Dissolving microneedle patch loaded with adipokines-enriched adipose extract relieves atopic dermatitis in mouse via modulating immune disorders, microbiota imbalance, and skin barrier defects DOI Creative Commons
Jingyan Guan, Kaiqi Chen,

Feng Lu

et al.

Journal of Tissue Engineering, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 1, 2025

Atopic dermatitis (AD) is a chronic relapsing dermatosis that demands new therapies. This research group previously developed physically extracted adipose-derived extracellular matrix named adipose collagen fragments (ACF), which was determined containing massive matrix-bound adipokines and medicable on AD through intradermal injection. However, problems concerning the control of drug release inevitable pain caused by injection hinder application ACF in clinics. Microneedle (MN) rapid developing technique for precise painless transdermal delivery. Therefore, dissolving methacrylated gelatin/hyaluronic acid MN patch loaded with this study. The morphological characteristics, mechanical properties, penetration ability, as well biocompatibility degradation efficiency ACF-MN were evaluated, its efficacy ovalbumin-induced mice also investigated. exhibited excellent biocompatibility, efficiency, satisfying murine similar fresh-made ACF. Furthermore, RNA-Seq combining bioinformatics performed mechanism exploration. treatment showed comprehensive via restoring inflammatory dysregulation, microbiota imbalance, skin barrier defects. study offered novel MN-based ACF-bound delivery system may serve promising strategy relieving AD.

Language: Английский

Reactive Oxygen Species-Responsive Gel-Based Microneedle Patches for Prolonged and Intelligent Psoriasis Management DOI

Duohang Bi,

Fei Qu, Wanyue Xiao

et al.

ACS Nano, Journal Year: 2023, Volume and Issue: 17(5), P. 4346 - 4357

Published: Feb. 27, 2023

Psoriasis is an inflammatory skin disease. Microneedle (MN) patches can improve psoriasis treatment outcomes by increasing local drug content in the skin. As frequently relapses, developing intelligent MN-based delivery systems with prolonged therapeutic levels and improved efficiency of great significance. Here, we designed detachable H2O2-responsive gel-based MN containing methotrexate (MTX) epigallocatechin gallate (EGCG) using EGCG as both cross-linkers for needle-composited materials anti-inflammatory drugs. The MNs had dual-mode release kinetics, which quickly released MTX diffusively sustainably way. Compared dissolving MNs, extended retention EGCG, leading to reactive oxygen species (ROS) scavenging effects. ROS-responsive that transdermally delivered antiproliferative drugs psoriasis-like prophylactic animal models.

Language: Английский

Citations

80
Design principles of microneedles for drug delivery and sampling applications DOI
Zhicheng Le, Jinming Yu, Ying Jie Quek

et al.

Materials Today, Journal Year: 2022, Volume and Issue: 63, P. 137 - 169

Published: Nov. 17, 2022

Language: Английский

Citations

76
The next-generation DNA vaccine platforms and delivery systems: advances, challenges and prospects DOI Creative Commons
Bowen Lu,

Jing Ming Lim,

Boyue Yu

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 1, 2024

Vaccines have proven effective in the treatment and prevention of numerous diseases. However, traditional attenuated inactivated vaccines suffer from certain drawbacks such as complex preparation, limited efficacy, potential risks others. These limitations restrict their widespread use, especially face an increasingly diverse range With ongoing advancements genetic engineering vaccines, DNA emerged a highly promising approach both diseases acquired While several demonstrated substantial success animal models diseases, challenges need to be addressed before application human subjects. The primary obstacle lies absence optimal delivery system, which significantly hampers immunogenicity vaccines. We conduct comprehensive analysis current status by focusing on viral non-viral systems, they play crucial roles exploration novel provide evaluation strengths weaknesses based our critical assessment. Additionally, review summarizes most recent breakthroughs pre-clinical clinical studies, highlighting for further trials this rapidly evolving field.

Language: Английский

Citations

47
Exosomes: The emerging mechanisms and potential clinical applications in dermatology DOI Creative Commons
Honghao Yu,

Heting Feng,

Hong Zeng

et al.

International Journal of Biological Sciences, Journal Year: 2024, Volume and Issue: 20(5), P. 1778 - 1795

Published: Jan. 1, 2024

Skin tissue, composed of epidermis, dermis, and subcutaneous is the largest organ human body.It serves as a protective barrier against pathogens physical trauma plays crucial role in maintaining homeostasis.Skin diseases, such psoriasis, dermatitis, vitiligo, are prevalent can seriously impact quality patient life.Exosomes lipid bilayer vesicles derived from multiple cells with conserved biomarkers important mediators intercellular communication.Exosomes skin cells, blood, stem main types exosomes that involved modulating microenvironment.The dysregulation exosome occurrence transmission, well alterations their cargoes, complex pathogenesis inflammatory autoimmune diseases.Therefore, promising diagnostic therapeutic targets for diseases.Importantly, exogenous exosomes, or play improving environment repairing damaged tissues by carrying various specific active substances involving variety pathways.In domain clinical practice, have garnered attention prospective agents including psoriasis vitiligo.Furthermore, investigations substantiated regenerative efficacy cell-derived repair.In this review, we mainly summarize latest studies about mechanisms applications dermatology, atopic systemic lupus erythematosus, sclerosis, diabetic wound healing, hypertrophic scar keloid, aging.This will provide novel perspective diagnosis treatment dermatosis.

Language: Английский

Citations

21
Microneedles as Transdermal Drug Delivery System for Enhancing Skin Disease Treatment DOI Creative Commons

Chaoxiong Wu,

Qingyu Yu,

Chenlu Huang

et al.

Acta Pharmaceutica Sinica B, Journal Year: 2024, Volume and Issue: 14(12), P. 5161 - 5180

Published: Aug. 23, 2024

Microneedles (MNs) serve as a revolutionary paradigm in transdermal drug delivery, heralding viable resolution to the formidable barriers presented by cutaneous interface. This review examines MNs an advanced approach enhancing dermatological pathology management. It explores complex dermis structure and highlights limitations of traditional methods, emphasizing MNs' advantage bypassing stratum corneum deliver drugs directly subdermal matrix. The discourse outlines diverse typologies MNs, including solid, coated, hollow, hydrogel, dissolvable versions. Each type is characterized its unique applications benefits. treatise details deployment alleviation cancers, administration inflammatory dermatoses such psoriasis atopic dermatitis, their utility wound Additionally, paper contemplates prospects within realm aesthetic dermatology burgeoning market traction cosmetic MN formulations. summarizes scientific commercial challenges clinical adoption therapeutics, dosage calibration, pharmacodynamics, biocompatibility, patient compliance, sterilization, mass production, regulatory oversight. emphasizes need for ongoing research, innovation, harmonization overcome these obstacles fully realize potential treating skin diseases improving welfare. presents comprehensive overview recent accomplishments, remaining challenges, future microneedle-facilitated therapy afflictions.

Language: Английский

Citations

20
Transdermal Drug Delivery System: Current Status and Clinical Application of Microneedles DOI
Yanan Xue, Cheng Chen,

Xiangyu Mi

et al.

ACS Materials Letters, Journal Year: 2024, Volume and Issue: 6(3), P. 801 - 821

Published: Feb. 2, 2024

As a new type of transdermal drug delivery system (TDDS), microneedles have garnered significant attention for their noninvasiveness, portability, controllable administration, and wide-ranging loading capacity. While initially designed administration via the stratum corneum minimizing adverse reactions associated with systemic enhancing patient compliance, recently found applications in multitude medical contexts. Hence, potential transcends mere delivery. Recent reviews on extensively covered various aspects, including needle types, materials, manufacturing methods, as well treating skin diseases. This review aims to provide comprehensive overview diverse designs from different perspectives, which encompass traditional integrated more intelligent structurally optimized ones nowadays. Additionally, this offers analysis material properties utilized applications, nondegradable materials degradable materials. Furthermore, presents these scenarios such regeneration repair, disease treatment, monitoring, prevention. Moreover, discussion article highlights future clinical necessary efforts required achieve widespread adoption. serves valuable reference bridging gap between laboratory setting practice terms using scenarios.

Language: Английский

Citations

19
Hydrogel-Coated SERS Microneedles for Drug Monitoring in Dermal Interstitial Fluid DOI
Li Yan, Yunqing Wang,

Rongchao Mei

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(5), P. 2567 - 2574

Published: May 2, 2024

In vivo drug monitoring is crucial for evaluating the effectiveness and safety of treatment. Blood sampling analysis current gold standard but needs professional skills cannot meet requirements point-of-care testing. Dermal interstitial fluid (ISF) showed great potential to replace blood in monitoring; however, detection was challenging, distribution behavior ISF still unclear until now. this study, we proposed surface-enhanced Raman spectroscopy (SERS) microneedles (MNs) painless real-time drugs after intravenous injection. Using methylene blue (MB) mitoxantrone (MTO) as model drugs, innovative core–satellite structured Au@Ag SERS substrate, hydrogel coating over MNs, rendered sensitive quantitative mice within 10 min. Based on technique, pharmacokinetics two investigated compared with those blood, where were analyzed via liquid chromatography–mass spectrometry. It found that MB concentration comparable, whereas MTO 2–3 orders magnitude lower than blood. This work an efficient tool monitoring. More importantly, it experimentally proved penetration ratio drug-dependent, providing insightful information into a alternative detection.

Language: Английский

Citations

19
Photo-responsive polydopamine nanoenzyme microneedles with oxidative stress regulation ability for atopic dermatitis treatment DOI
Yuanyuan Zhang, Xiao‐Xuan Zhang,

Xiangyi Wu

et al.

Nano Today, Journal Year: 2024, Volume and Issue: 56, P. 102241 - 102241

Published: March 28, 2024

Language: Английский

Citations

16
Construction of multifunctional hydrogel with metal-polyphenol capsules for infected full-thickness skin wound healing DOI Creative Commons
Nanbo Liu, Shuoji Zhu,

Yuzhi Deng

et al.

Bioactive Materials, Journal Year: 2022, Volume and Issue: 24, P. 69 - 80

Published: Dec. 13, 2022

Damaged skin cannot prevent harmful bacteria from invading tissues, causing infected wounds or even severe tissue damage. In this study, we developed a controlled-release antibacterial composite hydrogel system that can promote wound angiogenesis and inhibit inflammation by sustained releasing Cu-Epigallocatechin-3-gallate (Cu-EGCG) nano-capsules. The prepared SilMA/HAMA/Cu-EGCG showed an obvious inhibitory effect on Escherichia coli (E. coli) Staphylococcus aureus (S. aureus). It could also the proliferation migration of L929 fibroblasts. vivo full-thickness healing experiments confirmed regulating effect. Accelerate collagen deposition speed were observed in treated group. findings study show great potential application chronic healing.

Language: Английский

Citations

65
Safety Challenges and Application Strategies for the Use of Dendrimers in Medicine DOI Creative Commons
Xiang Li, Abid Naeem, Shanghua Xiao

et al.

Pharmaceutics, Journal Year: 2022, Volume and Issue: 14(6), P. 1292 - 1292

Published: June 17, 2022

Dendrimers are used for a variety of applications in medicine but, due to their host–guest and entrapment characteristics, particularly the delivery genes drugs. However, dendrimers intrinsically toxic, thus creating major limitation use biological systems. To reduce such toxicity, biocompatible have been designed synthesized, surface engineering has create advantageous changes at periphery dendrimers. Although reviewed previously literature, there yet be systematic comprehensive review harmful effects In this review, we describe routes dendrimer exposure distribution vivo. Then, discuss toxicity organ, cellular, sub-cellular levels. also how technology can by changing size functionalization, combined with other materials generate composite formulation, diagnosis disease. Finally, future challenges, developments, research directions developing safe medical purposes.

Language: Английский

Citations

52