Comprehensive profiling of tsRNAs in acute coronary syndrome: expression patterns, clinical correlations, and functional insights

Human Genetics, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

Transfer RNA-derived small RNAs (tsRNAs) have emerged as potential biomarkers of various human diseases. However, the clinical utility and biological functions tsRNA in acute coronary syndrome (ACS) remain poorly understood. To investigate this, we performed high-throughput RNA sequencing on peripheral blood monocyte cells (PBMCs) from 24 ACS patients 12 healthy controls. Our analysis revealed distinct characteristic expression patterns tsRNAs response to ACS, highlighting their disease signatures PBMCs. Differentially expressed were validated using RT-qPCR two independent case-control sets. Among these, tRF-Gly-GCC-06 was significantly upregulated volunteers with unstable angina (UA) myocardial infarction (AMI) (p < 0.05) showed a statistically significant positive correlation Gensini score (r = 0.353, p 0.001). Moreover, this independently associated an increased risk after adjusting for conventional cardiovascular factors (odds ratio (OR) 1.58, 95% confidence interval (CI): 1.37-1.83, A series functional studies that facilitated macrophage proliferation migration modulated inflammation-related gene vitro. This study identified novel tRF-Gly-GCC-06, biomarker therapeutic target.

Language: Английский

tsRNA-00764 Regulates Estrogen and Progesterone Synthesis and Lipid Deposition by Targeting PPAR-γ in Duck Granulosa Cells

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 11251 - 11251

Published: Oct. 19, 2024

Transfer RNA-derived small RNAs (tsRNAs) are novel regulatory non-coding that have been found to modulate many life activities in recent years. However, the exact functions of tsRNAs follicle development remain unclear. Follicle is a remarkably complex process follows strict hierarchy and strongly associated with reproductive performance ducks. The converting yellow follicles into hierarchal known as selection, which directly determines number mature follicles. We performed RNA sequencing during selection ducks identified tsRNA-00764 target interest based on tsRNA expression profiles this study. Bioinformatics analyses luciferase reporter assays further revealed peroxisome proliferator-activated receptor-γ (PPAR-γ) was gene tsRNA-00764. Moreover, knockdown promoted estrogen progesterone synthesis lipid deposition duck granulosa cells, while PPAR-γ inhibitor reversed above phenomenon. Taken together, these results demonstrate tsRNA-00764, differentially expressed pre-hierarchal follicles, modulates by targeting serving potential mechanism selection. Overall, our findings provide theoretical foundation for exploration molecular mechanisms underlying production

Language: Английский

Mechanism and Therapeutic Targets of Circulating Immune Cells in Diabetic Retinopathy

Pharmacological Research, Journal Year: 2024, Volume and Issue: 210, P. 107505 - 107505

Published: Nov. 14, 2024

Diabetic retinopathy (DR) continues to be the leading cause of preventable vision loss among working-aged adults, marked by immune dysregulation within retinal microenvironment. Typically, retina is considered as an immune-privileged organ, where circulating cells are restricted from entry under normal conditions. However, during progression DR, this privilege compromised breach barrier and infiltrate retina. Increasing evidence suggests that vascular neuronal degeneration in DR largely driven infiltration cells, particularly neutrophils, monocyte-derived macrophages, lymphocytes. This review delves into mechanisms therapeutic targets associated with these cell populations offering a promising innovative approach managing disease.

Language: Английский