Pro-Inflammatory Characteristics of Extracellular Vesicles in the Vitreous of Type 2 Diabetic Patients DOI Creative Commons
Shengshuai Shan,

Abdulaziz H. Alanazi,

Yohan Han

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(9), P. 2053 - 2053

Published: Sept. 10, 2024

Diabetic retinopathy (DR) is a leading cause of blindness, yet its molecular mechanisms are unclear. Extracellular vesicles (EVs) contribute to dysfunction in DR, but the characteristics and functions vitreous EVs This study investigated inflammatory properties type 2 diabetic (db) EVs. isolated from db non-db donors were used for nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), immunogold staining, Western blotting, proteomic by mass spectrometry. Intracellular uptake differentiated macrophages was evaluated using ExoGlow membrane labeling, impact on macrophage (THP-1) activation assessed cytokine levels RT-qPCR. NTA TEM showed non-aggregated with heterogeneous size range below 200 nm. blot detected EV markers (Alix, Annexin V, HSP70, Flotillin 1) an upregulation Cldn5 While incorporated into macrophages, treatment THP-1 cells significantly increased mRNA TNFα IL-1β compared Proteomic gene enrichment indicated pro-inflammatory Our results suggest potential involvement EC-derived Cldn5+ triggering inflammation, offering novel mechanism involved presenting possible therapeutic avenue DR.

Language: Английский

Contribution of extracellular vesicles for the pathogenesis of retinal diseases: shedding light on blood-retinal barrier dysfunction DOI Creative Commons
Beatriz Martins, Maria E. Pires, António Francisco Ambrósio

et al.

Journal of Biomedical Science, Journal Year: 2024, Volume and Issue: 31(1)

Published: May 10, 2024

Abstract Retinal degenerative diseases, including diabetic retinopathy (DR) and age-related macular degeneration (AMD), loom as threats to vision, causing detrimental effects on the structure function of retina. Central understanding these is compromised state blood-retinal barrier (BRB), an effective that regulates influx immune inflammatory components. Whether BRB breakdown initiates retinal distress, or a consequence disease progression, remains enigmatic. Nevertheless, it indication dysfunction potential vision loss. The intricate intercellular dialogues among cell populations remain unintelligible in complex milieu, under conditions inflammation oxidative stress. retina, specialized neural tissue, sustains ceaseless demand for oxygen nutrients from two vascular networks. orchestrates exchange molecules fluids within this region, comprising inner (iBRB) outer (oBRB). Extracellular vesicles (EVs) are small membranous structures, act messengers facilitating communication milieu. EVs, both peripheral cells, increase complexity DR AMD. Laden with bioactive cargoes, EVs can modulate microenvironment, influencing progression. Our review delves into multifaceted role elucidating molecular crosstalk they orchestrate, their microRNA (miRNA) content. By shedding light nanoscale messengers, biogenesis, release, interaction uptake by target we aim deepen comprehension explore therapeutic potential, therefore increasing our AMD pathophysiology.

Language: Английский

Citations

13
Shared signaling pathways and comprehensive therapeutic approaches among diabetes complications DOI Creative Commons
Moein Ebrahimi, Hamid Ahmadieh, Mozhgan Rezaei Kanavi

et al.

Frontiers in Medicine, Journal Year: 2025, Volume and Issue: 11

Published: Jan. 8, 2025

The growing global prevalence of diabetes mellitus (DM), along with its associated complications, continues to rise. When clinically detected most DM complications are irreversible. It is therefore crucial detect and address these early systematically in order improve patient care outcomes. current clinical practice often prioritizes by addressing one complication while overlooking others that could occur. proposed the commonly targeted cell types including vascular cells, immune glial fibroblasts mediate might share responses diabetes. In addition, impact be influenced other complications. Recognizing focusing on shared among impacted cellular constituents, will allow simultaneously all DM-related limit adverse treatment impacts. This review explores understanding pathological signaling mechanisms recognizes new concepts benefit from further investigation both basic settings. ultimate goal develop more comprehensive strategies, which effectively multiple organs

Language: Английский

Citations

1
Advances in Extracellular-Vesicles-Based Diagnostic and Therapeutic Approaches for Ocular Diseases DOI Creative Commons
Yun Su,

Moxin Chen,

Wei Xu

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(34), P. 22793 - 22828

Published: Aug. 14, 2024

Extracellular vesicles (EVs) are nanoscale membrane of various sizes that can be secreted by most cells. EVs contain a diverse array cargo, including RNAs, lipids, proteins, and other molecules with functions intercellular communication, immune modulation, regulation physiological pathological processes. The biofluids in the eye, tears, aqueous humor, vitreous important sources for EV-based diagnosis ocular disease. Because molecular cargos may reflect biology their parental cells, these biofluids, as well blood, have been recognized promising candidates biomarkers early Moreover, also used therapeutics targeted drug delivery nanocarriers many disorders because low immunogenicity superior biocompatibility nature. In this review, we provide an overview recent advances field studies on We summarized origins applied disease, assessed different methods EV isolation from biofluid samples, highlighted bioengineering strategies systems, introduced latest applications treatment presented potential current clinical trials. Finally, briefly discussed challenges disease some issues concern better focusing translational future.

Language: Английский

Citations

6
Frontier role of extracellular vesicles in kidney disease DOI Creative Commons
Bei Li, Qi Chen, Yifan Zhang

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Sept. 20, 2024

Language: Английский

Citations

4
Analysis of preprocessing for generative adversarial networks: A case study on color fundoscopy to fluorescein angiography image-to-image translation DOI Creative Commons

Veena K.M,

Veena Mayya, Rashmi Naveen Raj

et al.

Computer Methods and Programs in Biomedicine Update, Journal Year: 2025, Volume and Issue: unknown, P. 100179 - 100179

Published: Jan. 1, 2025

Language: Английский

Citations

0
Engineered extracellular vesicles: an emerging nanomedicine therapeutic platform DOI

Jingshi Tang,

Dezhong Li, Rui Wang

et al.

Chemical Communications, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Extracellular vesicles have been deemed as potential drug carriers for treatment of various diseases. Recent advances summarized, including the sources, delivery function, extraction and cargo-loading technology extracellular vesicles.

Language: Английский

Citations

0
Retinal Biomarkers in Diabetic Retinopathy: From Early Detection to Personalized Treatment DOI Open Access

Georgios Chondrozoumakis,

Eleftherios Chatzimichail, Oussama Habra

et al.

Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(4), P. 1343 - 1343

Published: Feb. 18, 2025

Diabetic retinopathy (DR) is a leading cause of vision loss globally, with early detection and intervention critical to preventing severe outcomes. This narrative review examines the role retinal biomarkers-molecular imaging-in improving diagnosis, tracking disease progression, advancing personalized treatment for DR. Key biomarkers, such as inflammatory metabolic markers, imaging findings from optical coherence tomography fluorescence angiography genetic provide insights into mechanisms, help predict monitor responses treatments, like anti-VEGF corticosteroids. While challenges in standardization clinical integration remain, these biomarkers hold promise precision medicine approach that could transform DR management through early, individualized care.

Language: Английский

Citations

0
The Role of Natural Products in Diabetic Retinopathy DOI Creative Commons
Yuxuan Zhao, Yi Chen, Naihong Yan

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(6), P. 1138 - 1138

Published: May 21, 2024

Diabetic retinopathy (DR) is one of the most severe complications diabetes mellitus and potentially leads to significant visual impairment blindness. The complex mechanisms involved in pathological changes DR make it challenging achieve satisfactory outcomes with existing treatments. Diets conducive glycemic control have been shown improve diabetic patients, thus positioning dietary interventions as promising avenues for treatment. Investigations demonstrated that natural products (NPs) may effectively manage DR. Many types compounds, including saponins, phenols, terpenoids, flavonoids, saccharides, alkaloids, vitamins, exert anti-inflammatory, antioxidant, anti-neovascular, antiapoptotic effects vivo vitro. Nevertheless, clinical application NPs still faces challenges, such suboptimal specificity, poor bioavailability, a risk toxicity. Prospective studies are imperative validate therapeutic potential delaying or preventing

Language: Английский

Citations

2
Pro-Inflammatory Characteristics of Extracellular Vesicles in the Vitreous of Type 2 Diabetic Patients DOI Creative Commons
Shengshuai Shan,

Abdulaziz H. Alanazi,

Yohan Han

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(9), P. 2053 - 2053

Published: Sept. 10, 2024

Diabetic retinopathy (DR) is a leading cause of blindness, yet its molecular mechanisms are unclear. Extracellular vesicles (EVs) contribute to dysfunction in DR, but the characteristics and functions vitreous EVs This study investigated inflammatory properties type 2 diabetic (db) EVs. isolated from db non-db donors were used for nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), immunogold staining, Western blotting, proteomic by mass spectrometry. Intracellular uptake differentiated macrophages was evaluated using ExoGlow membrane labeling, impact on macrophage (THP-1) activation assessed cytokine levels RT-qPCR. NTA TEM showed non-aggregated with heterogeneous size range below 200 nm. blot detected EV markers (Alix, Annexin V, HSP70, Flotillin 1) an upregulation Cldn5 While incorporated into macrophages, treatment THP-1 cells significantly increased mRNA TNFα IL-1β compared Proteomic gene enrichment indicated pro-inflammatory Our results suggest potential involvement EC-derived Cldn5+ triggering inflammation, offering novel mechanism involved presenting possible therapeutic avenue DR.

Language: Английский

Citations

2