Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Oct. 18, 2024
Abstract
Clinical
studies
have
suggested
that
tirzepatide
may
also
possess
hepatoprotective
effects;
however,
the
molecular
mechanisms
underlying
this
association
remain
unclear.
In
our
study,
we
performed
biochemical
analyses
of
serum
and
histopathological
examinations
liver
tissue
in
mice.
To
preliminarily
explore
on
metabolic
dysfunction-associated
fatty
disease
(MAFLD),
liquid
chromatography-mass
spectrometry
(LC-MS)
was
employed
for
comprehensive
metabolomic,
lipidomic,
proteomic
MAFLD
mice
fed
a
high-fat
diet
(HFD).
The
results
demonstrated
significantly
reduced
levels
alanine
transaminase
(ALT)
aspartate
(AST),
as
well
hepatic
triglycerides
(TG)
total
cholesterol
(TC),
indicating
its
efficacy
treating
MAFLD.
Further
findings
revealed
acid
uptake
by
downregulating
Cd36
Fabp2/4,
enhance
mitochondrial-lysosomal
function
upregulating
Lamp1/2.
addition,
promoted
efflux
reabsorption
expression
Hnf4a,
Abcg5,
Abcg8.
These
suggest
exerts
therapeutic
effects
reducing
uptake,
promoting
excretion,
enhancing
function,
providing
theoretical
basis
understanding
tirzepatide.
Biomedicine & Pharmacotherapy,
Journal Year:
2024,
Volume and Issue:
174, P. 116432 - 116432
Published: March 22, 2024
Oxidative
stress
results
from
a
persistent
imbalance
in
oxidation
levels
that
promotes
oxidants,
playing
crucial
role
the
early
and
sustained
phases
of
DNA
damage
genomic
epigenetic
instability,
both
which
are
intricately
linked
to
development
tumors.
The
molecular
pathways
contributing
carcinogenesis
this
context,
particularly
those
related
double-strand
single-strand
breaks
DNA,
serve
as
indicators
due
cancer
cases,
well
factors
instability
through
ectopic
expressions.
has
been
considered
therapeutic
target
for
many
years,
an
increasing
number
studies
have
highlighted
promising
effectiveness
natural
products
treatment.
In
regard,
we
present
significant
research
on
targeting
oxidative
using
molecules
underscore
essential
cancer.
consequences
stress,
especially
also
offer
prospects.
use
epi-drugs
capable
modulating
reorganizing
network
is
beginning
emerge
remarkably.
review,
emphasize
close
connections
between
tumor
transformation,
while
highlighting
substances
antioxidants
anti-tumoral
context.
BMC Pharmacology and Toxicology,
Journal Year:
2025,
Volume and Issue:
26(1)
Published: Jan. 3, 2025
Abstract
Background
Naringenin,
a
flavonoid
compound
found
in
citrus
fruits,
possesses
valuable
anticancer
properties.
However,
its
potential
application
cancer
treatment
is
limited
by
poor
bioavailability
and
pharmacokinetics
at
tumor
sites.
To
address
this,
Naringenin
nanoparticles
(NARNPs)
were
prepared
using
the
emulsion
diffusion
technique
their
effects
investigated
HepG2
cells.
Methods
The
particle
size
of
NARNPs
was
determined
transmission
electron
microscopy
scanning
analysis.
NARNP
characterized
Fourier
transform
infrared
spectroscopy
X-ray
diffraction.
Study
cytotoxic
various
doses
naringenin,
DOX
on
WI38
cell
lines
after
24
h
48
MTT
assay.
Flow
cytometric
analysis
used
to
study
apoptotic
also
examined
expression
proteins
(p53)
autophagy-related
genes
ATG5,
LC3
with
NARNPs,
doxorubicin,
combinations
Results
analysis,
showing
mean
diameters
54.96
±
18.6
nm
31.79
6.8
nm,
respectively.
confirmed
successful
conjugation
between
naringenin
NARNPs.
an
amorphous
state
that
IC50
values
as
22.32
µg/ml
for
1.6
0.46
doxorubicin.
showed
induced
late
apoptosis
56.1%
cells
had
no
effect
97%
viable
incubation.
cycle
arrest
Go/G1
G2/M
phases
results
increased
LC3,
p53
treated
concentrations
enhanced
doxorubicin
but
decreased
Conclusions
demonstrated
effectively
inhibit
proliferation
induce
human
hepatocellular
carcinoma
Importantly,
normal
cells,
indicating
promising
therapy
hepatocarcinogenesis.
Combining
chemotherapy
drugs
could
present
novel
approach
treating
cancers.
Lipids in Health and Disease,
Journal Year:
2025,
Volume and Issue:
24(1)
Published: Jan. 10, 2025
Clinical
studies
have
suggested
that
tirzepatide
may
also
possess
hepatoprotective
effects;
however,
the
molecular
mechanisms
underlying
this
association
remain
unclear.
In
our
study,
we
performed
biochemical
analyses
of
serum
and
histopathological
examinations
liver
tissue
in
mice.
To
preliminarily
explore
on
metabolic
dysfunction-associated
fatty
disease
(MAFLD),
liquid
chromatography-mass
spectrometry
(LC-MS)
was
employed
for
comprehensive
metabolomic,
lipidomic,
proteomic
MAFLD
mice
fed
a
high-fat
diet
(HFD).
The
results
demonstrated
significantly
reduced
levels
alanine
transaminase
(ALT)
aspartate
(AST),
as
well
hepatic
triglycerides
(TG)
total
cholesterol
(TC),
indicating
its
efficacy
treating
MAFLD.
Further
findings
revealed
acid
uptake
by
downregulating
Cd36
Fabp2/4,
enhance
mitochondrial-lysosomal
function
upregulating
Lamp1/2.
addition,
promoted
efflux
reabsorption
expression
Hnf4a,
Abcg5,
Abcg8.
These
suggest
exerts
therapeutic
effects
reducing
uptake,
promoting
excretion,
enhancing
function,
providing
theoretical
basis
understanding
tirzepatide.
Discover Oncology,
Journal Year:
2025,
Volume and Issue:
16(1)
Published: Jan. 15, 2025
Rosmarinic
acid
(RosA)
is
a
natural
polyphenol
compound
that
has
been
shown
to
be
effective
in
the
treatment
of
inflammatory
disease
and
variety
malignant
tumors.
However,
its
specific
mechanism
for
lung
adenocarcinoma
(LUAD)
not
fully
elucidated.
Therefore,
this
study
aims
clarify
RosA
LUAD
by
integrating
bioinformatics,
network
pharmacology
vivo
experiments,
explore
potential
active
ingredients
traditional
Chinese
medicine
treating
LUAD.
Firstly,
was
used
screen
targets,
LUAD-related
differential
expressed
genes
(DEGs)
were
acquired
from
GEO
database.
The
intersection
regulated
(RDEGs)
obtained
through
Venn
diagram.
Secondly,
GO
KEGG
enrichment
analysis
RDEGs
performed,
protein–protein
interaction
networks
(PPIs)
constructed
identify
visualize
hub
RDEGs.
Then,
molecular
docking
between
further
evaluation
carried
out
using
bioinformatics
predictive
value
Finally,
verified
establishing
xenograft
model
NSCLC
nude
mouse.
Bioinformatics
other
showed
that,
compared
with
control
group,
expressions
MMP-1,
MMP-9,
IGFBP3
PLAU
tissues
significantly
up-regulated,
PPARG
FABP4
down-regulated,
these
had
In
experimental
results
could
inhibit
growth
transplanted
tumors
mice
bearing
cancer
cells,
reduce
positive
expression
Ki67
tumor
tissue,
hinder
proliferation
cells.
Upregulated
activating
PPAR
signaling
pathway
increases
level
ROS
promotes
apoptosis
addition,
can
also
MMP-9
IGFBP3,
migration
invasion
tissue
This
demonstrated
induce
regulating
proliferation,
thereby
exerting
anti-LUAD
effects.
provides
new
insight
into
therapeutic
avenue
Frontiers in Cell and Developmental Biology,
Journal Year:
2025,
Volume and Issue:
13
Published: Feb. 14, 2025
Introduction
Recent
research
indicated
a
strong
link
between
the
gut
microbiota
and
osteoarthritis.
However,
complex
interplay
microbiota,
serum
metabolites,
progression
of
osteoarthritis
in
affected
individuals
remains
largely
unexplored.
This
study
aimed
to
investigate
characteristics
metabolites
patients
with
Methods
Participants
either
healthy
knees
or
were
enrolled
categorized
into
control
(HC)
(OA)
groups.
Fecal
blood
samples
collected
for
16S
rRNA
gene
sequencing,
metabolomic
analysis
via
liquid
chromatography–mass
spectrometry
(LC-MS),
integrated
evaluation.
Results
The
results
showed
no
significant
variation
richness
diversity
two
abundance
Bacteroides
plebeius
Faecalibacterium
prausnitzii
was
reduced
OA
group,
both
which
are
known
their
potential
as
next-generation
probiotics
human
health.
Metabolomic
that
including
pyrogallol
3-hydroxybutyrate
(3HB),
significantly
lower
group.
These
positively
impact
other
diseases
demonstrated
good
diagnostic
performance
distinguishing
from
controls.
Correlation
revealed
positive
correlation
3HB.
Discussion
highlighted
specific
metabolite
profiles
patients,
suggesting
changes
bacteria
derived
closely
tied
progression.
underscores
modifiable
elements
therapeutic
targets
prevention.
