Ligustrazine hydrochloride Prevents Ferroptosis by Activating the NRF2 Signaling Pathway in a High-Altitude Cerebral Edema Rat Model

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 1110 - 1110

Published: Jan. 27, 2025

High-altitude cerebral edema (HACE) is a disorder caused by low pressure and hypoxia at high altitudes. Nevertheless, as of now, there still scarcity safe effective prevention treatment methods. The active component Ligusticum Chuanxiong, namely Ligustrazine hydrochloride (LH), has shown potential in the HACE due to its anti-inflammatory, antioxidant, neuroprotective effects nervous system disorders. Consequently, protective effect LH on mechanism need be further explored. Prior modeling, 90 male Sprague-Dawley rats were pretreated with different doses drugs, including (100 mg/kg 50 mg/kg), dexamethasone (4 ML385 (30 mg/kg). Subsequently, placed low-pressure anoxic chamber simulating plateau environment establish rat model. mechanisms elucidated through determination brain water content, HE staining, ELISA, immunofluorescence, molecular docking, dynamics simulation, western blot, other techniques. results showed, first all, that pretreatment can effectively reduce content; down-regulate expression AQP4, HIF-1α, VEGF proteins; alleviate damage tissue nerve cells. Secondly, compared group, significantly MDA levels increase GSH SOD levels. Additionally, decreased inflammatory factors IL-1β, IL-6, TNF-α; reduced total iron content tissue; increased ferroptosis-related proteins such SLC7A11, GPX4, FTH1; alleviated ferroptosis occurrence. Molecular docking simulations show strong binding affinity for NRF2 signaling. Western blot analysis confirmed promotes translocation from cytoplasm nucleus activates signaling pathway exert an antioxidant effect. inhibitor reverse anti-oxidative stress tissue. In summary, may have activating pathway, inhibiting ferroptosis, resisting oxidative stress.

Language: Английский

Top 100 most-cited articles on apoptosis of non-small cell lung cancer over the past two decades: a bibliometrics analysis

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 13, 2025

Background Recently there has been an increasing number of studies have explored apoptosis mechanisms in lung cancer (LC). However, no researchers conducted a bibliometric analysis the most cited articles this field. Objective To examine top 100 influential and publications on non-small cell (NSCLC) from 2004 to 2023, summarizing research trends key focus areas. Methods This study utilized Web Science Core Database (WOSCC) NSCLC using keyword selection manual screening for article searches. Bibliometrix package R software 4.3.1 was used generate distribution statistics ten institutions, journals authors. Citespace6.2. R6 create visualization maps co-occurrence clustering. VOSviewer1.6.19 conduct cluster publishing countries (regions), with data exported SCImago Graphica geographic cooperation analysis. produced co-citation journals, authors, references. Results From 13316 were retrieved, chosen. These authored by 934 individuals 269 institutions across 18 appeared 45 journals. Citations ranged 150 1,389, median 209.5. The 2005 2007 (n=13). leading authors identified as United States (n=60), Harvard University (n=64), CANCER RESEARCH (n=15), SUN M YANG JS (n=6). five keywords “expression”, “activation”, “apoptosis”, “pathway” “gefitinib”. indicates that enhancing through circular RNA regulation targeting Nrf2 signaling pathway could become recent years. Conclusion Apoptosis subject extensive over many years, particularly relation its role pathogenesis, diagnosis, treatment NSCLC. aims identify highly forecast emerging trends, thereby offering insights into novel therapeutic targets strategies overcome drug resistance. findings are intended serve valuable reference scholars engaged field study.

Language: Английский