Effects of focused ultrasound in a “clean” mouse model of ultrasonic neuromodulation

iScience, Journal Year: 2023, Volume and Issue: 26(12), P. 108372 - 108372

Published: Nov. 1, 2023

Highlights•A "clean" mouse model minimizes off-target auditory effects of UNM•Increasing FUS pressure induces thermal and/or non-auditory confounds•Intense elicits spreading and destructive brain depolarization•Direct cortical activation is not observed with the tested parametersSummaryRecent studies on ultrasonic neuromodulation (UNM) in rodents have shown that focused ultrasound (FUS) can activate peripheral pathways, leading to brain-wide excitation, which obscures direct target area by FUS. To address this issue, we developed a new model, double transgenic Pou4f3+/DTR × Thy1-GCaMP6s, allows for inducible deafening using diphtheria toxin UNM while allowing neural activity be visualized fluorescent calcium imaging. Using found confounds caused significantly reduced or eliminated within certain range. At higher pressures, result focal fluorescence dips at target, elicit sensory confounds, damage tissue, depolarization. Under acoustic conditions tested, did observe responses cortex. Our findings provide cleaner animal sonogenetics research, establish parameter range are confidently avoided, reveal side higher-pressure stimulation.Graphical abstract

Language: Английский

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High-throughput ultrasound neuromodulation in awake and freely behaving rats

Brain stimulation, Journal Year: 2023, Volume and Issue: 16(6), P. 1743 - 1752

Published: Nov. 1, 2023

Transcranial ultrasound neuromodulation is a promising potential therapeutic tool for the noninvasive treatment of neuropsychiatric disorders. However, expansive parameter space and difficulties in controlling peripheral auditory effects make it challenging to identify sequences brain targets that may provide efficacy. Careful preclinical investigations clinically relevant behavioral models are critically needed suitable acoustic parameters. there lack devices allowing multi-target experimental awake unrestrained rodents. We developed miniaturized 64-element array enables neurointerventional with within-trial active control freely behaving rats. first characterized field free-water transcranial propagation measurements. then confirmed vivo can target multiple regions via electronic steering, verified wearing device does not cause any significant impairments animal motility. Finally, we demonstrated performance our system high-throughput experiment, where found stimulation rat central medial thalamus, but an target, promotes arousal increases locomotor activity.

Language: Английский

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Low Intensity Pulsed Ultrasound Activates Excitatory Synaptic Networks in Cultured Hippocampal Neurons

Ultrasound in Medicine & Biology, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

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Spinal Cord Ultrasound Stimulation Modulates Corticospinal Excitability in Humans

Brain stimulation, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Low-intensity focused ultrasound offers deep tissue penetration and holds promise as a noninvasive tool for neuromodulating brain circuits. However, its effects on neural activity within the human spinal cord remain largely unexplored. To investigate of stimulation (SCUS) corticospinal excitability by varying acoustic parameters: spatial-peak pulse-average intensity (ISPPA), duty cycle (DC), pulse repetition frequency (PRF). Two experiments were conducted involving 62 healthy adult participants. In Experiment 1 (N = 36), participants randomly assigned to either low-intensity group (2.5 W/cm2, N 18) or relatively high-intensity (10 examine parameter-dependent SCUS effects. was delivered 500 ms train C8 segment using two DCs (10% 30%) PRFs (500 1000 Hz). Stimulation applied concurrently with posterior-anterior (PA) oriented transcranial magnetic (TMS) over left primary motor cortex (M1), motor-evoked potentials (MEPs) recorded from right first dorsal interosseous (FDI) muscle. Active sham trials interleaved compared. 2 26), specificity assessed in relation TMS coil orientation target muscle sonication parameter set 10 W/cm2 ISPPA, Hz PRF, 30% DC. MEPs FDI anterior-posterior (AP) latero-medial (LM) orientations, while abductor digiti minimi (ADM) PA, AP, LM orientations. also biceps brachii PA orientation. conditions significantly suppressed MEP amplitudes compared under W/cm2), high-PRF (1000 Hz) conditions, regardless No significant observed at lower W/cm2) PRF Follow-up confirmed consistent inhibitory across multiple orientations (PA, LM) C8-innervated hand muscles (FDI ADM), no modulation more rostrally innervated muscle, supporting C8-targeted SCUS. These findings demonstrate that can modulate parameter-specific manner, suppression primarily conditions. represents promising technique targeted neuromodulation circuits humans.

Language: Английский

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Interactive Effect Between Transcranial Focused Ultrasound and Transcranial Magnetic Stimulation on Human Motor Cortex

Clinical Neurophysiology, Journal Year: 2024, Volume and Issue: 167, P. 92 - 105

Published: Sept. 11, 2024

Language: Английский

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