Stem Cell Therapy for Diseases of Livestock Animals: An In-Depth Review

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(1), P. 67 - 67

Published: Jan. 17, 2025

Stem cells are unique, undifferentiated that have the ability to both replicate themselves and develop into specialized cell types. This dual capability makes them valuable in development of regenerative medicine. Current stem research has widened their application therapy, drug discovery, reproductive cloning animals, models for various diseases. Although there substantial studies revealing treatment human degenerative diseases using cells, this is yet be explored livestock animals. Many species such as mastitis, laminitis, neuromuscular disorders, autoimmune diseases, some debilitating not covered completely by existing drugs can improved different types like embryonic adult induced pluripotent cells. review mainly focuses on use disease In addition mentioned, potential helpful wound healing, skin genetic disorders. article explores from sources therapy also role conservation endangered well model preparation. Moreover, future perspectives challenges associated with discussed. Overall, transformative impact sector comprehensively studied which will help researchers design work related

Language: Английский

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Investigating Past, Present, and Future Trends on Interface Between Marine and Medical Research and Development: A Bibliometric Review

Marine Drugs, Journal Year: 2025, Volume and Issue: 23(1), P. 34 - 34

Published: Jan. 10, 2025

The convergence of marine sciences and medical studies has the potential for substantial advances in healthcare. This study uses bibliometric topic modeling to map progression research themes from 2000 2023, with an emphasis on interdisciplinary subject sciences. Building global publication output at interface between using Hierarchical Dirichlet Process, we discovered dominating topics during three periods, emphasizing shifts focus development trends. Our data show a significant rise output, indicating growing interest bioresources applications. paper identifies two main areas active research, "natural product biochemistry" "trace substance genetics", both great therapeutic potential. We used social network analysis collaborative networks identify prominent scholars institutions driving this progress. indicates important paths policy R&D management operating crossroads healthcare innovation It also underscores significance quantitative foresight methods teams identifying interpreting future scientific convergences breakthroughs.

Language: Английский

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Advances in environmental biotechnology with CRISPR/Cas9: bibliometric review and cutting-edge applications

Deleted Journal, Journal Year: 2025, Volume and Issue: 7(3)

Published: Feb. 22, 2025

Language: Английский

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Applications of Gene Editing and Nanotechnology in Stem Cell-Based Therapies for Human Diseases

Stem Cell Reviews and Reports, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

Language: Английский

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3D tumor cultures for drug resistance and screening development in clinical applications

Molecular Cancer, Journal Year: 2025, Volume and Issue: 24(1)

Published: March 21, 2025

Tumor drug resistance presents a growing challenge in medical practice, particularly during anti-cancer therapies, where the emergence of drug-resistant cancer cells significantly complicates clinical treatment. In recent years, three-dimensional (3D) tumor culture technology, which more effectively simulates vivo physiological environment, has gained increasing attention research and applications. By mimicking cellular microenvironment, 3D technology not only recapitulates cell-cell interactions but also faithfully reproduces biological effects therapeutic agents. Consequently, is emerging as crucial tool biomedical research. We summarize benefits models organoid explore their application realm resistance, screening, personalized therapy, discuss potential prospects challenges transformation, with aim providing insights for optimizing treatment strategies advancing precision therapy.

Language: Английский

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Unveiling the future of cancer stem cell therapy: a narrative exploration of emerging innovations

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 22, 2025

Cancer stem cells (CSCs), are a critical subpopulation within tumours, and defined by their capacity for self-renewal, differentiation, tumour initiation. These unique traits contribute to progression, metastasis, resistance conventional treatments like chemotherapy radiotherapy, often resulting in cancer recurrence poor patient outcomes. As such, CSCs have become focal points developing advanced therapies. This review highlights progress CSC-targeted treatments, including chimeric antigen receptor T-cell (CAR-T) therapy, immunotherapy, molecular targeting, nanoparticle-based drug delivery systems. Plant-derived compounds gene-editing technologies, such as clustered regularly interspaced short palindromic repeats (CRISPR), explored potential enhance precision minimize side effects. Metabolic pathways integral CSC survival, mitochondrial dynamics, mitophagy (regulated dynamin-related protein 1 [DRP1] the PINK1/Parkin pathway), one-carbon metabolism, amino acid metabolism (involving enzymes glutaminase (GLS) glutamate dehydrogenase (GDH]), lipid hypoxia-induced metabolic reprogramming mediated hypoxia-inducible factors (HIF-1α HIF-2α), examined therapeutic targets. The adaptability of through autophagy, flexibility, epigenetic regulation metabolites α-ketoglutarate, succinate, fumarate is discussed. Additionally, extracellular vesicles nicotinamide adenine dinucleotide (NAD⁺) identified pivotal redox balance, DNA repair, modifications. Addressing challenges heterogeneity, immune evasion, treatment durability requires interdisciplinary collaboration. Advancing therapies essential overcoming preventing relapse, paving way transformative treatments. underscores importance leveraging innovative technologies fostering collaboration revolutionize treatment.

Language: Английский

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Reinvigorating AMR resilience: leveraging CRISPR–Cas technology potentials to combat the 2024 WHO bacterial priority pathogens for enhanced global health security—a systematic review

Tropical Medicine and Health, Journal Year: 2025, Volume and Issue: 53(1)

Published: April 2, 2025

Abstract Background Antimicrobial resistance (AMR) poses a global health threat, particularly in low- and middle-income countries (LMICs). Clustered regularly interspaced short palindromic repeats (CRISPR)–Cas system technology offers promising tool to combat AMR by targeting disabling genes WHO bacterial priority pathogens. Thus, we systematically reviewed the potential of CRISPR–Cas address AMR. Methods This systematic review adhered Preferred Reporting Items for Systematic Reviews Meta-Analyses (PRISMA) guidelines. A comprehensive literature search was conducted using Scopus PubMed databases, focusing on publications from 2014 June 2024. Keywords included “CRISPR/Cas,” “antimicrobial resistance,” “pathogen.” The eligibility criteria required original studies involving CRISPR/Cas systems that targeted Data were extracted eligible studies, qualitatively synthesized, assessed bias Joanna Briggs Institute (JBI)-standardized tool. Results 48 revealed diverse systems, including CRISPR–Cas9, CRISPR–Cas12a, CRISPR–Cas3, various genes, such as blaOXA-232, blaNDM, blaCTX-M, ermB, vanA, mecA , fosA3 blaKPC mcr-1, which are responsible carbapenem, cephalosporin, methicillin, macrolide, vancomycin, colistin, fosfomycin resistance. Some have explored role CRISPR virulence gene suppression, enterotoxin tsst1 iutA Staphylococcus aureus Klebsiella pneumoniae . Delivery mechanisms include bacteriophages, nanoparticles, electro-transformation, conjugative plasmids, demonstrate high efficiency vitro vivo. CRISPR-based diagnostic applications demonstrated sensitivity specificity, with detection limits low 2.7 × 10 2 CFU/mL, significantly outperforming conventional methods. Experimental reported significant reductions resistant populations complete suppression strains. Engineered phagemid particles plasmid-curing been shown eliminate IncF cured plasmids carrying vanA mcr-1 blaNDM 94% efficiency, restore antibiotic susceptibility. Gene re-sensitization strategies used susceptibility E. coli blaKPC-2-mediated carbapenem MDR bacteria. Whole-genome sequencing bioinformatics tools provided deeper insights into CRISPR-mediated defense mechanisms. Optimization enhanced gene-editing efficiencies, offering approach tackling high-priority Conclusions has across While promising, challenges optimizing vivo delivery, mitigating resistance, navigating ethical-regulatory barriers must be addressed facilitate clinical translation.

Language: Английский

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Molecular Communication of Microbial Plant Biostimulants in the Rhizosphere Under Abiotic Stress Conditions

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 12424 - 12424

Published: Nov. 19, 2024

Microbial plant biostimulants offer a promising, sustainable solution for enhancing growth and resilience, particularly under abiotic stress conditions such as drought, salinity, extreme temperatures, heavy metal toxicity. These biostimulants, including growth-promoting rhizobacteria, mycorrhizal fungi, nitrogen-fixing bacteria, enhance tolerance through mechanisms phytohormone production, nutrient solubilization, osmotic adjustment, antioxidant enzyme activation. Advances in genomics, metagenomics, transcriptomics, proteomics have significantly expanded our understanding of plant-microbe molecular communication the rhizosphere, revealing underlying these interactions that promote resilience. However, challenges inconsistent field performance, knowledge gaps stress-related signaling, regulatory hurdles continue to limit broader biostimulant adoption. Despite challenges, microbial hold significant potential advancing agricultural sustainability, amid climate change-induced stresses. Future studies innovation, Clustered Regularly Interspaced Short Palindromic Repeats other editing tools, should optimize formulations their application diverse agro-ecological systems. This review aims underscore current advances, future directions field, advocating multidisciplinary approach fully harness modern agriculture.

Language: Английский

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Recent Advances and Prospects of Nucleic Acid Therapeutics for Anti-Cancer Therapy

Molecules, Journal Year: 2024, Volume and Issue: 29(19), P. 4737 - 4737

Published: Oct. 7, 2024

Nucleic acid therapeutics are promising alternatives to conventional anti-cancer therapy, such as chemotherapy and radiation therapy. While therapies have limitations, high side effects, low specificity, drug resistance, nucleic work at the gene level eliminate cause of disease. treat diseases in various forms using different mechanisms, including plasmid DNA (pDNA), small interfering RNA (siRNA), anti-microRNA (anti-miR), microRNA mimics (miRNA mimic), messenger (mRNA), aptamer, catalytic (CNA), CRISPR cas9 guide (gRNA). In addition, acids many advantages nanomaterials, biocompatibility, design flexibility, immunogenicity, size, relatively price, easy functionalization. can a therapeutic effect by being used combination with nanostructures, inorganic nanoparticles, lipid nanoparticles (LNPs), etc. overcome physiological stability cell internalization efficiency. The field has advanced remarkably recent decades, more been approved, they already demonstrated their potential diseases, cancer. This review paper introduces current status advances therapy for treatment discusses tasks prospects ahead.

Language: Английский

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Advancements in pathology: Digital transformation, precision medicine, and beyond

Journal of Pathology Informatics, Journal Year: 2024, Volume and Issue: 16, P. 100408 - 100408

Published: Nov. 19, 2024

Pathology, a cornerstone of medical diagnostics and research, is undergoing revolutionary transformation fueled by digital technology, molecular biology advancements, big data analytics. Digital pathology converts conventional glass slides into high-resolution images, enhancing collaboration efficiency among pathologists worldwide. Integrating artificial intelligence (AI) machine learning (ML) algorithms with improves diagnostic accuracy, particularly in complex diseases like cancer. Molecular pathology, facilitated next-generation sequencing (NGS), provides comprehensive genomic, transcriptomic, proteomic insights disease mechanisms, guiding personalized therapies. Immunohistochemistry (IHC) plays pivotal role biomarker discovery, refining classification prognostication. Precision medicine integrates pathology's findings individual genetic, environmental, lifestyle factors to customize treatment strategies, optimizing patient outcomes. Telepathology extends services underserved areas through remote pathology. Pathomics leverages analytics extract meaningful from advancing our understanding therapeutic targets. Virtual autopsies employ non-invasive imaging technologies revolutionize forensic These innovations promise earlier diagnoses, tailored treatments, enhanced care. Collaboration across disciplines essential fully realize the transformative potential these advancements practice research.

Language: Английский

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