.
Cluster
analysis
can
be
useful
revealing
differences
quality
that
are
not
normally
detected
classical
evaluation
based
mean
values.
International Journal of Molecular Sciences,
Journal Year:
2022,
Volume and Issue:
23(14), P. 7936 - 7936
Published: July 19, 2022
An
advanced
understanding
of
sperm
function
is
relevant
for
evidence-based
male
fertility
prediction
and
addressing
infertility.
A
standard
breeding
soundness
evaluation
(BSE)
merely
identifies
gross
abnormalities
in
bulls,
whereas
selection
based
on
single
nucleotide
polymorphisms
genomic
estimated
values
overlooks
sub-microscopic
differences
sperm.
Molecular
tools
are
important
validating
advancing
knowledge
the
regulation
at
an
interdisciplinary
level.
Therefore,
research
this
field
now
focused
developing
a
combination
vitro
tests
identifying
biomarkers
such
as
proteins
with
critical
roles
fertility.
The
Na+-K+
ATPase
ubiquitous
transmembrane
protein
its
α4
isoform
(ATP1A4)
exclusively
expressed
germ
cells
Furthermore,
ATP1A4
essential
fertility,
it
interacts
signaling
molecules
both
raft
non-raft
fractions
plasma
membrane
to
regulate
capacitation-associated
signaling,
hyperactivation,
sperm-oocyte
interactions,
activation.
Interestingly,
activity
expression
increase
during
capacitation,
challenging
widely
accepted
dogma
translational
quiescence.
This
review
discusses
literature
role
capacitation
fertilization
events
prospective
use
improving
prediction.
