Male rats emit aversive 44-kHz ultrasonic vocalizations during prolonged Pavlovian fear conditioning DOI Creative Commons
Krzysztof H. Olszyński, Rafał Polowy, Agnieszka D. Wardak

et al.

eLife, Journal Year: 2024, Volume and Issue: 12

Published: Dec. 10, 2024

Rats are believed to communicate their emotional state by emitting two distinct types of ultrasonic vocalizations. The first is long ‘22-kHz’ vocalizations (>300 ms, <32-kHz) with constant frequency, signaling aversive states, and the second short ‘50-kHz’ calls (<150 >32 kHz), often frequency-modulated, in appetitive situations. Here, we describe emitted at a higher pitch male Wistar spontaneously hypertensive rats (SHR) an intensified – prolonged fear conditioning. These calls, which named ‘44-kHz’ vocalizations, (>150 ms), generally frequency (usually within 35–50-kHz range) have overall spectrographic image similar 22-kHz calls. Some 44-kHz comprised both 22-kHz-like 44-kHz-like elements. Furthermore, separate clustering methods confirmed that these can be separated from other We observed associated freezing behavior during conditioning training, they constituted up 19.4% all most them appeared next each forming uniform groups (bouts). also show some rats’ responses playback were more akin for example, heart rate changes, whereas intermediate level between Our results suggest wider vocal repertoire than previously believed, current definitions major call may require reevaluation. hope future investigations rat models human diseases will contribute expanding our understanding therapeutic strategies related psychiatric conditions.

Language: Английский

Biphasic Npas4 expression promotes inhibitory plasticity and suppression of fear memory consolidation in mice DOI Creative Commons
David V.C. Brito, Janina Kupke, Rostislav A. Sokolov

et al.

Molecular Psychiatry, Journal Year: 2024, Volume and Issue: 29(7), P. 1929 - 1940

Published: Feb. 13, 2024

Long-term memories are believed to be encoded by unique transcriptional signatures in the brain. The expression of immediate early genes (IEG) promotes structural and molecular changes required for memory consolidation. Recent evidence has shown that brain is equipped with mechanisms not only promote, but actively constrict formation. However, it remains unknown whether IEG may play a role suppression. Here we uncovered novel function neuronal PAS domain protein 4 (Npas4), as an inducible suppressor gene highly salient aversive experiences. Using contextual fear conditioning paradigm, found low stimulus salience leads monophasic Npas4 expression, while learning induces biphasic hippocampus. later phase requires N-methyl-D-aspartate (NMDA) receptor activity independent dopaminergic neurotransmission. Our vivo pharmacological genetic manipulation experiments suggested restricts consolidation promote behavioral flexibility, facilitating extinction specificity responses. Moreover, immunofluorescence electrophysiological analysis revealed concomitant increase synaptic input from cholecystokinin (CCK)-expressing interneurons. results demonstrate how experiences evoke temporal patterns fine-tune our study provides associated suppression possible mechanism balance memories, thereby evade formation maladaptive behavior.

Language: Английский

Citations

8
Cortico-subcortical networks that determine behavioral memory renewal are redefined by noradrenergic neuromodulation DOI Creative Commons
Josué Haubrich,

Laura Dolón Vera,

Denise Manahan‐Vaughan

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 20, 2025

Abstract During spatial appetitive extinction learning (EL), rodents learn that previously rewarded behavior is no longer rewarded. Renewal of the extinguished enabled by re-exposure to context in which occurred. When renewal response (RR) unrewarded, it rapidly followed (RE). Although hippocampus known be engaged, whether this dynamic supported different brain networks unclear. To clarify this, male rats engaged context-dependent memory acquisition, EL and RR testing a T-Maze. Fluorescence situ hybridization disambiguated somatic immediate early gene expression neuronal somata or RE. Graph analysis revealed pronounced hippocampal connectivity with retrosplenial prefrontal cortex (PFC) during initial RR. By contrast, RE was accompanied shift towards elevated coordinated activity within all subfields. Given β-adrenergic receptors (β-AR) regulate memory, we activated β-AR further scrutinize these network effects. This enhanced prevented Effects were associated initially increased thalamic-hippocampus activity, decrease intraconnectivity predominance PFC. Our findings highlight critical hippocampal-cortical-thalamic underpins behavior, noradrenergic neuromodulation playing pivotal role governing circuit’s dynamics.

Language: Английский

Citations

0
Dual-step pharmacological intervention for traumatic-like memories: implications from D-cycloserine and cannabidiol or clonidine in male and female rats DOI
Luciane A. Soares, Laura Maria Martins Nascimento, Francisco Silveira Guimarães

et al.

Psychopharmacology, Journal Year: 2024, Volume and Issue: 241(9), P. 1827 - 1840

Published: May 1, 2024

Language: Английский

Citations

3
Network-level changes in the brain underlie fear memory strength DOI Creative Commons
Josué Haubrich, Karim Nader

eLife, Journal Year: 2023, Volume and Issue: 12

Published: June 27, 2023

The strength of a fear memory significantly influences whether it drives adaptive or maladaptive behavior in the future. Yet, how mild and strong memories differ underlying biology is not well understood. We hypothesized that this distinction may be exclusively result changes within specific brain regions, but rather outcome collective connectivity across multiple regions neural network. To test this, rats were conditioned protocols varying intensities to generate memories. Neuronal activation driven by recall was measured using c-fos immunohistochemistry 12 implicated learning memory. interregional coordinated activity computed graph-based functional networks generated compare at systems level. Our results show supported well-connected network with small-world properties which amygdala well-positioned modulated other regions. In contrast, disrupted isolated from These findings indicate differ, implications for understanding treating disorders dysregulation.

Language: Английский

Citations

6
Male rats emit aversive 44-kHz ultrasonic vocalizations during prolonged Pavlovian fear conditioning DOI Creative Commons
Krzysztof H. Olszyński, Rafał Polowy, Agnieszka D. Wardak

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: June 23, 2023

Rats are believed to communicate their emotional state by emitting two distinct types of ultrasonic vocalizations. The first is long ‘22-kHz’ vocalizations (>300 ms, <32-kHz) with constant frequency, signaling aversive states, and the second short ‘50-kHz’ calls (<150 >32 kHz), often frequency-modulated, in appetitive situations. Here, we describe emitted at a higher pitch male Wistar spontaneously hypertensive rats (SHR) an intensified – prolonged fear conditioning. These calls, which named ‘44-kHz’ vocalizations, (>150 ms), generally frequency (usually within 35–50-kHz range) have overall spectrographic image similar 22-kHz calls. Some 44-kHz comprised both 22-kHz-like 44-kHz-like elements. Furthermore, separate clustering methods confirmed that these can be separated from other We observed associated freezing behavior during conditioning training, they constituted up 19.4% all most them appeared next each forming uniform groups (bouts). also show some rats’ responses playback were more akin for example, heart rate changes, whereas intermediate level between Our results suggest wider vocal repertoire than previously believed, current definitions major call may require reevaluation. hope future investigations rat models human diseases will contribute expanding our understanding therapeutic strategies related psychiatric conditions.

Language: Английский

Citations

2
Male rats emit aversive 44-kHz ultrasonic vocalizations during prolonged Pavlovian fear conditioning DOI Creative Commons
Krzysztof H. Olszyński, Rafał Polowy, Agnieszka D. Wardak

et al.

eLife, Journal Year: 2024, Volume and Issue: 12

Published: Dec. 10, 2024

Rats are believed to communicate their emotional state by emitting two distinct types of ultrasonic vocalizations. The first is long ‘22-kHz’ vocalizations (>300 ms, <32-kHz) with constant frequency, signaling aversive states, and the second short ‘50-kHz’ calls (<150 >32 kHz), often frequency-modulated, in appetitive situations. Here, we describe emitted at a higher pitch male Wistar spontaneously hypertensive rats (SHR) an intensified – prolonged fear conditioning. These calls, which named ‘44-kHz’ vocalizations, (>150 ms), generally frequency (usually within 35–50-kHz range) have overall spectrographic image similar 22-kHz calls. Some 44-kHz comprised both 22-kHz-like 44-kHz-like elements. Furthermore, separate clustering methods confirmed that these can be separated from other We observed associated freezing behavior during conditioning training, they constituted up 19.4% all most them appeared next each forming uniform groups (bouts). also show some rats’ responses playback were more akin for example, heart rate changes, whereas intermediate level between Our results suggest wider vocal repertoire than previously believed, current definitions major call may require reevaluation. hope future investigations rat models human diseases will contribute expanding our understanding therapeutic strategies related psychiatric conditions.

Language: Английский

Citations

0