Potential therapeutic strategy for cancer: Multi-dimensional cross-talk between circRNAs and parental genes

Cancer Letters, Journal Year: 2024, Volume and Issue: 588, P. 216794 - 216794

Published: March 6, 2024

Language: Английский

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Targeting circFOXO3 to Modulate Integrin β6 Expression in Periodontitis: A Potential Therapeutic Approach

Journal Of Clinical Periodontology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 12, 2025

ABSTRACT Aims Circular RNA forkhead box O3 (circFOXO3) is crucial in regulating inflammation lung and heart injuries. However, its role periodontitis remains unclear. We sought to elucidate the effects of circFOXO3 on progression related molecular mechanisms. Methods Reverse‐transcription quantitative polymerase chain reaction fluorescence situ hybridization were used quantify localize expression. The mechanism by which promotes was investigated using epithelial cells, human gingival epithelium a rat model ligature‐induced periodontitis. Results expression abnormally high tissues patients with Elevated levels down‐regulated microRNA (miR)‐141‐3p, leading increased FOXO3 interacted JunB form transcriptional‐repression complex that inhibited integrin β6 (ITGβ6)‐mediated activation transforming growth factor β (TGF‐β) cells. Through miR‐141‐3p/FOXO3/JunB axis, suppressed TGF‐β signalling, thereby exacerbating periodontal inflammation. Finally, inhibition hindered disease restored activity vivo via FOXO3/JunB/ITGβ6 pathway. Conclusion Our study identified novel contributes through transcriptional regulatory network involving miR‐141‐3p, FOXO3, ITGβ6. These findings highlight potential therapeutic targets for development effective treatments this debilitating disease.

Language: Английский

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Airway basal stem cell therapy for lung diseases: an emerging regenerative medicine strategy

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 29, 2025

Chronic pulmonary diseases pose a prominent health threat globally owing to their intricate pathogenesis and lack of effective reversal therapies. Nowadays, lung transplantation stands out as feasible treatment option for patients with end-stage disease. Unfortunately, the use this is limited by donor organ shortage severe immunological rejection reactions. Recently, airway basal stem cells (BSCs) have emerged novel therapeutic strategy in regenerative medicine because substantial potential repairing structure function. Airway BSCs, which are strongly capable self-renewal multi-lineage differentiation, can effectively attenuate epithelial injury caused environmental factors or genetic disorders, such cystic fibrosis. This review comprehensively explores efficacy action mechanisms BSCs across various disease models describes strategies inducing pluripotent differentiate into lineages on basis original research findings. Additionally, also discusses technical biological challenges translating these findings clinical applications offers prospective views future directions, therefore broadening landscape medicine.

Language: Английский

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Incorporation of forsterite nanoparticles in a 3D printed polylactic acid/polyvinylpyrrolidone scaffold for bone tissue regeneration applications

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 141046 - 141046

Published: Feb. 1, 2025

Language: Английский

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Oxidative stress-induced circSOD2 inhibits osteogenesis through sponging miR-29b in metabolic-associated fatty liver disease

World Journal of Gastroenterology, Journal Year: 2025, Volume and Issue: 31(9)

Published: Feb. 18, 2025

Metabolic-associated fatty liver disease (MAFLD) is characterized by lipid accumulation in hepatocytes and closely associated with oxidative stress. Increasing clinical evidence indicates that MAFLD linked to bone metabolic disorders, including osteoporosis. Recent studies indicate the expression profiles of circular RNAs (circRNAs) are altered MAFLD. However, effects these changes on metabolism remain poorly understood. To investigate mechanism differently expressed circRNAs secreted osteogenic differentiation RNA sequencing was performed identify highly liver, validated quantitative real-time reverse transcription polymerase chain reaction, localized using fluorescence situ hybridization (FISH). A mouse model induced a high-fat diet used simulate CircSOD2 significantly upregulated tissues primary from subjects stress attenuated antioxidants model. In addition, circSOD2 delivered marrow mesenchymal stem cells (BMSCs). Furthermore, inhibited BMSCs vivo formation sponging miR-29b. Moreover, miR-29b inhibition reversed stimulatory effect silencing formation. Mechanistically, interaction between confirmed through luciferase reporter assay co-localization cytoplasm evidenced FISH indicated acted as sponge for This study provides novel underlying liver-bone crosstalk, demonstrating upregulation hepatocytes, stress, inhibits These findings offer better understanding relationship

Language: Английский

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Circ_0070934 Regulates the Proliferation, Metastasis, and Epithelial–Mesenchymal Transition of Colorectal Cancer Cells by Targeting miR‐203a‐3p/HOXA13 Axis

Journal of Biochemical and Molecular Toxicology, Journal Year: 2025, Volume and Issue: 39(2)

Published: Feb. 1, 2025

ABSTRACT The present work explored the functions of circ_0070934 in regulating malignant phenotype colorectal cancer (CRC) cells and its underlying mechanisms. Gene expression data set was acquired based on Expression Omnibus (GEO) database for examining levels within CRC tissues through quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR). Kaplan–Meier curve log‐rank test were adopted assessing patient prognosis level. Functional assays including Cell Counting Kit (CCK)‐8, EdU incorporation, Transwell invasion, scratch conducted to determine cell malignancy. Molecular interactions predicted using circInteractome StarBase databases, validated luciferase reporter assay. Circ_0070934 upregulated tissues, which related a dismal prognostic outcome patients. Knocking down inhibited proliferation, epithelial–mesenchymal transition (EMT), migration. Further, we identified miR‐203a‐3p as target miRNA circ_0070934, negatively regulated Homeobox A13 (HOXA13) expression. miR‐203a‐3p/HOXA13 axis mediates function modulating These revealed that important maintaining cells, knockdown undermined Targeting circ_0070934/miR‐203a‐3p/HOXA13 is promising intervention approach managing CRC.

Language: Английский

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Long Non-Coding RNA EPB41L4A-AS1 Serves as a Diagnostic Marker for Chronic Periodontitis and Regulates Periodontal Ligament Injury and Osteogenic Differentiation by Targeting miR-214-3p/YAP1

Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 2483 - 2497

Published: Feb. 1, 2025

Several long non-coding RNAs (lncRNAs) are dysregulated in chronic periodontitis (CP). The study aimed to elucidate the molecular mechanisms and clinical significance of lncRNA EPB41L4A antisense RNA 1 (EPB41L4A-AS1) CP. This enrolled 101 patients with CP 90 subjects healthy periodontal tissues. Patients were categorized according severity. expression EPB41L4A-AS1 osteogenic markers lipopolysaccharide (LPS)-induced human ligament cells (hPDLCs) was assessed using real-time quantitative reverse transcription PCR (RT-qPCR). diagnostic evaluated receiver operating characteristic (ROC) analysis. levels inflammatory factors measured an enzyme-linked immunosorbent assay. Cell proliferation apoptosis analyzed cell counting kit -8 flow cytometry, respectively. interaction between microRNAs verified dual luciferase reporter assays, immunoprecipitation, pull-down assays. downregulated gingival sulcus fluid LPS-induced hPDLCs. Additionally, could distinguish from control sensitivity (88.12%) specificity (81.11%). severe downregulation directly correlated indicators inversely indicators. overexpression promoted differentiation hPDLCs mitigated inflammation. Mechanistically, targets downregulates miR-214-3p expression, resulting upregulation Yes1-associated transcriptional regulator (YAP1) levels. partially suppressed effects on hPDLC injury differentiation. enhanced through miR-214-3p/YAP1 axis. Thus, is a novel marker therapeutic target for

Language: Английский

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Genetically predicted plasma metabolites mediate the causal relationship between gut microbiota and osteosarcoma

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 1, 2025

Previous research has demonstrated a close connection between the development of osteosarcoma (OS) and variations in abundance specific gut microbiota (GM). Generally speaking, GM play role human health mostly through metabolites. However, causal relationship GM, plasma metabolites, OS remains unclear. Hence, our study, we aim to clarify this OS, by employing Mendelian randomization (MR) approach. In pooled data were derived from public genome-wide association study (GWAS) (GCST90032172 GCST90032644), metabolites (GCST90199621 GCST90204603) (finngen_R10_C3_OSTEOSARCOMA_EXALLC). The two-sample two-step MR methods used for current analysis: (1) genetic causality on OS; (2) mediation effects For evaluating previously described relationship, inverse variance weighted (IVW) method was primarily used, with complementary approaches including median, MR-Egger, mode, simple mode. Moreover, MR-Egger intercept test mendelian pleiotropy residual sum outlier (MR-PRESSO) employed assess horizontal multiplicity. reliability is verified "leave-one-out" sensitivity analysis Cochran's Q heterogeneity. STROBE-MR checklist reporting studies study. First, according IVW results, 13 types specifically, identified have potential OS. After FDR correction, Phocea defined as strain clear (FDR-adjusted p < 0.05). Second, total 48 30 currently known 7 not yet studied, 11 metabolite ratios. Finally, further explored whether mediate And result, two Eugenol sulfate levels (mediated proportion: 7.74% (14.2%, 1.3%)) N-acetylphenylalanine 3.52% (6.18%, 0.867%)), that may link identified. All above results subjected analysis. revealed Importantly, also mediating modulating Of course, needs be conducted verify findings.

Language: Английский

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Electrospinning-based bone tissue scaffold construction: Progress and trends

Materials & Design, Journal Year: 2025, Volume and Issue: unknown, P. 113792 - 113792

Published: March 1, 2025

Language: Английский

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CircAars‐Engineered ADSCs Facilitate Maxillofacial Bone Defects Repair Via Synergistic Capability of Osteogenic Differentiation, Macrophage Polarization and Angiogenesis

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Abstract Adipose‐derived stem cells (ADSCs) hold significant promise in bone tissue engineering due to their self‐renewal capacity and easy accessibility. However, limited osteogenic potential remains a critical challenge for clinical application repair. Emerging evidence suggests that circular RNAs (circRNAs) play key role regulating cell fate osteogenesis. Despite this, the specific mechanisms by which circRNAs influence ADSCs context of are largely unexplored. This study introduces novel strategy utilizing circAars, circRNA, modify ADSCs, then incorporated into gelatin methacryloyl (GelMA) hydrogels repair critical‐sized maxillofacial defects. The findings reveal circAars predominantly localizes cytoplasm where it acts as competitive sponge miR‐128‐3p, enhancing differentiation migration capabilities ADSCs. Furthermore, circAars‐engineered facilitate macrophage polarization from M1 M2 phenotype enhance endothelial (EC) angiogenic through paracrine mechanism. Additionally, GelMA scaffolds loaded with accelerate defects synergistically promoting osteogenesis, polarization, angiogenesis. approach offers promising therapeutic treatment

Language: Английский

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