Monitoring Biodiversity Impacts of a Changing Arctic Through Environmental DNA
Oceanography,
Journal Year:
2023,
Volume and Issue:
unknown
Published: Jan. 1, 2023
The
Ocean
Molecular
Ecology
(OME)
program
at
the
NOAA
Pacific
Marine
Environmental
Laboratory
(PMEL)
is
a
recently
established
research
group
that
partners
with
several
PMEL
and
Alaska
Fisheries
Science
Center
(AFSC)
programs
including
Ecosystems
&
Fisheries-Oceanography
Coordinated
Investigations
(EcoFOCI),
Carbon,
Earth-Ocean
Interactions
(EOI).
OME
utilizes
suite
of
molecular
tools
to
support
long-term
initiatives
through
genomics
applications,
their
development
application
as
novel
environmental
(e)DNA
approaches
has
been
key
for
facilitating
collaborations
within
Oceanic
Atmospheric
Research,
across
line
offices.
Language: Английский
Evaluating Metabarcoding Markers for Identifying Zooplankton and Ichthyoplankton Communities to Species in the Salish Sea: Morphological Comparisons and Rare, Threatened or Invasive Species
DNA,
Journal Year:
2023,
Volume and Issue:
4(1), P. 1 - 33
Published: Dec. 22, 2023
Zooplankton
and
ichthyoplankton
community
assessments
depend
on
species
diagnostics,
yet
morphological
identifications
are
time-consuming,
require
taxonomic
expertise,
hampered
by
a
lack
of
diagnostic
characters,
particularly
for
larval
stages.
Metabarcoding
can
identify
multiple
in
communities
from
short
DNA
sequences
comparison
to
reference
databases.
To
evaluate
resolution
across
phylogenetic
groups
food
webs
zooplankton
ichthyoplankton,
we
compare
five
metabarcode
mitochondrial
(mt)DNA
markers
gene
regions
(a)
cytochrome
c
oxidase
subunit
I,
(b)
b,
(c)
16S
ribosomal
RNA,
(d)
12S
RNA
extracted
net
tows
the
Northeastern
Pacific
Ocean’s
Salish
Sea
seven
sites
two
seasons.
Species
resolved
metabarcoding
compared
invertebrate
biomass
estimates.
Results
indicate
that
different
taxa
markedly
vary
among
identifications.
Thus,
researchers
seeking
“universal”
should
take
caution
several
likely
will
be
needed;
all
miss
some
yield
incomplete
overlap.
requires
careful
attention
taxon
marker
selection
coverage
sequence
repositories.
In
summary,
combined
multi-marker
approaches
improve
broadscale
diagnostics.
Language: Английский
The History and Evolution of PMEL: Purposeful Research that Impacts Environmental Policy
Oceanography,
Journal Year:
2023,
Volume and Issue:
unknown
Published: Jan. 1, 2023
The
Pacific
Marine
Environmental
Laboratory
(PMEL)
conducts
global
and
regional
oceanographic
research
in
support
of
the
National
Oceanic
Atmospheric
Administration’s
(NOAA’s)
three
mission
areas:
(1)
understanding
predicting
changes
climate,
weather,
oceans,
coasts;
(2)
sharing
that
knowledge
with
others;
(3)
conserving
managing
coastal
marine
ecosystems
resources.
Since
its
establishment
1973,
PMEL’s
work
has
ranged
from
pole
to
across
ocean.
lab’s
examined
ocean
structure
function
physical,
chemical,
biological
realms,
informed
supported
development
US
policy
these
areas.
Language: Английский