Neuronal guanine nucleotide exchange factor promotes the axonal growth and cancer cell proliferation via Ephrin-A3/EphA2 axis in lung adenocarcinoma DOI Creative Commons
Jie Mi, Wentian Zhang,

Yijiu Ren

et al.

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 28, 2025

Neural infiltration has been found in various cancers and the infiltrating nerves influence tumor growth dissemination. In non-small cell lung cancer, pan-neuronal marker PGP9.5 was detected by immunohistochemical staining its high expression correlated with poor prognosis. However, existence of nerve fibers mechanism driving neural remains unclear. We first used to assess density patients adenocarcinoma different sizes. Following that, we performed differential analysis univariate Cox prognostic analysis, using public datasets experiments identify gene that triggers is associated cancer progression unfavorable Finally, molecular biology a subcutaneous model were deeply analyze regulates progression. patients, positive within tumors larger than 2 cm diameter significantly higher smaller cm. Bioinformatics suggested NGEF, KIF4A, PABPC1 could be genes trigger are Subsequent co-culture neurons showed increased NGEF cells enhanced axonal neurons. Meanwhile, GSE30219 indicated exhibiting levels sizes, lymph node involvement, reduced overall survival rates. At level mechanisms, knockdown Ephrin-A3 ND7/23 or use ALW-II-41-27 resulted significant decrease neurite outgrowth when co-cultured LA795 cells. animal model, overexpression promoted fibers, these effects inhibited ALW-II-41-27. facilitates through Ephrin-A3/EphA2 pathway, suggesting promising target for disrupting interactions between tumors. Biomaterials focus on anticipated potential treatment option cancer.

Language: Английский

Exploring the mechanisms by which common inhalational anesthetics influence malignant tumor metastasis: A data mining study based on comparative toxicogenomic databases DOI Creative Commons
Yiyu Chen, Wenjun Ouyang,

Haitao Lv

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 289, P. 117660 - 117660

Published: Jan. 1, 2025

Surgery remains the primary treatment for solid malignant tumors, but controlling postoperative tumor recurrence and metastasis continues to be a major challenge. Understanding factors that influence after surgery, as well underlying biological mechanisms, is critical. Previous studies suggest anesthetic agents may increase risk of in patients with cancer, mechanisms these findings remain unclear. In this study, we utilized toxicogenomics comparative toxicogenomic databases analyze data explore potential by which three commonly used inhalational anesthetics-sevoflurane, isoflurane, halothane-might promote metastasis. The results identified 18 genes associated Functional enrichment analysis revealed anesthetics could cell migration activating signaling pathways such IL-17 necrosis factor pathways, thereby potentially inducing Moreover, constructing TF-mRNA network, predicted several transcription might play key roles anesthetic-induced total 87 regulatory relationships between mRNA. These offer new insights future vivo or vitro contribute better understanding relationship metastasis, providing valuable reference points clinical decision-making. study also provide determination subsequent targets. Hence, laboratory should prioritize investigating specific common study.

Language: Английский

Citations

0
Molecule interacting with CasL-2 enhances tumor progression and alters radiosensitivity in cervical cancer DOI Creative Commons
Yun Teng, Hongmei Zhao, Guoqing Xue

et al.

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 11, 2025

Cervical cancer is a common malignancy among women, and radiotherapy remains primary treatment modality across all disease stages. However, resistance to frequently results in failure, highlighting the need identify novel therapeutic targets improve clinical outcomes. The expression of molecule interacting with CasL-2 (MICAL2) was confirmed cervical tissues cell lines through western blotting (WB) immunohistochemistry (IHC). Siha Hela cells were used examine regulatory biological functions MICAL2 via knockdown overexpression experiments. Assays including MTT, colony formation, wound healing, transwell migration, sphere formation employed, along WB analysis. DNA damage irradiated or evaluated using comet assay, while γ-H2AX Rad51 protein levels detected by WB. In vivo experiments validated tumorigenic radioresistance MICAL2. Additionally, relationship between response analyzed 62 patients assessing tumor regression six months post-treatment. significantly elevated cells. Functional analyses demonstrated that promotes proliferation, invasion activating MAPK PI3K/AKT pathways, as both vitro Silencing increased damage, impeded repair, enhanced radiosensitivity. Among cancer, associated lower complete rate (25.6% vs. 60.9% those low expression), reduced progression-free survival, advanced stage (*p < 0.05). plays critical role progression cancer. These findings provide foundation for developing targeted therapies outcomes this population.

Language: Английский

Citations

0
NIR-II cascade-driven synergistic photodynamic and photothermal cancer therapies via 5-aminolevulinic acid-functionalized titanium carbide hexagonal nanorods DOI

Wu Yanlin,

Jingjing Zhang, Abdul Wahab

et al.

FlatChem, Journal Year: 2025, Volume and Issue: unknown, P. 100826 - 100826

Published: Jan. 1, 2025

Language: Английский

Citations

0
Epigallocatechin‐3‐Gallate Promotes Recanalization in Deep Vein Thrombosis by Modulating Endothelial Progenitor Cell Ferroptosis Through the Nrf2 Pathway DOI Open Access
Da Li, Youjun Mao, Xiaosong Zhang

et al.

Phytotherapy Research, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 7, 2025

Endothelial progenitor cells (EPCs) provide a promising therapeutic choice for deep venous thrombosis (DVT). Their number is increased by epigallocatechin-3-gallate (EGCG) in patients with diabetes. Although EGCG effective against doxorubicin-induced ferroptosis and cardiotoxicity, its efficacy DVT treatment has not been well studied. This study was aimed at assessing the effects of on EPC recanalization model. EPCs were treated EGCG, their proliferation migration, angiogenesis, apoptosis evaluated using cell counting kit-8 colony formation, Transwell, tube flow cytometry assays. Levels iron, markers, reactive oxygen species (ROS), mitochondrial membrane potential (ΔΨm) measured. Expression ferroptosis-related genes proteins analyzed qRT-PCR western blotting, respectively. Promoter activation dual-luciferase reporter system. Thrombus examined mouse model via hematoxylin eosin staining digital subtraction angiography. promoted proliferation, angiogenesis suppressed apoptosis. It attenuated reducing iron ROS accumulation, increasing ΔΨm, regulating expression (ALOX15, ACSL4, FTH1). enhanced Nrf2 targets, Slc7A11, HO-1, GPX4. inhibited thrombogenesis mice, an effect mediated through pathway upon transplantation. Transplantation EGCG-pretreated facilitates resolution blockade. EPC-based therapy may novel option DVT.

Language: Английский

Citations

0
Neuronal guanine nucleotide exchange factor promotes the axonal growth and cancer cell proliferation via Ephrin-A3/EphA2 axis in lung adenocarcinoma DOI Creative Commons
Jie Mi, Wentian Zhang,

Yijiu Ren

et al.

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 28, 2025

Neural infiltration has been found in various cancers and the infiltrating nerves influence tumor growth dissemination. In non-small cell lung cancer, pan-neuronal marker PGP9.5 was detected by immunohistochemical staining its high expression correlated with poor prognosis. However, existence of nerve fibers mechanism driving neural remains unclear. We first used to assess density patients adenocarcinoma different sizes. Following that, we performed differential analysis univariate Cox prognostic analysis, using public datasets experiments identify gene that triggers is associated cancer progression unfavorable Finally, molecular biology a subcutaneous model were deeply analyze regulates progression. patients, positive within tumors larger than 2 cm diameter significantly higher smaller cm. Bioinformatics suggested NGEF, KIF4A, PABPC1 could be genes trigger are Subsequent co-culture neurons showed increased NGEF cells enhanced axonal neurons. Meanwhile, GSE30219 indicated exhibiting levels sizes, lymph node involvement, reduced overall survival rates. At level mechanisms, knockdown Ephrin-A3 ND7/23 or use ALW-II-41-27 resulted significant decrease neurite outgrowth when co-cultured LA795 cells. animal model, overexpression promoted fibers, these effects inhibited ALW-II-41-27. facilitates through Ephrin-A3/EphA2 pathway, suggesting promising target for disrupting interactions between tumors. Biomaterials focus on anticipated potential treatment option cancer.

Language: Английский

Citations

0