Function and Evolution of Nuclear Receptors in Environmental-Dependent Postembryonic Development

Frontiers in Cell and Developmental Biology, Journal Year: 2021, Volume and Issue: 9

Published: June 10, 2021

Nuclear receptors (NRs) fulfill key roles in the coordination of postembryonal developmental transitions animal species. They control metamorphosis and sexual maturation virtually all animals by that two main environmental-dependent decision points. Sexual are controlled steroid thyroid receptors, respectively vertebrates, while both processes orchestrated ecdysone receptor (EcR) insects. The regulation these depends on environmental factors like nutrition, temperature, or photoperiods NRs form evolutionary conserved mediators phenotypic plasticity. While mechanism action for well studied model organisms, evolution systems is not entirely understood requires further investigation. We here review current knowledge NR involvement across tree life with special attention to integration signaling mechanism. Furthermore, we compare commonalities differences different systems. Finally, identify gaps our evolution, which, if sufficiently investigated, would lead an importantly improved understanding complex systems, history points, and, ultimately, speciation events metazoan kingdom.

Language: Английский

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Lipid Signaling During Gamete Maturation

Frontiers in Cell and Developmental Biology, Journal Year: 2022, Volume and Issue: 10

Published: June 24, 2022

Cell lipids are differentially distributed in distinct organelles and within the leaflets of bilayer. They can further form laterally defined sub-domains membranes with important signaling functions. This molecular spatial complexity offers optimal platforms for associated challenge dissecting these pathways especially that lipid metabolism tends to be highly interconnected. Lipid has historically been implicated gamete function, however detailed involved remain obscure. In this review we focus on oocyte sperm maturation an effort consolidate current knowledge role set stage future directions.

Language: Английский

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Relaxin-like Gonad-Stimulating Peptides in Asteroidea

Biomolecules, Journal Year: 2023, Volume and Issue: 13(5), P. 781 - 781

Published: April 30, 2023

Starfish relaxin-like gonad-stimulating peptide (RGP) is the first identified hormone with gonadotropin-like activity in invertebrates. RGP a heterodimeric peptide, comprising A and B chains disulfide cross-linkages. Although had been named substance (GSS), purified member of relaxin-type family. Thus, GSS was renamed as RGP. The cDNA encodes not only chains, but also signal C-peptides. After

Language: Английский

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A Putative Role of Vasopressin/Oxytocin-Type Neuropeptide in Osmoregulation and Feeding Inhibition of Apostichopus japonicus

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(18), P. 14358 - 14358

Published: Sept. 20, 2023

Vasopressin/oxytocin (VP/OT)-type neuropeptide is an ancient neurophysin-associated and has been intensively studied to be involved in multiple physiological processes protostomian deuterostome vertebrates. However, little known about the functions of VP/OT-type invertebrates especially echinoderms. Here, we firstly report signaling important economic species, Apostichopus japonicus, which widely cultured Asia, with high nutritional medicinal values. Molecular characterization analysis holotocin its precursor revealed highly conserved features VP/OT family. The candidate receptor for (AjHOR) was confirmed able activate via cAMP-PKA possible Ca2+-PKC pathway, further activated downstream ERK1/2 cascade. Holotocin expression profile showed that they were mainly concentrated circumoral nerve ring. Furthermore, vitro pharmacological experiments demonstrated caused contractile responses preparations from A. japonicus. And vivo functional studies indicated short-term injection resulted body bloat long-term reduced mass, suggesting potential roles osmoregulation feeding co-inhibition holotocin-CCK. Our findings provided a comprehensive description AjHOR-holotocin signaling, inhibition by controlling muscle contractions.

Language: Английский

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Metalloproteases in gonad formation and ovulation

General and Comparative Endocrinology, Journal Year: 2021, Volume and Issue: 314, P. 113924 - 113924

Published: Oct. 2, 2021

Language: Английский

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Transcriptomic Profiling Revealed Signaling Pathways Associated with the Spawning of Female Zebrafish under Cold Stress

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(14), P. 7494 - 7494

Published: July 6, 2022

As one of the critical abiotic factors, temperature controls fish development and reproduction. However, effects low on transcriptional regulation zebrafish reproduction remain largely unclear. In this study, fecundity was examined after exposure to cold temperatures at 19.5 °C, 19 18.5 or 18 °C. The °C showed no significant influence zebrafish, but significantly blocked spawning females, suggesting existence a point for females. Based these observations, brains anesthetized under stress different were collected high-throughput RNA-seq assays. Key genes, hub pathways important biological processes responding during identified through bioinformatic analysis. number down-regulated up-regulated genes reduction from egg-spawning non-spawning 2588 2527 (fold change ≥ 1.5 p-value ≤ 0.01), respectively. Venn analysis performed identify up- key genes. KEGG enrichment indicated that overrepresented among included GnRH signaling pathway, vascular smooth muscle contraction, C-type lectin receptor phosphatidylinositol system insulin pathway. GO revealed most inhibited photoreceptor cell outer segment organization, circadian gene expression maintenance. Furthermore, 99 hormone-related found in brain tissues groups, pathway enriched related hormones Thus, findings uncovered crucial controlling female stress.

Language: Английский

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Omics Studies for the Identification of Ascidian Peptides, Cognate Receptors, and Their Relevant Roles in Ovarian Follicular Development

Frontiers in Endocrinology, Journal Year: 2022, Volume and Issue: 13

Published: March 7, 2022

Omics studies contribute to the elucidation of genomes and profiles gene expression. In ascidian Ciona intestinalis Type A ( robusta ), mass spectrometry (MS)-based peptidomic have detected numerous -specific (nonhomologous) neuropeptides as well homologs typical vertebrate hypothalamic peptide hormones. Candidates for cognate G protein-coupled receptors (GPCRs) these peptides been found in transcriptome by two ways. First, homologous GPCRs counterparts sequence homology searches transcriptomes. Second, transcriptome-derived GPCR candidates used machine learning-based systematic prediction interactions not only between but also novel GPCRs. These data ultimately led experimental evidence various peptide-GPCR interactions. Comparative transcriptomics wildtype vasopressin (CiVP) gene-edited provide clues biological functions CiVP ovarian follicular development whole body growth. Furthermore, transcriptomes follicles treated with peptides, such tachykinin cionin (a cholecystokinin homolog), revealed key regulatory genes follicle growth, maturation, ovulation, eventually leading verification essential molecular mechanisms underlying events. findings indicate that omics studies, combined artificial intelligence single-cell technologies, pave way investigating greater details nervous, neuroendocrine, endocrine systems ascidians functional evolution diversity peptidergic networks throughout chordates.

Language: Английский

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Ciona spp. and ascidians as bioindicator organisms for evaluating effects of endocrine disrupting chemicals: A discussion paper

Marine Environmental Research, Journal Year: 2023, Volume and Issue: 191, P. 106170 - 106170

Published: Sept. 10, 2023

In context of testing, screening and monitoring endocrine-disrupting (ED) type environmental pollutants, tunicates could possibly represent a particularly interesting group bioindicator organisms. These primitive chordates are already important model organisms within developmental genomics research due to their central position in evolution close relationship vertebrates. The solitary ascidians, such as the genus Ciona spp. (vase tunicates), be extra feasible ED bioindicators. They have free-swimming, tadpole-like larval stage that develops extremely quickly (<20 h under favorable conditions), has short life cycle (typically 2–3 months), relatively easy maintain laboratory culture, fully sequenced genomes, transgenic embryos with 3D course data embryo ontogeny available. this article, we discuss possible roles (and other ascidians) ecotoxicological general but perhaps especially for effect studies contaminants presumed endocrine disrupting modes action.

Language: Английский

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Cionin, a vertebrate cholecystokinin/gastrin homolog, induces ovulation in the ascidian Ciona intestinalis type A

Scientific Reports, Journal Year: 2021, Volume and Issue: 11(1)

Published: May 25, 2021

Abstract Cionin is a homolog of vertebrate cholecystokinin/gastrin that has been identified in the ascidian Ciona intestinalis type A. The phylogenetic position ascidians as closest living relatives vertebrates suggests cionin can provide clues to evolution endocrine/neuroendocrine systems throughout chordates. Here, we show biological role regulation ovulation. In situ hybridization demonstrated mRNA receptor, Cior2 , was expressed specifically inner follicular cells pre-ovulatory follicles ovary. found significantly stimulate ovulation after 24-h incubation. Transcriptome and subsequent Real-time PCR analyses confirmed expression levels receptor tyrosine kinase (RTK) signaling genes matrix metalloproteinase (MMP) gene were elevated cionin-treated follicles. Of particular interest an RTK inhibitor MMP markedly suppressed stimulatory effect on Furthermore, inhibition reduced These results evidence induces by stimulating via pathway. This first report endogenous roles induction family peptides organism.

Language: Английский

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Vasopressin Promoter Transgenic and Vasopressin Gene-Edited Ascidian, Ciona intestinalis Type A (Ciona robusta): Innervation, Gene Expression Profiles, and Phenotypes

Frontiers in Endocrinology, Journal Year: 2021, Volume and Issue: 12

Published: May 6, 2021

Oxytocin (OT) and vasopressin (VP) superfamily neuropeptides are distributed in not only vertebrates but also diverse invertebrates. However, no VPergic innervation of invertebrates has ever been documented. In the ascidian,

Language: Английский

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