New and emerging mechanisms of insecticide resistance

Current Opinion in Insect Science, Journal Year: 2024, Volume and Issue: 63, P. 101184 - 101184

Published: March 6, 2024

Language: Английский

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Dynamic Roles of Insect Carboxyl/Cholinesterases in Chemical Adaptation

Insects, Journal Year: 2023, Volume and Issue: 14(2), P. 194 - 194

Published: Feb. 16, 2023

Insects have evolved several intricate defense mechanisms to adapt their chemical environment. Due versatile capabilities in hydrolytic biotransformation, insect carboxyl/cholinesterases (CCEs) play vital roles the development of pesticide resistance, facilitating adaptation insects host plants, and manipulating behaviors through olfaction system. CCEs confer insecticide resistance qualitative or quantitative changes CCE-mediated enhanced metabolism target-site insensitivity, may contribute plant adaptation. represent first odorant-degrading enzymes (ODEs) discovered degrade pheromones odors remain most promising ODE candidates. Here, we summarize CCE classification, currently characterized protein structure characteristics, dynamic

Language: Английский

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Transfer and bioaccumulation of pesticides in terrestrial arthropods and food webs: State of knowledge and perspectives for research

Chemosphere, Journal Year: 2024, Volume and Issue: 357, P. 142036 - 142036

Published: April 12, 2024

Arthropods represent an entry point for pesticide transfers in terrestrial food webs, and accumulation upper chain organisms, such as predators can have cascading consequences on ecosystems. However, the mechanisms driving transfer bioaccumulation webs remain poorly understood. Here we review literature mediated by arthropods webs. The of pesticides their potential biomagnification are related to chemical properties toxicokinetic substances, resistance detoxification abilities contaminated well effects organisms' life history traits. We further identify four critical areas which knowledge gain would improve future predictions impacts First, efforts should be made regarding co-formulants mixtures that currently understudied. Second, progress sensitivity analytical methods allow detection low concentrations small individual arthropods. Quantifying preys, predators, or vertebrates at higher trophic level bring crucial insights into real-world Finally, quantifying influence structure complexity communities could address several important sources variability across species This narrative will inspire studies aiming quantify better capture ecological natural cultivated landscapes.

Language: Английский

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Glutathione S-transferase: A versatile and dynamic enzyme

Biochemical and Biophysical Research Communications, Journal Year: 2024, Volume and Issue: 734, P. 150774 - 150774

Published: Oct. 1, 2024

Language: Английский

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A nanoformulation that enhance the efficiency of camptothecin against lepidopteran pests by controlled release and ‘poison bait’ strategies

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 486, P. 150358 - 150358

Published: March 11, 2024

Language: Английский

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Differential detoxification enzyme profiles in C-corn strain and R-rice strain of Spodoptera frugiperda by comparative genomic analysis: insights into host adaptation

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: Jan. 6, 2025

The fall armyworm (FAW) Spodoptera frugiperda, a highly invasive, polyphagous pest, poses global agricultural threat. It has two strains, the C-corn and R-rice each with distinct host preferences. This study compares detoxification enzyme gene families across these strains related species to explore their adaptation diverse plant metabolites. A total of 1,995 detoxification-related genes, including cytochrome P450 monooxygenases (CYPs), carboxylesterases (COEs), glutathione S-transferases (GSTs), UDP-glucuronosyltransferases (UGTs), ATP-binding cassette transporters (ABCs), were identified genus Spodoptera, S. littoralis, litura, picta, exigua, both FAW strains. higher abundance phase I enzymes (CYPs COEs) GSTs was observed in species, while exhibited fewer notable differences copy numbers between C R Analyses at subfamily level revealed significant variation distribution expression, particularly within II enzymes. Expansions CYP6AE detected strain, contractions GST-ε, CYP9A, CYP4M, UGT33B, UGT33F occurred In contrast, no substantial III ABC Functional predictions protein interaction networks suggest broader expansion metabolism-related genes strain compared strain. These findings emphasize pivotal role adaptation, providing molecular insights into FAW's capacity for range expansion, which are crucial devising targeted sustainable pest management strategies. differential expression different frugiperda.

Language: Английский

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Expression profile of the entire detoxification gene inventory of the western honeybee, Apis mellifera across life stages

Pesticide Biochemistry and Physiology, Journal Year: 2023, Volume and Issue: 192, P. 105410 - 105410

Published: March 28, 2023

The western honeybee, Apis mellifera, is a managed pollinator of many crops and potentially exposed to wide range foreign compounds, including pesticides throughout its life cycle. Honeybees as well other insects recruit molecular defense mechanisms facilitate the detoxification xenobiotic compounds. inventory genes (DETOXome) comprised five protein superfamilies: cytochrome P450 monooxygenases (P450), carboxylesterases, glutathione S-transferases (GST), UDP-glycosyl transferases (UGT) ATP-binding cassette (ABC) transporters. Here we characterized gene expression profile entire honeybee DETOXome by analyzing 47 transcriptomes across cycle, different larval instars, pupae, adults. All stages were separated principal component analysis, K-means clustering revealed distinct temporal patterns expression. Indeed, >50% found in one cluster follows strikingly similar profiles, i.e., increased during development, followed sharp decline after pupation steep increase again This includes 29 dominated CYP3 CYP4 clan members, 15 ABC transporter mostly belonging ABCC subfamily 13 carboxylesterase almost all members involved dietary/detox hormone/semiochemical processing. RT-qPCR analysis selected from families high levels various tissues, especially Malpighian tubules, fatbody midgut, supporting view that these tissues are essential for metabolic clearance environmental toxins pollutants honeybees. Our study meant spark further research on basis this critical better understand evaluate negative impacts toxic substances. Additionally, set collected analyzed provides valuable resource future disciplines.

Language: Английский

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Filamentous Fungi as Bioremediation Agents of Industrial Effluents: A Systematic Review

Fermentation, Journal Year: 2024, Volume and Issue: 10(3), P. 143 - 143

Published: March 1, 2024

The industrial sector plays a significant role in global economic growth. However, it also produces polluting effluents that must be treated to prevent environmental damage and ensure the quality of life for future generations is not compromised. Various physical, chemical, biological methods have been employed treat effluents. Filamentous fungi, particular, garnered attention as effective bioremediation agents due their ability produce enzymes capable degrading recalcitrant compounds, adsorb different pollutant molecules. novelty work reported herein lies its comprehensive assessment research surrounding use white- brown-rot fungi removing phenolic compounds from This study employs systematic review coupled with scientometric analysis provide insights into evolution this technology over time. It scrutinizes geographical distribution, identifies gaps trends, highlights most studied fungal species applications. A 464 publications 1945 2023 assessed these White-rot were predominant (96.3%), notably Phanerochaete chrysosporium, Pleurotus ostreatus, Trametes versicolor, Lentinula edodes. cultures employing free cells (64.15%) stand out those using immobilized cells, just like isolated regarding systems microbial consortia. Geographically, Italy, Spain, Greece, India, Brazil emerged prominent countries related area during evaluated period.

Language: Английский

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Identification and Functional Characterization of an Omega-Class Glutathione S-Transferase Gene PcGSTO1 Associated with Cyetpyrafen Resistance in Panonychus citri (McGregor)

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(13), P. 7010 - 7020

Published: March 26, 2024

Cyetpyrafen is a recently developed acaricide. The citrus red mite, Panonychus citri (McGregor), has significant resistance to cyetpyrafen. However, the molecular mechanism underlying cyetpyrafen in P. remains unclear. Glutathione S-transferases (GSTs) play critical role arthropod pesticide resistance. This study showed that GSTs were potentially related of through synergistic experiments and enzyme activity analysis. An omega-family GST gene, PcGSTO1, was significantly up-regulated egg, nymph, adult stages cyetpyrafen-resistant strain. Additionally, silencing PcGSTO1 increased mortality recombinant demonstrated ability metabolize Our results indicated overexpression associated with citri, they also provided valuable information for managing citri.

Language: Английский

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Assessing the effects of a commercial fungicide and an herbicide, alone and in combination, on Apis mellifera: Insights from biomarkers and cognitive analysis

Chemosphere, Journal Year: 2024, Volume and Issue: 359, P. 142307 - 142307

Published: May 9, 2024

Agrochemicals play a vital role in protecting crops and enhancing agricultural production by reducing threats from pests, pathogens weeds. The toxicological status of honey bees can be influenced number factors, including pesticides. While extensive research has focused on the lethal sublethal effects insecticides individual colonies, it is important to recognise that fungicides herbicides also affect bee health. Unfortunately, field, are exposed mixtures compounds rather than single substances. This study aimed evaluate commercial fungicide herbicide, both individually combination, bees. Mortality assays, biomarkers learning memory tests were performed, results integrated assess Neurotoxicity (acetylcholinesterase carboxylesterase activities), detoxification metabolic processes (glutathione S-transferase alkaline phosphatase immune system function (lysozyme activity haemocyte count) genotoxicity (nuclear abnormalities assay) assessed. Sakura® was found activate enzymes activity. herbicide Elegant 2FD combination pesticides showed neurotoxic induced processes. Exposure herbicide/fungicide mixture impaired represents significant advance understanding commonly used agriculture contributes development effective strategies mitigate their adverse non-target insects.

Language: Английский

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