Metagenomic assembly insight into the antibiotic resistance genes and antibiotic resistant bacteria in packaged drinking water system

Journal of environmental chemical engineering, Journal Year: 2025, Volume and Issue: unknown, P. 115381 - 115381

Published: Jan. 1, 2025

Language: Английский

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Physical communication pathways in bacteria: an extra layer to quorum sensing

Biophysical Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Abstract Bacterial communication is essential for survival, adaptation, and collective behavior. While chemical signaling, such as quorum sensing, has been extensively studied, physical cues play a significant role in bacterial interactions. This review explores the diverse range of stimuli, including mechanical forces, electromagnetic fields, temperature, acoustic vibrations, light that bacteria may experience with their environment within community. By integrating these pathways, can coordinate activities adapt to changing environmental conditions. Furthermore, we discuss how stimuli modulate growth, lifestyle, motility, biofilm formation. understanding underlying mechanisms, develop innovative strategies combat infections optimize industrial processes.

Language: Английский

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A review of chemical signaling pathways in the quorum sensing circuit of Pseudomonas aeruginosa

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 254, P. 127861 - 127861

Published: Nov. 7, 2023

Language: Английский

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Life at the borderlands: microbiomes of interfaces critical to One Health

FEMS Microbiology Reviews, Journal Year: 2024, Volume and Issue: 48(2)

Published: Feb. 28, 2024

Abstract Microbiomes are foundational components of the environment that provide essential services relating to food security, carbon sequestration, human health, and overall well-being ecosystems. Microbiota exert their effects primarily through complex interactions at interfaces with plant, animal, hosts, as well within soil environment. This review aims explore ecological, evolutionary, molecular processes governing establishment function microbiome–host relationships, specifically critical One Health—a transdisciplinary framework recognizes health outcomes people, animals, plants, tightly interconnected. Within context Health, core principles underpinning microbiome assembly will be discussed in detail, including biofilm formation, microbial recruitment strategies, mechanisms attachment, community succession, effect these have on host health. Finally, this catalogue recent advances microbiology ecology methods can used profile interfaces, particular attention multi-omic, advanced imaging, modelling approaches. These technologies for delineating general specific functions, mapping interconnectivity across varying spatial temporal scales, predictive frameworks guide development targeted microbiome-interventions deliver Health outcomes.

Language: Английский

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Substrate stiffness modulates collective colony expansion of the social bacterium Myxococcus xanthus

APL Bioengineering, Journal Year: 2025, Volume and Issue: 9(1)

Published: Jan. 17, 2025

Many cellular functions depend on the physical properties of cell's environment. bacteria have different types surface appendages to enable adhesion and motion various surfaces. Myxococcus xanthus is a social soil bacterium with two distinctly regulated modes motility, termed motility mode, driven by type IV pili, adventurous based focal complexes. How sense surfaces subsequently coordinate their collective remains largely unclear. Using polyacrylamide hydrogels tunable stiffness, we found that wild M. spreads faster stiffer substrates. Here, show using mutants disrupt suppresses this substrate stiffness response, suggesting adhesion-based dependent. We also modifying adding adhesive ligands, chitosan, increases amount flairs, characteristic feature motility. Taken together, hypothesize central role as driving mechanism for sensing.

Language: Английский

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Study of Antimicrobial Resistance, Biofilm Formation, and Motility of Pseudomonas aeruginosa Derived from Urine Samples

Microorganisms, Journal Year: 2023, Volume and Issue: 11(5), P. 1345 - 1345

Published: May 19, 2023

Pseudomonas aeruginosa causes urinary tract infections associated with catheters by forming biofilms on the surface of indwelling catheters. Therefore, controlling spread bacteria is crucial to preventing its transmission in hospitals and environment. Thus, our objective was determine antibiotic susceptibility profiles twenty-five P. isolates from UTIs at Medical Center Trás-os-Montes Alto Douro (CHTMAD). Biofilm formation motility are also virulence factors studied this work. Out isolates, 16% exhibited multidrug resistance, being resistant least three classes antibiotics. However, showed a high prevalence amikacin tobramycin. Resistance carbapenem antibiotics, essential for treating when other antibiotics fail, low study, Notably, 92% demonstrated intermediate sensitivity ciprofloxacin, raising concerns about efficacy disease. Genotypic analysis revealed presence various β-lactamase genes, class B metallo-β-lactamases (MBLs) most common. The blaNDM, blaSPM, blaVIM-VIM2 genes were detected 16%, 60%, 12% strains, respectively. these highlights emerging threat MBL-mediated resistance. Additionally, gene varying rates among strains. exoU gene, cytotoxicity, found only one isolate, while such as exoS, exoA, exoY, exoT had prevalence. toxA lasB present all whereas lasA absent. suggests potential strains cause severe infections. This pathogen proficiency producing biofilms, be capable doing so. Currently, resistance serious public health problems, options become inadequate continued emergence multidrug-resistant combined rate biofilm production ease dissemination. In conclusion, study provides insights into isolated human urine infections, highlighting need surveillance appropriate therapeutic approaches.

Language: Английский

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Migration of surface-associated microbial communities in spaceflight habitats

Biofilm, Journal Year: 2023, Volume and Issue: 5, P. 100109 - 100109

Published: Feb. 24, 2023

Astronauts are spending longer periods locked up in ships or stations for scientific and exploration spatial missions. The International Space Station (ISS) has been inhabited continuously more than 20 years the duration of space stays by crews could lengthen with objectives human presence on moon Mars. If environment these habitats is designed comfort astronauts, it also conducive to other forms life such as embarked microorganisms. latter, most often associated surfaces form biofilm, have implicated significant degradation functionality pieces equipment habitats. recent research suggests that microgravity increase persistence, resistance virulence pathogenic microorganisms detected communities, endangering health astronauts potentially jeopardizing long-duration manned In this review, we describe mechanisms dynamics installation propagation microbial communities (spatial migration), well long-term processes adaptation evolution extreme environments (phenotypic genetic special reference health. We discuss means control envisaged allow a lasting cohabitation between vibrant microscopic passengers astronauts.

Language: Английский

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Material Substrate Physical Properties Control Pseudomonas aeruginosa Biofilm Architecture

mBio, Journal Year: 2023, Volume and Issue: 14(2)

Published: Feb. 14, 2023

In the wild, bacteria are most frequently found in form of multicellular structures called biofilms. Biofilms grow at surface abiotic and living materials with wide-ranging mechanical properties. The opportunistic pathogen Pseudomonas aeruginosa forms biofilms on indwelling medical devices soft tissues, including burn wounds airway mucosa. Despite critical role substrates foundation biofilms, we still lack a clear understanding how material mechanics regulate their architecture physiology resident bacteria. Here, demonstrate that physical properties hydrogel define P. biofilm architecture. We show mesh size regulates twitching motility, exploration mechanism priming ultimately controlling organization single cells community. resulting architectural transitions increase aeruginosa's tolerance to colistin, last-resort antibiotic. addition, regulation motility affects clonal lineages, so more mixed relatively denser materials. Our results thereby establish as factor dramatically architecture, antibiotic efficacy, evolution population.

Language: Английский

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Jammed microgel growth medium prepared by flash-solidification of agarose for 3D cell culture and 3D bioprinting

Biomedical Materials, Journal Year: 2023, Volume and Issue: 18(4), P. 045011 - 045011

Published: May 5, 2023

Although cells cultured in three-dimensional (3D) platforms are proven to be beneficial for studying cellular behavior settings similar their physiological state, due the ease, convenience, and accessibility, traditional 2D culturing approaches widely adopted. Jammed microgels a promising class of biomaterials extensively suited 3D cell culture, tissue bioengineering, bioprinting. However, existing protocols fabricating such either involve complex synthesis steps, long preparation times, or polyelectrolyte hydrogel formulations that sequester ionic elements from growth media. Hence, there is an unmet need broadly biocompatible, high-throughput, easily accessible manufacturing process. We address these demands by introducing rapid, remarkably straightforward method synthesize jammed composed flash-solidified agarose granules directly prepared culture medium choice. Our media optically transparent, porous, yield stress materials with tunable stiffness self-healing properties, which makes them ideal as well The charge-neutral inert nature make suitable various types species, specific do not alter chemistry Unlike several platforms, readily compatible standard techniques absorbance-based assays, antibiotic selection, RNA extraction, live encapsulation. In effect, we present versatile, highly accessible, inexpensive, adoptable biomaterial envision widespread application just routine laboratory but also designing multicellular mimics dynamic co-culture models niches.

Language: Английский

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Beyond Dipolar Activity: Quadrupolar Stress Drives Collapse of Nematic Order on Frictional Substrates

Physical Review Letters, Journal Year: 2025, Volume and Issue: 134(8)

Published: Feb. 26, 2025

The field of active nematics has traditionally employed descriptions based on dipolar activity. However, it is theoretically predicted that interactions with a substrate, prevalent in most biological systems, lead to novel forms activity, such as quadrupolar are governed by hydrodynamic screening. Here, combining experiments and numerical simulations, we show upon light-induced solidification the underlying medium, microtubule-kinesin mixtures undergo transformation leads biphasic suspension. Using an lyotropic model, prove transition screening effects alter dominant form stress. Specifically, combined effect friction activity hierarchical folding follows intrinsic bend instability nematic layer. Our results demonstrate dynamics collapse orientational order present new route for controlling matter modifying through changing surrounding environment.

Language: Английский

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