bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Aug. 22, 2023
Ovulation
is
essential
for
reproductive
success,
yet
the
underlying
cellular
and
molecular
mechanisms
are
far
from
clear.
Here,
we
applied
high-resolution
spatiotemporal
transcriptomics
to
map
out
cell-type-
ovulation-stage-specific
programs
as
function
of
time
during
follicle
maturation
ovulation
in
mice.
Our
analysis
revealed
dynamic
transitions
within
granulosa
cell
types
that
occur
tight
coordination
with
mesenchymal
proliferation.
We
identified
new
markers
emerging
cumulus
fate
preantral-to-antral
transition.
describe
transcriptional
respond
rapidly
stimulation
those
associated
rupture,
highlighting
prominent
roles
apoptotic
metabolic
pathways
final
stages
maturation.
further
report
stage-specific
oocyte-cumulus
interactions
diverging
differentiation
follicles
approaching
ovulation.
Collectively,
this
study
provides
insights
into
processes
regulate
mouse
ovarian
important
implications
advancing
therapeutic
strategies
medicine.
Stem Cells,
Journal Year:
2022,
Volume and Issue:
41(2), P. 105 - 110
Published: Sept. 25, 2022
Ovaries
are
central
to
development,
fertility,
and
reproduction
of
women.
A
particularly
interesting
feature
ovaries
is
their
accelerated
aging
compared
other
tissues,
leading
loss
function
far
before
organs
senesce.
The
limited
pool
ovarian
follicles
generated
birth
once
exhausted,
menopause
will
inevitably
commence
around
the
age
50
years
marking
end
fertility.
Yet,
there
reports
suggesting
presence
germline
stem
cells
neo-oogenesis
in
adult
human
ovaries.
These
observations
have
fueled
a
long
debate,
created
experimental
fertility
treatments,
opened
business
opportunities.
Our
recent
analysis
cell
types
cortex
women
fertile
could
not
find
evidence
cells.
Like
before,
our
work
has
been
met
with
critique
methodological
shortcomings.
We
agree
that
excellence
starts
methods
welcome
discussion
on
pros
cons
different
protocols.
In
this
commentary,
we
discuss
re-interpretation
work.
Reproductive Biology and Endocrinology,
Journal Year:
2024,
Volume and Issue:
22(1)
Published: July 23, 2024
Abstract
Reproductive
aging
not
only
affects
the
fertility
and
physical
mental
health
of
women
but
also
accelerates
process
other
organs.
There
is
an
urgent
need
newfor
novel
mechanisms,
targets,
drugs
to
break
vicious
cycle
mitochondrial
dysfunction,
redox
imbalance,
germ
cell
apoptosis
associated
with
ovarian
aging.
Autophagy,
recognized
as
a
longevity
mechanism,
has
recently
become
focal
point
in
anti-aging
research.
Although
mitophagy
type
autophagy,
its
role
regulatory
mechanisms
aging,
particularly
age-related
function
decline,
remain
unclear.
Nerve
growth
factor
inducible
gene
B
(Nur77)
early
response
that
can
be
stimulated
by
oxidative
stress,
DNA
damage,
metabolism,
inflammation.
Recent
evidence
recommends
decreased
expression
Nur77
myocardial
fibrosis,
renal
Parkinson's
disease;
however,
association
been
studied
yet.
We
herein
identified
regulator
senescence,
apoptosis,
found
overexpression
activate
mitophagy,
improve
reduce
ultimately
enhance
reserve
aged
mice
ovaries.
Furthermore,
we
discovered
between
AKT
pathway
through
String
molecular
docking
analyses.
Experimental
confirmation
revealed
AKT/mTOR
signaling
involved
regulation
function.
In
conclusion,
our
results
suggest
promising
target
for
preventing
treating
decline
related
reproductive
PLoS ONE,
Journal Year:
2024,
Volume and Issue:
19(10), P. e0311978 - e0311978
Published: Oct. 23, 2024
Primordial
follicle
activation
(PFA)
is
a
pivotal
event
in
female
reproductive
biology,
coordinating
the
transition
from
quiescent
to
growing
follicles.
This
study
employed
comprehensive
single-cell
RNA
sequencing
gain
insights
into
detailed
regulatory
mechanisms
governing
synchronized
dormancy
and
between
granulosa
cells
(GCs)
oocytes
with
progression
of
PFA
process.
Wntless
(
Wls
)
conditional
knockout
(cKO)
mice
served
as
unique
model,
suppressing
pre-GCs
GCs,
disrupting
somatic
cell-derived
WNT
signaling
ovary.
Our
data
revealed
immediate
transcriptomic
changes
GCs
post-PFA
cKO
mice,
leading
divergent
trajectory,
while
exhibited
modest
alterations.
Subpopulation
analysis
identified
molecular
pathways
affected
by
on
GC
maturation,
along
specific
gene
signatures
linked
dormant
activated
oocytes.
Despite
minimal
evidence
continuous
up-regulation
dormancy-related
genes
oocytes,
loss
(pre-)GCs
impacted
expression
even
before
PFA,
subsequently
influencing
them
globally.
The
infertility
observed
was
attributed
compromised
GC-oocyte
crosstalk
microenvironment
for
highlights
role
WNT-signaling
pathway
its
signature,
emphasizing
importance
intercellular
orchestrating
folliculogenesis.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Aug. 22, 2023
Ovulation
is
essential
for
reproductive
success,
yet
the
underlying
cellular
and
molecular
mechanisms
are
far
from
clear.
Here,
we
applied
high-resolution
spatiotemporal
transcriptomics
to
map
out
cell-type-
ovulation-stage-specific
programs
as
function
of
time
during
follicle
maturation
ovulation
in
mice.
Our
analysis
revealed
dynamic
transitions
within
granulosa
cell
types
that
occur
tight
coordination
with
mesenchymal
proliferation.
We
identified
new
markers
emerging
cumulus
fate
preantral-to-antral
transition.
describe
transcriptional
respond
rapidly
stimulation
those
associated
rupture,
highlighting
prominent
roles
apoptotic
metabolic
pathways
final
stages
maturation.
further
report
stage-specific
oocyte-cumulus
interactions
diverging
differentiation
follicles
approaching
ovulation.
Collectively,
this
study
provides
insights
into
processes
regulate
mouse
ovarian
important
implications
advancing
therapeutic
strategies
medicine.
