Veterinary Sciences,
Journal Year:
2024,
Volume and Issue:
11(7), P. 326 - 326
Published: July 18, 2024
The
aims
of
this
study
were
to
characterize
the
semen
as
well
influence
breed,
season,
and
processing
on
spermatozoa
(SPZ)
traits
four
native
Portuguese
goat
breeds
used
for
bank
animal
germplasm
(BPAG).
A
total
1017
ejaculates
from
Serrana
(n
=
30),
Bravia
15),
Charnequeira
11),
Preta
de
Montezinho
3)
bucks
collected
between
2004
2020
at
(EZN-INIAV;
39°
N)
during
whole
year
under
natural
conditions.
All
fresh
cryopreserved
(−196
°C)
was
evaluated
stored
in
BPAG.
(the
smallest
breed)
produced
less
(p
<
0.05)
volume
ejaculate
than
all
other
breeds,
which
higher
full
breeding
season
(September–January;
p
0.05),
regarding
breeds.
Contrarily,
general,
SPZ
concentration
lower
September–January,
but
per
remained
similar
>
May–August
September–January
bucks.
viability
midpiece
defects
slightly
influenced
by
breed
head
(lowest
%
February–April;
0.05).
On
contrary,
freezing–thawing
cycle
strongly
0.01)
traits.
correlation
coefficients
these
thawed
low
(up
0.33;
0.01),
highlighting
importance
cryopreservation.
We
conclude
that
had
a
relevant
effect
traits,
it
much
evident
studied
These
goats
can
serve
donors
throughout
year,
Andrologia,
Journal Year:
2018,
Volume and Issue:
51(4), P. e13223 - e13223
Published: Dec. 27, 2018
The
evaluation
of
sperm
motion
is
crucial
for
processing
seminal
doses
artificial
insemination.
Here,
the
combined
effect
type
and
capture
area
three
counting
chambers,
together
with
diluent
employed,
on
motility
was
analysed.
Ejaculates
from
thirteen
Holstein
bulls
were
used
kinematic
analysis
ISAS®v1
CASA-Mot
system,
using
two
capillary-loaded
chambers
(Leja®
Cell-Vu®)
one
drop
displacement
chamber
(Makler®).
Nine
fixed
positions
analysed
per
type,
considering
central
lateral
longitudinal
fields.
Independent
used,
differences
found
between
chambers.
extender,
no
in
x-axis
observed
Cell-Vu®,
while
Leja®,
some
parameters
showed
lower
values
centre
than
areas.
In
both
lowest
distal
area.
Results
obtained
diluents
highly
different
a
very
low
correlation
them.
conclusion,
inside
leads
to
significant
changes
dilution
media
introduce
considerable
patterns.
It
necessary
optimise
sampling
methods
specific
set-ups
be
technology.
Sel skokhozyaistvennaya Biologiya,
Journal Year:
2017,
Volume and Issue:
52(2), P. 232 - 241
Published: April 1, 2017
Semen
analysis
constitutes
the
base
for
establishment
of
fertility
a
male.In
addition,
number
insemination
doses
to
produce
from
an
ejaculate
depends
on
defined
sperm
characteristics.As
soon
as
image
techniques
were
applied
biological
problems
andrology
was
one
first
fields
benefited
both
in
evaluation
genital
track
and
function.The
improvement
expands
possibilities
using
developed
systems
medical
practice
animal
husbandry.In
ISAS
®
PBos
(«PROISER
-Projectes
i
Serveis
R+D
S.L.»,
Spain)
semen
done
includes
concentration,
motility
morphology.The
with
optimal
rate
frame
capture
transmission
at
images
processing
are
twodimensional
spermatozoa
different
species
regard
sizes.ISAS
calculates
percentage
morpho-abnormalities
analysing
presence
cytoplasmic
droplets
coiled
tails
used
analysis,
allows
calculation
particular
ejaculate.Breed-dependent
morphological
diversity
found
species,
disclosure
structured
subpolations
ejaculate,
lead
reproductive
biology
next
level
open
new
prospects
breeding.In
study
subpopulation
multivariate
statistics
which
is
based
principal
components
applicable.In
statistical
estimation
mathematical
modeling,
it
proposed
use
Bayesian
approach,
basis
toolkit
estimating
quality
will
be
near
future.Essentially,
shortcomings
early
methods
due
modifications
real
germ
cells
counting
chambers
shape
size
under
dehydration,
fixation,
staining
mounting.ISAS
3DTrack
Trumorph
morphology,
respectively,
avoid
limitations.ISAS
device,
lensless
laser
microscope,
depth
around
100
μm.Moreover,
correspondent
hologram
three
dimensions
what
also
big
novelty.Trumorph
technique
offers
maximum
projection
making
possible
obtain
high
resolution
definition
wide
range
including
bull.ISAS
3Fun
kit
software
automatic
method
enables
clear
distinction
intact
plasmalemma
acrosome
essential
function.
Agronomía Mesoamericana,
Journal Year:
2018,
Volume and Issue:
29(2), P. 469 - 469
Published: May 1, 2018
Computer-assisted
semen
analysis
(CASA)
is
used
in
animal
reproduction
with
the
objective
of
evaluating
spermquality,
quickly
and
reliably.
Currently,
artificial
insemination
centers
must
have
reliable
systems
thatensure
reliability
reproductive
data.
The
aim
this
paper
was
to
review
computer-assisted
analysisin
livestock
species.
use
CASA
replace
routine
quality
analyzes
that
introduce
biases
leads
different
inaccuracy
degrees.
In
addition,
if
intrinsic
limitations
microscopy
or
sperm
motilitypatterns
are
considered,
it
most
likely
considerable
variability
will
be
introduced
which
an
impact
on
objectivity
estimation.
At
first,
type
technology
inaccessible,
but
they
currently
routinelyin
research
laboratories
for
evaluation
samples
species
zootechnical
interest..Computerized
techniques
able
issue
reports
a
high
number
characteristics,
minimize
subjectivefactor
analysis,
ensure
better
correlation
fertilizing
capacity
spermatozoa
by
relationship
between
motility
functional
competence.
together
multivariatestatistical
demonstrated
existence
kinetic
morphometric
subpopulations
ejaculates
ofdifferent
species,
eliminating
idea
homogeneous,
formed
single
population
normal
distribution.
Veterinary Sciences,
Journal Year:
2024,
Volume and Issue:
11(7), P. 326 - 326
Published: July 18, 2024
The
aims
of
this
study
were
to
characterize
the
semen
as
well
influence
breed,
season,
and
processing
on
spermatozoa
(SPZ)
traits
four
native
Portuguese
goat
breeds
used
for
bank
animal
germplasm
(BPAG).
A
total
1017
ejaculates
from
Serrana
(n
=
30),
Bravia
15),
Charnequeira
11),
Preta
de
Montezinho
3)
bucks
collected
between
2004
2020
at
(EZN-INIAV;
39°
N)
during
whole
year
under
natural
conditions.
All
fresh
cryopreserved
(−196
°C)
was
evaluated
stored
in
BPAG.
(the
smallest
breed)
produced
less
(p
<
0.05)
volume
ejaculate
than
all
other
breeds,
which
higher
full
breeding
season
(September–January;
p
0.05),
regarding
breeds.
Contrarily,
general,
SPZ
concentration
lower
September–January,
but
per
remained
similar
>
May–August
September–January
bucks.
viability
midpiece
defects
slightly
influenced
by
breed
head
(lowest
%
February–April;
0.05).
On
contrary,
freezing–thawing
cycle
strongly
0.01)
traits.
correlation
coefficients
these
thawed
low
(up
0.33;
0.01),
highlighting
importance
cryopreservation.
We
conclude
that
had
a
relevant
effect
traits,
it
much
evident
studied
These
goats
can
serve
donors
throughout
year,
