A connectome and analysis of the adult Drosophila central brain

eLife, Journal Year: 2020, Volume and Issue: 9

Published: Sept. 3, 2020

The neural circuits responsible for animal behavior remain largely unknown. We summarize new methods and present the circuitry of a large fraction brain fruit fly Drosophila melanogaster . Improved include procedures to prepare, image, align, segment, find synapses in, proofread such data sets. define cell types, refine computational compartments, provide an exhaustive atlas examples many them novel. detailed consisting neurons their chemical most central brain. make public simplify access, reducing effort needed answer circuit questions, linking defined by our analysis with genetic reagents. Biologically, we examine distributions connection strengths, motifs on different scales, electrical consequences compartmentalization, evidence that maximizing packing density is important criterion in evolution fly’s

Language: Английский

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A Complete Electron Microscopy Volume of the Brain of Adult Drosophila melanogaster

Cell, Journal Year: 2018, Volume and Issue: 174(3), P. 730 - 743.e22

Published: July 1, 2018

Drosophila melanogaster has a rich repertoire of innate and learned behaviors. Its 100,000-neuron brain is large but tractable target for comprehensive neural circuit mapping. Only electron microscopy (EM) enables complete, unbiased mapping synaptic connectivity; however, the fly too conventional EM. We developed custom high-throughput EM platform imaged entire an adult female at resolution. To validate dataset, we traced brain-spanning circuitry involving mushroom body (MB), which been extensively studied its role in learning. All inputs to Kenyon cells (KCs), intrinsic neurons MB, were mapped, revealing previously unknown cell type, postsynaptic partners KC dendrites, unexpected clustering olfactory projection neurons. These reconstructions show that this freely available volume supports circuits, will significantly accelerate neuroscience. VIDEO ABSTRACT.

Language: Английский

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The connectome of the adult Drosophila mushroom body provides insights into function

eLife, Journal Year: 2020, Volume and Issue: 9

Published: Dec. 14, 2020

Making inferences about the computations performed by neuronal circuits from synapse-level connectivity maps is an emerging opportunity in neuroscience. The mushroom body (MB) well positioned for developing and testing such approach due to its conserved architecture, recently completed dense connectome, extensive prior experimental studies of roles learning, memory, activity regulation. Here, we identify new components MB circuit Drosophila, including visual input output neurons (MBONs) with direct connections descending neurons. We find unexpected structure sensory inputs, transfer information different modalities MBONs, modulation that dopaminergic (DANs). provide insights into circuitry used integrate outputs, between central complex inputs DANs, feedback MBONs. Our results a foundation further theoretical work.

Language: Английский

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A connectome of the Drosophila central complex reveals network motifs suitable for flexible navigation and context-dependent action selection

eLife, Journal Year: 2021, Volume and Issue: 10

Published: Oct. 26, 2021

Flexible behaviors over long timescales are thought to engage recurrent neural networks in deep brain regions, which experimentally challenging study. In insects, circuit dynamics a region called the central complex (CX) enable directed locomotion, sleep, and context- experience-dependent spatial navigation. We describe first complete electron microscopy-based connectome of

Language: Английский

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Reconstruction of motor control circuits in adult Drosophila using automated transmission electron microscopy

Cell, Journal Year: 2021, Volume and Issue: 184(3), P. 759 - 774.e18

Published: Jan. 7, 2021

Language: Английский

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The natverse, a versatile toolbox for combining and analysing neuroanatomical data

eLife, Journal Year: 2020, Volume and Issue: 9

Published: April 14, 2020

To analyse neuron data at scale, neuroscientists expend substantial effort reading documentation, installing dependencies and moving between analysis visualisation environments. facilitate this, we have developed a suite of interoperable open-source R packages called the natverse. The natverse allows users to read local remote data, perform popular analyses including clustering graph-theoretic neuronal branching. Unlike most tools, enables comparison across many neurons morphology connectivity after imaging or co-registration within common template space. also transformations different spaces modalities. We demonstrate tools that integrate vast majority

Language: Английский

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Neurogenetic dissection of the Drosophila lateral horn reveals major outputs, diverse behavioural functions, and interactions with the mushroom body

eLife, Journal Year: 2019, Volume and Issue: 8

Published: May 21, 2019

Animals exhibit innate behaviours to a variety of sensory stimuli including olfactory cues. In Drosophila, one higher centre, the lateral horn (LH), is implicated in behaviour. However, our structural and functional understanding LH scant, large part due lack sparse neurogenetic tools for this region. We generate collection split-GAL4 driver lines providing genetic access 82 cell types. use these create an anatomical neurotransmitter map link EM connectomics data. find ~30% projections converge with outputs from mushroom body, site learning memory. Using optogenetic activation, we identify types that drive changes valence behavior or specific locomotor programs. summary, have generated resource manipulating mapping neurons, new insights into circuit basis learned behavior.

Language: Английский

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Optogenetic dissection of descending behavioral control in Drosophila

eLife, Journal Year: 2018, Volume and Issue: 7

Published: June 26, 2018

In most animals, the brain makes behavioral decisions that are transmitted by descending neurons to nerve cord circuitry produces behaviors. insects, only a few have been associated with specific To explore how control an insect’s movements, we developed novel method systematically assay effects of activating individual on freely behaving terrestrial D. melanogaster. We calculated two-dimensional representation entire behavior space explored these flies, and behaviors identifying regions this were visited increased frequency during optogenetic activation. Applying approach across large collection neurons, found (1) activation drove stereotyped behaviors, (2) in many cases multiple activated similar (3) optogenetically often dependent state prior

Language: Английский

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A single-cell transcriptomic atlas of the adult Drosophila ventral nerve cord

eLife, Journal Year: 2020, Volume and Issue: 9

Published: April 21, 2020

The Drosophila ventral nerve cord (VNC) receives and processes descending signals from the brain to produce a variety of coordinated locomotor outputs. It also integrates sensory information periphery sends ascending brain. We used single-cell transcriptomics generate an unbiased classification cellular diversity in VNC five-day old adult flies. produced atlas 26,000 high-quality cells, representing more than 100 transcriptionally distinct cell types. predominant gene signatures defining neuronal types reflect shared developmental histories based on neuroblast which cells were derived, as well their birth order. relative position along anterior-posterior axis could be assigned using Hox expression. This transcriptional fly will valuable resource for future studies neurodevelopment behavior.

Language: Английский

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Information flow, cell types and stereotypy in a full olfactory connectome

eLife, Journal Year: 2021, Volume and Issue: 10

Published: May 25, 2021

The

Language: Английский

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