Horn size is linked to Sertoli cell efficiency and sperm size homogeneity during sexual development in common eland (Taurotragus oryx) DOI Creative Commons
Eliana Pintus, Radim Kotrba, José Luis Ros‐Santaella

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: Aug. 20, 2024

Background: In polygynous species, the development of secondary sexual characters is usually decisive for male reproductive success. However, our understanding about links between growth these traits and efficiency still elusive. Most research efforts in this topic have been also focused on adult males, although some characters, like bovid horns, typically starts after birth, continues during puberty such as common eland, slows or even stops adulthood. study, we investigated relationships horn size testicular function elands using a comprehensive approach that considers both spermatogenic sperm parameters. Methods: Twenty-two non-sexually mature were used present study. Horn size, body mass, testes gonadosomatic index assessed. Spermatogenic activity was determined by cytological histological analyses. Sperm concentration, morphology, morphometry, intramale variation evaluated epididymal samples. Cluster analysis performed to explore influence age function. Results: We found bigger horns are associated with increased Sertoli cell reduced size. Both parameters not related one another while they shown be enhanced quality ungulates. Moreover, positively linked testis investment seminiferous epithelium. Spiral length basal circumference most strongly correlated well those responsible dimorphism species. rendered two groups: first including males ≤30 months old, second >30 old. within groups, strongest relationship homogeneity Conclusion: Taken together, results indicate can regarded good potential provide insights into role selection dynamics.

Language: Английский

Current status and potential of morphometric sperm analysis DOI Creative Commons
José Julián Garde,

Alejandro Maroto-Morales,

Olga García‐Álvarez

et al.

Asian Journal of Andrology, Journal Year: 2016, Volume and Issue: 18(6), P. 863 - 863

Published: Jan. 1, 2016

The spermatozoon is the most diverse cell type known and this diversity considered to reflect differences in sperm function. How morphology arose during speciation what role different specializations play function, however, remain incompletely characterized. This work reviews hypotheses proposed explain morphological evolution, with a focus on some aspects of morphometric evaluation; ability morphometrics predict cryoresistance male fertility also discussed. For this, evaluation patterns change head morphometry throughout process, instead study characteristics at stages, allows better identification males cryoconservation ability. These new approaches, together more studies employing greater number individuals, are needed obtain novel results concerning Future should aim understanding causes design mechanisms that generate them, giving increased attention other structures besides head. implementation scientific technological advances could benefit simultaneous examination phenotype demonstrating be useful tool for assessment.

Language: Английский

Citations

42
CORRELATION BETWEEN THE MARAL STAGS HORMONAL STATUS AND THE SPERM PRODUCTS QUALITY OBTAINED IN THE RUTTING SEASON DOI

Andrey Vyacheslavovich Boranbaev,

Aleksey Nepriyatel'

Bulletin of KSAU, Journal Year: 2025, Volume and Issue: 0(6), P. 125 - 129

Published: Jan. 22, 2025

The purpose of research is to study the relationship between hormonal status stag deer and quality resulting sperm production during rut. Objectives: determine level testosterone cortisol in blood rut period levels. Research was carried out Novotalitsk branch Federal State Budgetary Scientific Institution FANCA (the Altai Region) 2022. object were (n=10) aged 6–9 years with elite, first second class. selection bioproducts from autumn rutting season, using a mobile machine for fixing when semen taken. Sperm obtained by method electroejaculation according established regimens; before taking, prepuce toilet performed. collected sterile receptacles, then its qualitative quantitative assessment out. At same time, taken hormones. ranges 1.25–13.153 nmol/l, – 35.00–260.00 nmol/l. There change levels depending on class animals. In stags elite concentration hormone above 3.943 ± 2.20 nmol/l rut, an ejaculate volume 3.5–7.0 ml observed, activity up 9 points 700 million 1.0 billion/ml.

Language: Английский

Citations

0
High-energy diet enhances spermatogenic function and increases sperm midpiece length in fallow deer ( Dama dama ) yearlings DOI Creative Commons
José Luis Ros‐Santaella, Radim Kotrba, Eliana Pintus

et al.

Royal Society Open Science, Journal Year: 2019, Volume and Issue: 6(6), P. 181972 - 181972

Published: June 1, 2019

Nutrition is a major factor involved in the sexual development of livestock ruminants. In male, high-energy diet enhances reproductive function, but its effects on underlying processes such as spermatogenic efficiency are not yet defined. Moreover, possible changes sperm size due to supplemented remain poorly investigated. The main goal this study was evaluate whether affects activity, epididymal parameters (concentration, morphology, morphometry and acrosome integrity) blood testosterone levels fallow deer yearlings. For purpose, 32 were allocated into two groups according their diet: control (pasture) experimental (pasture barley grain) groups. Fallow from group showed significant increase Sertoli cell function midpiece length, together with higher testicular mass, concentration percentage normal spermatozoa than (p < 0.05). We also found tendency for animals fed grain = 0.116). better quality may be related cells an earlier onset puberty. results present work elucidate mechanisms by which dietary supplementation male might useful practices management seasonal breeders.

Language: Английский

Citations

18
New Sperm Morphology Analysis in Equids: Trumorph® Vs Eosin-Nigrosin Stain DOI Creative Commons
Sabrina Gacem, Jaime Catalán, Iván Yánez-Ortiz

et al.

Veterinary Sciences, Journal Year: 2021, Volume and Issue: 8(5), P. 79 - 79

Published: May 6, 2021

The evaluation of the male fertility potential is based on analysis basic spermatic characteristics concentration, motility and morphology. Thus, study sperm morphology a fundamental element in seminal analysis, but its real meaning has been biased by techniques used for evaluation. These involve dehydration phases subsequent staining, which involves production artifacts. aim to compare two methods equid semen evaluation, Trumorph® using living vs. eosin-nigrosine stain. A total 49 ejaculates from stallions donkeys were used. After collection dilution, an aliquot was placed slide introduced device. Then observation made with 40x objective negative phase-contrast microscope. Another stained stain viewed 100× magnification. Well-formed observed, different abnormalities identified Trumorph®. use eosin-nigrosin staining method led same results both can be stallion donkey morphological analysis. However, considering fact that uses helps prevent cell alteration during sample preparation. Therefore, good alternative conventional method, provides quick test live sperm.

Language: Английский

Citations

8
Spermatozoa morphometry and ultrastructure in estuarine crocodile (Crocodylus porosus) DOI Creative Commons
Wan‐Nor Fitri, Abd Wahid Haron,

PutraTengku Rinalfi

et al.

Asian Pacific Journal of Reproduction, Journal Year: 2020, Volume and Issue: 9(2), P. 104 - 104

Published: Jan. 1, 2020

Objective: To evaluate normal spermatozoa morphometry and ultrastructure in estuarine crocodile (Crocodylus porosus).Methods: Four adult male crocodiles aged between 12-15 years, with an average snout to tail length of (3.15±0.01) m were selected for this study. Manipulation the phallus digitally from base penis was performed on four facilitate flow semen sulcus. Semen collected all individuals Morphometry analysis eosin-nigrosin stained sample. Scanning electron microscopy conducted observe surface spermatozoa.Results: The morphology made up acrosome, head, which corresponded (5.55±1.20) μm, (12.74±1.57) (70.67±4.40) respectively. total measured at (88.96±0.52) μm. most common abnormalities found samples cytoplasmic droplets.Conclusions: Normal morphology, are vermiform shaped-head a long along questionably high droplets count described

Language: Английский

Citations

6
Testicular histology of Anurans that deposit eggs out of water DOI Creative Commons

Ana Pucci Alcaide,

Franco Pucci Alcaide,

Adriana Azucena Michel

et al.

Acta Zoológica Lilloana, Journal Year: 2020, Volume and Issue: unknown, P. 84 - 115

Published: Dec. 3, 2020

Anuran amphibians show the largest diversity of amphibian reproductive modes, many which imply egg deposition out water. This kind requires specialisations to avoid desiccation. Physiological, anatomical and ethological traits integrate define these modes. In particular, morphological features urogenital system correlate with modes environmental conditions where egg-laying occurs. this study, we describe testicular histology spermatogenesis nest building frog Leptodactylus latinasus, compare it other species that breed We found variations in tes tis size, thickness interstitial tissue, tunica albuginea, peritubular tunics, flagellum length, shape size spermatozoal nucleus. Certain specifics’ characters differed at level, but not between families. Such variation could be an indicator performance constraints under fertilisation takes place.

Language: Английский

Citations

5
Spermatogenic Activity and Sperm Traits in Post-Pubertal and Adult Tomcats (Felis catus): Implication of Intra-Male Variation in Sperm Size DOI Creative Commons
Eliana Pintus, Martin Kadlec,

Barbora Karlasová

et al.

Cells, Journal Year: 2021, Volume and Issue: 10(3), P. 624 - 624

Published: March 11, 2021

Tomcats are considered to be adults at 1 year of age, although many reach sexual maturity an earlier age. Nevertheless, we still know little about whether the spermatogenic activity and sperm quality mature under one-year-old tomcats differ from those that over one-year-old. This study aims evaluate activity, traits, their relationships in two different ages. Sixteen showing complete spermatogenesis spermatozoa epididymal caudae were used classified according age as post-pubertal (<1 old) or adult (˃1 old). Our results show cats have higher concentration lower coefficient variation head width ellipticity than cats. However, they do not testicular mass, spermatogenesis, traits such motility, mitochondrial morphology, morphometry, well plasma membrane, acrosome, DNA integrity. Reduced intra-male is associated with testis epididymis concentration. Interestingly, low size increased Sertoli cell function reduced post-meiotic germ loss. These findings increase our knowledge feline reproductive physiology provide new insights into functional significance shape tomcats.

Language: Английский

Citations

5
Horn size is linked to Sertoli cell efficiency and sperm size homogeneity during sexual development in common eland (Taurotragus oryx) DOI Open Access
Eliana Pintus, Radim Kotrba, José Luis Ros‐Santaella

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 5, 2024

Abstract Background In polygynous species, the development of secondary sexual characters is usually decisive for male reproductive success. However, our understanding about links between growth these traits and efficiency still elusive. Most research efforts in this topic have been also focused on adult males, although some characters, like bovid horns, typically starts after birth, continues during puberty such as common eland, slows or even stops adulthood. study, we investigated relationships horn size testicular function elands using a comprehensive approach that considers both spermatogenic sperm parameters. Methods Twenty-two non-sexually mature were analyzed present study. Horn was estimated by performing principal component analysis length, spiral basal circumference. addition, body mass, testes gonadosomatic index assessed. Spermatogenic activity assessed cytological histological analyses. Sperm concentration, morphology, morphometry, intramale variation evaluated epididymal samples. Results We found bigger horns are associated with increased Sertoli cell reduced size. Both parameters not related to one another while they shown be enhanced quality ungulates. Moreover, positively linked testis investment seminiferous epithelium. Surprisingly, did correlate percentage spermatozoa over germ population nor those normal which increase throughout development. It remarkable note length circumference most strongly correlated well responsible dimorphism species. Conclusions Taken together, results indicate can regarded good potential provide insights into role selection dynamics.

Language: Английский

Citations

0
Horn size is linked to Sertoli cell efficiency and sperm size homogeneity during sexual development in common eland (Taurotragus oryx) DOI Creative Commons
Eliana Pintus, Radim Kotrba, José Luis Ros‐Santaella

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: Aug. 20, 2024

Background: In polygynous species, the development of secondary sexual characters is usually decisive for male reproductive success. However, our understanding about links between growth these traits and efficiency still elusive. Most research efforts in this topic have been also focused on adult males, although some characters, like bovid horns, typically starts after birth, continues during puberty such as common eland, slows or even stops adulthood. study, we investigated relationships horn size testicular function elands using a comprehensive approach that considers both spermatogenic sperm parameters. Methods: Twenty-two non-sexually mature were used present study. Horn size, body mass, testes gonadosomatic index assessed. Spermatogenic activity was determined by cytological histological analyses. Sperm concentration, morphology, morphometry, intramale variation evaluated epididymal samples. Cluster analysis performed to explore influence age function. Results: We found bigger horns are associated with increased Sertoli cell reduced size. Both parameters not related one another while they shown be enhanced quality ungulates. Moreover, positively linked testis investment seminiferous epithelium. Spiral length basal circumference most strongly correlated well those responsible dimorphism species. rendered two groups: first including males ≤30 months old, second &gt;30 old. within groups, strongest relationship homogeneity Conclusion: Taken together, results indicate can regarded good potential provide insights into role selection dynamics.

Language: Английский

Citations

0