DNA protection, molecular docking, antioxidant, antibacterial, enzyme inhibition, and enzyme kinetic studies for parietin, isolated from Xanthoria parietina (L.) Th. Fr.

Journal of Biomolecular Structure and Dynamics, Journal Year: 2023, Volume and Issue: 42(2), P. 848 - 862

Published: April 6, 2023

Parietin was isolated from Xanthoria parietina (L.) Th. Fr.' (methanol:chloroform) extract, using a silica column. 13 C NMR and 1H were used to confirm the structure of parietin. For first time, parietin investigated for its antioxidant, antibacterial DNA protective activities. Molecular docking carried out determine binding affinity interactions between enzymes our molecule. Inhibition kinetic mechanism studies action performed too. exhibited high metal chelating activity. The MIC values sufficient inhibit different bacterial strains; E. coli, P. aeruginosa, K. pneumoniae S. aureus. applications that acetylcholinesterase (AChE), butyrylcholinesterase (BChE), lipase, tyrosinase have potential with Especially, parietin's highest recorded AChE tyrosinase. These results confirmed by inhibition kinetics results, where, observed potent an IC50 0.013-0.003 µM. Moreover, acts' as non-competitive inhibitor against AChE, BChE, competitive rate stability. promising biological properties revealed effectiveness in terms suitability food pharmaceutical industries.

Language: Английский

Isolation, Chemical Characterization, and Antimicrobial Activity of Secondary Metabolites from Pseudocyphellaria faveolata

Molecules, Journal Year: 2025, Volume and Issue: 30(6), P. 1368 - 1368

Published: March 18, 2025

Introduction: Antimicrobial resistance is a global threat, highlighting the urgent need for novel antimicrobial agents. Among mechanisms of resistance, bacteria can release drug-degrading enzymes and express efflux pumps, as well grow in protected aggregates known biofilms. Pseudomonas aeruginosa Staphylococcus aureus are among most prevalent biofilm infections chronic wounds, respiratory urinary tract infections, device-associated infections. Pseudocyphellaria faveolata (Delise) Malme lichen with metabolites unexplored potential. Aims: To identify characterize major present Faveolata to determine their activity against aeruginosa. Methods: The molecules were purified by column chromatography characterized NMR spectroscopy. compounds was determined terms proliferation, adhesion, viability P. S. broth microdilution method crystal violet staining. Viability resazurin reduction assay on normal human fibroblasts cytotoxicity over cells. Results: spectroscopically identified physciosporin methyl virensate. Physciosporin showed aureus, MIC 32 μg/mL MBC 128 μg/mL, prevented formation from 16 μg/mL. Methyl virensate also had (MIC = 64 μg/mL). None these significantly affected viability, or adhesion. Cytotoxicity at ug/mL below 20%. Conclusions: This first report study compounds. promising preventing biofilms, which responsible These findings provide foundation exploring potential other lichenic depsidones.

Language: Английский

Antimicrobial Activities of Lichens

Published: June 7, 2024

This chapter aims at a comprehensive exploration of lichens, spanning their historical and contemporary applications in the realms medicine agriculture. It delves deeply into intricate chemistry underpinning antimicrobial properties, shedding light on current research endeavors aimed unlocking latent potential inherent these often-overlooked organisms. The unfolding narrative reveals compelling confluence ancient knowledge scientific pursuits, all geared toward perpetual struggle against microbial adversaries. Lichens emerge as prominent reservoir secondary metabolites, prominently displaying wide spectrum activities. Notably, various lichen species including Alectoria sarmentosa , Bryoria fuscescens Cetraria pinastri Cladonia digitata fimbriate Evernia divaricata Lecanora frustulosa Ochrolechia parella Parmeliopsis hyperopta Platismatia glauca Ramalina farinacea have exhibited substantial combatting diverse microorganisms. Their relevance extends from medicinal practices to investigations, reinforcing lichens promising frontier development novel agents within persistent endeavor counteract infections.

Language: Английский