Information flow, cell types and stereotypy in a full olfactory connectome

eLife, Journal Year: 2021, Volume and Issue: 10

Published: May 25, 2021

The

Language: Английский

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Whole-brain annotation and multi-connectome cell typing of Drosophila

Nature, Journal Year: 2024, Volume and Issue: 634(8032), P. 139 - 152

Published: Oct. 2, 2024

Language: Английский

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Whole-brain annotation and multi-connectome cell typing quantifies circuit stereotypy inDrosophila

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 27, 2023

Abstract The fruit fly Drosophila melanogaster combines surprisingly sophisticated behaviour with a highly tractable nervous system. A large part of the fly’s success as model organism in modern neuroscience stems from concentration collaboratively generated molecular genetic and digital resources. As presented our FlyWire companion paper 1 , this now includes first full brain connectome an adult animal. Here we report systematic hierarchical annotation ∼130,000-neuron including neuronal classes, cell types developmental units (hemilineages). This enables any researcher to navigate huge dataset find systems neurons interest, linked literature through Virtual Fly Brain database 2 . Crucially, resource 4,552 types. 3,094 are rigorous consensus validations previously proposed “hemibrain” 3 In addition, propose 1,458 new types, arising mostly fact that spans whole brain, whereas hemibrain derives subvolume. Comparison showed type counts strong connections were largely stable, but connection weights variable within across animals. Further analysis defined simple heuristics for interpretation: stronger than 10 unitary synapses or providing >1% input target conserved. Some increased variability connectomes: most common mushroom body, required learning memory, is almost twice numerous hemibrain. We evidence functional homeostasis adjustments absolute amount excitatory while maintaining excitation-inhibition ratio. Finally, surprisingly, about one third could not yet be reliably identified connectome. therefore suggest should robust inter-individual variation, namely groups cells quantitatively more similar different other same brain. Joint connectomes demonstrates viability utility definition. Our work defines atlas provides both intellectual framework open source toolchain brain-scale comparative connectomics.

Language: Английский

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A Drosophila computational brain model reveals sensorimotor processing

Nature, Journal Year: 2024, Volume and Issue: 634(8032), P. 210 - 219

Published: Oct. 2, 2024

Language: Английский

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Descending neurons coordinate anterior grooming behavior in Drosophila

Current Biology, Journal Year: 2022, Volume and Issue: 32(4), P. 823 - 833.e4

Published: Feb. 1, 2022

The brain coordinates the movements that constitute behavior, but how descending neurons convey myriad of commands required to activate motor limbs in right order and combinations produce those is not well understood. For anterior grooming behavior fly, we show its component head sweeps leg rubs can be initiated separately, or as a set, by different neurons. Head are mutually exclusive front legs normally alternate, circuits ventral nerve cord resolve competing commands. Finally, left must work together remove debris. coordination for achieved unilateral activation single neuron, while similar manipulation neuron decouples single-sided sweeps. Taken together, these results demonstrate distinct orchestrate complex alternation between make up grooming.

Language: Английский

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Drosophila gustatory projections are segregated by taste modality and connectivity

eLife, Journal Year: 2022, Volume and Issue: 11

Published: May 25, 2022

Gustatory sensory neurons detect caloric and harmful compounds in potential food convey this information to the brain inform feeding decisions. To examine signals that gustatory transmit receive, we reconstructed axons their synaptic sites adult

Language: Английский

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The auditory efferent system in mosquitoes

Frontiers in Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 11

Published: Feb. 27, 2023

Whilst acoustic communication forms an integral component of the mating behavior many insect species, it is particularly crucial for disease-transmitting mosquitoes; swarming males rely on hearing faint sounds flying females courtship initiation. That can hear within din a swarm testament to their fabulous auditory systems. Mosquito highly frequency-selective, remarkably sensitive and, most strikingly, supported by elaborate system efferent neurons that modulate function - only documented example amongst insects. Peripheral release octopamine, serotonin and GABA appears differentially across major disease-carrying mosquito with receptors from other neurotransmitter families also identified in ears. Because relies flight tones partners, offers new potential targets control. It represents unique model studying networks. Here we review current knowledge system, briefly compare its counterparts species highlight future research directions unravel contribution perception.

Language: Английский

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Insect transient receptor potential vanilloid channels as potential targets of insecticides

Developmental & Comparative Immunology, Journal Year: 2023, Volume and Issue: 148, P. 104899 - 104899

Published: July 31, 2023

Language: Английский

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A leaky integrate-and-fire computational model based on the connectome of the entire adultDrosophilabrain reveals insights into sensorimotor processing

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: May 2, 2023

Abstract The forthcoming assembly of the adult Drosophila melanogaster central brain connectome, containing over 125,000 neurons and 50 million synaptic connections, provides a template for examining sensory processing throughout brain. Here, we create leaky integrate-and-fire computational model entire brain, based on neural connectivity neurotransmitter identity, to study circuit properties feeding grooming behaviors. We show that activation sugar-sensing or water-sensing gustatory in accurately predicts respond tastes are required initiation. Computational region those elicit motor neuron firing, testable hypothesis validate by optogenetic behavioral studies. Moreover, different classes makes accurate predictions how multiple taste modalities interact, providing circuit-level insight into aversive appetitive processing. Our sugar water pathways form partially shared initiation pathway, which our calcium imaging experiments confirm. Additionally, applied this mechanosensory circuits found small set comprising antennal do not overlap with circuits, describes response upon subtypes. results demonstrate modeling purely from predicted identity generates experimentally hypotheses can describe complete sensorimotor transformations.

Language: Английский

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Divergent neural circuits for proprioceptive and exteroceptive sensing of theDrosophilaleg

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 28, 2024

Abstract Somatosensory neurons provide the nervous system with information about mechanical forces originating inside and outside body. Here, we use connectomics from electron microscopy to reconstruct analyze neural circuits downstream of largest somatosensory organ in Drosophila leg, femoral chordotonal (FeCO). The FeCO has been proposed support both proprioceptive sensing fly’s femur-tibia joint exteroceptive substrate vibrations, but it was unknown which sensory central contribute each these functions. We found that different subtypes feed into distinct pathways. Position- movement-encoding connect local leg motor control ventral nerve cord (VNC), indicating a function. In contrast, signals vibration-encoding are integrated across legs transmitted mechanosensory regions brain, an Overall, our analyses reveal structure specialized for processing fly leg. These findings consistent growing body work invertebrate vertebrate species demonstrating existence limb pathways external vibrations.

Language: Английский

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