High temperature increases centromere-mediated genome elimination frequency and enhances haploid induction in Arabidopsis

Plant Communications, Journal Year: 2022, Volume and Issue: 4(3), P. 100507 - 100507

Published: Dec. 20, 2022

Double haploid production is the most effective way to create true-breeding lines in a single generation. In Arabidopsis, induction via mutation of centromere-specific histone H3 (cenH3) has been shown when mutant outcrossed wild-type, and wild-type genome remains progeny. However, factors that affect are still poorly understood. Here, we report cenH3 assembly factor Kinetochore Null2 (KNL2) can be used as inducer pollinated by wild-type. We discovered short-term temperature stress knl2 increased efficiency 10-fold. also demonstrated point CENPC-k motif KNL2 sufficient generate haploid-inducing lines, suggesting crops identified naturally occurring or chemically induced population, avoiding generic modification (GM) approach at any stage. Furthermore, cenh3-4 functioned response heat stress, even though it did not induce haploids under standard conditions. Thus, new target gene for generation haploid-inducer showed exposure centromeric protein mutants high strongly increases their efficiency.

Language: Английский

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Effect of Curcumin Supplement in Summer Diet on Blood Metabolites, Antioxidant Status, Immune Response, and Testicular Gene Expression in Hu Sheep

Animals, Journal Year: 2019, Volume and Issue: 9(10), P. 720 - 720

Published: Sept. 24, 2019

In summer, the high temperature affects animal growth and reproductive performance. Curcumin is a flavonoid with anti-oxidant anti-inflammatory effects. To evaluate effects of dietary curcumin supplement on blood biochemical parameters testicular gene expressions in Hu sheep total 144 male aged four months were randomly divided into three groups (Con, Cur1, Cur2, n = 48). Sheep Con, Cur2 fed basal diet 0, 450, 900 mg (per sheep) daily, respectively. for 35 days, including pre-feed seven days. The results showed that 450 increased serum free fatty acid (NEFA) glutathione peroxidase (GPX), as well IgA IgM. IgG level, while had lower level than (p < 0.05). Dietary organ index, testosterone star mRNA expression Furthermore, linearly inhibited apoptosis bcl-2 decreased caspase-3 conclusion, can promote lipid metabolism, antioxidant capacity, immune response, development, sheep, which provides evidence application production.

Language: Английский

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HOS1 activates DNA repair systems to enhance plant thermotolerance

Nature Plants, Journal Year: 2020, Volume and Issue: 6(12), P. 1439 - 1446

Published: Nov. 16, 2020

Language: Английский

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Physiological mechanisms of stress-induced evolution

Journal of Experimental Biology, Journal Year: 2022, Volume and Issue: 225(Suppl_1)

Published: March 8, 2022

Organisms mount the cellular stress response whenever environmental parameters exceed range that is conducive to maintaining homeostasis. This critical for survival in emergency situations because it protects macromolecular integrity and, therefore, cell/organismal function. From an evolutionary perspective, counteracts severe by accelerating adaptation via a process called stress-induced evolution. In this Review, we summarize five key physiological mechanisms of Namely, these are changes in: (1) mutation rates, (2) histone post-translational modifications, (3) DNA methylation, (4) chromoanagenesis and (5) transposable element activity. Through each mechanisms, organisms rapidly generate heritable phenotypes may be adaptive, maladaptive or neutral specific contexts. Regardless their consequences individual fitness, produce phenotypic variation at population level. Because fuels natural selection, evolution increase likelihood populations can avoid extirpation instead adapt under new conditions.

Language: Английский

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Effect of heat stress on DNA damage: a systematic literature review

International Journal of Biometeorology, Journal Year: 2022, Volume and Issue: 66(11), P. 2147 - 2158

Published: Sept. 30, 2022

Language: Английский

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Identification of candidate regulators of the response to early heat stress in climate-adapted wheat landraces via transcriptomic and co-expression network analyses

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 3, 2024

Climate change is likely to lead not only increased global temperatures but also a more variable climate where unseasonal periods of heat stress are prevalent. This has been evidenced by the observation spring-time approaching 40°C in some main spring-wheat producing countries, such as USA, recent years. With an optimum growth temperature around 20°C, wheat particularly prone damage stress. A warming with increasingly common fluctuations therefore threatens crops and subsequently lives livelihoods billions people who depend on crop for food. To futureproof against climate, better understanding response early required. Here, we utilised DESeq2 identify 7,827 genes which were differentially expressed landraces after exposure. Candidate hub genes, may regulate transcriptional stress, identified via weighted gene co-expression network analysis (WGCNA), validated qRT-PCR. Two most promising candidate (TraesCS3B02G409300 TraesCS1B02G384900) downregulate expression involved drought, salinity, cold responses-genes unlikely be required under stress-as well photosynthesis hormone signalling repressors, respectively. We suggest role poorly characterised sHSP (TraesCS4D02G212300), activator response, potentially inducing vast suite shock proteins transcription factors known play key roles response. The present work represents exploratory examination heat-induced landrace seedlings identifies several act regulators this and, thus, targets breeders production thermotolerant varieties.

Language: Английский

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Thyroid‐Targeted Nano‐Bombs Empower HIFU for Graves' Disease

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Graves' disease (GD) is an autoimmune disorder with a high incidence rate, particularly affecting women of reproductive age. Current treatment modalities for GD carry significant disadvantages, especially pregnant or nursing women. As novel extracorporeal therapeutic technique, high-intensity focused ultrasound (HIFU) shows great promise treating GD; however, its low efficacy impedes clinical application. In this study, thyroid-targeted nano-bomb system (PD-PLGA@Si-Ab/PDA-I, PSAPI) developed to enhance HIFU and improve outcomes GD. The core structure PSAPI encapsulates phase-transition material, perfluorohexane, the anti-inflammatory drug diclofenac within poly(lactide-co-glycolide) (PLGA) silica shell. A polydopamine coating enhances biocompatibility, while iodine loading thyroid-stimulating hormone receptor (TSHR) antibodies grafting ensure targeted delivery thyroid. Robust in vitro vivo results demonstrated that highly biocompatible, accumulates thyroid 24 h after administration, significantly potentiates HIFU, resulting markedly reduced inflammatory responses. Transcriptomic analysis revealed cellular defense mechanism activated PSAPI-treated cells following irradiation, highlighting potential molecular targets future development HIFU-sensitizing agents. biocompatible study holds transformative potential, addressing critical gaps current practices

Language: Английский

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Generating transcriptomic resources in the teleost fish black cusk-eel (Genypterus maculatus) to evaluate thermal stress in the liver under a climate change scenario

Fish Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: 51(2)

Published: March 28, 2025

Language: Английский

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Involvement of RpoN in Regulating Motility, Biofilm, Resistance, and Spoilage Potential of Pseudomonas fluorescens

Frontiers in Microbiology, Journal Year: 2021, Volume and Issue: 12

Published: May 31, 2021

Pseudomonas fluorescens is a typical spoiler of proteinaceous foods, and it characterized by high spoilage activity. The sigma factor RpoN well-known regulator controlling nitrogen assimilation virulence in many pathogens. However, its exact role regulating the caused P . unknown. Here, an in-frame deletion mutation rpoN was constructed to investigate global regulatory function through phenotypic RNA-seq analysis. results assays showed that mutant deficient swimming motility, biofilm formation, resistance heat nine antibiotics, while increased H 2 O Moreover, markedly reduced extracellular protease total volatile basic (TVB-N) production sterilized fish juice at 4°C; meanwhile, with significantly higher sensory scores than wild-type strain. To identify RpoN-controlled genes, RNA-seq-dependent transcriptomics analysis strain performed. A 1224 genes were downregulated, 474 upregulated least two folds RNA level compared strain, revealing involvement several cellular processes, mainly flagellar mobility, adhesion, polysaccharide metabolism, resistance, amino acid transport metabolism; this may contribute stress antibiotic activities Our provide insights into food spoilage, which can be valuable ensure quality safety.

Language: Английский

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Modulating the Heat Stress Response to Improve Hyperthermia-Based Anticancer Treatments

Cancers, Journal Year: 2021, Volume and Issue: 13(6), P. 1243 - 1243

Published: March 12, 2021

Cancer treatments based on mild hyperthermia (39–43 °C, HT) are applied to a widening range of cancer types, but several factors limit their efficacy and slow down more widespread adoption. These include difficulties in adequate heat delivery, short therapeutic window the acquisition thermotolerance by cells. Here, we explore biological effects HT, cellular responses these clinically-relevant consequences. We then identify stress response—the defense mechanism that detects counteracts heat—as one major forces limiting HT-based therapies propose targeting this as potentially universal strategy for improving efficacy.

Language: Английский

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