Berberine inhibits NLRP3 Inflammasome pathway in human triple-negative breast cancer MDA-MB-231 cell

BMC Complementary and Alternative Medicine, Journal Year: 2019, Volume and Issue: 19(1)

Published: Aug. 14, 2019

Breast cancer is still the most common malignant tumor that threatens female's life in world, especially triple-negative breast (TNBC), one of difficult subtypes. Lack targeted therapies brings about urgent demand for novel treatments. In this study we aim to investigate anti-tumor activity Berberine (BBR), a Chinese plant-derived alkaloid, against TNBC cell line MDA-MB-231 and elucidate its mechanism referring anti-inflammation. Cell inhibition rate was measured by Proliferation Assay, cytotoxic effects detected Lactate dehydrogenase (LDH) leakage assay, colony formation migration potential were evaluated assay wound healing release inflammatory cytokines EMD multifactor detection, alterations proteins genes related NLR family pyrin domain containing 3 (NLRP3) inflammasome pathway analyzed using western blotting real-time Polymerase Chain Reaction (PCR). BBR reduce viability cells increased LDH from dose-dependent manner, with cells. also caused marked reduction secretion proinflammatory cytokines, Interleukin-1α (IL-1α), Interleukin-1β (IL-1β), Interleukin-6 (IL-6), necrosis factor-α (TNF-α). Besides, down-regulated behavior observed expression P2X purinoceptor 7 (P2X7), NLRP3, pro-caspase-1, apoptosis-associated speck-like protein caspase-activation recruitment (ASC), caspase-1 p20, Interleukin-18 (IL-18), IL-1β Caspase-1 ASC mRNAs NLRP3 cascade. Our results confirmed can effectively affect both outgrowth spontaneous metastasis TNBC, identified new associated pathway, suggesting therapeutic relevance clinical use.

Language: Английский

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Impact of sodium glucose cotransporter 2 (SGLT2) inhibitors on atherosclerosis: from pharmacology to pre-clinical and clinical therapeutics

Theranostics, Journal Year: 2021, Volume and Issue: 11(9), P. 4502 - 4515

Published: Jan. 1, 2021

Sodium-glucose cotransporter 2 inhibitors (SGLT2i) are new oral drugs for the therapy of patients with type diabetes mellitus (T2DM).Research in past decade has shown that SGLT2i class, such as empagliflozin, canagliflozin, and dapagliflozin, have pleiotropic effects preventing cardiovascular diseases beyond their favorable impact on hyperglycemia.Of clinical relevance, recent landmark outcome trials demonstrated reduce major adverse events, hospitalization heart failure, death T2DM with/without (including atherosclerotic various types failure).The pharmacological action is through inhibiting glucose re-absorption kidney thus promoting excretion.Studies experimental models atherosclerosis ameliorate progression by mechanisms including inhibition vascular inflammation, reduction oxidative stress, reversing endothelial dysfunction, reducing foam cell formation platelet activation.Here, we summarize anti-atherosclerotic actions SGLT2i, an aim to emphasize utility this class agents insidious complications accompanying diabetes.

Language: Английский

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Antioxidant Therapy and Antioxidant-Related Bionanomaterials in Diabetic Wound Healing

Frontiers in Bioengineering and Biotechnology, Journal Year: 2021, Volume and Issue: 9

Published: June 24, 2021

Ulcers are a lower-extremity complication of diabetes with high recurrence rates. Oxidative stress has been identified as key factor in impaired diabetic wound healing. Hyperglycemia induces an accumulation intracellular reactive oxygen species (ROS) and advanced glycation end products, activation metabolic pathways, such the polyol pathway, PKC signaling leading to suppression antioxidant enzymes compounds. Excessive uncontrolled oxidative impairs function cells involved healing process, resulting chronic non-healing wounds. Given central role pathology ulcers, we performed comprehensive review on mechanism healing, focusing progress therapeutics. We summarize therapies proposed past 5 years for use including Nrf2- NFκB-pathway-related therapy, vitamins, enzymes, hormones, medicinal plants, biological materials.

Language: Английский

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Berberine: A Review of its Pharmacokinetics Properties and Therapeutic Potentials in Diverse Vascular Diseases

Frontiers in Pharmacology, Journal Year: 2021, Volume and Issue: 12

Published: Nov. 3, 2021

Traditional Chinese medicine plays a significant role in the treatment of various diseases and has attracted increasing attention for clinical applications. Vascular affecting vasculature heart, cerebrovascular disease, atherosclerosis, diabetic complications have compromised quality life affected individuals increase burden on health care services. Berberine, naturally occurring isoquinoline alkaloid form Rhizoma coptidis , is widely used China as folk its antibacterial anti-inflammatory properties. Promisingly, an number studies identified several cellular molecular targets berberine, indicating potential alternative therapeutic strategy vascular diseases, well providing novel evidence that supports berberine to combat diseases. The purpose this review comprehensively systematically describe agent including pharmacological effects, mechanisms, pharmacokinetics. According data published so far, shows remarkable anti-inflammatory, antioxidant, antiapoptotic, antiautophagic activity via regulation multiple signaling pathways, AMP-activated protein kinase (AMPK), nuclear factor κB (NF-κB), mitogen-activated silent information regulator 1 (SIRT-1), hypoxia-inducible 1α (HIF-1α), endothelial growth phosphoinositide 3-kinase (PI3K), B (Akt), janus 2 (JAK-2), Ca 2+ channels, endoplasmic reticulum stress. Moreover, we discuss existing limitations give corresponding measures. In addition, propose some research perspectives challenges, provide solid base from which further can excavate effective drugs monomers.

Language: Английский

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Berberine improves intestinal barrier function and reduces inflammation, immunosuppression, and oxidative stress by regulating the NF-κB/MAPK signaling pathway in deoxynivalenol-challenged piglets

Environmental Pollution, Journal Year: 2021, Volume and Issue: 289, P. 117865 - 117865

Published: July 31, 2021

Language: Английский

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Targeting the crosstalk between canonical Wnt/β-catenin and inflammatory signaling cascades: A novel strategy for cancer prevention and therapy

Pharmacology & Therapeutics, Journal Year: 2021, Volume and Issue: 227, P. 107876 - 107876

Published: April 27, 2021

Language: Английский

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Traditional Chinese Medicine and Colorectal Cancer: Implications for Drug Discovery

Frontiers in Pharmacology, Journal Year: 2021, Volume and Issue: 12

Published: July 1, 2021

As an important part of complementary and alternative medicine, traditional Chinese medicine (TCM) has been applied to treat a host diseases for centuries. Over the years, with incidence rate human colorectal cancer (CRC) increasing continuously advantage TCM gradually becoming more prominent, importance in both domestic international fields is also growing each passing day. However, unknowability active ingredients, effective substances, underlying mechanisms against this malignant tumor greatly restricts translation degree clinical products pace precision medicine. In review, based on characteristics oral administration most we herein provide beneficial information utilization prevention treatment CRC retrospect current preclinical studies related as well put forward research mode discovery ingredients substances TCM, novel insights into development innovative agents from conventional assist realization

Language: Английский

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Gut microbiota specifically mediates the anti-hypercholesterolemic effect of berberine (BBR) and facilitates to predict BBR’s cholesterol-decreasing efficacy in patients

Journal of Advanced Research, Journal Year: 2021, Volume and Issue: 37, P. 197 - 208

Published: July 30, 2021

Gut microbiota has been implicated in the pharmacological activities of many natural products. As an effective hypolipidemic agent, berberine (BBR)'s clinical application is greatly impeded by obvious inter-individual response variation. To date, little evidence exists on causality between gut microbes and its therapeutic effects, linkage bacteria alterations to variation.This study aims confirm causal role BBR's anti-hyperlipidemic effect identify key that can predict effectiveness.The correlation variation was studied hyperlipidemic patients. The effects subsequently assessed altered administration routes, co-treatment with antibiotics, fecal transplantation, metagenomic analysis.Three-month showed BBR effectively decrease serum lipids but displayed cholesterol-lowering not triglyceride-decreasing closely related modulation microbiota. Interestingly, baseline levels Alistipes Blautia could accurately anti-hypercholesterolemic efficiency following treatment. Causality experiments mice further confirmed microbiome both necessary sufficient mediate lipid-lowering BBR. absence substantially abolished cholesterol-decreasing efficacy.The for hyperlipidemia-ameliorating effect. composition be predictor pharmacotherapeutic efficacy, providing a novel way achieve personalized therapy.

Language: Английский

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Berberine: A Promising Treatment for Neurodegenerative Diseases

Frontiers in Pharmacology, Journal Year: 2022, Volume and Issue: 13

Published: May 20, 2022

Berberine, as a natural alkaloid compound, is characterized by diversity of pharmacological effects. In recent years, many researches focused on the role berberine in central nervous system diseases. Among them, effect neurodegenerative diseases has received widespread attention, for example Alzheimer’s disease, Parkinson’s Huntington’s and so on. Recent evidence suggests that inhibits production neuroinflammation, oxidative, endoplasmic reticulum stress. These effects can further reduce neuron damage apoptosis. Although current research made some progress, its specific mechanism still needs to be explored. This review provides an overview related mechanisms, also new ideas future berberine.

Language: Английский

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Expatiating the Pharmacological and Nanotechnological Aspects of the Alkaloidal Drug Berberine: Current and Future Trends

Molecules, Journal Year: 2022, Volume and Issue: 27(12), P. 3705 - 3705

Published: June 9, 2022

Traditionally, herbal compounds have been the focus of scientific interest for last several centuries, and continuous research into their medicinal potential is underway. Berberine (BBR) an isoquinoline alkaloid extracted from plants that possess a broad array properties, including anti-diarrheal, anti-fibrotic, antidiabetic, anti-inflammatory, anti-obesity, antihyperlipidemic, antihypertensive, antiarrhythmic, antidepressant, anxiolytic effects, frequently utilized as traditional Chinese medicine. BBR promotes metabolisms glucose lipids by activating adenosine monophosphate-activated protein kinase, stimulating glycolysis inhibiting functions mitochondria; all these ameliorate type 2 diabetes mellitus. has also shown to benefits in congestive heart failure, hypercholesterolemia, atherosclerosis, non-alcoholic fatty liver disease, Alzheimer’s polycystic ovary syndrome. investigated interesting pharmacophore with contribute significantly development novel therapeutic medicines variety disorders. Despite its enormous promise, clinical application this was severely limited because unpleasant pharmacokinetic characteristics. Poor bioavailability, absorption, poor water solubility are some obstacles restricted use. Nanotechnology suggested possible solution problems. The present review aims at recent updates on important activities different types nanocarriers used delivery diseases.

Language: Английский

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