iScience, Journal Year: 2024, Volume and Issue: 27(12), P. 111271 - 111271
Published: Oct. 28, 2024
Language: Английский
Journal of the Association for Research in Otolaryngology, Journal Year: 2019, Volume and Issue: 20(1), P. 1 - 19
Published: Jan. 11, 2019
Language: Английский
Citations
56
Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)
Published: Oct. 24, 2022
Otolith organs of the inner ear are innervated by two parallel afferent projections to brainstem and cerebellum. These innervations were proposed segregate across line polarity reversal (LPR) within each otolith organ, which divides organ into regions hair cells (HC) with opposite stereociliary orientation. The relationship functional significance these anatomical features not known. Here, we show regional expression Emx2 in organs, establishes LPR, mediates neuronal segregation LPR constitutes bidirectional sensitivity function. Conditional knockout (cKO) HCs lacks LPR. Tmie cKO, mechanotransduction was abolished selectively domain also sensitivity. Analyses both mutants indicate that is specifically required for mice swim comfortably traverse a balance beam efficiently, but stay on rotating rod.
Language: Английский
Citations
33
eLife, Journal Year: 2018, Volume and Issue: 7
Published: June 12, 2018
The lateral-line neuromast of the zebrafish displays a restricted, consistent pattern innervation that facilitates comparison microcircuits across individuals, developmental stages, and genotypes. We used serial blockface scanning electron microscopy to determine from multiple specimens connectome, comprehensive set connections between hair cells afferent efferent nerve fibers. This analysis delineated complex but wiring with three striking characteristics: each terminal is highly specific in receiving single directional sensitivity; redundant; terminals manifest hierarchy dominance. Mutation canonical planar-cell-polarity gene vangl2, which decouples asymmetric phenotypes sibling hair-cell pairs, results randomly positioned, oriented nonetheless retain wiring. Because larvae overexpress Notch exhibit uniformly oriented, innervating siblings, specificity mediated by signaling pathway.
Language: Английский
Citations
53
Nature Communications, Journal Year: 2019, Volume and Issue: 10(1)
Published: Sept. 5, 2019
Planar cell polarity (PCP) plays crucial roles in developmental processes such as gastrulation, neural tube closure and hearing. Wnt pathway mutants are often classified PCP due to similarities between their phenotypes. Here, we show that the zebrafish lateral line, disruptions of pathways have differential effects on hair orientations. While mutations genes vangl2 scrib cause random orientations cells, wnt11f1, gpc4 fzd7a/b induce cells adopt a concentric pattern. This pattern is not caused by defects but misaligned support cells. The molecular basis defect unknown demonstrate work parallel establish proper orientation. Consequently, orientation solely explained signaling, some phenotypes warrant re-evaluation.
Language: Английский
Citations
51
Journal of Neuroscience, Journal Year: 2020, Volume and Issue: 40(21), P. 4130 - 4144
Published: April 10, 2020
Information about water flow, detected by lateral line organs, is critical to the behavior and survival of fish amphibians. While certain aspects flow processing have been revealed through electrophysiology, we lack a comprehensive description neurons that respond network they form. Here, use brain-wide calcium imaging in combination with microfluidic stimulation map out, at cellular resolution, neuronal responses involved perceiving information larval zebrafish. We find diverse array responding head-to-tail (h-t) tail-to-head (t-h) or both. Early this pathway, ganglia, almost exclusively simple presence h-t t-h but later includes specifically onset, representing accumulated displacement during stimulus, encoding speed flow. The reporting on these more nuanced details are located across numerous brain regions, including some not previously implicated processing. A graph theory-based analysis shows majority dedicated detection, reinforced our finding like velocity total only encoded for direction. results represent first important modality, provide departure point detailed studies network. SIGNIFICANCE STATEMENT In aquatic animals, detecting stimuli, networks interpret remain mysterious. imaged activity individual entire brains zebrafish, revealing all response types their locations as occurs. others attuned direction, speed, duration has passed stimulus. With information, modeled underlying network, describing system its simulating motion rudimentary
Language: Английский
Citations
47
Neuroscience Letters, Journal Year: 2019, Volume and Issue: 709, P. 134373 - 134373
Published: July 8, 2019
Language: Английский
Citations
46
Current Biology, Journal Year: 2020, Volume and Issue: 30(6), P. 1142 - 1151.e6
Published: Feb. 27, 2020
Language: Английский
Citations
40
Current Biology, Journal Year: 2023, Volume and Issue: 33(7), P. 1295 - 1307.e3
Published: March 10, 2023
Language: Английский
Citations
15
Current topics in developmental biology/Current Topics in Developmental Biology, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Language: Английский
Citations
0
eLife, Journal Year: 2020, Volume and Issue: 9
Published: Dec. 30, 2020
Each hair cell (HC) precursor of zebrafish neuromasts divides to form two daughter HCs opposite bundle orientations. Previously, we showed that transcription factor Emx2, expressed in only one the HCs, generates this bidirectional HC pattern (Jiang et al., 2017). Here, asked whether Emx2 mediates effect by changing location establishment or positions since are known switch with each other. We rearrangement, redefined as processes named Rock and Roll, is required for positional acquisition HCs. Apical protrusion formation nascent planar polarity signaling both important Roll. facilitates Roll delaying apical its but it does not determine HCs’ ultimate positions, indicating where established
Language: Английский
Citations
24