On the trail of pulmonary tuberculosis based on rib lesions: Results from the human identified skeletal collection from the Museu Bocage (Lisbon, Portugal)

American Journal of Physical Anthropology, Journal Year: 2005, Volume and Issue: 130(2), P. 190 - 200

Published: Dec. 19, 2005

Abstract In the last 20 years, studies on human identified skeletal collections have revealed a significant relationship between new bone formation visceral surface of ribs and pulmonary tuberculosis (TB). To improve methods differential diagnosis respiratory diseases in archaeological skeletons, an investigation was conducted 197 individuals from Human Identified Skeletal Collection Museu Bocage (Lisbon, Portugal). This sample included 109 males 88 females who lived during 19th–20th centuries, with ages at death ranging 13–88 years. The skeletons were grouped according to cause death: 1) TB (N = 84); 2) non‐TB 49); 3) control group 64) composed randomly selected among extrapulmonary causes death. ribs, sterna, scapulae, clavicles macroscopically observed. New recorded 90.5% (76/84) died TB, 36.7% (18/49) disease as death, 25.0% (16/64) group. These differences statistically ( P < 0.001). Furthermore, bony lesions presented mainly lamellar vertebral end upper middle thoracic rib cage. Proliferative alterations also occurred one sternum nine eight scapulae. work strongly supports results similar performed other documented collections, suggesting that although not pathognomonic, is useful criterion for TB. Am J Phys Anthropol, 2006. © 2005 Wiley‐Liss, Inc.

Language: Английский

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Molecular evidence for different stages of tuberculosis in ancient bone samples from Hungary

American Journal of Physical Anthropology, Journal Year: 2000, Volume and Issue: 113(3), P. 293 - 304

Published: Jan. 1, 2000

This paleomicrobiologic study was conducted on osseous tissue specimens from ancient Hungarian skeletal samples the 7–8th and 17th centuries AD with typical macromorphologic evidence of tuberculosis (n = 3), morphologic alterations probably due to 6), or nontypical changes vertebral bodies suggestive inflammatory reaction 5). From these bone samples, DNA extracted amplified by polymerase chain (PCR) using various primer pairs recognizing segments different mycobacterial species. To confirm specificity analysis, amplification products several were subjected restriction enzyme digestion and/or direct sequencing. Of analyzed 14 cases, 8 unambiguously positive for Mycobacterium complex, as shown IS6110 sequence. In 13 cases we found a PCR product primers specific 65-kDa antigen gene, including 2 without genomic DNA. We conclude that application other may reveal contamination bones atypical saprophytic mycobacteria. A result mycobacteria seen in 3 signs amplifiable DNA, 6 probable but also changes. indicates minor reactions surface be due—at least cases—to infections bacteria M. complex. disease have proceeded rapidly, represent "early" stages tuberculous infection vertebrae. Am J Phys Anthropol 113:293–304, 2000. © 2000 Wiley-Liss, Inc.

Language: Английский

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Using ancient DNA analysis in palaeopathology: a critical analysis of published papers, with recommendations for future work

International Journal of Osteoarchaeology, Journal Year: 2008, Volume and Issue: 18(6), P. 600 - 613

Published: May 2, 2008

Abstract Despite an observable increase in the number of studies using ancient DNA analysis to diagnose disease human remains, there remain issues be addressed about quality resulting publications. This paper describes qualitative published papers that describe detection pathogenic skeletal and mummified remains from archaeological sites. Its ultimate goal is provide overview main problematic relationship standards developed molecular biology make recommendations for future work. Sixty‐five between 1993 2006 were surveyed each was assessed 15 criteria. Interesting results emerged. Of particular note high did not acknowledge use even basic contamination control (90%) or procedures validate independently (85%). study illustrates attention authentication needed research, if confidence aDNA palaeopathology increased. Additionally, methods must described ensure transparency processes utilised generate data. Copyright © 2008 John Wiley & Sons, Ltd.

Language: Английский

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The Categorisation of Occupation in Identified Skeletal Collections: A Source of Bias?

International Journal of Osteoarchaeology, Journal Year: 2012, Volume and Issue: 23(2), P. 186 - 196

Published: Oct. 15, 2012

ABSTRACT Identified skeletal collections, that is, skeletons for which sex, age at death and occupation are known, have been used to test methods recording entheseal changes (EC). By testing on identified the sensitivity of EC activity levels can be ascertained prior applying hypotheses in archaeological contexts. However, definition occupational categories this research may, itself, a source bias. The aim study was how categorising affected interpretation EC. Male ( n = 211) from two Portuguese collections were used. Three occupations, all previously published, each dividing occupations into five, three categories, respectively. Fibrocartilaginous entheses recorded scored as present/absent. Results showed method frequencies found specific entheses. Frequencies significantly different between one not necessarily significant others. This demonstrates categorisation does affect results. using logistic regression, we greater effect than occupation. These results demonstrate need standardised well importance considering age. Copyright © 2012 John Wiley & Sons, Ltd.

Language: Английский

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Investigation of the link between visceral surface rib lesions and tuberculosis in a Medieval skeletal series from England using ancient DNA

American Journal of Physical Anthropology, Journal Year: 2002, Volume and Issue: 119(1), P. 27 - 36

Published: Aug. 14, 2002

Abstract Seven human skeletons from a large assemblage rural English Medieval burial site show lesions, predominantly proliferative in nature, on the visceral surfaces of ribs. In order to investigate whether these rib lesions were regularly associated with tuberculous infection, individuals, together group age‐ and sex‐matched control without bony signs subjected polymerase chain reaction (PCR) assays aimed at detecting traces DNA infecting microorganisms Mycobacterium tuberculosis complex. The results provided no evidence for any regular association between surface presence M. complex study group. significance findings paleopathological interpretation is discussed. Am J Phys Anthropol 119:27–36, 2002. © 2002 Wiley‐Liss, Inc.

Language: Английский

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Leprosy and tuberculosis in Iron Age Southeast Asia?

American Journal of Physical Anthropology, Journal Year: 2004, Volume and Issue: 125(3), P. 239 - 256

Published: Jan. 12, 2004

Abstract The recent excavation of a sample 120 human skeletons from an Iron Age site in the valley Mun River, tributary Mekong River on Khorat Plateau northeast Thailand, has provided largest this period region to date. This paper reviews three individuals with pathological changes for which differential diagnosis includes systemic infectious disease. In two these, both males lesions hands and feet, leprosy psoriatic arthritis are discussed as diagnoses, most probable. third, female spine, tuberculosis nonspecific osteomyelitis. Tuberculosis is probable diagnosis. Although focus presentation evidence disease at Noen U‐Loke, significance diagnoses mycobacterial diseases history prehistory mainland Southeast Asia also briefly discussed. Am J Phys Anthropol, 2004. © 2004 Wiley‐Liss, Inc.

Language: Английский

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Genotyping of ancientMycobacterium tuberculosisstrains reveals historic genetic diversity

Proceedings of the Royal Society B Biological Sciences, Journal Year: 2014, Volume and Issue: 281(1781), P. 20133236 - 20133236

Published: Feb. 26, 2014

The evolutionary history of the Mycobacterium tuberculosis complex (MTBC) has previously been studied by analysis sequence diversity in extant strains, but not addressed direct examination strain genotypes archaeological remains. Here, we use ancient DNA sequencing to type 11 single nucleotide polymorphisms and two large MTBC strains present 10 samples from skeletons Britain Europe dating second-nineteenth centuries AD. results enable us assign groupings lineages recognized MTBC. We show that at least during eighteenth-nineteenth AD, M. belonging different genetic groups were same time, possibly even a location, evidence for mixed infection one individual. Our study shows typing applied multiple can provide sufficiently detailed information contribute both knowledge tuberculosis.

Language: Английский

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Skeletal evidence of tuberculosis in a modern identified human skeletal collection (Certosa cemetery, Bologna, Italy)

American Journal of Physical Anthropology, Journal Year: 2015, Volume and Issue: 157(3), P. 389 - 401

Published: Feb. 24, 2015

The diagnosis of tuberculosis (TB) in osteoarcheological series relies on the identification osseous lesions caused by disease. study identified skeletal collections provides opportunity to investigate distribution relation this aim was examine evidence for TB late adolescent and adult individuals from human collection Certosa cemetery Bologna (Italy, 19th-20th c.). sample group consists 244 (138 males, 106 females) ranging 17 88 years age. divided into three groups basis recorded cause death: (N = 64), pulmonary non-TB 29), other diseases 151). Skeletal reported be related were analyzed. vertebral classified types: enlarged foramina (EnF, vascular with diameter 3-5 mm), erosions (ER), (OtF, cavities various shapes > 3 mm). A CT scan analysis also performed bodies. Some seldom present our (e.g., tuberculous arthritis). OtF (23.7%) subperiosteal new bone formation ribs (54.2%) are significantly more frequent respect groups. showed that vertebrae who have died may internal absence external lesions. These traits represent useful elements paleopathological TB.

Language: Английский

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The association between skeletal lesions and tuberculosis diagnosis using a probabilistic approach

International Journal of Paleopathology, Journal Year: 2019, Volume and Issue: 27, P. 88 - 100

Published: Jan. 18, 2019

Language: Английский

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A Late Neolithic Case of Pott's Disease from Hungary

International Journal of Osteoarchaeology, Journal Year: 2012, Volume and Issue: 24(6), P. 697 - 703

Published: May 28, 2012

Language: Английский

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