LC-MS Profiling Reveals Metabolic Dynamics in <em>Apis mellifera</em> Worker Bee Larvae–Pupae Transition DOI Open Access
Zhen Li, Shiqing Zhong, Bin Liu

et al.

Published: May 24, 2024

Metabolites present in the hemolymph of honey bees play a crucial role modulating metamorphic process within species. However, precise alterations metabolite composition, along with associated variances and regulatory pathways implicated during larvae-to-pupae metamorphosis bees, remain incompletely elucidated. In this investigation, we gathered samples from bee larvae at three distinct physiological stages-feeding, prepupal, pupae subjected them to analysis utilizing liquid chromatography-mass spectrometry (LC-MS) technique. Employing principal component (PCA) partial least squares-discriminant (PLS-DA), identified significant differential metabolites conducted metabolic pathway on those differentially up-regulated prepupal stages. Notably, stage primarily governed glucose metabolism fat digestion absorption, while were involved regulating chitin lipopolysaccharide precursor formation, as well biosynthesis phenylalanine, tyrosine, tryptophan. These findings bear implications for advancing our comprehension processes bees.

Language: Английский

Solanaceae Glycoalkaloids Disturb Lipid Metabolism in the Tenebrio molitor Beetle DOI Creative Commons
Magdalena Joanna Winkiel, Szymon Chowański, Marek Gołębiowski

et al.

Metabolites, Journal Year: 2023, Volume and Issue: 13(12), P. 1179 - 1179

Published: Nov. 30, 2023

Glycoalkaloids (GAs) are produced naturally by plants and affect insect survivability fertility. These compounds can be considered potential bioinsecticides; however, the mechanisms effects of their action remain undiscovered. As lipids essential molecules for proper functioning an organism, this research aimed to determine GAs on lipid metabolism Tenebrio molitor beetle. Solanine, chaconine, tomatine, tomato leaf extract were applied larvae injection at two concentrations, 10-8 10-5 M. Then, tissue was isolated after 2 24 h levels free fatty acids, sterols esters using GC-MS technique. Moreover, triacylglyceride level activity key β-oxidation enzyme, 3-hydroxyacyl-CoA dehydrogenase (HADH), measured. The results indicate that content composition in beetles' haemolymph fat body. depend GA incubation time, kind tissue. tested decrease HADH activity, especially body, which may energy production. To our knowledge, is first study concerning T. application. Our provide some insights into topic.

Language: Английский

Citations

5
Effects of temperature adjustment with a heating device in weak honey bee colonies in cold seasons DOI
İbrahim Çakmak,

Basri Kul,

Faten Ben Abdelkader

et al.

International Journal of Biometeorology, Journal Year: 2023, Volume and Issue: 67(11), P. 1765 - 1774

Published: Aug. 16, 2023

Language: Английский

Citations

4
Changes in honey bee nutrition after exposure to radiofrequency electromagnetic field DOI Creative Commons
Paweł Migdał, Mateusz Plotnik, Paweł Bieńkowski

et al.

The European Zoological Journal, Journal Year: 2024, Volume and Issue: 91(1), P. 172 - 179

Published: Jan. 2, 2024

Urban beekeeping has gained interest in recent years. Bees placed cities, on the roofs of buildings, are exposed to many different sources electromagnetic fields frequencies and intensities. Knowledge about impact physiology behavior insects (including honey bees) is limited. Hence, one first steps was check how radiofrequency affect bees' nutrition. The level total proteins, glucose, triglycerides, TAS activity bee hemolymph analyzed. This because they indicate bee's nutrition level. experiment involved 2-day-old Carniolan worker bees (Apis mellifera carnica L.). were 12 V/m, 28 61 V/m for 0.25, 1 3 h. Hemolymph taken from 40 alive each group immediately after exposure, by removing antennae with sterile tweezers. biochemical markers (total TAS, triglycerides) groups showed statistically significant differences compared control most cases. According our own research, disturb changes analyzed indicators may have long-term effects.

Language: Английский

Citations

1
Development of an artificial diet with a mass-rearing and low-cost approach for Spodoptera frugiperda reproduction DOI
Jaime González‐Cabrera, Yadira Contréras-Bermúdez, Jorge Antonio Sánchez‐González

et al.

International Journal of Tropical Insect Science, Journal Year: 2024, Volume and Issue: 44(3), P. 1195 - 1205

Published: April 6, 2024

Language: Английский

Citations

1
LC-MS Profiling Reveals Metabolic Dynamics in <em>Apis mellifera</em> Worker Bee Larvae–Pupae Transition DOI Open Access
Zhen Li, Shiqing Zhong, Bin Liu

et al.

Published: May 24, 2024

Metabolites present in the hemolymph of honey bees play a crucial role modulating metamorphic process within species. However, precise alterations metabolite composition, along with associated variances and regulatory pathways implicated during larvae-to-pupae metamorphosis bees, remain incompletely elucidated. In this investigation, we gathered samples from bee larvae at three distinct physiological stages-feeding, prepupal, pupae subjected them to analysis utilizing liquid chromatography-mass spectrometry (LC-MS) technique. Employing principal component (PCA) partial least squares-discriminant (PLS-DA), identified significant differential metabolites conducted metabolic pathway on those differentially up-regulated prepupal stages. Notably, stage primarily governed glucose metabolism fat digestion absorption, while were involved regulating chitin lipopolysaccharide precursor formation, as well biosynthesis phenylalanine, tyrosine, tryptophan. These findings bear implications for advancing our comprehension processes bees.

Language: Английский

Citations

1