Global status of gene edited animals for agricultural applications

The Veterinary Journal, Journal Year: 2024, Volume and Issue: 305, P. 106142 - 106142

Published: May 22, 2024

Gene editing (GnEd) involves using a site-directed nuclease to introduce double-strand break (DSB) at targeted location in the genome. A literature search was performed on use of GnEd animals for agricultural applications. Data extracted from 212 peer-reviewed articles that described production least one living animal employing technologies purposes. The most common system reported CRISPR/Cas9, and frequent type edit unguided insertion or deletion resulting repair DSB leading knock-out (KO) mutation. Animal groups included reviewed papers were ruminants (cattle, sheep, goats, n=63); monogastrics (pigs rabbit, n=60); avian (chicken, duck, quail, n=17); aquatic (many species, n=65), insects (honeybee, silkworm, n=7). Yield (32%), followed by reproduction (21%) disease resistance (17%) most- commonly traits. Over half had Chinese first-authorship. Several countries, including Argentina, Australia, Brazil, Colombia Japan, have adopted regulatory policy considers KO mutations introduced following as akin natural genetic variation, therefore treat these analogously those produced conventional breeding. This approach has resulted non-GMO determination small number food applications, three species fast-growing fish, (red sea bream, olive flounder tiger pufferfish Japan), fish cattle Argentina porcine reproductive respiratory syndrome (PRRS) virus disease-resistant pigs Colombia.

Language: Английский

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Fourth Report on Chicken Genes and Chromosomes 2022

Cytogenetic and Genome Research, Journal Year: 2022, Volume and Issue: 162(8-9), P. 405 - 528

Published: Jan. 1, 2022

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Language: Английский

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Poultry genetic heritage cryopreservation and reconstruction: advancement and future challenges

Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Journal Year: 2022, Volume and Issue: 13(1)

Published: Oct. 9, 2022

Abstract Poultry genetics resources, including commercial selected lines, indigenous breeds, and experimental are now being irreversibly lost at an alarming rate due to multiple reasons, which further threats the future livelihood academic purpose. Collections of germplasm may reduce risk catastrophic loss genetic diversity by guaranteeing that a pool variability is available ensure reintroduction replenishment stocks. The setting up biobanks for poultry challenging because high sensitiveness spermatozoa freezing–thawing process, inability cryopreserve egg or embryo, coupled with females heterogametic sex. progress in cryobiology biotechnologies have made possible extension range species cryobanks, semen, primordial germ cells, somatic cells gonads. In this review, we introduce state-of-the-art technologies avian resource conservation breed reconstruction, discuss potential challenges study extending these ongoing efforts.

Language: Английский

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Cloning for the Twenty-First Century and Its Place in Endangered Species Conservation

Annual Review of Animal Biosciences, Journal Year: 2023, Volume and Issue: 12(1), P. 91 - 112

Published: Nov. 21, 2023

Cloning as it relates to the animal kingdom generally refers production of genetically identical individuals. Because cloning is increasingly subject renewed attention a tool for rescuing endangered or extinct species, seems timely dissect role numerous reproductive techniques encompassed by this term in species conservation. Although typically associated with somatic cell nuclear transfer, recent advent additional that allow genome replication without genetic recombination demands use induced pluripotent stem cells generate gametes embryos, well older methods such embryo splitting, all be included discussion. Additionally, phenomenon natural (e.g., subset fish, birds, invertebrates, and reptilian reproduce via parthenogenesis) must also pointed out. Beyond biology these are practical considerations ethics using procedures species. All examined concert determine whether has place Therefore, we synthesize progress ethical aspects they pertain

Language: Английский

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Avian Bioresources for Developmental Biology: Chicken and Quail Resources in the United Kingdom, France, and Japan.

Developmental Biology, Journal Year: 2025, Volume and Issue: 521, P. 1 - 13

Published: Feb. 2, 2025

Language: Английский

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Innovations in poultry reproduction using cryopreserved avian germ cells

Reproduction in Domestic Animals, Journal Year: 2024, Volume and Issue: 59(5)

Published: May 1, 2024

Meat and eggs from chicken are the major source of animal protein for human population. The cryopreservation poultry species is needed to guarantee sustainable production. Here, we describe existing technologies avian reproductive cells using embryonic germ cells, spermatozoa ovarian tissues. We outline strategies reconstitute breeds their cryopreserved surrogate hosts discuss perspectives genetic conservation reconstitution wild host animals.

Language: Английский

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Successful xenotransplantation of testicular cells following fractionated chemotherapy of recipient birds

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Feb. 7, 2024

Abstract An essential step in the success of germ cell transplantation is preparation recipient’s testicular environment to increase availability stem niches. However, most methods for this purpose birds face serious limitations such as partial depletion, high toxicity and mortality, or need use expensive technologies. Here, we validated a simple practical technique transferring quail cells into chicken testes depleted endogenous spermatozoa by fractioned chemotherapy (20 mg/kg/week busulfan 5 weeks). This protocol resulted very low mortality treated day-old chicks and, despite maintenance androgenic activity, sperm production was decreased 84.3% at 25 weeks age. NANOG immunostaining revealed that few no were present following treatment with 20 40 mg/kg, respectively. RT-qPCR data also showed c-MYC expression declined these treatments, but GRFα1 BID expressions remained unaltered among groups. After xenotransplantation, immunodetected using species-specific antibody (QCPN), ovalbumin DNA found seminal samples collected from recipients. Together, confirm fractionated administration hatchlings practical, effective, safe prepare recipient male capable supporting xenogeneic spermatogenesis.

Language: Английский

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Avian iPSC Derivation to Recover Threatened Wild Species: A Comprehensive Review in Light of Well-Established Protocols

Animals, Journal Year: 2024, Volume and Issue: 14(2), P. 220 - 220

Published: Jan. 10, 2024

Induced pluripotent stem cells (iPSCs) were first generated by Yamanaka in 2006, revolutionizing research overcoming limitations imposed the use of embryonic cells. In terms conservation endangered species, iPSC technology presents itself as a viable alternative for manipulation target genetics without compromising specimens. Although iPSCs have been successfully various their application nonmammalian particularly avian requires further in-depth investigation to cover diversity wild species at risk and different protocol requirements. This study aims provide an overview workflow induction, comparing well-established protocols humans mice with limited information available species. Here, we discuss somatic cell sources be reprogrammed, genetic factors, delivery methods, enhancers, brief history achievements derivation, main approaches characterization, future perspectives challenges field. By examining current state-of-the-art techniques employed generation, seek contribute development efficient species-specific methodologies at-risk The advancement holds great promise achieving vitro germline competency and, consequently, addressing reproductive providing valuable tools basic research, bird preservation rescue, establishment cryobanks efforts.

Language: Английский

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New Genomic Techniques (NGT) in animals and their agri/food/feed products

EFSA Supporting Publications, Journal Year: 2023, Volume and Issue: 20(9)

Published: Sept. 1, 2023

This report presents a review of the commercial and pre-commercial stage applications new genomic technologies (NGT) applied to farm animals their agri/food/feed products.Additionally, literature was performed compile comprehensive listing peerreviewed research development gene edited for food agricultural applications.A total 195 publications resulting in live were compiled.To date, several developed or ongoing have been authorized commerce, judged be "non-GMO" hence conventional, at least one country including knockout tiger pufferfish red sea bream Japan; tilapia, cattle, pigs horses Argentina; cattle tilapia Brazil; two gene-edited granted enforcement discretion United States meaning products can enter supply.One application, targeted exon deletion porcine respiratory reproductive syndrome virus resistance is formally precommercial stage.There are proof-of-concept multiple species testing targets traits interest.The most common trait category meat fibre yield (31%), followed by reproduction (24%), biotic stress (18%), (7%), colour (6%), production hypoallergenic (5%), product quality (4%), abiotic (1%), other (4%).The majority these SDN-1 using Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 introduce small insertions deletions inactivate gene.The large number focused on due part interest both single-sex offspring numerous industries (e.g.females case egg production), infertility coupled with germline complementation chimeras (where germline-competent donor cells used replace an otherwise sterile host different genetic background) finfish, chickens, goats, pigs.

Language: Английский

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Incorporation of Biotechnologies into Gene Banking Strategies to Facilitate Rapid Reconstitution of Populations

Animals, Journal Year: 2023, Volume and Issue: 13(20), P. 3169 - 3169

Published: Oct. 11, 2023

National animal gene banks that are responsible for conserving livestock, poultry, and aquatic genetic resources need to be capable of utilizing a broad array cryotechnologies coupled with assisted reproductive technologies reconstitute either specific animals or populations/breeds as needed. This capability is predicated upon having sufficient diversity (usually encapsulated by number in the collection), units germplasm tissues, ability animals. While Food Agriculture Organization United Nations (FAO 2012, 2023) developed set guidelines on these matters, those do not consider applications utilization newer (e.g., primordial germ cells, cloning from somatic embryo transfer, IVF, sex-sorted semen), which can radically change how collect, store, utilize resources. paper reviews current status using technologies, explores might make such part their routine operations, illustrates combining older approaches enables populations reconstituted more efficiently.

Language: Английский

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