A core of functional complementary bacteria infects oysters in Pacific Oyster Mortality Syndrome DOI Creative Commons
Camille Clérissi, Xing Luo,

Aude Lucasson

et al.

Animal Microbiome, Journal Year: 2023, Volume and Issue: 5(1)

Published: May 3, 2023

Abstract Background The Pacific oyster Crassostrea gigas is one of the main cultivated invertebrate species worldwide. Since 2008, juveniles have been confronted with a lethal syndrome known as Oyster Mortality Syndrome (POMS). POMS polymicrobial disease initiated by primary infection herpesvirus OsHV-1 µVar that creates an immunocompromised state and evolves towards secondary fatal bacteremia. Results In present article, we describe implementation unprecedented combination metabarcoding metatranscriptomic approaches to show sequence events in pathogenesis conserved across infectious environments. We also identified core bacterial consortium which, together µVar, forms pathobiota. This characterized high transcriptional activities complementary metabolic functions exploit host’s resources. A significant specificity was highlighted at genus level, suggesting low competition for nutrients between members bacteria. Conclusions Lack bacteria might favor colonization host tissues contribute conservation pathobiota distinct

Language: Английский

Micro”bee”ota: Honey Bee Normal Microbiota as a Part of Superorganism DOI Creative Commons
Daniil Smutin, Egor Lebedev,

Maxim Selitskiy

et al.

Microorganisms, Journal Year: 2022, Volume and Issue: 10(12), P. 2359 - 2359

Published: Nov. 29, 2022

Honey bees are model organisms for microbiota research. Gut microbiomes very interesting surveys due to their simple structure and relationship with hive production. Long-term studies reveal the gut patterns of various members, as well functions, sources, interactions majority its bacteria. But fungal non-pathogenic part is almost unexplored, likewise some other related microbiota. bees, superorganisms, interact own microorganisms, microbial communities food stores, surfaces, environments. Understanding diversity, transition ways, niche colonization control necessary understanding any separate because interplay. The long coevolution microorganisms populating these niches makes systems co-dependent, integrated, stable. Interaction environment, hive, determines caste lifestyle individual In this article, we bring together on western honey bee. We show a possible between determination composition. And what primary: differentiation or composition?

Language: Английский

Citations

32
Microbial interactions in theory and practice: when are measurements compatible with models? DOI Creative Commons
Aurore Picot, Shota Shibasaki, Oliver J. Meacock

et al.

Current Opinion in Microbiology, Journal Year: 2023, Volume and Issue: 75, P. 102354 - 102354

Published: July 6, 2023

Most predictive models of ecosystem dynamics are based on interactions between organisms: their influence each other’s growth and death. We review here how theoretical approaches used to extract interaction measurements from experimental data in microbiology, particularly focusing the generalised Lotka–Volterra (gLV) framework. Though widely used, we argue that gLV model should be avoided for estimating batch culture — most common, simplest cheapest vitro approach culturing microbes. Fortunately, alternative offer a way out this conundrum. Firstly, side, alternatives such as serial-transfer chemostat systems more closely match assumptions model. Secondly, explicit organism-environment can study batch-culture systems. hope our recommendations will increase tractability microbial experimentalists theoreticians alike.

Language: Английский

Citations

23
Deep Divergence and Genomic Diversification of Gut Symbionts of Neotropical Stingless Bees DOI Creative Commons
Garance Sarton-Lohéac, Carlos Gustavo Nunes-Silva, Florent Mazel

et al.

mBio, Journal Year: 2023, Volume and Issue: 14(2)

Published: March 20, 2023

Social bees harbor conserved gut microbiotas that may have been acquired in a common ancestor of social and subsequently codiversified with their hosts. However, most this knowledge is based on studies the honey bumblebees. Much less known about third diverse group bees, stingless bees. Specifically, absence genomic data from presents an important gap understanding evolution functional diversity bee microbiota. Here, we combined community profiling culturing genome sequencing bacteria six neotropical species Brazil. Phylogenomic analyses show isolates form deep-branching sister clades core members bumblebee microbiota capabilities, confirming ancestry ecology our bacterial phylogenies were not congruent those host, indicating was driven by strict codiversification but included host switches independent symbiont gain losses. Finally, as reported for microbiotas, found substantial divergence among strains bacteria, suggesting adaptation to different glycan niches. Our study offers first insights into highlights need broader samplings understand IMPORTANCE Stingless are corbiculate represent pollinator throughout tropics subtropics. They specialized microbial communities related bumblebees likely health. Few cultured which has prevented characterization potential. established cultures major sequenced genomes. We belong novel distantly encoding similar capabilities. new perspective basis characterizing symbiotic relationships between

Language: Английский

Citations

21
Microbes, the ‘silent third partners’ of bee–angiosperm mutualisms DOI
Shawn A. Steffan, Prarthana S. Dharampal, Jordan G. Kueneman

et al.

Trends in Ecology & Evolution, Journal Year: 2023, Volume and Issue: 39(1), P. 65 - 77

Published: Nov. 6, 2023

Language: Английский

Citations

21
Atrazine exposure can dysregulate the immune system and increase the susceptibility against pathogens in honeybees in a dose-dependent manner DOI
Kang Wang, Minqi Cai, Sun Jie

et al.

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 452, P. 131179 - 131179

Published: March 10, 2023

Language: Английский

Citations

20
Low-level resource partitioning supports coexistence among functionally redundant bacteria during successional dynamics DOI Creative Commons
Xiaoqian Yu, Craig McLean, Jan‐Hendrik Hehemann

et al.

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

Abstract Members of microbial communities can substantially overlap in substrate use. However, what enables functionally redundant microorganisms to coassemble or even stably coexist remains poorly understood. Here, we show that during unstable successional dynamics on complex, natural organic matter, bacteria by partitioning low-concentration substrates though they compete for one simple, dominant substrate. We allowed ocean self-assemble leachates the brown seaweed Fucus vesiculosus and then analyzed competition among 10 taxonomically diverse isolates representing two distinct stages succession. All, but isolates, exhibited an average 90% ± 6% pairwise resource use, functional redundancy from same assembly stage was higher than between stages, leading us construct a simpler four-isolate community with each early late stages. found that, although short-term F. leachate dependent initial isolate ratios, long term, four leachate, albeit some strains at low abundance. therefore explored potential nonredundant use genomic content analysis RNA expression patterns. This revealed mainly differed peripheral metabolic pathways, such as ability degrade pyrimidine, leucine, tyrosine, well aromatic substrates. These results highlight importance fine-scale differences strategies supporting frequently observed coexistence large numbers rare organisms microbiomes.

Language: Английский

Citations

9
The importance of host physical niches for the stability of gut microbiome composition DOI Creative Commons
William B. Ludington

Philosophical Transactions of the Royal Society B Biological Sciences, Journal Year: 2024, Volume and Issue: 379(1901)

Published: March 18, 2024

Gut bacteria are prevalent throughout the Metazoa and form complex microbial communities associated with food breakdown, nutrient provision disease prevention. How hosts acquire maintain a consistent bacterial flora remains mysterious even in best-studied animals, including humans, mice, fishes, squid, bugs, worms flies. This essay visits evidence that have co-evolved relationships specific some of these supported by specialized physical niches select, sequester symbionts. Genetics approaches could uncover mechanisms for recruiting maintaining stable members microbiome. article is part theme issue 'Sculpting microbiome: how host factors determine respond to colonization'.

Language: Английский

Citations

9
Insights into fermentation with lactic acid bacteria on the flavonoids biotransformation of alfalfa silage DOI Creative Commons
Yu Gao,

Hongzhang Zhou,

Yuan Wang

et al.

Chemical and Biological Technologies in Agriculture, Journal Year: 2024, Volume and Issue: 11(1)

Published: May 21, 2024

Abstract Background Oxidative stress is currently one of the main threats to animal health, and flavonoids in forage have good antioxidant activity. However, impact fermentation on their activity still unclear. This study aims investigate effect lactic acid bacteria inoculation biological transformation alfalfa silage its relationship with Results Compared raw materials, can increase total flavonoid content alfalfa. The addition Pediococcus pentosaceus (CP115739.1) Lactiplantibacillus plantarum (CP115741.1) significantly ( P < 0.05). improved capacity Pearson correlation analysis showed a significant between DPPH (R = 0.62, 0.05), highly FRAP 0.70, 0.01). natural fermentation, leads changes process Its unique products, 3,7,4′-trioxyflavonoids, as well acacetin taxifolin 7-O-rhamnoside, are positively correlated Conclusions Silage contributes flavonoids, certain (including apigenin, luteolin, other free flavonoids). It worth noting that after improved, which may be attributed biotransformation related acacetin, 3,7,4′-trihydroxyflavonoids, 7-O-rhamnoside. provides potential pathway for obtaining value-added products by selecting specific inoculants. Graphical

Language: Английский

Citations

9
Synergistic pectin deconstruction is a prerequisite for mutualistic interactions between honeybee gut bacteria DOI Creative Commons
Junbo Tang, Wenlong Zuo,

Lizhen Guo

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Aug. 13, 2024

The honeybee gut microbiome is crucial for degrading diverse pollen glycans. Yet it unclear how this process shapes the interactions among bacteria. Here, we demonstrate a conditional mutualistic interaction between strains of two bacteria Bifidobacterium asteroides and Gilliamella apicola. When co-occurring in vitro vivo, provides complementary demethylation service to promote growth on methylated homogalacturonan, an enriched polysaccharide pectin. In exchange, shares digestive products with Bifidobacterium, through which positive established. This vanishes when not required non-methylated diet. Results from biochemical gene expression analyses combined model simulation further suggest that ratio change major homogalacturonan breakdown products, galacturonic acid (GalA) di-GalA, determines bacterial interaction. study unravels glycan metabolism may shape Microbial honey bee are incompletely understood. authors report reciprocal core genera: aids pectin backbone enabling its by Gilliamella; return, digestion products.

Language: Английский

Citations

9
Growth of complete ammonia oxidizers on guanidine DOI Creative Commons
Márton Palatinszky, Craig W. Herbold, Christopher J. Sedlacek

et al.

Nature, Journal Year: 2024, Volume and Issue: 633(8030), P. 646 - 653

Published: Aug. 14, 2024

Guanidine is a chemically stable nitrogen compound that excreted in human urine and widely used manufacturing of plastics, as flame retardant component propellants, well known protein denaturant biochemistry

Language: Английский

Citations

9