Smell and Aftersmell: Fast Calcium Imaging Dynamics of Honey Bee Olfactory Coding DOI Creative Commons
Marco Paoli, Antoine Wystrach,

Brice Ronsin

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 21, 2023

Abstract Odour processing exhibits multiple parallels between vertebrate and invertebrate olfactory systems. Insects, in particular, have emerged as relevant models for studies because of the tractability their circuits. Here, we used fast calcium imaging to track activity projection neurons honey bee antennal lobe (AL) during stimulation at high temporal resolution. We observed a heterogeneity response profiles an abundance inhibitory activities, resulting various latencies stimulus- specific post-odour neural signatures. Recorded signals were fed mushroom body (MB) model constructed implementing fundamental features connectivity neurons, Kenyon cells (KC), MB output (MBON). The accounts increase odorant discrimination compared AL reveals recruitment two distinct KC populations that represent odorants aftersmell separate but temporally coherent objects. Finally, showed learning- induced modulation KC-to-MBON synapses can explain both variations associative learning scores across different conditioning protocols bees bees’ latency. Thus, it provides simple explanation how time contingency stimulus reward be encoded without need tracking. This study broadens our understanding coding bees. It demonstrates based on rules with real physiological data aspects odour learning.

Language: Английский

The mesencephalic locomotor region recruits V2a reticulospinal neurons to drive forward locomotion in larval zebrafish DOI Creative Commons
Martin Carbó-Tano, Mathilde Lapoix, Xinyu Jia

et al.

Nature Neuroscience, Journal Year: 2023, Volume and Issue: 26(10), P. 1775 - 1790

Published: Sept. 4, 2023

Abstract The mesencephalic locomotor region (MLR) is a brain stem area whose stimulation triggers graded forward locomotion. How MLR neurons recruit downstream vsx2 + (V2a) reticulospinal (RSNs) poorly understood. Here, to overcome this challenge, we uncovered the locus of in transparent larval zebrafish and show that distinct from nucleus medial longitudinal fasciculus. stimulations reliably elicit locomotion controlled duration frequency. V2a RSNs via projections onto somata pontine retropontine areas, dendrites medulla. High-speed volumetric imaging neuronal activity reveals strongly MLR-coupled are active for steering or swimming, whereas weakly medullary encode frequency component. Our study demonstrates how specific control kinematics suggests conservation motor functions across vertebrates.

Language: Английский

Citations

22
Analysis of fast calcium dynamics of honey bee olfactory coding DOI Creative Commons
Marco Paoli, Antoine Wystrach,

Brice Ronsin

et al.

eLife, Journal Year: 2024, Volume and Issue: 13

Published: Sept. 5, 2024

Odour processing exhibits multiple parallels between vertebrate and invertebrate olfactory systems. Insects, in particular, have emerged as relevant models for studies because of the tractability their circuits. Here, we used fast calcium imaging to track activity projection neurons honey bee antennal lobe (AL) during stimulation at high temporal resolution. We observed a heterogeneity response profiles an abundance inhibitory activities, resulting various latencies stimulus-specific post-odour neural signatures. Recorded signals were fed mushroom body (MB) model constructed implementing fundamental features connectivity neurons, Kenyon cells (KC), MB output (MBON). The accounts increase odorant discrimination compared AL reveals recruitment two distinct KC populations that represent odorants aftersmell separate but temporally coherent objects. Finally, showed learning-induced modulation KC-to-MBON synapses can explain both variations associative learning scores across different conditioning protocols bees bees' latency. Thus, it provides simple explanation how time contingency stimulus reward be encoded without need tracking. This study broadens our understanding coding bees. It demonstrates based on rules with real physiological data aspects odour learning.

Language: Английский

Citations

4
Analysis of fast calcium dynamics of honey bee olfactory coding DOI Creative Commons
Marco Paoli, Antoine Wystrach,

Brice Ronsin

et al.

eLife, Journal Year: 2024, Volume and Issue: 13

Published: Feb. 15, 2024

Odour processing exhibits multiple parallels between vertebrate and invertebrate olfactory systems. Insects, in particular, have emerged as relevant models for studies because of the tractability their circuits. Here, we used fast calcium imaging to track activity projection neurons honey bee antennal lobe (AL) during stimulation at high temporal resolution. We observed a heterogeneity response profiles an abundance inhibitory activities, resulting various latencies stimulus-specific post-odour neural signatures. Recorded signals were fed mushroom body (MB) model constructed implementing fundamental features connectivity neurons, Kenyon cells (KC), MB output (MBON). The accounts increase odorant discrimination compared AL reveals recruitment two distinct KC populations that represent odorants aftersmell separate but temporally coherent objects. Finally, showed learning-induced modulation KC-to-MBON synapses can explain both variations associative learning scores across different conditioning protocols bees bees' latency. Thus, it provides simple explanation how time contingency stimulus reward be encoded without need tracking. This study broadens our understanding coding bees. It demonstrates based on rules with real physiological data aspects odour learning.

Language: Английский

Citations

2
Granger Causality Analysis of Transient Calcium Dynamics in the Honey Bee Antennal Lobe Network DOI Creative Commons
Marco Paoli, Yuri Antonacci, Angela Albi

et al.

Insects, Journal Year: 2023, Volume and Issue: 14(6), P. 539 - 539

Published: June 9, 2023

Odorant processing presents multiple parallels across animal species, and insects became relevant models for the study of olfactory coding because tractability underlying neural circuits. Within insect brain, odorants are received by sensory neurons processed antennal lobe network. Such a network comprises nodes, named glomeruli, that receive information interconnected local interneurons participating in shaping representation an odorant. The functional connectivity between nodes vivo is challenging task requires simultaneous recording from at high temporal resolutions. Here, we followed calcium dynamics glomeruli applied Granger causality analysis to assess among presence absence odorous stimulus. This approach revealed existence causal links stimulation, while odor arrival, network's density increased stimulus-specific. Thus, such analytical may provide new tool investigation plasticity vivo.

Language: Английский

Citations

4
Influence of microgravity on spontaneous calcium activity of primary hippocampal neurons grown in microfluidic chips DOI Creative Commons
Pierre-Ewen Lecoq,

Chloé Dupuis,

Xavier Mousset

et al.

npj Microgravity, Journal Year: 2024, Volume and Issue: 10(1)

Published: Feb. 6, 2024

The influence of variations gravity, either hypergravity or microgravity, on the brain astronauts is a major concern for long journeys in space, to Moon Mars, simply long-duration missions ISS (International Space Station). Monitoring activity, before and after already demonstrated important term effects brains astronauts. In this study, we focus gravity at cellular level primary hippocampal neurons. A dedicated setup has been designed built perform live calcium imaging during parabolic flights. During CNES (Centre National d'Etudes Spatiales) flight campaign, were able observe monitor activity 2D networks neurons inside microfluidic devices changes over different parabolas. Our preliminary results clearly indicate modification associated gravity.

Language: Английский

Citations

1
Fiber photometry in neuroscience research: principles, applications, and future directions DOI Creative Commons
Michał Kiełbiński, Joanna Bernacka

Pharmacological Reports, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 5, 2024

Language: Английский

Citations

1
Smell and Aftersmell: Fast Calcium Imaging Dynamics of Honey Bee Olfactory Coding DOI Creative Commons
Marco Paoli, Antoine Wystrach,

Brice Ronsin

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 21, 2023

Abstract Odour processing exhibits multiple parallels between vertebrate and invertebrate olfactory systems. Insects, in particular, have emerged as relevant models for studies because of the tractability their circuits. Here, we used fast calcium imaging to track activity projection neurons honey bee antennal lobe (AL) during stimulation at high temporal resolution. We observed a heterogeneity response profiles an abundance inhibitory activities, resulting various latencies stimulus- specific post-odour neural signatures. Recorded signals were fed mushroom body (MB) model constructed implementing fundamental features connectivity neurons, Kenyon cells (KC), MB output (MBON). The accounts increase odorant discrimination compared AL reveals recruitment two distinct KC populations that represent odorants aftersmell separate but temporally coherent objects. Finally, showed learning- induced modulation KC-to-MBON synapses can explain both variations associative learning scores across different conditioning protocols bees bees’ latency. Thus, it provides simple explanation how time contingency stimulus reward be encoded without need tracking. This study broadens our understanding coding bees. It demonstrates based on rules with real physiological data aspects odour learning.

Language: Английский

Citations

0