CRISPR/Cas9
has
emerged
as
the
predominant
method
for
genome
editing
due
to
its
cost-effectiveness
and
broad
applicability,
playing
a
crucial
role
in
advancing
sustainable
practices
across
various
sectors.
This
systematic
review
leverages
PRISMA
methodology
explore
CRISPR/Cas9's
impact
on
environmental
protection,
thereby
supporting
Sustainable
Development
Goals
(SDGs).
Analyzing
data
from
Web
of
Science,
found
significant
growth
related
publications,
with
30%
increase
since
2014,
predominantly
US,
China,
Germany,
UK.
The
study
categorizes
scientific
developments
into
three
trends:
advancements
agriculture,
gene
techniques,
biofuel
production.
Key
discussions
include
use
developing
fourth-generation
biofuels
biosensors,
well
applications
enhancing
genetic
resilience
controlling
invasive
species.
These
innovations
highlight
potential
promoting
resource
management
energy
generation,
marking
pivotal
contribution
ecological
conservation
sustainability
efforts.
Veterinary Sciences,
Journal Year:
2025,
Volume and Issue:
12(1), P. 67 - 67
Published: Jan. 17, 2025
Stem
cells
are
unique,
undifferentiated
that
have
the
ability
to
both
replicate
themselves
and
develop
into
specialized
cell
types.
This
dual
capability
makes
them
valuable
in
development
of
regenerative
medicine.
Current
stem
research
has
widened
their
application
therapy,
drug
discovery,
reproductive
cloning
animals,
models
for
various
diseases.
Although
there
substantial
studies
revealing
treatment
human
degenerative
diseases
using
cells,
this
is
yet
be
explored
livestock
animals.
Many
species
such
as
mastitis,
laminitis,
neuromuscular
disorders,
autoimmune
diseases,
some
debilitating
not
covered
completely
by
existing
drugs
can
improved
different
types
like
embryonic
adult
induced
pluripotent
cells.
review
mainly
focuses
on
use
disease
In
addition
mentioned,
potential
helpful
wound
healing,
skin
genetic
disorders.
article
explores
from
sources
therapy
also
role
conservation
endangered
well
model
preparation.
Moreover,
future
perspectives
challenges
associated
with
discussed.
Overall,
transformative
impact
sector
comprehensively
studied
which
will
help
researchers
design
work
related
Marine Drugs,
Journal Year:
2025,
Volume and Issue:
23(1), P. 34 - 34
Published: Jan. 10, 2025
The
convergence
of
marine
sciences
and
medical
studies
has
the
potential
for
substantial
advances
in
healthcare.
This
study
uses
bibliometric
topic
modeling
to
map
progression
research
themes
from
2000
2023,
with
an
emphasis
on
interdisciplinary
subject
sciences.
Building
global
publication
output
at
interface
between
using
Hierarchical
Dirichlet
Process,
we
discovered
dominating
topics
during
three
periods,
emphasizing
shifts
focus
development
trends.
Our
data
show
a
significant
rise
output,
indicating
growing
interest
bioresources
applications.
paper
identifies
two
main
areas
active
research,
"natural
product
biochemistry"
"trace
substance
genetics",
both
great
therapeutic
potential.
We
used
social
network
analysis
collaborative
networks
identify
prominent
scholars
institutions
driving
this
progress.
indicates
important
paths
policy
R&D
management
operating
crossroads
healthcare
innovation
It
also
underscores
significance
quantitative
foresight
methods
teams
identifying
interpreting
future
scientific
convergences
breakthroughs.
Molecular Cancer,
Journal Year:
2025,
Volume and Issue:
24(1)
Published: March 21, 2025
Tumor
drug
resistance
presents
a
growing
challenge
in
medical
practice,
particularly
during
anti-cancer
therapies,
where
the
emergence
of
drug-resistant
cancer
cells
significantly
complicates
clinical
treatment.
In
recent
years,
three-dimensional
(3D)
tumor
culture
technology,
which
more
effectively
simulates
vivo
physiological
environment,
has
gained
increasing
attention
research
and
applications.
By
mimicking
cellular
microenvironment,
3D
technology
not
only
recapitulates
cell-cell
interactions
but
also
faithfully
reproduces
biological
effects
therapeutic
agents.
Consequently,
is
emerging
as
crucial
tool
biomedical
research.
We
summarize
benefits
models
organoid
explore
their
application
realm
resistance,
screening,
personalized
therapy,
discuss
potential
prospects
challenges
transformation,
with
aim
providing
insights
for
optimizing
treatment
strategies
advancing
precision
therapy.
Tropical Medicine and Health,
Journal Year:
2025,
Volume and Issue:
53(1)
Published: April 2, 2025
Abstract
Background
Antimicrobial
resistance
(AMR)
poses
a
global
health
threat,
particularly
in
low-
and
middle-income
countries
(LMICs).
Clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)–Cas
system
technology
offers
promising
tool
to
combat
AMR
by
targeting
disabling
genes
WHO
bacterial
priority
pathogens.
Thus,
we
systematically
reviewed
the
potential
of
CRISPR–Cas
address
AMR.
Methods
This
systematic
review
adhered
Preferred
Reporting
Items
for
Systematic
Reviews
Meta-Analyses
(PRISMA)
guidelines.
A
comprehensive
literature
search
was
conducted
using
Scopus
PubMed
databases,
focusing
on
publications
from
2014
June
2024.
Keywords
included
“CRISPR/Cas,”
“antimicrobial
resistance,”
“pathogen.”
The
eligibility
criteria
required
original
studies
involving
CRISPR/Cas
systems
that
targeted
Data
were
extracted
eligible
studies,
qualitatively
synthesized,
assessed
bias
Joanna
Briggs
Institute
(JBI)-standardized
tool.
Results
48
revealed
diverse
systems,
including
CRISPR–Cas9,
CRISPR–Cas12a,
CRISPR–Cas3,
various
genes,
such
as
blaOXA-232,
blaNDM,
blaCTX-M,
ermB,
vanA,
mecA
,
fosA3
blaKPC
mcr-1,
which
are
responsible
carbapenem,
cephalosporin,
methicillin,
macrolide,
vancomycin,
colistin,
fosfomycin
resistance.
Some
have
explored
role
CRISPR
virulence
gene
suppression,
enterotoxin
tsst1
iutA
Staphylococcus
aureus
Klebsiella
pneumoniae
.
Delivery
mechanisms
include
bacteriophages,
nanoparticles,
electro-transformation,
conjugative
plasmids,
demonstrate
high
efficiency
vitro
vivo.
CRISPR-based
diagnostic
applications
demonstrated
sensitivity
specificity,
with
detection
limits
low
2.7
×
10
2
CFU/mL,
significantly
outperforming
conventional
methods.
Experimental
reported
significant
reductions
resistant
populations
complete
suppression
strains.
Engineered
phagemid
particles
plasmid-curing
been
shown
eliminate
IncF
cured
plasmids
carrying
vanA
mcr-1
blaNDM
94%
efficiency,
restore
antibiotic
susceptibility.
Gene
re-sensitization
strategies
used
susceptibility
E.
coli
blaKPC-2-mediated
carbapenem
MDR
bacteria.
Whole-genome
sequencing
bioinformatics
tools
provided
deeper
insights
into
CRISPR-mediated
defense
mechanisms.
Optimization
enhanced
gene-editing
efficiencies,
offering
approach
tackling
high-priority
Conclusions
has
across
While
promising,
challenges
optimizing
vivo
delivery,
mitigating
resistance,
navigating
ethical-regulatory
barriers
must
be
addressed
facilitate
clinical
translation.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(22), P. 12424 - 12424
Published: Nov. 19, 2024
Microbial
plant
biostimulants
offer
a
promising,
sustainable
solution
for
enhancing
growth
and
resilience,
particularly
under
abiotic
stress
conditions
such
as
drought,
salinity,
extreme
temperatures,
heavy
metal
toxicity.
These
biostimulants,
including
growth-promoting
rhizobacteria,
mycorrhizal
fungi,
nitrogen-fixing
bacteria,
enhance
tolerance
through
mechanisms
phytohormone
production,
nutrient
solubilization,
osmotic
adjustment,
antioxidant
enzyme
activation.
Advances
in
genomics,
metagenomics,
transcriptomics,
proteomics
have
significantly
expanded
our
understanding
of
plant-microbe
molecular
communication
the
rhizosphere,
revealing
underlying
these
interactions
that
promote
resilience.
However,
challenges
inconsistent
field
performance,
knowledge
gaps
stress-related
signaling,
regulatory
hurdles
continue
to
limit
broader
biostimulant
adoption.
Despite
challenges,
microbial
hold
significant
potential
advancing
agricultural
sustainability,
amid
climate
change-induced
stresses.
Future
studies
innovation,
Clustered
Regularly
Interspaced
Short
Palindromic
Repeats
other
editing
tools,
should
optimize
formulations
their
application
diverse
agro-ecological
systems.
This
review
aims
underscore
current
advances,
future
directions
field,
advocating
multidisciplinary
approach
fully
harness
modern
agriculture.
Molecules,
Journal Year:
2024,
Volume and Issue:
29(19), P. 4737 - 4737
Published: Oct. 7, 2024
Nucleic
acid
therapeutics
are
promising
alternatives
to
conventional
anti-cancer
therapy,
such
as
chemotherapy
and
radiation
therapy.
While
therapies
have
limitations,
high
side
effects,
low
specificity,
drug
resistance,
nucleic
work
at
the
gene
level
eliminate
cause
of
disease.
treat
diseases
in
various
forms
using
different
mechanisms,
including
plasmid
DNA
(pDNA),
small
interfering
RNA
(siRNA),
anti-microRNA
(anti-miR),
microRNA
mimics
(miRNA
mimic),
messenger
(mRNA),
aptamer,
catalytic
(CNA),
CRISPR
cas9
guide
(gRNA).
In
addition,
acids
many
advantages
nanomaterials,
biocompatibility,
design
flexibility,
immunogenicity,
size,
relatively
price,
easy
functionalization.
can
a
therapeutic
effect
by
being
used
combination
with
nanostructures,
inorganic
nanoparticles,
lipid
nanoparticles
(LNPs),
etc.
overcome
physiological
stability
cell
internalization
efficiency.
The
field
has
advanced
remarkably
recent
decades,
more
been
approved,
they
already
demonstrated
their
potential
diseases,
cancer.
This
review
paper
introduces
current
status
advances
therapy
for
treatment
discusses
tasks
prospects
ahead.