Chronic Alcohol Drinking Drives Sex-Specific Differences in Affective Behavior and Medial Prefrontal Cortex Activity in CRF1:Cre:Tdtomato Transgenic Rats DOI Creative Commons
Sema G. Quadir,

GM Arleth,

MG Cone

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Nov. 14, 2022

ABSTRACT Alcohol use disorders (AUDs) are characterized by compulsive alcohol use, loss of control over intake, and a negative emotional state during abstinence. While AUDs associated with both mood chronic pain disorders, the relationship between these associations remains unclear. Corticotropin releasing factor-1 receptor (CRF1) has been implicated in (EtOH) affective states, sensitivity; often sex-dependent manner. Using CRF1-cre transgenic rats, we found no sex differences basal behavior exception mechanical sensitivity, where females were more sensitive to stimuli. Following baseline testing, rats began EtOH (or water) drinking under intermittent access conditions. Females consumed first week, but overall intake was not significantly different males females. 3-4 weeks drinking, tested again for affect. decreased other group effects observed. However, individual directly correlated anxiety- depressive-like sexes. Interestingly, inversely thermal sensitivity only. There CRF1+ neuronal activity either prelimbic or infralimbic cortices, final session neurons cortex. Together, our results suggest complex interplay state, role prefrontal cortex CRF1-containing mediating behaviors. Additionally, highlight importance examining AUD-related SIGNIFICANCE STATEMENT Despite being extremely comorbid there is still limited understanding interaction directionality them. To investigate this problem, before after allowed drink 6 weeks. did predict subsequent predicted behavior. These findings accompanied increased corticotropin factor 1 containing region reveal new mechanism use.

Language: Английский

Distinct populations of corticotropin-releasing factor (CRF) neurons mediate divergent yet complementary defensive behaviors in response to a threat DOI Creative Commons

Rachel Chudoba,

Joanna Dabrowska

Neuropharmacology, Journal Year: 2023, Volume and Issue: 228, P. 109461 - 109461

Published: Feb. 10, 2023

Language: Английский

Citations

15
Activation of CRF/CRFR1 Signaling in the Central Nucleus of the Amygdala Contributes to Chronic Stress-Induced Exacerbation of Neuropathic Pain by Enhancing GluN2B-NMDA Receptor-Mediated Synaptic Plasticity in Adult Male Rats DOI
Yue Tian,

Xuewei Yang,

Lin Chen

et al.

Journal of Pain, Journal Year: 2024, Volume and Issue: 25(8), P. 104495 - 104495

Published: Feb. 12, 2024

Language: Английский

Citations

5
Lycium barbarum polysaccharide ameliorates corticosterone-induced cognition decline with modulation of CRHR1 DOI Creative Commons
Xiaoxiao Shi, Xiaofeng Tian, Bin He

et al.

Brain Research Bulletin, Journal Year: 2025, Volume and Issue: unknown, P. 111346 - 111346

Published: April 1, 2025

Lycium barbarum polysaccharide (LBP) has anti-inflammatory, anti-oxidation and anti-aging properties, but the mechanism of LBP on stress-induced cognitive dysfunction caused by elevated GC level is still unclear. Therefore, present study aimed to investigate corticosterone-injected（CORT-injected） impairment. The rat model was induced corticosterone in vivo. Water maze test HE staining were used observe effect function brain morphology CORT-injected rats. RT-qPCR, Western blot, immunofluorescence detect expression proteins. SH-SY5Y cells treated with CORT D-gal vitro, respectively. cell proliferation observed, western blotting detected protein expressions. In this study, treatment ameliorated CORT-induced learning protected hypothalamic hippocampal neurons from injury addition, reduced plasma concentrations results also indicated that enhanced synapsis-related proteins PSD95 SYN up-regulating CRHR1 RGS2 hippocampus hypothalamus group. Meanwhile, we confirmed - D-Gal-induced further verified changes CRHR1, RGS2, synapse-related This demonstrates cognition decline regulating CRHR1. may represent a potential drug for prevention patients increased GCs.

Language: Английский

Citations

0
Corticotropin-Releasing Hormone Neurons in the Central Amygdala Nucleus: An Integrative Hub for Pain, Emotion, and Addiction Neurobiology DOI
Jia Li, Ziang Li, Haifeng Tian

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Long‐lasting mechanical hypersensitivity and CRF receptor type‐1 neuron activation in the BNST following adolescent ethanol exposure DOI

Natalia Bonetti Bertagna,

Lisa Wilson,

Connor K. Bailey

et al.

Alcohol Clinical and Experimental Research, Journal Year: 2023, Volume and Issue: 48(1), P. 48 - 57

Published: Nov. 14, 2023

Adolescent alcohol use can produce long-lasting alterations in brain function, potentially leading to adverse health outcomes adulthood. Emerging evidence suggests that chronic increase pain sensitivity or exacerbate existing conditions, but the potential neural mechanisms underlying these effects require further investigation. Here, we evaluate impact of ethanol vapor on mechanical over course acute and protracted withdrawal adolescent adult male female mice, its association with corticotropin-releasing factor (CRF) signaling within bed nucleus stria terminalis (BNST).

Language: Английский

Citations

7
Traumatic Stress-Induced Increases in Anxiety-like Behavior and Alcohol Self-Administration Are Mediated by Central Amygdala CRF1 Neurons That Project to the Lateral Hypothalamus DOI Creative Commons
Marcus M. Weera,

Daniel A. Webster,

Rosetta S. Shackett

et al.

Journal of Neuroscience, Journal Year: 2023, Volume and Issue: 43(50), P. 8690 - 8699

Published: Nov. 6, 2023

Avoidance stress coping, defined as persistent internal and/or external avoidance of stress-related stimuli, is a key feature anxiety- and disorders, contributes to increases in alcohol misuse after exposure. Previous work using rat model predator odor identified corticotropin-releasing factor (CRF) signaling via CRF Type 1 receptors (CRF1) the CeA, well CeA projections lateral hypothalamus (LH) mediators conditioned stress-paired contexts increased drinking stress. Here, we report that CRF1-expressing cells project LH are preferentially activated male female rats show (termed Avoider rats), chemogenetic inhibition these rescues stress-induced anxiety-like behavior self-administration rats. Using slice electrophysiology, found prior exposure blunts inhibitory synaptic transmission drive CRF1 CeA-LH cells. In addition, bath application reduces Non-Avoiders only. Collectively, data contribute SIGNIFICANCE STATEMENT Stress may lead variety behavioral physiological negative consequences, better understanding neurobiological mechanisms effects improved prevention treatment strategies. This study, performed laboratory rats, shows animals exhibit coping go on develop heightened self-administration, behaviors can be rescued by inhibiting activity specific population neurons central amygdala. study also describes changes their role promoting anxiety self-administration.

Language: Английский

Citations

5
Psychedelic compounds directly excite 5-HT2ALayer 5 Pyramidal Neurons in the Prefrontal Cortex through a 5-HT2AGq -mediated activation mechanism DOI Open Access

Gavin P. Schmitz,

Yi-Ting Chiu,

Gabriele M. König

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Nov. 15, 2022

Summary Psilocin, the active compound in Psilocybe sp . mushrooms, is a serotonergic psychedelic that has recently gained renewed interest due to its potential as therapeutic tool. Despite promising clinical findings, underlying signaling mechanisms and brain region-specific effects of psilocin other drugs remain unclear. like compounds, an agonist at many serotonin biogenic amine receptors; however, activation (5-Hydroxytryptamine, or 5-HT) 2A receptors (5-HT Rs) understood main molecular target for psychoactive animals humans. 5-HT Rs are abundantly expressed prefrontal cortex (PFC); biochemical actions on PFC neurons poorly understood. In this study, we used vitro slice electrophysiology examine how acutely alters activity electrophysiological properties layer 5 pyramidal mouse PFC. Focal application (10 μ M) onto nonspecified Layer Pyramidal prelimbic C57BL/6J mice produced variable firing (increase, decrease, no change). R were identified via labeling 5-HT2A-ERT2-Cre crossed with Ai9 tdTomato reporter. increased all but did not result any significant changes synaptic transmission. Overall, results demonstrate evokes strong G α q dependent, thereby providing valuable insights into region implicated mediating drug actions.

Language: Английский

Citations

9
Chronic Alcohol Drinking Drives Sex-Specific Differences in Affective Behavior and Medial Prefrontal Cortex Activity in CRF1:Cre:tdTomato Transgenic Rats DOI Creative Commons
Sema G. Quadir,

Gillian M. Arleth,

M. G. Cone

et al.

eNeuro, Journal Year: 2023, Volume and Issue: 10(7), P. ENEURO.0055 - 23.2023

Published: July 1, 2023

In 2021, 131 million adult Americans reported drinking alcohol in the last month, despite well-known consequences of consumption. While use disorders (AUDs) are associated with both mood and chronic pain disorders, relationship between affective nociceptive behaviors remains unclear. Corticotropin releasing factor receptor-1 (CRF1) has been implicated drinking, states, sensitivity, often a sex-dependent manner. order to probe effects on activity CRF1+ cells also test hypothesis that is basal subsequent readouts, we put male female CRF1:cre:tdTomato rats through battery behavioral tests before after intermittent access alcohol. Following baseline testing, began (or water) drinking. Females consumed more first week, but there was no effect sex overall intake. three four weeks were repeated. Alcohol decreased mechanical other observed experimental groups. Individual intake correlated behavior sexes only thermal sensitivity males. There main or neuronal medial prefrontal cortex (PFC) final session neurons infralimbic (IL) subregion. Together, our results suggest complex interplay state, role mediating these behaviors.

Language: Английский

Citations

4
Divergent Population-Specific Effects of Chronic Ethanol Exposure on Excitability and Inhibitory Transmission in Male and Female Rat Central Amygdala DOI Creative Commons
Elizabeth J. Crofton, Todd K. O’Buckley, John Bohnsack

et al.

Journal of Neuroscience, Journal Year: 2023, Volume and Issue: 43(42), P. 7056 - 7068

Published: Sept. 1, 2023

The central nucleus of the amygdala (CeA) is implicated in alcohol use disorder (AUD) and AUD-associated plasticity. CeA a primarily GABAergic that subdivided into lateral medial compartments with genetically diverse subpopulations. GABA A receptors are heteromeric pentamers subunits conferring distinct physiological characteristics. receptor signaling has been ethanol-associated plasticity; however, population-specific changes inhibitory subunit expression remain unclear. Here, we combined electrophysiology single-cell gene analysis population markers to examine control male female rats following chronic ethanol exposure. We found exposure withdrawal produced global at transcript protein levels, increased excitability neurons, synaptic transmission neurons. When examined neurons level heterogenous populations, as previously reported. observed ethanol-induced increases only somatostatin females, decreases kinase C delta (PKCd) males, PKCd calbindin 2-expressing There were no differences (Gabr) males but reduced GabrA5 Collectively, these findings suggest defined populations display differential sensitivity which may play role sex vulnerability AUD or pathology. SIGNIFICANCE STATEMENT involved effects brain; activity used recordings neuronal sex- withdrawal. Specifically, displayed population, whereas both decreased population. These identify exposure, contribute disorder.

Language: Английский

Citations

4
Amygdala AVPR1A mediates susceptibility to chronic social isolation in females DOI Open Access
Marie François,

Isabella Canal Delgado,

Alexandre Lafond

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Feb. 15, 2023

Summary Females are more sensitive to social exclusion, which could contribute their heightened susceptibility anxiety disorders. Chronic isolation stress (CSIS) for at least 7 weeks after puberty induces anxiety-related behavioral adaptations in female mice. Here, we show that Arginine vasopressin receptor 1a ( Avpr1a )-expressing neurons the central nucleus of amygdala (CeA) mediate these sex-specific effects, part, via projections caudate putamen. Loss function studies demonstrate AVPR1A signaling CeA is required effects CSIS on behaviors females but has no effect males or group housed females. This sex-specificity mediated by AVP produced a subpopulation posterodorsal medial project CeA. Estrogen alpha also contributes preferential sensitivity CSIS. These data support new therapeutic applications antagonists women.

Language: Английский

Citations

3