Epibiotic bacterial community composition varies during different developmental stages of Octopus mimus: Study of cultivable representatives and their secondary metabolite production DOI Creative Commons
Martha Hengst,

Stephanie Trench,

Valezka Alcayaga

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(12), P. e0312991 - e0312991

Published: Dec. 30, 2024

Marine microbial communities colonizing the skin of invertebrates constitute primary barrier between host and environment, potentially exerting beneficial, neutral, or detrimental effects on fitness. To evaluate potential contribution epibiotic bacteria to survival early developmental stages Octopus mimus , bacterial isolates were obtained from eggs, paralarvae, adults. Their enzymatic activities determined, antibacterial properties assessed against common marine pathogens. The belonged phyla Proteobacteria, Actinomycetota, Bacteroidota, Bacillota, represented by 21 genera 27 species. Specific taxa associated with each stage, only three species shared among different stages: Bacillus pumilus B . megaterium Shewanella algae which also inhibited growth all assayed Organic extracts M8-1 obtained, UHPLC-MS analysis detected seventeen putative compounds, including two phenolic acids, indole derivatives, twelve oxylipins. Our findings provide novel data cultivable representatives isolated capable synthesizing chemical compounds bioactive properties. These results contribute a better understanding role in this invertebrate during critical life stages.

Language: Английский

Isolation and Characterization of Bacteria Colonizing Acartia tonsa Copepod Eggs and Displaying Antagonist Effects against Vibrio anguillarum, Vibrio alginolyticus and Other Pathogenic Strains DOI Creative Commons
Mahammed Zidour,

Mickaël Chevalier,

Yanath Belguesmia

et al.

Frontiers in Microbiology, Journal Year: 2017, Volume and Issue: 8

Published: Oct. 6, 2017

Copepods represent a major source of food for many aquatic species commercial interest aquaculture such as mysis shrimp and early stages fishes. For the purpose this study, culturable mesophilic bacterial flora colonizing Acartia tonsa copepod eggs was isolated identified. A total 175 isolates were characterized based on their morphological biochemical traits. The majority these (70%) Gram-negative bacteria. Matrix-assisted laser desorption/ionization-time flight-mass spectrometry (MALDI-TOF-MS) used rapid identification isolates. Here, 58% successfully identified at genus level among them, 54% level. These belong to 12 different genera 29 species. Five strains, Bacillus pumilus, named 18 COPS, 35A 35R 38 40A showed strong antagonisms against several potential fish pathogens including Vibrio alginolyticus, V. anguillarum, Listeria monocytogenes, Staphylococcus aureus. Furthermore, using differential approach, we show that antimicrobial activity COPS strain is linked primarily production compounds amicoumacin family, demonstrated by specific UV-absorbance MS/MS fragmentation patterns compounds.

Language: Английский

Citations

33
Bacillus and Virgibacillus strains isolated from three Mexican coasts antagonize Staphylococcus aureus and Vibrio parahaemolyticus DOI Open Access
Lucio Galavíz-Silva,

Jesús Mario Iracheta-Villarreal,

Zinnia Judith Molina‐Garza

et al.

FEMS Microbiology Letters, Journal Year: 2018, Volume and Issue: 365(19)

Published: Aug. 16, 2018

This study identified marine microorganisms from Mexican coasts that had antimicrobial activity against Staphylococcus aureus and Vibrio parahaemolyticus, which are known worldwide to be food-poisoning agents. Representative specimens of algae, saline sediment, crustaceans mollusks were collected. Of the 42 tested strains, 15 inhibited these pathogens. Bacillus Virgibacillus strains by 16S rRNA gene sequencing. The with highest inhibitory S. PCRS1-07 (B. aerius), BLCG-05 GUO-01 pumilus). GUHC-04, BLCG-05, GUHC-03 altitudinis) BLBSe-05 oryzicola) showed higher V. parahaemolyticus. Biofilm production all was moderate, but B. altitudinis produced a stronger biofilm. is first isolate aerius, oryzicola, safensis, boroniphilus, senegalensis ecosystems in Mexico as well report their effects both Bioactivity spent media antagonistic cultured biofilm also demonstrated high activity. active compounds antagonists currently being studied tested. Marine have bacterial diversity associated invertebrates seaweed; however, this has not been well-studied on coasts.

Language: Английский

Citations

25
Secondary Metabolites and PI3K Inhibitory Activity of Colletotrichum gloeosporioides, a Fungal Endophyte of Uncaria rhynchophylla DOI

Zhong‐Duo Yang,

Zhijie Li, Junwen Zhao

et al.

Current Microbiology, Journal Year: 2019, Volume and Issue: 76(7), P. 904 - 908

Published: May 18, 2019

Language: Английский

Citations

25
IN VITRO ANTIBACTERIAL ACTIVITIES OF MARINE SPONGE-ASSOCIATED BACTERIA AGAINST PATHOGENIC VIBRIO SPP. CAUSES VIBRIOSIS IN SHRIMPS DOI Open Access
Aris Tri Wahyudi, Jepri Agung Priyanto,

Dian Retno Wulandari

et al.

International Journal of Pharmacy and Pharmaceutical Sciences, Journal Year: 2019, Volume and Issue: unknown, P. 33 - 37

Published: Nov. 1, 2019

Objective: This study was aimed to isolate and screen marine sponge-associated bacteria producing anti-Vibrio compounds identify their from the bacterial extract. Methods: Sponge-associated were isolated by spread plate method. Their activity against Vibrio parahaemolyticus, V. harveyi, vulnificus determined dual culture test. Three potential isolates identified based on 16S-rRNA gene analysis. All tested for haemolytic characters in blood agar medium. Anti-Vibrio of most also using its supernatant, extract, concentrated culture. Chemical composition crude extract derived that GC-MS analysis. Results: 68 have been sponge, Spongia sp., Svenzea Ircinia Igernella sp. Of isolates, 15 (22%) had activities various spectra three species, including vulnificus. non-haemolytic. Bacterial coded as D6.6, D6.19, P4.17 broad spectra. They could inhibit at least two species indicated clear zone formed around colonies. Based 16S-rRNA, these closely related (similarity ≥ 99%) Brevibacterium casei strain M Sw oHS, Bacillus altitudinis FJAT 47750, PgBe190, respectively. D6.6 isolate, which species. Consistently, confirmed culture, isolate. The this contained 10 major are biologically active. Conclusion: suggests strains sponges potentially Vibrio’s growth vitro. These be further explored agent.

Language: Английский

Citations

5
Oleic Acid and Diketopiperazines Produced by Marine Bacteria Reduce the Load of the Pathogen Vibrio parahaemolyticus in Argopecten purpuratus DOI Open Access

Yanett Leyton Carlos Riquelme

Journal of Aquaculture Research & Development, Journal Year: 2013, Volume and Issue: 04(04)

Published: Jan. 1, 2013

The aquaculture industry must often deal in its cultures with bacterial contamination by Vibrio parahaemolyticus, which causes gastroenteritis humans when they eat contaminated organisms.Until recently these pathogens were treated antibiotics are now forbidden because of their negative effects on and the ecosystem.In last few years there have been attempts to solve this problem searching for active metabolites from antagonist bacteria.The objetive work was evaluate decrease load pathogen V. parahaemolyticus Argopecten purpuratus scallops addition oleic acid diketopiperazines isolated marine bacteria known having an antibacterial effect against pathogen, commercially available products similar molecular structures determined most probable number method absence samples gene tdh that codes thermostable direct hemolysin (TDH), main virulence factor species.The A. bivalves showed preliminarily cause a reduction pathogen.The same trend seen commercial diketopiperazines.Based inhibiting activity we suggest possibility experimenting or other different important organisms, mainly depuration systems require short time (12 24 hours) reduce concentration human like parahaemolyticus.

Language: Английский

Citations

3
Epibiotic bacterial community composition varies during different developmental stages of Octopus mimus: Study of cultivable representatives and their secondary metabolite production DOI Creative Commons
Martha Hengst,

Stephanie Trench,

Valezka Alcayaga

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(12), P. e0312991 - e0312991

Published: Dec. 30, 2024

Marine microbial communities colonizing the skin of invertebrates constitute primary barrier between host and environment, potentially exerting beneficial, neutral, or detrimental effects on fitness. To evaluate potential contribution epibiotic bacteria to survival early developmental stages Octopus mimus , bacterial isolates were obtained from eggs, paralarvae, adults. Their enzymatic activities determined, antibacterial properties assessed against common marine pathogens. The belonged phyla Proteobacteria, Actinomycetota, Bacteroidota, Bacillota, represented by 21 genera 27 species. Specific taxa associated with each stage, only three species shared among different stages: Bacillus pumilus B . megaterium Shewanella algae which also inhibited growth all assayed Organic extracts M8-1 obtained, UHPLC-MS analysis detected seventeen putative compounds, including two phenolic acids, indole derivatives, twelve oxylipins. Our findings provide novel data cultivable representatives isolated capable synthesizing chemical compounds bioactive properties. These results contribute a better understanding role in this invertebrate during critical life stages.

Language: Английский

Citations

0